Vaccine Cold Chain: Part 1. Proper Handling and Storage of Vaccine

ABSTRACTThe diptheria outbreak in Surabaya indicated that immunization program failure. Immunization is primary preventif effort to decrease morbidity of disease. An immunization service is very important to protect vaccine quality through cold chain so that vaccine potency be optimal. The purpose of this study was to analysis of factors which are assosiated with midwive’s practice of DPT vaccine distribution and storage to outreach. This study is applying observational approach using cross sectional method. Populations are all village midwives in public health center East Surabaya. The numbers of sample were 38 midwives taken using simple random sampling. The dependent variable was midwive’s practice of DPT vaccine distribution and storage to outreach and the independent variables of this study were work duration, a history of training of cold chain, sosialization, knowledge, attitude. Primary data were obtained through observation and interview. The results showed that 68.4 % midwive’s practice on DPT vaccine distribution and storage at outreach is good. Independent variable which are significant assosiated with midwive’s practice on DPT vaccine distribution and storage at outreach is sosialization about vaccine distribution and storage (p = 0.026) and value of phi and Cramer’s V = 0.431. Enhancement of socialization again be needed to village midwive as efforts for increase knowledge and attitude.Keywords: midwive, cold chain, vaccine distribution, DPT

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Vaccine Cold Chain: Part 1. Proper Handling and Storage of Vaccine

AAOHN Journal ◽

10.3928/08910162-20100801-03 ◽

2010 ◽

Vol 58 (8) ◽

pp. 345-346

Keyword(s):

Cold Chain ◽

And Storage

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Cold Chain Logistics Management of Medicine with an Integrated Multi-Criteria Decision-Making Method

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph16234843 ◽

2019 ◽

Vol 16 (23) ◽

pp. 4843 ◽

Cited By ~ 6

Author(s):

Zhi Wen ◽

Huchang Liao ◽

Ruxue Ren ◽

Chunguang Bai ◽

Edmundas Kazimieras Zavadskas ◽

...

Keyword(s):

Decision Making ◽

Multiple Criteria Decision Making ◽

Cold Chain ◽

Solution Methods ◽

Criteria Weights ◽

Ranking Of Alternatives ◽

And Storage ◽

Selection Of

Medicine is the main means to reduce cancer mortality. However, some medicines face various risks during transportation and storage due to the particularity of medicines, which must be kept at a low temperature to ensure their quality. In this regard, it is of great significance to evaluate and select drug cold chain logistics suppliers from different perspectives to ensure the quality of medicines and reduce the risks of transportation and storage. To solve such a multiple criteria decision-making (MCDM) problem, this paper proposes an integrated model based on the combination of the SWARA (stepwise weight assessment ratio analysis) and CoCoSo (combined compromise solution) methods under the probabilistic linguistic environment. An adjustment coefficient is introduced to the SWARA method to derive criteria weights, and an improved CoCoSo method is proposed to determine the ranking of alternatives. The two methods are extended to the probabilistic linguistic environment to enhance the applicability of the two methods. A case study on the selection of drug cold chain logistics suppliers is presented to demonstrate the applicability of the proposed integrated MCDM model. The advantages of the proposed methods are highlighted through comparative analyses.

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Hubungan Karakteristik dan Tingkat Pengetahuan Petugas Imunisasi terhadap Praktik Penyimpanan dan Transportasi Vaksin Imunisasi di Tingkat Puskesmas Kota Padang Tahun 2014

Jurnal Kesehatan Andalas ◽

10.25077/jka.v4i3.386 ◽

2015 ◽

Vol 4 (3) ◽

Author(s):

Nadia Rahmah ◽

Putri Sri Lasmini ◽

Rahmatini Rahmatini

Keyword(s):

Health Care ◽

Statistical Tests ◽

Health Centers ◽

Cold Chain ◽

Good Knowledge ◽

Training Experience ◽

Cross Sectional ◽

Handling Practices ◽

And Storage ◽

And Training

Abstrak Rantai dingin sangat penting dipertahankan selama distribusi dan penyimpanan vaksin untuk mencapai efektifitas vaksin. Petugas Imunisasi di layanan primer harus memiliki pengetahuan dan pemahaman yang baik mengenai transportasi dan penyimpanan vaksin. Penelitian dilaksanakan di seluruh puskesmas Kota Padang padaMaret 2014, dengan menggunakan desain cross sectional. Jumlah sampel adalah 21petugas imunisasi dengan teknik total sampling. Pengumpulan data dilakukan dengan menggunakan kuesioner dan observasi. Hasil penelitian didapatkan responden dengan pengetahuan yang baik tentang penyimpanan dan transportasi vaksin sebesar 61,9% dan praktik penyimpanan dan transportasi vaksin di Puskesmas yang baik sebesar 61,9%. Berdasarkan uji statistik, didapatkan tidak adanya hubungan umur, tingkat pendidikan, masa kerja dan pengalaman pelatihan petugas imunisasi dengan praktik penyimpanan dan transportasi vaksin di Puskesmas tetapi terdapat hubungan pengetahuan petugas dengan praktik. Berdasarkan hasil penelitian disimpulkan bahwa tidak terdapatnya hubungan karakteristik petugas imunisasi dengan praktik penyimpanan dan transportasi vaksin dan terdapatnya hubungan bermakna antarapengetahuan petugas dengan praktik penyimpanan dan transportasi vaksin di Puskesmas Kota Padang.Kata kunci: karakteristik, pengetahuan, penyimpanan, transportasi vaksinAbstract Preserving the cold chain during distribution and storage is critical to achieve the effectiveness of the vaccine. Immunization workers in primary health care should have a good knowledge and understanding about the handling and storage of the vaccine. The experiment was conducted in all health centers Padang in 2014 March, using a crosssectional design. Total samples 21 immunization workers with total sampling. The data was collected using questionnaires and observation. The results showed respondents with good knowledge about vacci ne storage and handling of 61.9% and vaccine storage and handling practices in health centers of 61.9% which is good. Based on statistical tests, found no rellation between age, education, years of working and training experience of immunizationworkers with vaccine storage and handling practices in health care but a significant correlation between knowledge with practice. Based on the results of the study concluded that the absence of the relations between characteristic immunization workers with vaccine storage and handling practices and a significant correlation between knowledge workers with practical storage and handling of vaccines at the health center of Padang.Keywords: characteristics, knowledge, storage, handling of vaccines

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Heat Transfer Parameterization towards Enhancing Shelf Life of Vegetables in Low Cost Cold Chain with FACCC

International Journal of Innovative Technology and Exploring Engineering - Special Issue ◽

10.35940/ijitee.d1106.029420 ◽

2020 ◽

Vol 9 (4) ◽

pp. 822-829

Keyword(s):

Heat Transfer ◽

Nusselt Number ◽

Shelf Life ◽

Temperature Control ◽

Low Cost ◽

Surface Heat ◽

Cold Chain ◽

Air Flow Rate ◽

Surface Heat Transfer Coefficient ◽

And Storage

India is the second-largest vegetable producing country after China. It is observed that there is a huge loss of vegetables due to the lack of low-cost cold chain for its storage & transportation after harvesting from agriculture land to vegetable market. The shelf life of these vegetables is less due to poor temperature control during transportation and storage. If vegetables are not maintained at their prudent temperature, then they will lose their potency, which will lead to enormous economic loss. Therefore, it is observed that temperature control of vegetables and their heat transfer characteristics are a major factor to maintain potency and increase their shelf life. This paper proposed a low-cost design of FACCC for temperature control of vegetables during transportation and storage with the help of Chillers. Further, the effect of air velocity on heat transfer rate and Nusselt number of various vegetables was evaluated to predict the performance of FACCC for enhancing the shelf life of vegetables.Thermophysical characteristics of these vegetables have been calculated at different air flow rate with the help of Reynolds analogy as a function of Stanton Number, Reynold’s Number and Prandtl Number.The airflow rate was maintained between 0.2065 m/s - 0.413 m/s inside FACCC. Experimental study reveals that the average Nusselt Number of these vegetables lies in the range of 57.9 - 115.74 and the range of Surface heat transfer coefficient is 54.48-108.96 W/m2K. Validation of FACCC performance has been done by comparing the heat transfer rate and Nusselt number from published literature. These results are obtained by temperature control of vegetables by varying air flow rate through four fans fitted inside FACCC. It results in higher shelf life due to favourable maintenance of thermodynamic parameters such as surface heat transfer coefficient, Nusselt number, Reynold number and preferable temperature for specific commodities.Thus, the proposed FACCC model can be an effective tool for enhancement of low-cost cold chain for vegetables in future.

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SARS-CoV-2 antibody binding and neutralization in dried blood spot eluates and paired plasma

10.1101/2021.08.02.21261504 ◽

2021 ◽

Author(s):

Hannah L. Itell ◽

Haidyn Weight ◽

Carolyn S. Fish ◽

Jennifer K. Logue ◽

Nicholas Franko ◽

...

Keyword(s):

Dried Blood Spots ◽

Antibody Binding ◽

Antibody Responses ◽

Cold Chain ◽

Correlates Of Protection ◽

Antibody Assays ◽

Plasma Antibody ◽

Highly Correlated ◽

And Storage ◽

Blood Spot

Widescale assessment of SARS-CoV-2-specific antibodies is critical to understanding population seroprevalence, correlates of protection, and the longevity of vaccine-elicited responses. Most SARS-CoV-2 studies characterize antibody responses in plasma/sera. While reliable and broadly used, these samples pose several logistical restrictions such as requiring venipuncture for collection and cold chain for transportation and storage. Dried blood spots (DBS) overcome these barriers as they can be self-collected by fingerstick and mailed and stored at ambient temperature. Here, we evaluate the suitability of DBS for SARS-CoV-2 antibody assays by comparing several antibody responses between paired plasma and DBS from SARS-CoV-2 convalescent and vaccinated individuals. We found that DBS not only reflected plasma antibody binding by ELISA and epitope profiles using phage-display, but also yielded SARS-CoV-2 neutralization titers that highly correlated with paired plasma. Neutralization measurement was further streamlined by adapting assays to a high-throughput 384-well format. This study supports the adoption of DBS for numerous SARS-CoV-2 binding and neutralization assays.

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DNA Extract was from filter paper stored at room temperature v1

10.17504/protocols.io.buuunwww ◽

2021 ◽

Author(s):

Katie Izenour ◽

Anwar Kalalah ◽

Fayez Salib ◽

Sarah not provided Zohdy

Keyword(s):

Filter Paper ◽

Whole Blood ◽

Room Temperature ◽

Cold Chain ◽

Western World ◽

Blood Collection ◽

Conventional Pcr ◽

Liquid Form ◽

And Storage ◽

Babesia Spp

Preservation of samples requiring cold-chain storage is an often unavoidable challenge especially when doing laboratory work outside the western world. Samples are a precious commodity and it is imperative to maintain their viability. One method for collection and storage of samples in a liquid form (blood, saliva, serum) at room temperature is on filter paper cards like the Cellabs Tropbio Filter Paper Blood Collection Disks™. Methods and protocols exist for the use and recovery of whole blood from filter paper discs. This protocol describes the wash and recovery of DNA extracted from whole blood using the Qiagen DNeasy Extraction kit and dried on filter paper, re-suspended after several months of room temperature storage and use in conventional PCR. This protocol was used to suspend DNA extracted from the wholeblood of Dogs in Cairo, Egypt. The DNA was extracted using the Qiagen DNEasy Extraction Kit and placed on Cellabs Tropbio Filter Paper Blood Collection Disks™ (filter paper). The DNA dried on the filter paper overnight and was stored in plastic self-closure baggies for several months before being washed off and used in Conventional PCR. The use case described here is of a dog who was PCR positive for Babesia spp. using a sample of whole blood washed from the filter paper as well as dried DNA from filter paper.

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IMPORTANCE OF COLD CHAIN MAINTENANCE AND STORAGE TEMPERATURE TO AVOCADO RIPENING AND QUALITY

Acta Horticulturae ◽

10.17660/actahortic.2011.911.65 ◽

2011 ◽

pp. 555-564 ◽

Cited By ~ 2

Author(s):

R.J. Blakey ◽

J.P. Bower ◽

I. Bertling

Keyword(s):

Storage Temperature ◽

Cold Chain ◽

And Storage

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Development of vaccine formulations: past, present, and future

Drug Delivery and Translational Research ◽

10.1007/s13346-021-00924-7 ◽

2021 ◽

Author(s):

Carmine D’Amico ◽

Flavia Fontana ◽

Ruoyu Cheng ◽

Hélder A. Santos

Keyword(s):

Cold Chain ◽

Alternative Formulation ◽

Vaccine Formulation ◽

Administration Route ◽

Formulation Development ◽

Advantages And Disadvantages ◽

The Past ◽

Near Future ◽

And Storage ◽

Conventional Vaccine

AbstractThe current situation, heavily influenced by the ongoing pandemic, puts vaccines back into the spotlight. However, the conventional and traditional vaccines present disadvantages, particularly related to immunogenicity, stability, and storage of the final product. Often, such products require the maintenance of a “cold chain,” impacting the costs, the availability, and the distribution of vaccines. Here, after a recall of the mode of action of vaccines and the types of vaccines currently available, we analyze the past, present, and future of vaccine formulation. The past focuses on conventional formulations, the present discusses the use of nanoparticles for vaccine delivery and as adjuvants, while the future presents microneedle patches as alternative formulation and administration route. Finally, we compare the advantages and disadvantages of injectable solutions, nanovaccines, and microneedles in terms of efficacy, stability, and patient-friendly design. Graphical abstract Different approaches to vaccine formulation development, the conventional vaccine formulations from the past, the current development of lipid nanoparticles as vaccines, and the near future microneedles formulations are discussed in this review.

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