scholarly journals Cell size and plamitate-1-14c turnover of rabbit marrow fat

Blood ◽  
1977 ◽  
Vol 49 (4) ◽  
pp. 599-605 ◽  
Author(s):  
S Trubowitz ◽  
A Bathija
Keyword(s):  
Fatty Acids ◽  
Cell Volume ◽  
Unsaturated Fatty Acids ◽  
Fat Cell ◽  
Marrow Fat ◽  
Mean Cell Volume ◽  
Triglyceride Fraction ◽  
Mean Diameter ◽  
The Mean ◽  
Perinephric Fat

Abstract Adipose tissue is a major component of normal rabbit marrow. Morphological considerations suggest an active role for this tissue in hematopoiesis. This hypothesis was tested by injecting 50 micronCi of palmitate-1–14C intravenously into fed, hematologically normal New Zealand rabbits. The animals were sacrificed 24 hr later and the femoral marrow removed. Samples of subcutaneous and perinephric fat were taken for comparison. The fat cells were isolated by the Rodbell method and the diameters measured. Incorporation of the 14C-palmitate in the triglyceride fraction was determined and the composition of the fatty acids was measured by gas chromatography. The mean diameter of the marrow fat cell was 46 micronm (mean cell volume 55 pl); the mean diameter of the perinephric fat cell 70 micronm (mean cell volume 200 pl). 14C-Palmitate turnover per gram triglyceride was some fivefold greater in the marrow fat; however, when expressed on a cell basis, the turnover for the marrow and perinephric fat cell was similar. The marrow fat contained a higher concentration of unsaturated fatty acids. These findings suggest that there is greater lipolysis and lesser storage in the marrow fat than in the perinephric.

Cell size and plamitate-1-14c turnover of rabbit marrow fat

Blood ◽  
1977 ◽  
Vol 49 (4) ◽  
pp. 599-605 ◽  
Author(s):  
S Trubowitz ◽  
A Bathija
Keyword(s):  
Fatty Acids ◽  
Cell Volume ◽  
Unsaturated Fatty Acids ◽  
Fat Cell ◽  
Marrow Fat ◽  
Mean Cell Volume ◽  
Triglyceride Fraction ◽  
Mean Diameter ◽  
The Mean ◽  
Perinephric Fat

Adipose tissue is a major component of normal rabbit marrow. Morphological considerations suggest an active role for this tissue in hematopoiesis. This hypothesis was tested by injecting 50 micronCi of palmitate-1–14C intravenously into fed, hematologically normal New Zealand rabbits. The animals were sacrificed 24 hr later and the femoral marrow removed. Samples of subcutaneous and perinephric fat were taken for comparison. The fat cells were isolated by the Rodbell method and the diameters measured. Incorporation of the 14C-palmitate in the triglyceride fraction was determined and the composition of the fatty acids was measured by gas chromatography. The mean diameter of the marrow fat cell was 46 micronm (mean cell volume 55 pl); the mean diameter of the perinephric fat cell 70 micronm (mean cell volume 200 pl). 14C-Palmitate turnover per gram triglyceride was some fivefold greater in the marrow fat; however, when expressed on a cell basis, the turnover for the marrow and perinephric fat cell was similar. The marrow fat contained a higher concentration of unsaturated fatty acids. These findings suggest that there is greater lipolysis and lesser storage in the marrow fat than in the perinephric.

Alteration of the erythrocyte ultrastructure and blood viscosity by morphine

1978 ◽  
Vol 56 (2) ◽  
pp. 245-251 ◽  
Author(s):  
E. B. Vadas ◽  
E. A. Hosein
Keyword(s):  
Cell Volume ◽  
Blood Viscosity ◽  
Whole Blood ◽  
Plasma Viscosity ◽  
Cell Geometry ◽  
Flow Properties ◽  
Mean Cell Volume ◽  
Morphine Administration ◽  
The Mean ◽  
Volume Cell

The effects of acute morphine administration on intact erythrocytes and on their flow properties were studied by measuring the mean cell volume, cell geometry, and whole blood and plasma viscosities. Morphine caused a small (2–7%) increase in mean cell volume. Changes in cell geometry were found to be time dependent and most pronounced in concave portions of the red cells. Whole blood viscosity was found to decrease upon morphine treatment; this may be due in part to a concurrent decrease in plasma viscosity.

scholarly journals Antimicrobial Activity of Red Fruit (Pandanus conoideus Lam) Paste against Staphylococcus aureus and Escherichia coli in Burger Patties

2021 ◽  
Vol 16 (2) ◽  
pp. 132-143
Author(s):  
Selvia Tharukliling ◽  
Lilik Eka Radiati ◽  
Imam Thohari ◽  
Agus Susilo
Keyword(s):  
Fatty Acids ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Unsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Ethanol Extract ◽  
Plate Count ◽  
Significant Difference ◽  
The Mean ◽  
Paste Content

This study aims to determine the antimicrobial activity of red fruit paste against Staphylococcus aureus FNCC-0047 and Eschericia coli FNCC-0091 by using ethanol and n-hexane as well as the total plate count value of the patty with red fruit paste added on different observations day. The concentration of paste extract for antimicrobial activity test was 6.25%, 12.5%, 25%, 50%. The calculation of the total value of the burger patty plate is calculated on the 3rd, 7th and 14th d with the red fruit paste content in the formula as much as 0%, 5%, 10%, 15%. The results showed that the fatty acids of red fruit paste were dominated by oleic acid and linoleic acid, which are unsaturated fatty acids and palmitic acid, which are saturated fatty acids. The yield of red fruit paste in n-hexane solvent was higher than ethanol solvent. There was a significant difference (P <0.05) from the use of different solvents to the mean clear zone of the two bacteria tested. The antibacterial activity shown by the ethanol extract and n-hexane extract of red fruit paste was in the inactive category at the 6.12% level, the moderate category at the 12.5% to 25% level and the strong category at the 50% level. There was a significant difference (P <0.05) from the total microbial value in each treatment where the higher the red fruit paste content in the patty, the lower the total microbial value found on each d of observation. The use of red fruit paste at a level of 10% to 15% can withstand the rate of microbial growth

scholarly journals The mean cell volume difference (dMCV) reflects serum hypertonicity in diabetic dogs

PLoS ONE ◽  
2019 ◽  
Vol 14 (7) ◽  
pp. e0219864 ◽  
Author(s):  
Olga C. Norris ◽  
Thomas Schermerhorn
Keyword(s):  
Cell Volume ◽  
Mean Cell Volume ◽  
The Mean ◽  
Diabetic Dogs ◽  
Volume Difference

scholarly journals cholesterol content and fatty acid composition of most consumed turkish hard and soft cheeses

2011 ◽  
Vol 23 (No. 4) ◽  
pp. 166-172 ◽  
Author(s):  
O. Kinik ◽  
O. Gursoy ◽  
A.K. Seckin
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Unsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Cholesterol Content ◽  
Oleic Acid Content ◽  
Soft Cheese ◽  
The Mean

Cholesterol content and fatty acid composition of 29 different most popular hard (Tulum, Teneke Tulum, aged Kashar, and fresh Kashar cheeses) and soft cheese (White Pickled cheeses) samples from the markets ofIzmirinTurkeywere determined by gas chromatography. Cholesterol content of hard and soft cheeses ranged from 46.47 to 138.99 mg/100&nbsp;g fat. Relative to the mean cholesterol values, the highest cholesterol content was found in fresh Kashar cheese. The fatty acid composition is quite similar in all samples. As concerns the saturated fatty acids, the most abundant in the cheeses investigated were palmitic (C16:0), stearic (C18:0), and myristic acids (C14:0). Palmitic acid levels were found to be the highest of the saturated fatty acid in all samples. Oleic acid content (5.93&ndash;29.38 mg/100 g fatty acids) in all cheeses was considerable higher than those of other unsaturated fatty acids. No specific trend or correlation between cholesterol and individual fatty acids was observed. &nbsp;

scholarly journals Reference and cut-off values for serum ferritin, mean cell volume, and hemoglobin to diagnose iron deficiency in infants aged 9 to 12 months

Medicina ◽  
2007 ◽  
Vol 43 (9) ◽  
pp. 698 ◽  
Author(s):  
Neve Vendt ◽  
Tiina Talvik ◽  
Pille Kool ◽  
Sirje Leedo ◽  
Karel Tomberg ◽  
...  
Keyword(s):  
Iron Deficiency ◽  
Cell Volume ◽  
Serum Ferritin ◽  
Reference Values ◽  
Mean Cell Volume ◽  
Receiver Operating Characteristic Curves ◽  
Healthy Infants ◽  
Diagnostic Characteristics ◽  
The Mean ◽  
Better Than

Background. The number of different laboratory tests and reference values are used to diagnose iron deficiency, but there is no agreement regarding the diagnostic criteria for infants. Aim of study. To establish reference values for serum ferritin, mean cell volume, and hemoglobin in infants aged from 9 to 12 months in Estonia and to evaluate the diagnostic characteristics of serum ferritin, mean cell volume, and hemoglobin in the diagnosis of iron deficiency. Methods. Altogether 195 healthy infants aged 9–12 months participated in the study. They were randomly selected out of 300 families from seven different counties from all over Estonia. Serum ferritin, hemoglobin, soluble transferrin receptor (sTfR) levels and mean cell volume were measured. The best cut-off values for serum ferritin, mean cell volume, and hemoglobin to diagnose iron deficiency, defined by sTfR>2.45 mg/L (n=25), were determined by receiver operating characteristic curves. Results. The mean and reference values (5th and 95th centiles) for ferritin was 24 µg/L (4– 55), 73 fl (68–80) for mean cell volume, and 112 g/L (101–128) for hemoglobin. The best cut-off values to diagnose iron deficiency were <10.9 µg/L for serum ferritin (sensitivity of 83% and specificity of 80%), <71 fl for mean cell volume (86% and 83%, respectively), and <107 g/L for hemoglobin (67% and 87%, respectively). The sensitivity and specificity of serum ferritin and mean cell volume in the diagnosis of iron deficiency were better than those of hemoglobin. Conclusion. For the diagnosis of iron deficiency in infants aged 9–12 months, the cut-off values of <10.9 µg/L and <71 fl should be used for serum ferritin and mean cell volume, respectively.

An assessment of the incidence of iron deficiency in paediatric otolaryngology inpatients

1991 ◽  
Vol 105 (12) ◽  
pp. 1021-1024 ◽  
Author(s):  
Judith M. Heaton ◽  
Robin L. Blair ◽  
Clemency Shadbolt ◽  
Helen Christmas
Keyword(s):  
Regression Analysis ◽  
Iron Deficiency ◽  
Haemoglobin Level ◽  
Cell Volume ◽  
Surgical Procedures ◽  
Ferritin Level ◽  
Preoperative Screening ◽  
Mean Cell Volume ◽  
Economic Class ◽  
The Mean

AbstractThe aims of this study were: to determine whether there is an increased incidence of iron deficiency in paediatric otolaryngology inpatients compared with other surgical controls; and to establish whether preoperative screening of haemoglobin level is warranted in such patients.Children aged 1–10 years admitted electively for ENT surgery or for general surgical procedures had blood taken for haemoglobin level, mean cell volume and serum ferritin. Their age, weight, socio-economic class and ethnic background were recorded.A total of 100 patients entered the study, in a six-month period. The mean ages and weights for the two groups were statistically different, so allowance was made for this in calculations. Social class was not significantly different. No relationship could be established between haemoglobin level and ferritin level for individual patients. Multiple regression analysis for haemoglobin level, mean cell volume and for ferritin level showed that allowing for the age and weight differences these variables were not significantly different for the two groups.This study has therefore shown no increased incidence of iron deficiency in paediatric ENT inpatients. Each Department should formulate its own policy on pre-operative haemoglobin screening, based on local considerations.

Effects of Chloramphenicol on Growth, Size Distribution, Chlorophyll Synthesis and Ultrastructure of Euglena Gracilis

1969 ◽  
Vol 4 (3) ◽  
pp. 627-644
Author(s):  
Y. BEN-SHAUL ◽  
Y. MARKUS
Keyword(s):  
Protein Synthesis ◽  
Cell Division ◽  
Cell Volume ◽  
Chlorophyll Synthesis ◽  
Secondary Effect ◽  
Mean Cell Volume ◽  
Initial Rise ◽  
Dividing Cells ◽  
Light Inhibition ◽  
The Mean

Multiplication of Euglena cells treated by 0.5-1.0 mg/ml chloramphenicol was not disturbed for the first 36 h and inhibition appeared only at later stages. The mean cell volume of treated dividing cells was decreased, although the initial rise in cell volume, which normally occurred during the first 12 h of incubation, was not prevented. The antibiotic also lowered the chlorophyll content of green dividing cells. In dard-grown cells transferred to light, inhibition of chlorophyll synthesis was immediate but not complete, and was followed by a decreased rate of plastid elongation and thylakoid formation. Our findings suggest that chloramphenicol does not cause the loss of existing pigment and that impaired chlorophyll synthesis is a secondary effect of inhibition of protein synthesis. The results also indicate that the greening process is more sensitive than cell division to the antibiotic.

scholarly journals Some hematological parameters of Wistar rats treated with Chromolaena odorata leave extracts

2017 ◽  
Vol 90 (1) ◽  
Author(s):  
Henshaw Uchechi Okoroiwu ◽  
Ifeyinwa Maryann Okafor ◽  
Emmanuel Kufre Uko ◽  
Item Justin Atangwho
Keyword(s):  
Cell Volume ◽  
Wistar Rats ◽  
Hematological Parameters ◽  
Ethanol Extract ◽  
Control Group ◽  
Chromolaena Odorata ◽  
Mean Cell Volume ◽  
Significant Difference ◽  
The Mean ◽  
Ethanol Extracts

This study was designed to investigate the effects of the different extracts of Chromolaena odorata leave on the hematopoietic system of Wistar rats. Solvent extraction was used for the ethanol and aqueous extractions while decoction method was used for the crude extraction. Fifty Wistar rats of both sexes weighing 140-180 g were used for this study. They were divided into ten groups each containing five rats. The animals were fed the extracts by oral gavage once daily for 21 days. Blood sample was collected via cardiac artery. Hematological parameters were analyzed using automation method. The ethanol extract gave the highest extract yield. The aqueous, ethanol and crude extraction had median lethal toxicity (LD50) of 2738.6 mg/kg, 1581.1 mg/kg and 224.7 mg/kg, respectively. Significant difference (P<0.05) in the total white blood cell count was observed in the 75 mg/kg ethanol and 300 mg/kg crude extracts when compared with control group. Significant difference (P<0.05) in the hemoglobin concentration was observed in the 150 mg/kg ethanol extracts when compared with the control group. Significant difference (P<0.05) in the packed cell volume was seen in the 75 mg/kg aqueous, 150 mg/kg aqueous and 75 mg/kg ethanol extracts in respect to the control group. The mean cell volume, the mean platelet volume and platelet large cell ratio of the 75 mg/kg aqueous extract were significantly different (P<0.05) when compared with the control group. The present study showed possible treatment-induced hematopoietic function of C. odorata leave extracts.

The influence of anaesthetics on the haematology of the patas monkey, Erythrocebus patas

1978 ◽  
Vol 12 (3) ◽  
pp. 167-170 ◽  
Author(s):  
C. M. Hawkey ◽  
S. Dean ◽  
M. G. Hart
Keyword(s):  
Cell Count ◽  
Cell Volume ◽  
Fibrinolytic Activity ◽  
White Cell Count ◽  
Erythrocebus Patas ◽  
Suitable Alternative ◽  
Mean Cell Volume ◽  
Patas Monkey ◽  
Significant Difference ◽  
The Mean

The effects of phencyclidine, ketamine and an alphaxalone-alphadolone mixture on the haematology of the patas monkey have been compared. In animals sedated with phencyclidine or ketamine the only significant difference detected was in the mean cell volume. Statistically significant differences in white-cell count and blood coagulation and fibrinolytic activity were found in monkeys which had received alphaxalone-alphadolone. It is suggested that ketamine is a suitable alternative to phencyclidine for haematological studies in these monkeys.

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