scholarly journals ADAP deficiency impairs megakaryocyte polarization with ectopic proplatelet release and causes microthrombocytopenia

Blood ◽  
2018 ◽  
Vol 132 (6) ◽  
pp. 635-646 ◽  
Author(s):  
Markus Spindler ◽  
Judith M. M. van Eeuwijk ◽  
Yvonne Schurr ◽  
Paquita Nurden ◽  
Bernhard Nieswandt ◽  
...  
Keyword(s):  
Life Span ◽  
Membrane System ◽  
Key Points ◽  
Platelet Release

Key Points ADAP deficiency in mice leads to microthrombocytopenia caused by a reduced platelet life span and ectopic (pro)platelet release. Lack of ADAP in MKs impairs demarcation membrane system polarization and podosome formation.

scholarly journals Enhancing functional platelet release in vivo from in vitro–grown megakaryocytes using small molecule inhibitors

2018 ◽  
Vol 2 (6) ◽  
pp. 597-606 ◽  
Author(s):  
Danuta Jarocha ◽  
Karen K. Vo ◽  
Randolph B. Lyde ◽  
Vincent Hayes ◽  
Rodney M. Camire ◽  
...  
Keyword(s):  
Small Molecule ◽  
Small Molecule Inhibitors ◽  
Key Points ◽  
Drug Treatments ◽  
Platelet Release

Key PointsDrugs shown to enhance megakaryocyte ploidy and size variably effect terminal injury and apoptosis of in vitro–grown megakaryocytes. The number of functional platelets released in vivo from infused megakaryocytes can be enhanced by these drug treatments.

scholarly journals FlnA binding to PACSIN2 F-BAR domain regulates membrane tubulation in megakaryocytes and platelets

Blood ◽  
2015 ◽  
Vol 126 (1) ◽  
pp. 80-88 ◽  
Author(s):  
Antonija Jurak Begonja ◽  
Fred G. Pluthero ◽  
Worawit Suphamungmee ◽  
Silvia Giannini ◽  
Hilary Christensen ◽  
...  
Keyword(s):  
Membrane System ◽  
Bar Domain ◽  
Key Points ◽  
Membrane Tubulation

Key PointsThe F-BAR protein PACSIN2 associates with the initiating demarcation membrane system in megakaryocytes. FlnA binding to the PACSIN2 F-BAR domain regulates membrane tubulation in megakaryocytes, platelets, and in vitro.

scholarly journals Deletion of the Arp2/3 complex in megakaryocytes leads to microthrombocytopenia in mice

2017 ◽  
Vol 1 (18) ◽  
pp. 1398-1408 ◽  
Author(s):  
David S. Paul ◽  
Caterina Casari ◽  
Congying Wu ◽  
Raymond Piatt ◽  
Swetha Pasala ◽  
...  
Keyword(s):  
Platelet Adhesion ◽  
Minor Role ◽  
Key Points ◽  
Platelet Release ◽  
A Minor ◽  
Lamellipodia Formation

Key Points Deletion of Arp2/3 leads to marked microthrombocytopenia due to abnormal platelet release and increased platelet clearance. Arp2/3 is critical for platelet lamellipodia formation and spreading, but plays a minor role for platelet adhesion and hemostasis.

scholarly journals IGF-1 facilitates thrombopoiesis primarily through Akt activation

Blood ◽  
2018 ◽  
Vol 132 (2) ◽  
pp. 210-222 ◽  
Author(s):  
Shilei Chen ◽  
Mengjia Hu ◽  
Mingqiang Shen ◽  
Song Wang ◽  
Cheng Wang ◽  
...  
Keyword(s):  
Akt Activation ◽  
Key Points ◽  
Platelet Release ◽  
Megakaryocyte Differentiation

Key Points IGF-1 has the ability to promote megakaryocyte differentiation, PPF, and platelet release. The effect of IGF-1 on thrombopoiesis is mediated primarily by AKT activation with the assistance of SRC-3.

Biogenesis of the demarcation membrane system (DMS) in megakaryocytes

Blood ◽  
2014 ◽  
Vol 123 (6) ◽  
pp. 921-930 ◽  
Author(s):  
Anita Eckly ◽  
Harry Heijnen ◽  
Fabien Pertuy ◽  
Willie Geerts ◽  
Fabienne Proamer ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Plasma Membrane ◽  
State Of The Art ◽  
Three Dimensional ◽  
Membrane System ◽  
Cleavage Furrow ◽  
Perinuclear Region ◽  
Dimensional Electron ◽  
Key Points

Key Points Using state-of-the-art three-dimensional electron microscopy approaches, we show that the onset of the DMS formation is at the megakaryocyte plasma membrane. A pre-DMS structure is formed in the perinuclear region, through a PM invagination process that resembles cleavage furrow formation.

scholarly journals Intrinsic apoptosis circumvents the functional decline of circulating platelets but does not cause the storage lesion

Blood ◽  
2018 ◽  
Vol 132 (2) ◽  
pp. 197-209 ◽  
Author(s):  
Irina Pleines ◽  
Marion Lebois ◽  
Pradnya Gangatirkar ◽  
Amanda E. Au ◽  
Rachael M. Lane ◽  
...  
Keyword(s):  
Life Span ◽  
Functional Decline ◽  
Intrinsic Apoptosis ◽  
Storage Lesion ◽  
Platelet Storage Lesion ◽  
Platelet Storage ◽  
Key Points

Key Points BAK/BAX depletion in murine platelets reveals that intrinsic apoptosis is not required for the development of the platelet storage lesion. Restriction of platelet life span by intrinsic apoptosis is pivotal to maintain a functional, hemostatically reactive platelet population.

scholarly journals Platelet bioreactor-on-a-chip

Blood ◽  
2014 ◽  
Vol 124 (12) ◽  
pp. 1857-1867 ◽  
Author(s):  
Jonathan N. Thon ◽  
Linas Mazutis ◽  
Stephen Wu ◽  
Joanna L. Sylman ◽  
Allen Ehrlicher ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Shear Stress ◽  
Blood Vessel ◽  
Key Points ◽  
Platelet Release

Key Points We have developed a biomimetic microfluidic platelet bioreactor that recapitulates bone marrow and blood vessel microenvironments. Application of shear stress in this bioreactor triggers physiological proplatelet production, and platelet release.

Closing the gap between T-cell life span estimates from stable isotope-labeling studies in mice and humans

Blood ◽  
2013 ◽  
Vol 122 (13) ◽  
pp. 2205-2212 ◽  
Author(s):  
Liset Westera ◽  
Julia Drylewicz ◽  
Ineke den Braber ◽  
Tendai Mugwagwa ◽  
Iris van der Maas ◽  
...  
Keyword(s):  
T Cell ◽  
Stable Isotope ◽  
Life Span ◽  
Isotope Labeling ◽  
Stable Isotope Labeling ◽  
Stable Isotope Label ◽  
Isotope Label ◽  
Cell Life ◽  
Key Points ◽  
Closing The Gap

Key Points Life span estimates can be sensitive to the duration of stable isotope label administration, explaining discrepancies in the literature. Multiexponential models are needed to obtain reliable leukocyte life span estimates.

A Freeze-Etch Study of Acinetobacter SP. Grown on a Hydrocarbon Substrate

1974 ◽  
Vol 32 ◽  
pp. 174-175
Author(s):  
C. L. Scott ◽  
W. R. Finnerty
Keyword(s):  
Membrane System ◽  
Structural Distortion ◽  
Intracytoplasmic Membrane ◽  
Gram Negative ◽  
Sectioned Material ◽  
Acinetobacter Sp

Acinetobacter sp. HO-1-N, a gram-negative hydrocarbon oxidizing bacterium previously designated Micrococcus cerificans, has been shown to sequester the hydrocarbon into intracytoplasmic pools as a result of growth on this substrate. In hydrocarbon grown cells, an intracytoplasmic membrane system was also observed along with a doubling of cellular phospholipids (Z). However, using conventional dehydration and embedding procedures in preparing thin sectioned material, the hydrocarbon is extracted from the cells. This may lead to structural distortion, consequently, the freeze-etch technique was applied to preserve the integrity of the cell.

A potassium permanganate fixative for membranes in sections

1990 ◽  
Vol 48 (3) ◽  
pp. 722-723
Author(s):  
Jindan Song
Keyword(s):  
Potassium Permanganate ◽  
Cultured Cells ◽  
Calf Serum ◽  
Trisodium Citrate ◽  
Membrane System ◽  
Adenocarcinoma Cell Line ◽  
Mouse Embryo Fibroblast ◽  
African Green Monkey Kidney ◽  
Adenocarcinoma Cell ◽  
Green Monkey

Potassium permanganate has been used as a fixative for the botanical specimen and membrane system in thin section by Glauert (1975). A new potassium permanganate fixative ( Trisodium citrate 60mM, Potassium chloride 25mM, Magnesium chloride 35mM, and Potassium permanganate 125mM ) for localizing membranous system in whole_mount cultured cells with standard trasmission electron microscopy and phase_contrast microscopy has been developed). Here, we report that using this new potassium permanganate fixative for membranous system in sections.Cultured cells, CV_1 (African green monkey kidney epithelial cells), Balb/c 3T3 ( Mouse embryo fibroblast ) and MCF_7 (Human adenocarcinoma cell line) were used for this study. All cells were grown on 35mm plastic dishes in DME medium containing 5% calf serum at 37 c with 100% humidity and 5% CO2. Using the potassium permanganate fixative to fix the cells for about 7 minutes. After fixation, the cells were dehydrated in a graded series of ethanol.

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