scholarly journals Histologic and inflammatory lamellar changes in horses with oligofructose-induced laminitis treated with a CXCR1/2 antagonist

2016 ◽  
Vol 36 (1) ◽  
pp. 13-18 ◽  
Author(s):  
Leonardo R. de Lima ◽  
Heloisa M.F. Mendes ◽  
Frederico M. Soriani ◽  
Danielle G. de Souza ◽  
Geraldo Eleno S. Alves ◽  
...  
Keyword(s):  
Chemokine Receptors ◽  
Periodic Acid ◽  
Previous History ◽  
Basal Membrane ◽  
Treated Group ◽  
Periodic Acid Schiff ◽  
Chemokine Signaling ◽  
Mixed Breed ◽  
History Of ◽  
Membrane Changes

Abstract: With the hypothesis that blocking chemokine signaling can ameliorate acute laminitis, the aim was to evaluate the therapeutic effect of intravenous DF1681B, a selective antagonist for CXCR1 and CXCR2 (chemokine receptors), in an oligofructose equine laminitis model. To twelve mixed breed clinically healthy hoses with no previous history of hoof-related lameness was administered oligofructose (10g/kg given by nasogastric tube) and divided into two groups: treated (intravenous DF1681B at 30mg/kg 6, 12, 18, and 24h after oligofructose) and non-treated groups. Laminar biopsies were performed before and 12, 36, and 72h after administering oligofructose. Samples were stained with periodic acid-Schiff (PAS) and scored from 0 to 6 according to epidermal cell and basal membrane changes. The IL-1β, IL-6, and CXCL1 RNA expressions were determined by RT-PCR. Parametric and non-parametric tests were used to compare times within each group (P<0.05). The PAS grades and IL-1β and IL-6 RNA expression increased in the non-treated group, but remained constant in the treated horses. In conclusion, DF1681B therapy reduced laminar inflammation and epidermal deterioration in treated horses. CXCR1/2 blockage should be considered therapeutically for equine acute laminitis.

scholarly journals Scrofuloderma: A Diagnosis to Bear in Mind in the Western World

2019 ◽  
Vol 32 (4) ◽  
pp. 313
Author(s):  
Mariana Batista ◽  
Barbara Ferreira ◽  
Gonçalo Cruz ◽  
Américo Figueiredo
Keyword(s):  
Periodic Acid ◽  
Previous History ◽  
Complete Response ◽  
Purulent Discharge ◽  
Western World ◽  
Periodic Acid Schiff ◽  
Cutaneous Tuberculosis ◽  
Immunodeficiency Virus ◽  
History Of ◽  
Polymerase Chain

The incidence of tuberculosis has been increasing worldwide. Contrarily, a recent decrease in Portugal has been reported. Cutaneous tuberculosis comprises a low percentage of all cases. We report a 70-year-old female with a 2-month-history of painful, nodular, suppurative lesions in the groin area, bilaterally. Previous history was remarkable for Human Immunodeficiency Virus infection and stage-IIIB cervical cancer. A skin biopsy, stained with periodic acid–Schiff and Fite’s stain, polymerase chain reaction on purulent discharge and mycobacterial culture of the skin were performed, leading to the diagnosis of scrofuloderma. Tuberculostatic therapy was initiated and complete response was observed. This case depicts an uncommon variant of tuberculosis, highlighting the need for awareness of the cutaneous variants of tuberculosis that, although rare, can still present in the clinic today.

scholarly journals Candida Chorioamnionitis in Mothers with Gestational Diabetes Mellitus: A Report of Two Cases

2021 ◽  
Vol 18 (14) ◽  
pp. 7450
Author(s):  
Elia Shazniza Shaaya ◽  
Siti Atiqah Abdul Halim ◽  
Ka Wen Leong ◽  
Kevin Boon Ping Ku ◽  
Pei Shan Lim ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Gestational Diabetes ◽  
Gestational Diabetes Mellitus ◽  
Umbilical Cord ◽  
Neonatal Death ◽  
Periodic Acid ◽  
Careful Examination ◽  
Periodic Acid Schiff ◽  
History Of ◽  
Methenamine Silver

Background:Candida chorioamnionitis is rarely encountered, even though vulvovaginal candidiasis incidence is about 15%. Interestingly, it has characteristic gross and histological findings on the umbilical cord that are not to be missed. Case Report: We report two cases of Candida chorioamnionitis with presence of multiple yellowish and red spots of the surface of the umbilical cord. Microscopically, these consist of microabscesses with evidence of fungal yeasts and pseudohyphae. The yeasts and pseudohyphae were highlighted by periodic acid– Schiff and Grocott methenamine silver histochemical stains. Both cases were associated with a history of gestational diabetes mellitus. Discussion: Peripheral funisitis is a characteristic feature of Candida chorioamnionitis. It is associated with high risk of adverse perinatal and neonatal outcomes, such as preterm delivery, stillbirth and neonatal death. We recommend careful examination of the umbilical cord of mothers with gestational diabetes mellitus.

Dematiaceous fungal invasion of a bandage contact lens

2021 ◽  
Vol 14 (2) ◽  
pp. e240029
Author(s):  
Anirban Dutta ◽  
Sujata Das ◽  
Himanshu Sekhara Behera ◽  
Ruchi Mittal
Keyword(s):  
Contact Lens ◽  
Periodic Acid ◽  
Significant Risk ◽  
Dematiaceous Fungi ◽  
Periodic Acid Schiff ◽  
Bandage Contact Lens ◽  
History Of ◽  
Eye Examination ◽  
The Right

A 61-year-old man presented with a 1-month history of reduced vision, redness and pain in the right eye. Examination revealed a bandage contact lens (BCL) in situ with diffuse, pigmented deposits. On removal, the underlying cornea was found to be clear. He had been prescribed the BCL 6 months ago following a deep-seated corneal foreign body removal and was unable to follow-up subsequently.The BCL was sent for microbiological and histopathological evaluation. The culture revealed growth of Cladosporium spp, a dematiaceous fungi. Periodic acid–Schiff staining revealed infiltration of pigmented fungal filaments into the substance of the BCL.While contact lens deposits are a frequent finding, fungal deposits are seldom noted. Irregular follow-up and improper lens maintenance are significant risk factors for the same. Early identification and subsequent removal of the lens is vital to prevent infection of the underlying ocular structures.

scholarly journals GAMBARAN MIKROSKOPIK GINJAL TIKUS WISTAR (RATTUS NORVEGICUS) SETELAH DIINDUKSI DENGAN GENTAMISIN

2013 ◽  
Vol 4 (3) ◽  
Author(s):  
Poppy M Lintong ◽  
Carla F Kairupan ◽  
Priska L N Sondakh
Keyword(s):  
Body Weight ◽  
Epithelial Cells ◽  
Wistar Rats ◽  
Periodic Acid ◽  
Basal Membrane ◽  
Tubular Epithelial Cells ◽  
Periodic Acid Schiff ◽  
Group Iii ◽  
Group I ◽  
Group Ii

Abstract: Gentamycin, a frequently used aminoglycoside antibiotics, has a nephrotoxic effect to human beings and animals. The purpose of this research was to find out the microscopic changes of wistar rat kidneys after gentamycin induction. This was an experimental study, using five adult wistar rats, divided into three groups. Group I was the control group; group II consisted of two rats, injected with gentamycin 0,3 ml/day (dose of 60 mg/kg body weight/day) intraperitoneally for seven days; and group III consisted of two rats, injected with gentamycin 0,3 ml/day intraperitoneally for 10 days. Group I and II were terminated at day-8, and group III at day-11. Their kidneys were processed for microscopic slides, stained with hematoxylin eosin and Periodic Acid Schiff. In microscopic evaluation, group II and III showed oedema, necrosis, apoptosis, and basal membrane destruction of tubular epithelial cells. Group III also showed fat vacuoles in these epithelial cells (macrovesicular fatty changes). Conclusion: wistar rats injected with gentamycin 60 mg/kg body weight/day for 7 and 10 days showed oedema, necrosis, apoptosis, and basal membrane destruction of tubular epithelial cells; and macrovesicular fatty changes after 10 days of gentamycin.Key words: gentamycin, necrosis tubular epithelial cells, fatty changesAbstrak: Gentamisin termasuk antibiotik golongan aminoglikosida berspektrum luas yang bersifat nefrotoksik terhadap manusia dan hewan. Tujuan penelitian ini untuk melihat perubahan mikroskopik struktur ginjal tikus Wistar setelah diberikan gentamisin. Metode penelitian eksperimental dengan menggunakan lima ekor tikus Wistar dewasa yang dibagi atas tiga kelompok. Kelompok I tanpa perlakuan; kelompok II terdiri dari dua ekor tikus perlakuan yang diinjeksi dengan gentamisin 0,3 ml/hari (dosis 60 mg/kgBB/hari) secara intraperitonial selama tujuh hari; dan kelompok III terdiri dari dua ekor tikus perlakuan yang diinjeksi dengan gentamisin 0,3 ml/hari secara intraperitonial selama 10 hari. Tikus Wistar kelompok I dan II diteminasi hari ke-8, sedangkan kelompok III diterminasi hari ke-11. Ginjal tikus kelompok I -III kemudian dibuat preparat histopatologik dengan pengecatan rutin hematoksilin eosin dan Periodic Acid Schiff (PAS). Hasil penelitian menunjukkan tikus Wistar perlakuan yang diberikan gentamisin 0,3 ml/hari selama 7 sampai 10 hari secara mikroskopik memperlihatkan pembengkakan, nekrosis, apoptosis, dan destruksi membrana basalis sel epitel tubulus; dan pada hari ke-10 terlihat vakuol-vakuol lemak pada sel epitel sehingga inti terdesak ke tepi (perlemakan makrovesikuler). Simpulan: pemberian gentamisin pada tikus Wistar dengan dosis 60 mg/kg BB/hari selama 7-10 hari menunjukkan pembengkakan, nekrosis, apoptosis sel epitel tubulus, dan membrana basalis tubulus rusak; dan setelah hari ke-10 juga terlihat perlemakan makrovesikuler.Kata kunci: gentamisin, nekrosis sel epitel tubulus, perlemakan makrovesikuler

Plasma Cell Leukemia: A Report on Three Patients

1983 ◽  
Vol 69 (6) ◽  
pp. 589-591 ◽  
Author(s):  
Leonardo Pacilli ◽  
Paolo Ferraro ◽  
Silvia Cochi ◽  
Antonio De Laurenzi
Keyword(s):  
Plasma Cell ◽  
Peripheral Blood ◽  
Plasma Cells ◽  
Periodic Acid ◽  
Previous History ◽  
Bone Marrow Aspiration ◽  
Plasma Cell Leukemia ◽  
Cell Leukemia ◽  
Periodic Acid Schiff ◽  
The Third

Three patients with plasma cell leukemia are reported. Two of them had a previous history of myeloma; the third one started with a plasma cell leukemia. Diagnosis was made from the required presence of 20% plasma cells in the peripheral blood. In all 3 cases, bone marrow aspiration and peripheral blood showed plasma cells strongly positive for acid phosphatase and alpha-naphthyl acetate esterase, and negative for periodic acid-Schiff. The first patient was treated with a polychemotherapy regimen that included vincristine, cyclophosphamide, chlorambucil and prednisone, and the second patient with melphalan and prednisone; the third one, who started with plasma cell leukemia, received total body irradiation at the dose of 600 rad. The results of the therapy and survival time, which was never more than 3 months, are in accord with other reports in the literature.

scholarly journals Drug-related talaromycosis: A case report

2020 ◽  
Vol 34 ◽  
pp. 205873842093461
Author(s):  
Yongsheng Lu ◽  
Quan Shi ◽  
Jing Yu
Keyword(s):  
Drug Abuse ◽  
Periodic Acid ◽  
Fungal Spores ◽  
Public Health Problem ◽  
Giant Cells ◽  
Pathological Examination ◽  
Human Immune System ◽  
Periodic Acid Schiff ◽  
History Of ◽  
Anti Fungal

Talaromycosis is a rare deep fungal infection caused by Talaromyces marneffei. Currently, methamphetamine has become the second-largest drug abuse category in the world after cannabis and has become a serious public health problem. Methamphetamine can inhibit human immune system and increase the probability of pathogenic microorganism infection. On 8 October 2016, a 20-year-old man with a fever history of 2 months was admitted to our hospital. He had bloody stools and abdominal pain during hospitalization. There was no significant abnormality in physical examination. Because of the misdiagnosis, he underwent improper treatment. Periodic acid-Schiff stain (PAS) staining showed that the mucosa of distal ileum, ascending colon, transverse colon, and sigmoid colon were infiltrated by a large number of tissue cells, which contained a large number of blue purple particles. In addition, a large number of histiocytes and multinucleated giant cells can be seen in the lamina propria of ileum mucosa, and fungal spores can be seen in histiocytes. Finally, he was diagnosed as talaromycosis and took itraconazole 0.2 g twice a day. After 5 days, the temperature dropped to normal and the inflammation disappeared, and he continued to take itraconazole for 6 months. Due to the neglect of the history of drug abuse and the concealment, drug-related talaromycosis is often misdiagnosed. Pathological examination is warranted for diagnosis talaromycosis. This condition requires a long-term anti-fungal therapy.

scholarly journals Relationship between the morphologic alterations of vocal cords from adult autopsies and the cause of death

2004 ◽  
Vol 59 (2) ◽  
pp. 63-66 ◽  
Author(s):  
Ana Karina Marques Salge ◽  
Eumenia Costa da Cunha Castro ◽  
Mara Lúcia Fonseca Ferraz ◽  
Marlene Antônia dos Reis ◽  
Vicente de Paula Antunes Teixeira
Keyword(s):  
Vocal Cord ◽  
Cause Of Death ◽  
Respiratory Diseases ◽  
Periodic Acid ◽  
Basal Membrane ◽  
Vocal Cords ◽  
Periodic Acid Schiff ◽  
White Ethnicity ◽  
Staining Methods ◽  
Hematoxylin Eosin

PURPOSE: The purpose of this study was to identify the possible alteration in the thickness of the epithelium basal membrane of the vocal cords and correlate it with the cause of death. METHOD: Larynxes collected from adult autopsies during the period of 1993 to 2001 were utilized. We used the hematoxylin-eosin and periodic acid-Schiff staining methods for the morphological and morphometric analysis. RESULTS: Sixty-six vocal cords were analysed; increased thickness was identified in 14 cases (21.2%), with equal proportions between the genders. Increased vocal-cord thickness was more frequent in patients of the white ethnicity (12 cases, 85.7%). Respiratory alterations were found in 10 (71.4%) of the cases with increased vocal-cord thickness. Of the patients that were maintained with mechanical ventilation before death, 7 (18.4%) had thickening of the basal membrane. Among the smokers, 9 (19.63%) had basal membrane thickening. CONCLUSION: No statistically significant differences were found between the cases in which the cause of death was related to respiratory diseases as compared to non-respiratory diseases and the thickening of the basal membrane of the vocal cords. However, new studies are needed in order to verify the etiopathogenesis of this thickening.

Crystal-Storing Histiocytosis as a Cause of Symptomatic Cardiac Mass

2009 ◽  
Vol 133 (11) ◽  
pp. 1861-1864 ◽  
Author(s):  
Charles J. Sailey ◽  
Borislav A. Alexiev ◽  
James S. Gammie ◽  
Paula Pinell-Salles ◽  
J. Lawrence Stafford ◽  
...  
Keyword(s):  
Monoclonal Gammopathy ◽  
Plasma Cells ◽  
Periodic Acid ◽  
Right Atrial ◽  
Prior History ◽  
Low Grade ◽  
Periodic Acid Schiff ◽  
Right Atrial Mass ◽  
History Of

Abstract Crystal-storing histiocytosis is a rare disorder that is typically associated with low-grade B-cell lymphomas and monoclonal gammopathy. We present a 64-year-old man with a prior history of weakness and weight loss and hematologic evaluation that had revealed immunoglobulin G κ monoclonal light chains in the serum and negative bone marrow biopsy. He presented with supraventricular tachyarrhythmia and a right atrial mass seen on echocardiogram and excised surgically. Histologically, the tumor was composed of sheets of macrophages infiltrating the atrial myocardium. The histiocytes were filled with multiple needle-shaped, periodic acid-Schiff–negative crystals. These cells and associated plasma cells failed to show clonal light chain restriction by in situ hybridization or immunohistochemistry, and there was no area of lymphoma in the tumor. Ultrastructural examination showed numerous sticklike, trapezoidal, or polygonal dense crystals in the cytoplasm of histiocytes corroborating the diagnosis of crystal-storing histiocytosis. Although rare, crystal-storing histiocytosis should be included in the differential diagnosis of heart masses in patients with hematologic conditions associated with monoclonal gammopathy.

THE VALUE OF HYPOSENSITIZATION THERAPY FOR BRONCHIAL ASTHMA IN CHILDREN—A 14-YEAR STUDY

PEDIATRICS ◽  
1968 ◽  
Vol 42 (5) ◽  
pp. 793-802
Author(s):  
Douglas E. Johnstone ◽  
Arthur Dutton
Keyword(s):  
Bronchial Asthma ◽  
Dose Group ◽  
Clinical Course ◽  
Age Of Onset ◽  
Previous History ◽  
Treated Group ◽  
Controlled Study ◽  
History Of ◽  
Asthma In Children ◽  
Prospective Controlled Study

The clinical course of 210 children with perennial bronchial asthma was followed in a prospective controlled study in which one half of the children received placebo injections while the other half received conventional hyposensitization therapy. Of the 130 children still under observation at the time of their sixteenth birthday, 22% of the placebo-treated children were free of asthma compared to 72% of the treated children. In the treated group the rate of loss of asthma may be related to the dose of antigen received in hyposensitization therapy. Whereas 66% of the "1/5,000" group were free of asthma at the end of the study, 78% of the "highest tolerated dose" group were symptom free in their sixteenth year. The likelihood of a child outgrowing asthma was not significantly influenced by his sex, age of onset, or severity of his symptoms when first seen. A previous history of hay fever increased the likelihood of a child's asthma persisting into adolescence.

Actinomycosis of bilateral psoas muscle: a case report

MedPharmRes ◽  
2021 ◽  
Vol 5 (2) ◽  
pp. 7-11
Author(s):  
Hoang Huu Bui ◽  
Thao Thi Phuong Doan ◽  
Tuan Ngoc Cao ◽  
Tuong Trung Dinh ◽  
Chuong Dinh Nguyen
Keyword(s):  
Intrauterine Device ◽  
Periodic Acid ◽  
Pelvic Mass ◽  
Psoas Muscle ◽  
Invasive Disease ◽  
Periodic Acid Schiff ◽  
Muscle Involvement ◽  
History Of ◽  
Left Psoas Muscle ◽  
The Right

Actinomycosis is an underreported chronic invasive disease caused by Actinomyces spp. In that group of diseases, pelvic actinomycosis is very rare and most of the cases reported in the literature have shown unilateral psoas muscle involvement. We report a 35-year-old woman with a retroperitoneal mass located in the left psoas muscle that makes abdominal tenderness and difficulty in walking. She had a 7-year history of intrauterine device which had been removed one month just before this event. After undergoing two laparotomies to perform a biopsy with undetermined results, she developed a new mass in the right psoas muscle which was similar to the left one. On the third attempt, the histopathology revealed yellow sulfur granules of Actinomyces which reacted positively with periodic acid Schiff and Grocott’s dye. After three months of treatment with Amoxicillin plus Clavulanic acid, her abscesses were completely resolved. Actinomycosis should always be included in the diagnostic list in patients with a pelvic mass and a history of intrauterine device.

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