Assessment of monocyte esterase activity by flow cytophotometry.
1976 ◽
Vol 24
(1)
◽
pp. 363-372
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Keyword(s):
Azo Dye
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An azo dye supravital method has been devised for selectively staining human monocytes in suspension for nonspecific esterase activity. Stained cells can be identified and rapidly enumerated by presenting the suspension of stained cells to the Cytograf, a flow-through cell discriminating cytophotometer. The intensity of stain is proportional to the intracellular esterase activity. By analysis of the oscilloscope display, it has been possible to obtain relative data concerning the degree of activity of monocyte nonspecific esterase activity. These observations suggest a unique approach to the measurement of intracellular enzyme activity in selected cells in a mixed population.
1977 ◽
Vol 25
(7)
◽
pp. 590-596
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1977 ◽
Vol 68
(2)
◽
pp. 273-275
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1983 ◽
Vol 157
(3)
◽
pp. 843-861
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1970 ◽
Vol 18
(4)
◽
pp. 291-301
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1967 ◽
Vol 45
(9)
◽
pp. 1375-1384
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1981 ◽
Vol 37
(9)
◽
pp. 1026-1027
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1988 ◽
Vol 111
(1)
◽
pp. 147-151
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