Proliferation kinetics of Sezary cells

Abstract The proliferation kinetics of neoplastic T cells arising in Sezary syndrome (Sezary cells) are still poorly understood. Kinetic studies with 3H-thymidine as a DNA precursor revealed a low incorporation rate of the nucleotide into Sezary cells obtained from the peripheral blood versus Sezary cells from the skin. Using the double-label autoradiography we determined the duration of single cell cycle phases of blood and cutaneous Sezary cells. The results indicate the almost complete lack of proliferative activity in the blood but a considerable portion of proliferatively active Sezary cells in skin infiltrates. The removal of a large mass of quiescent blood cells by leukapheresis did not affect the proliferative state of the residual peripheral cell population implying that the procedure did not induce the migration of proliferating skin cells towards the blood. Terminally, the disease underwent transition into immunoblastic lymphoma. At this time, the kinetic behavior of the peripheral immunoblasts showed great similarity to that of cutaneous Sezary cells. The findings point towards a common extravascular production site of Sezary cells and immunoblasts probably located in the lymphatic tissue.

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Proliferation kinetics of Sezary cells

Blood ◽

10.1182/blood.v57.6.1049.bloodjournal5761049 ◽

1981 ◽

Vol 57 (6) ◽

pp. 1049-1054

Author(s):

JD Schwarzmeier ◽

E Paietta ◽

T Radaszkiewicz ◽

K Konrad ◽

L Marosi

Keyword(s):

Kinetic Studies ◽

Large Mass ◽

Lymphatic Tissue ◽

Peripheral Cell ◽

Proliferation Kinetics ◽

Skin Cells ◽

Considerable Portion ◽

Double Label ◽

Kinetics Of ◽

Sézary Cells

The proliferation kinetics of neoplastic T cells arising in Sezary syndrome (Sezary cells) are still poorly understood. Kinetic studies with 3H-thymidine as a DNA precursor revealed a low incorporation rate of the nucleotide into Sezary cells obtained from the peripheral blood versus Sezary cells from the skin. Using the double-label autoradiography we determined the duration of single cell cycle phases of blood and cutaneous Sezary cells. The results indicate the almost complete lack of proliferative activity in the blood but a considerable portion of proliferatively active Sezary cells in skin infiltrates. The removal of a large mass of quiescent blood cells by leukapheresis did not affect the proliferative state of the residual peripheral cell population implying that the procedure did not induce the migration of proliferating skin cells towards the blood. Terminally, the disease underwent transition into immunoblastic lymphoma. At this time, the kinetic behavior of the peripheral immunoblasts showed great similarity to that of cutaneous Sezary cells. The findings point towards a common extravascular production site of Sezary cells and immunoblasts probably located in the lymphatic tissue.

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Kinetic Studies of Cs+ and Sr2+ Ion Exchange Using Clinoptilolite in Static Columns and an Agitated Tubular Reactor (ATR)

ChemEngineering ◽

10.3390/chemengineering5010009 ◽

2021 ◽

Vol 5 (1) ◽

pp. 9

Author(s):

Muhammad Yusuf Prajitno ◽

Mohamad Taufiqurrakhman ◽

David Harbottle ◽

Timothy N. Hunter

Keyword(s):

Adsorption Capacity ◽

Tubular Reactor ◽

Process Intensification ◽

Kinetic Studies ◽

Breakthrough Curves ◽

Maximum Adsorption Capacity ◽

Batch Studies ◽

Shear Mixing ◽

Natural Clinoptilolite ◽

Kinetics Of

Natural clinoptilolite was studied to assess its performance in removing caesium and strontium ions, using both static columns and an agitated tube reactor (ATR) for process intensification. Kinetic breakthrough curves were fitted using the Thomas and Modified Dose Response (MDR) models. In the static columns, the clinoptilolite adsorption capacity (qe) for 200 ppm ion concentrations was found to be ~171 and 16 mg/g for caesium and strontium, respectively, highlighting the poor material ability to exchange strontium. Reducing the concentration of strontium to 100 ppm, however, led to a higher strontium qe of ~48 mg/g (close to the maximum adsorption capacity). Conversely, halving the column residence time to 15 min decreased the qe for 100 ppm strontium solutions to 13–14 mg/g. All the kinetic breakthrough data correlated well with the maximum adsorption capacities found in previous batch studies, where, in particular, the influence of concentration on the slow uptake kinetics of strontium was evidenced. For the ATR studies, two column lengths were investigated (of 25 and 34 cm) with the clinoptilolite embedded directly into the agitator bar. The 34 cm-length system significantly outperformed the static vertical columns, where the adsorption capacity and breakthrough time were enhanced by ~30%, which was assumed to be due to the heightened kinetics from shear mixing. Critically, the increase in performance was achieved with a relative process flow rate over twice that of the static columns.

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Biochar Chemistry in a Weathered Tropical Soil: Kinetics of Phosphorus Sorption

Agriculture ◽

10.3390/agriculture11040295 ◽

2021 ◽

Vol 11 (4) ◽

pp. 295

Author(s):

Marina Moura Morales ◽

Nicholas Brian Comerford ◽

Maurel Behling ◽

Daniel Carneiro de Abreu ◽

Iraê Amaral Guerrini

Keyword(s):

Mineral Soil ◽

Soil Surface ◽

Kinetic Studies ◽

Inorganic Phosphorus ◽

Initial Reaction ◽

Phosphorus Sorption ◽

P Sorption ◽

Published Research ◽

Kinetics Of

The phosphorus (P) chemistry of biochar (BC)-amended soils is poorly understood. This statement is based on the lack of published research attempting a comprehensive characterization of biochar’s influence on P sorption. Therefore, this study addressed the kinetic limitations of these processes. This was accomplished using a fast pyrolysis biochar made from a mix of waste materials applied to a highly weathered Latossolo Vermelho distrofico (Oxisol) from São Paulo, Brazil. Standard method (batch method) was used. The sorption kinetic studies indicated that P sorption in both cases, soil (S) and soil-biochar (SBC), had a relatively fast initial reaction between 0 to 5 min. This may have happened because adding biochar to the soil decreased P sorption capacity compared to the mineral soil alone. Presumably, this is a result of: (i) Inorganic phosphorus desorbed from biochar was resorbed onto the mineral soil; (ii) charcoal particles physically covered P sorption locations on soil; or (iii) the pH increased when BC was added SBC and the soil surface became more negatively charged, thus increasing anion repulsion and decreasing P sorption.

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Effects of radiation on the cell proliferation kinetics of epithelial tissues—therapeutic implications

British Journal of Radiology ◽

10.1259/0007-1285-69-825-795 ◽

1996 ◽

Vol 69 (825) ◽

pp. 795-803 ◽

Cited By ~ 17

Author(s):

G M Morris

Keyword(s):

Cell Proliferation ◽

Proliferation Kinetics ◽

Therapeutic Implications ◽

Epithelial Tissues ◽

Effects Of Radiation ◽

Kinetics Of

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INHOMOGENEOUS LARGE DEFORMATION KINETICS OF POLYMERIC GELS

International Journal of Applied Mechanics ◽

10.1142/s1758825113500014 ◽

2013 ◽

Vol 05 (01) ◽

pp. 1350001 ◽

Cited By ~ 45

Author(s):

WILLIAM TOH ◽

ZISHUN LIU ◽

TENG YONG NG ◽

WEI HONG

Keyword(s):

Large Deformation ◽

Soft Matter ◽

Inner Core ◽

Kinetic Studies ◽

Fe Model ◽

One Dimensional ◽

Simplified Approach ◽

Deformation Kinetics ◽

Polymeric Gels ◽

Kinetics Of

This work examines the dynamics of nonlinear large deformation of polymeric gels, and the kinetics of gel deformation is carried out through the coupling of existing hyperelastic theory for gels with kinetic laws for diffusion of small molecules. As finite element (FE) models for the transient swelling process is not available in commercial FE software, we develop a customized FE model/methodology which can be used to simulate the transient swelling process of hydrogels. The method is based on the similarity between diffusion and heat transfer laws by determining the equivalent thermal properties for gel kinetics. Several numerical examples are investigated to explore the capabilities of the present FE model, namely: a cube to study free swelling; one-dimensional constrained swelling; a rectangular block fixed to a rigid substrate to study swelling under external constraints; and a thin annulus fixed at the inner core to study buckling phenomena. The simulation results for the constrained block and one-dimensional constrained swelling are compared with available experimental data, and these comparisons show a good degree of similarity. In addition to this work providing a valuable tool to researchers for the study of gel kinetic deformation in the various applications of soft matter, we also hope to inspire works to adopt this simplified approach, in particular to kinetic studies of diffusion-driven mechanisms.

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The Proliferation Kinetics of Pulmonary Alveolar Macrophages

Journal of Leukocyte Biology ◽

10.1002/jlb.35.3.317 ◽

1984 ◽

Vol 35 (3) ◽

pp. 317-327 ◽

Cited By ~ 39

Author(s):

J.E. Coggle ◽

J.D. Tarling

Keyword(s):

Alveolar Macrophages ◽

Proliferation Kinetics ◽

Pulmonary Alveolar Macrophages ◽

Kinetics Of

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Kinetics of K release from soils of Brazilian coffee regions: effect of organic acids

Revista Brasileira de Ciência do Solo ◽

10.1590/s0100-06832008000200008 ◽

2008 ◽

Vol 32 (2) ◽

pp. 533-540 ◽

Cited By ~ 9

Author(s):

Vladimir Antônio Silva ◽

Giuliano Marchi ◽

Luiz Roberto Guimarães Guilherme ◽

José Maria de Lima ◽

Francisco Dias Nogueira ◽

...

Keyword(s):

Release Kinetics ◽

Kinetic Studies ◽

Release Rates ◽

First Order ◽

Potassium Release ◽

Minas Gerais State ◽

Soil Clay ◽

Extractant Solution ◽

Kinetics Of ◽

K Release

Kinetic studies on soil potassium release can contribute to a better understanding of K availability to plants. This study was conducted to evaluate K release rates from the whole soil, clay, silt, and sand fractions of B-horizon samples of a basalt-derived Oxisol and a sienite-derived Ultisol, both representative soils from coffee regions of Minas Gerais State, Brazil. Potassium was extracted from each fraction after eight different shaking time periods (0-665 h) with either 0.001 mol L-1 citrate or oxalate at a 1:10 solid:solution ratio. First-order, Elovich, zero-order, and parabolic diffusion equations were used to parameterize the time dependence of K release. For the Oxisol, the first-order equation fitted best to the experimental data of K release, with similar rates for all fractions and independent of the presence of citrate or oxalate in the extractant solution. For all studied Ultisol fractions, in which K release rates increased when extractions were performed with citrate solution, the Elovich model described K release kinetics most adequately. The highest potassium release rate of the Ultisol silt fraction was probably due to the transference of "non-exchangeable" K to the extractant solution, whereas in the Oxisol exchangeable potassium represented the main K source in all studied fractions.

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The chemical and kinetic consequences of the modification of papain by N-bromosuccinimide

Canadian Journal of Biochemistry ◽

10.1139/o77-059 ◽

1977 ◽

Vol 55 (4) ◽

pp. 424-432

Author(s):

Bernard R. Glick ◽

Lewis J. Brubacher

Keyword(s):

Kinetic Studies ◽

Peptide Inhibitors ◽

Tryptophan Residue ◽

Difference Spectroscopy ◽

Tyrosine Residues ◽

Residue Determination ◽

Chain Cleavage ◽

Modified Enzyme ◽

Kinetics Of ◽

Twofold Decrease

Nonactivated papain was treated with N-bromosuccinimide at pH 4.75. The N-bromosuccinimide-modified enzyme was characterized by (1) the change in absorbance at 280 nm, (2) amino acid analysis, (3) separate chemical determinations of tryptophan and tyrosine, (4) difference spectroscopy, and (5) an N-terminal residue determination. It is concluded that N-bromosuccinimide in sevenfold molar excess oxidizes one tryptophan and two to three tyrosine residues per molecule of nonactivated papain, without causing peptide chain cleavage. Kinetic studies with several substrates and competitive peptide inhibitors were performed at pH 6 using the N-bromosuccinimide-modified papain. In addition, the kinetics of the modified enzyme with the substrate α-N-benzoyl-L-arginine ethyl ester were studied in the region of pH 3.5–9.0. All substrates (and inhibitors) tested, with the exception of α-N-benzoyl-L-arginine p-nitroanilide, displayed approximately a twofold decrease in both kcat and Km (or Ki), relative to the native enzyme. It is concluded that the key tryptophan residue which is modified is probably Trp-177.

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