CD3-Zeta Gene Expression in Workers Benzene-Exposed and Benzene- Poisoned Workers

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 4925-4925
Author(s):  
Bo Li ◽  
Yangqiu Li ◽  
Shaohua Chen ◽  
Lijian Yang ◽  
Wei Yu ◽  
...  
Keyword(s):  
Gene Expression ◽  
T Cells ◽  
Mononuclear Cells ◽  
Skewed Distribution ◽  
Gene Expression Level ◽  
Expression Level ◽  
Peripheral Blood Mononuclear ◽  
Invariant Structure ◽  
Normal Individuals ◽  
Exposed Workers

Abstract Benzene is an industrial chemical and component of cigarette smoke, gasoline, and automobile emissions. Benzene’s toxic effects on the blood and bone marrow can induce aplastic anemia and leukemia. Benzene is known to be highly toxic to a variety of cell types including lymphocytes. Our previous study showed that skewed distribution and clonal expansion of TCR Vα subfamily T cells had been found in benzene-exposed workers, indicating that the disorder of T cell immune function might relate to benzene-exposed. The TCR expressed on the surface of T cells is associated with an invariant structure-CD3 and composed the TCR/CD3 complex. The CD3ζ chain plays an important role in the complex which involved in signal transduction. Little is known about the feature of CD3 ζ chain expression in benzene-exposed workers. To further identify the expression level of CD3 ζ gene in benzene-exposed workers, real-time PCR with SYBR Green±technique was used for detecting CD3 ζ gene expression level in peripheral blood mononuclear cells from 29 benzene-exposed workers, 42 benzene-poisoned workers and 20 normal individuals. β2- microglobulin gene (β2M) was used as an endogenous reference. Relative changes in CD3 ζ gene expression level was used by the 2−ΔCt×100% method (ΔCt=Ct(ζ) −Ct(β2M) ). CD3ζ gene could be detected in all of normal individuals, however, only 21 out of 29 benzeneexposed workers could be detected the CD3ζ gene with a mean expression level of 15.0 ±24.9, and in 33 of all 45 benzene-poisoned workers with mean value of 19.8 ±29.7. The detectable CD3 ζ gene expression level in both benzene-exposed and benzene-poisoned groups increased significantly compared with that in normal individuals (3.00±2.11, P< 0.05). This is, to our knowledge, the first description of the effect of benzene-exposed on the expression of the CD3 ζ gene. The abnormality expression of CD3 ζ gene might lead to immune dysfunction in benzene-exposed and benzene-poisoned workers. In addition, CD3ζ gene could not be detected in a part of samples, whether the absence of CD3ζ gene might related to dysfunction of T cells in workers with benzene-exposed and benzenepoisoned, it remains an open question.

The Feature of TCR Zeta Gene in CD4+ and CD8+ T Cells in Patients with CML.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 4526-4526
Author(s):  
Si Chen ◽  
Yangqiu Li ◽  
Shaohua Chen ◽  
Lijian Yang ◽  
Xiuli Wu ◽  
...  
Keyword(s):  
Gene Expression ◽  
T Cells ◽  
Cd8 T Cells ◽  
Cd4 T Cells ◽  
Gene Expression Level ◽  
Expression Level ◽  
Chain Gene ◽  
Zeta Chain ◽  
Normal Individuals ◽  
Normal Controls

Abstract The T-cell receptor (TCR) zeta chain is a master sensor and regulator of lymphocyte responses which plays a critical role in TCR-mediated signal transduction. The abnormal expression of TCR zeta gene was found in some malignancies and immune disease. Cellular immune deficiency is the common feature in patients with chronic myeloid leukemia (CML). Our previous studies had showed that 60% of CML patients displayed an abnormally low expression of TCR zeta chain in peripheral blood mononuclear cells (PBMCs). To further estimate the changes of zeta chain expression of T-lymphocyte subsets in CML, TCR zeta chain gene expression level in purified CD4+ and CD8+ T cells sorted by MACS were detected by real-Time PCR with SYBR Green I technique, CD4+ and CD8+ T cells from both 10 cases with CML and 10 normal individuals were selected to analyze in the study. β2 -microglobulin was used as an endogenous reference. Relative changes in TCR zeta gene expression level were used by the 2−ΔCt×100% method. The relative mRNA expression level of TCR zeta gene in CD4+ and CD8+ T cells from CML was 4.06±4.82% and 3.82±4.25% respectively, whereas 7.75±2.52% and 5.19±1.25% were found in normal individuals. There was a wide range in the TCR zeta gene expression level of CD4+ T cells from CML patients (0.23–15.93%,) with a median value of 1.77%, whereas the a range of (4.74–12.81%) with a median of 7.93% was showed in normal controls (P=0.046). The expression level of TCR zeta gene in CD8+ T cells from controls ranged from 3.47% to 6.75% (median 5.52%) and in CML patients from 0.86% to 15.55% with a median of 2.51% (P=0.340). The results indicated that, compared with normal controls, zeta chain gene expression was obviously down-regulated in CD4+ T cells from patients with CML. However, there was not significant different in expression level of TCR zeta gene of CD8+ T cells between CML patients and normal individuals. Moreover, the expression level of TCR zeta gene is nonsignificant age-associated or gender-link in CD4+ T cells and CD8+ T cells from patients with CML. In conclusions, the results provide at first the difference of TCR-zeta gene expression pattern in CD4+ and CD8+ T cells from patients with CML, this may be reflective of signaling difference between the different T cell subtypes in immunodeficiency state of patients.

scholarly journals Gene expression profile of human T cells following a single stimulation of peripheral blood mononuclear cells with anti-CD3 antibodies

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Isabel Garcia Sousa ◽  
Kelly Cristina Rodrigues Simi ◽  
Manuela Maragno do Almo ◽  
Maryani Andressa Gomes Bezerra ◽  
Gero Doose ◽  
...  
Keyword(s):  
Gene Expression ◽  
T Cells ◽  
Gene Expression Profile ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Human T Cells ◽  
Blood Mononuclear Cells ◽  
Stimulation Of

Expression Pattern of TCR-zeta Chain in Patients with Aplastic Anemia and Polycythemia Vera.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 3763-3763
Author(s):  
Lijian Yang ◽  
Yangqiu Li ◽  
Chuang Li ◽  
Shaohua Chen ◽  
Si Chen ◽  
...  
Keyword(s):  
Gene Expression ◽  
T Cells ◽  
Aplastic Anemia ◽  
Polycythemia Vera ◽  
Expression Pattern ◽  
Gene Expression Pattern ◽  
Gene Expression Level ◽  
Expression Level ◽  
Chain Gene ◽  
Zeta Chain

Abstract TCR zeta chain of TCR-CD3 receptor complex is though to be critical for T cells development and T cell effector function. The absence of TCR zeta chain not only influences the TCR expression on the cell membrane, but also impairs the proliferative response and the level of activation of mature T cells. Recently, the abnormal expression of TCR zeta chain was found different disease, for example in hematological malignancy. In the present study, we analyzed the expression level of TCR zeta chain gene in T cells from patients with aplastic anemia (AA) and polycythemia vera (PV), thereby to estimate the feature of T cells activation status. Real-time PCR with SYBR Green I technique was used for detecting the expression level of TCR zeta chain gene in peripheral blood mononuclear cells of patients with AA (25 cases),PV (12 cases), and 30 normal individuals. β2-microglobulin gene was used as an endogeneous reference. Evaluation of TCR zeta chain gene expression level were used the 2−ΔΔCt method. Compared with the normal control, TCR zeta chain gene expression level could be divided into two groups: 10 patients with AA (40%) and 5 patients with PV (41.7%) were over expression, while 15 patients with AA (60%) and 7 patients with PV (58.3%) were down expression. The expression level of TCR zeta gene is nonsignificat age-associated in both AA and PV patients. Moreover, the expression level of TCR zeta gene is nonsignificant age-associated or gender-link in T cells from patients with AA. The signaling pathway of T cells activation might display some disorder in patients with AA or PV. The effect of the change of TCR zeta chain gene expression pattern in AA and PV patients should be further confirmed.

Effect of β-D-Mannuronic Acid (M2000) on Oxidative Stress Enzymes’ Gene Using Healthy Donor Peripheral Blood Mononuclear Cells for Evaluating the Anti-Aging Property

2019 ◽  
Vol 16 (3) ◽  
pp. 265-271 ◽  
Author(s):  
Mahsa Taeb ◽  
Abdollah Jafarzadeh ◽  
Seyed Shahabeddin Mortazavi-Jahromi ◽  
Nahid Zainodini ◽  
Mohammad Reza Mirzaei ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Expression Level ◽  
High Dose ◽  
Peripheral Blood Mononuclear ◽  
Expression Levels ◽  
Blood Mononuclear Cells

Objective: This research aimed to study the anti-aging and anti-inflammatory effects of low and high doses of the β-D-mannuronic (M2000) on gene expression of enzymes involved in oxidative stress (including SOD2, GST, GPX1, CAT, iNOS, and MPO) in peripheral blood mononuclear cells (PBMCs) of healthy donors under in vitro conditions. Methods: The PBMCs were separated and the RNAs were then extracted and the cDNAs synthesized, and expression levels of the mentioned genes were detected by qRT-PCR. Results: Our results indicated that the high dose of this drug could significantly reduce the expression level of the SOD2 gene compared to the lipopolysaccharide (LPS) group (p < 0.0001). Moreover, it was found that the high dose of this drug could significantly decrease the expression level of the GST gene compared to the LPS group (p < 0.0001). However, no significant reductions were observed in expression levels of the CAT and GPX1 genes compared to the LPS group. Furthermore, our data revealed that the level of iNOS and MPO gene expression was significantly reduced, in both doses of M2000, respectively, compared to the LPS group (p < 0.0001). Conclusion: This research showed that M2000 as a novel NSAID with immunosuppressive properties could modify oxidative stress through lowering expression levels of the SOD2, GST, iNOS, and MPO genes compared to the healthy expression levels, with a probable reduction of the risk of developing inflammatory diseases related to age and aging.

Association of changes in T regulatory cells (Treg) during nivolumab treatment with melanoma outcome.

2017 ◽  
Vol 35 (15_suppl) ◽  
pp. 3031-3031 ◽  
Author(s):  
Jeffrey S. Weber ◽  
Rupal Ramakrishnan ◽  
Andressa Laino ◽  
Anders E. Berglund ◽  
David Woods
Keyword(s):  
Gene Expression ◽  
T Cells ◽  
Cd4 T Cells ◽  
T Regulatory Cells ◽  
Mononuclear Cells ◽  
In Vitro Assays ◽  
Peripheral Blood Mononuclear ◽  
Suppressive Function ◽  
Blocking Antibodies

3031 Background: PD-1 blocking antibodies have significant efficacy in the treatment of melanoma; however, many patients fail to respond and resistance mechanisms remain unknown. We addressed the role of Tregs, an immunosuppressive T-cell population, in patient outcome after treatment with nivolumab. Methods: Peripheral blood mononuclear cells (PBMC) were obtained from patients on trials with nivolumab as adjuvant therapy for resected disease or as treatment for metastatic melanoma. To measure suppression, Tregs were flow-sorted from PBMC and evaluated in allogeneic mixed lymphocyte reactions. Tregs and conventional CD4 T-cells were evaluated for gene expression changes by RNA-sequencing. Treg percentages and phosphorylated STAT3 (pSTAT3) expression were evaluated by flow cytometry. The effects of PD-1 blockade with nivolumab were evaluated in vitro using T-cells from baseline patient PBMC samples. Results: Tregs from responding patients or adjuvant patients without evidence of disease (NED) had reduced suppressive function post-nivolumab (p < 0.05), but no changes were observed in relapsing/non-responding patients; their Tregs were more suppressive than NED/responding Tregs (p < 0.001). NED Tregs had unique gene expression changes and associated pathways post-nivolumab compared to relapsing patient Tregs and conventional CD4 T-cells, including up-regulation of proliferation pathways (q < 8e-19) and downregulation of oxidative phosphorylation (q < 7e-5). NED Tregs had upregulation of pSTAT3 expression post-nivolumab (p < 0.05), which was not observed in relapsing patients. Evaluation of Tregs from patients with active disease also showed upregulation of pSTAT3 in responders (p < 0.05) but not non-responders. The relative increase in Treg pSTAT3 was associated with increased overall survival (R2= 0.49, p < 0.05). In vitro assays using PD-1 blocking antibodies recapitulated the increase in pSTAT3 (p < 0.05) and Treg percentages (p < 0.001), which were diminished with the addition of a STAT3 inhibitor (p < 0.01). Conclusions: These results demonstrate previously unknown roles of decreased Treg suppressive function and induction of STAT3 as biomarkers of patient’s outcome to nivolumab therapy.

Detection of sjTRECs within Peripheral Blood T Cells Evaluation of Recent Thymic Output Function in Myelodysplastic Syndrome.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 4919-4919
Author(s):  
Xin Du ◽  
Yang-qiu Li ◽  
Jian-yu Weng ◽  
Ze-sheng Lu ◽  
Rong Guo ◽  
...  
Keyword(s):  
T Cells ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Output Function ◽  
Peripheral Blood Mononuclear ◽  
Thymic Output ◽  
Normal Individuals ◽  
Blood Mononuclear Cells ◽  
Immunological Abnormalities

Abstract Objective The myelodysplastic syndromes (MDS) comprise a heterogeneous group of clonal hematopoietic stem cell disorders, while, immunological abnormalities are frequently observed in patients with MDS. Several reports revealed that about 10% of MDS patients have clinical autoimmune disorders like skin vasculitis, rheumatic disease, or autoimmune hemolytic anemia. Furthermore, serological immunological abnormalities like hyper- or hypogammaglobulinemia, positivities of antinuclear antibody, positivities of direct Coombs test, or inverted CD4/8 ratios were found in 18–65% of patients with MDS. Recently immunosuppressive therapies including prednisolone, antithymocyte globulin, and cyclosporin A (CsA) are used to treat cytopenia in some patients with MDS. But it isn’t very clear the immunosuppressive mechanism in MDS and the value of the treatment. To analyze the content of signal joint Tcell receptor excision DNA circles signal joint T cell receptor excision DNA circles(sjTRECs) within peripheral blood mononuclear cells (PBMCs), thereby to infer the level of naive T cells and the recent thymic output function in patients with myelodyspoastic syndrom. Methods Quantitative detection of sjTRECs in DNA of peripheral blood mononuclear cells from 13 normal individuals and 8 patienets were performed by real-time polymerase chain reaction (PCR) and TaqMan technique. Results The median value of sjTRECs copies P1 000 PBMCs was 4.37±3.64 in normal individuals whereas it was1.07 ±1.40 copies P1 000 PBMCs in myelodysplastic syndrom patients (P &lt;0. 05). Conclusions MDS Patients decrease in recent thymic output function.

The Feature of TCR Vβ Repertoire, Thymic Recent Output Function and TCR-zeta Chain Expression in Patients with Immune Thrombocytopenic Purpura.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 2099-2099
Author(s):  
Xueli Zhang ◽  
Yangqiu Li ◽  
Shaohua Chen ◽  
Lijian Yang ◽  
Si Chen ◽  
...  
Keyword(s):  
Gene Expression ◽  
T Cells ◽  
T Cell ◽  
Immune Thrombocytopenic Purpura ◽  
Clonal Expansion ◽  
Expression Level ◽  
Chain Gene ◽  
Output Function ◽  
Thrombocytopenic Purpura ◽  
Normal Individuals

Abstract Chronic idiopathic/immune thrombocytopenic purpura (ITP) is an autoimmune disorder in which antiplatelet antibodies induce destruction of platelets. T cells may play a critical role in controlling the synthesis of autoantibodies, and may relate to initiation of ITP. In order to identify the feature of T cells immune state in patients with ITP, TCR Vβ repertoire which can identify T cell proliferation in response to antigenic stimulation; signal joint T-cell receptor excision DNA circles (TRECs) which can use to estimate the thymic recent naïve T cells output function and the expression level of TCR ζ chain gene which defined as an important factor in T cells signaling. Specific Vβ family primers, RT-PCR and genescan technique were use to analyze the expression of TCR Vβ subfamily and the clonality of TCR Vβ T cells in cDNA form peripheral blood mononuclear cells (PBMCs) of 5 ITP patients; quantitative analysis of TRECs in DNA of PBMCs from 15 cases were preformed by real-time PCR (TaqMan); real-time PCR with SYBR Green I technique was used for detecting TCR ζ gene expression level in cDNA of PBMCs from of 18 patients, β2 -microglobulin gene was used as an endogenous reference, relative changes in TCR ζ chain gene expression level were used by the 2− ΔCt method. 25 normal individuals served as control. The results showed that only 4–11 Vβ subfamily T cells could be identified in ITP cases. The frequent expression Vβ subfamilies were Vβ2 (100%), Vβ3 (100%), Vβ19 (80%) and Vβ21 (80%), while it could not detected the expression of Vβ4, Vβ6–12, Vβ17, Vβ20 and Vβ24. However, all 24 Vβ subfamilies could be detected in samples from normal individuals and all of them displayed polyclonality, clonal expansion Vβ T cells could be found in some subfamilies in every patient. The frequent clonal expansion T cells were Vβ21 subfamily which could be identified in 4 out of 5 cases. The mean value of TRECs was 2.60±2.99 copies/1000 PBMCs in ITP patients, it was not significantly difference (p&gt;0.05) in comparison with normal group (3.76±3.42 copies/1000 PBMCs), although the TRECs level displayed changefully in different patients with ITP. In four out of fifteen ITP cases no TRECs copies could be detected in more than 10000 PBMCs. The higher frequency of TRECs was found in female group, however it was not statistical significance in comparison with male group. The expression of TCR ζ chain gene could be detected in all of 25 normal samples, and TCR ζ gene was found in 17 out of 18 cases with ITP. However, the expression level of TCR ζ gene was lower in ITP samples than those from normal individuals (P=0.017). The expression level of TCR ζ gene is nonsignificat age-associated or gender-link in ITP patients. In conclusions, the restricted expression of TCR Vb subfamilies and clonal expanded Vβ T cells are the T-cell immune feature in ITP, and the frequent presentation of clonal expanded Vβ21 T cells may relate to the disorder of cellular immune function in ITP. At least, in most cases with ITP, the normal level of naïve T cells and thymic recent output function were detected. It is, to our knowledge, the first description in the expression feature of TCR ζ gene in ITP patients, which displayed down expression, it might be a feature in autoimmune disease.

A time dependent gene expression level of C3, C3aR and CTSL in CD4+ T cells

Immunobiology ◽  
2016 ◽  
Vol 221 (10) ◽  
pp. 1201
Author(s):  
Cecilie Bo Hansen ◽  
Anton Willer ◽  
Hanne Vibeke Hansen Marquart ◽  
Martin Kolev ◽  
Claudia Kemper ◽  
...  
Keyword(s):  
Gene Expression ◽  
T Cells ◽  
Cd4 T Cells ◽  
Time Dependent ◽  
Gene Expression Level ◽  
Expression Level

Analysis of the T-Cell Receptor Vα Gene Repertoire and Clonal Expansion in the Benzene-Exposed Group.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 3874-3874
Author(s):  
Bo Li ◽  
Yangqiu Li ◽  
Shaohua Chen ◽  
Lijian Yang ◽  
Jiayu Chen ◽  
...  
Keyword(s):  
T Cells ◽  
Mononuclear Cells ◽  
Clonal Expansion ◽  
Cell Receptor ◽  
Exposed Group ◽  
Skewed Distribution ◽  
Gene Repertoire ◽  
Peripheral Blood Mononuclear ◽  
Pcr Products ◽  
Expression Rate

Abstract Benzene is a potent human leukemogen, but its mechanism of hematotoxicity is uncertain. It is well know that benzene inhibit T cells proliferation. Several reports revealed that clonal expansion TCR Vβ T cells could be found in workers exposed to benzene. In this study we observe the distribution of TCR Vα gene repertoire and clonal expansion in peripheral blood mononuclear cells from 9 donors and 16 workers exposed to benzene. Complementarity determining region 3 (CDR3) of TCR Vα subfamily genes were amplified using RT-PCR. The PCR products were further analyzed by genescan to evaluating clonality of T cells. 29 Vα subfamily could be detected in 9 donors.1~11 Vα subfamilies were identified in all but one of the workers studied. The most frequently expressed Vα subfamily were Vα3, Vα12 and Vα19 (68.8%), Vα14(56.3%), with a lower expression rate found in Vα5,Vα15, Vα16, Vα 22, Vα 23 and Vα 24 (6.3%). Clonal expansion T cells in one or more Vα subfamily were found in 12 out of all workers studied, including oligoclonal, oligocolonal trend and bioclonal patterns. The frequency of clonal expansion T cells in Vα12, Vα14 and Vα19 subfamilies were higher than others. In conclusion, skewed distribution and clonal expansion of TCR Vα subfamily T cells could be found in workers exposed to benzene. Vα12, Vα14 and Vα19 subfamilies may be highly sensitive to benzene exposed. This is the first report of clonal expansion TCR Vα T cells in the benzene-exposed group. The bias pattern of TCR Vα T cells may be due to the immune cytotocicity from benzene. However, whether the oligoclonality in some Vα subfamilies reflect the phenomenon of clone absense or may be a response clone to benzene-related impairment during exposed to benzene, remains an open question.

The Feature of TCR Vγ And TCR Vδ Repertoire Distribution and Clonality in Patients with Immune Thrombocytopeinc Purpura.

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 3409-3409
Author(s):  
Xueli Zhang ◽  
Shaohua Chen ◽  
Lijian Yang ◽  
Kanger Zhu ◽  
Yangqiu Li
Keyword(s):  
T Cells ◽  
Mononuclear Cells ◽  
Γδ T Cells ◽  
Autoimmune Disorder ◽  
Expression Level ◽  
Peripheral Blood Mononuclear ◽  
Platelet Antibodies ◽  
Immune Mediated ◽  
Healthy Control ◽  
Oligoclonal Expansion

Abstract Chronic idiopathic/immune thrombocytopenic purpura (ITP) is an autoimmune disorder in which anti-platelet antibodies induce platelets destruction due to an imbalanced immune response. Recently, data indicated the γδ+T cells may play an important role in mediated autoimmune disease. Our previous study has showed the restricted expression of TCR Vβ subfamilies and the alteration of peripheral TCR Vβ repertoire pattern in the majority of ITP patients. In the present study, we farther analyze the feature of TCR Vγ and TCR Vδ repertoire distribution and clonality in patients with ITP. The CDR3 size of three TCR Vγ and eight TCR Vδ subfamily genes were analyzed in peripheral blood mononuclear cells (PBMCs) from 8 cases with ITP and 10 healthy individuals, using RT-PCR and genescan technique. To determine the expression level of TCR Vγ subfamily genes, quantitative analysis of TCR Vγ I–III subfamilies was performed by real-time PCR. TCR Vγ I to III subfamilies could be detected in the most samples from ITP as well as in healthy control. However, oligoclonal expansion of TCR Vγ I was identified in 3 cases with ITP, which display polyclonality in all of samples from healthy control. The expression level of all TCR Vγ I–III subfamilies in PBMCs from ITP was significant lower than that from healthy control (P=0.003,P=0.000,P=0.005 respectively). the pattern of TCR Vγ I–III repertoire in ITP was TCR VγI> TCR VγIII> TCR VγII, in contrast, TCR VγII> TCR VγI> TCR VγII was found in healthy control. TCR Vδ1 and TCR Vδ2 could be detected in most samples from ITP as well as in healthy control, whereas TCR Vδ3 could be detected only in 2 out of 8 cases with ITP, which could be found in 90% of healthy controls (p=0.02), TCR Vδ8 could not be identified in all of samples from ITP, which could be found in 30% of healthy control. Oligoclonal expanded TCR Vδ1and TCR Vδ2 T cells could be identified in the half of samples from ITP, similar results were found in healthy control. In conclusions, the alteration of peripheral TCR Vγ and TCR Vδ repertoire pattern might be important in the pathogenesis of immune-mediated platelet destruction in some cases with ITP. Our report is the first description of feature of TCR Vγ and TCR Vδ repertoire pattern in ITP patients.

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