scholarly journals Extent of intravital contraction of arterial and venous thrombi and pulmonary emboli

2021 ◽  
Author(s):  
Rafael R. Khismatullin ◽  
Shahnoza Abdullayeva ◽  
Alina D. Peshkova ◽  
Khetam Sounbuli ◽  
Natalia G Evtugina ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Blood Cells ◽  
Ex Vivo ◽  
The Body ◽  
Pulmonary Emboli ◽  
Structural Rearrangements ◽  
Exponential Relationship ◽  
Blood Clots ◽  
Scanning Electron

Blood clots and thrombi undergo platelet-driven contraction/retraction followed by structural rearrangements. We have established quantitative relationships between the composition of blood clots and extent of contraction to determine intravital contraction of thrombi and emboli based on their content. The composition of human blood clots and thrombi was quantified using histology and scanning electron microscopy. Contracting blood clots segregated into the gradually shrinking outer layer that contains a fibrin-platelet mesh and the expanding inner portion with compacted red blood cells (RBCs). At 10% contraction, biconcave RBCs were partially compressed into polyhedral RBCs, which became dominant at 20% contraction and higher. The polyhedral/biconcave RBC ratio and the extent of contraction displayed an exponential relationship, which was used to determine the extent of intravital contraction of ex vivo thrombi, ranging from 30% to 50%. In venous thrombi, the extent of contraction decreased gradually from the older (head) to the younger (body, tail) parts. In pulmonary emboli, the extent of contraction was significantly lower than in the venous head, but was similar to the body and tail, suggesting that the emboli originate from the younger portion(s) of venous thrombi. The extent of contraction in arterial cerebral thrombi was significantly higher than in the younger parts of venous thrombi (body, tail) and pulmonary emboli, but was indistinguishable from the older part (head). A novel tool, named the "contraction ruler," has been developed to use the composition of ex vivo thrombi to assess the extent of their intravital contraction, which contributes to the pathophysiology of thromboembolism.

Scanning electron microscopy of erythrophagocytosis by Entamoeba histolytica trophozoites

1992 ◽  
Vol 50 (1) ◽  
pp. 880-881
Author(s):  
Victor Tsutsumi ◽  
Adolfo Martinez-Palomo ◽  
Kyuichi Tanikawa
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Red Blood Cells ◽  
Entamoeba Histolytica ◽  
Causative Agent ◽  
Blood Cells ◽  
Protozoan Parasite ◽  
Complex Phenomenon ◽  
Great Capacity ◽  
Scanning Electron

The protozoan parasite Entamoeba histolytica is the causative agent of amebiasis in man. The trophozoite or motile form is a highly dynamic and pleomorphic cell with a great capacity to destroy tissues. Moreover, the parasite has the singular ability to phagocytize a variety of different live or death cells. Phagocytosis of red blood cells by E. histolytica trophozoites is a complex phenomenon related with amebic pathogenicity and nutrition.

THE STUDY OF RED BLOOD CELLS BY SCANNING ELECTRON MICROSCOPY

2016 ◽  
Vol 26 (3) ◽  
pp. 381-390 ◽  
Author(s):  
Sargylana N. Mamayeva ◽  
◽  
Yana A. Munkhalova ◽  
Irina V. Kononova ◽  
Afanasiy A. Dyakonov ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Scanning Electron

SCANNING ELECTRON MICROSCOPE: POSSIBILITIES FOR STUDYING KIDNEY DISEASES

2020 ◽  
pp. 176-182
Author(s):  
Mamaeva S.N. ◽  
Vinokurov R.R. ◽  
Munkhalova Ya.A. ◽  
Dyakonova D.P. ◽  
Platonova V.A. ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Filtration Rate ◽  
Kidney Diseases ◽  
First Time ◽  
Scanning Electron

Currently, due to the intensive development of high-tech science-intensive medical and research devices, more and more attention is paid to the development of diagnostics of rare and difficult to diagnose diseases. It is known that among numerous nephropathies, hematuria may be the only symptom of kidney and urinary tract diseases, which complicates their diagnosis and treatment. In order to develop new approaches for the diagnosis of nephropathies, the authors have been studying the morphology of red blood cells in the blood and urine of children and adults using a scanning electron microscope for several years. The paper presents the results of studies of children with various kidney diseases, including IgA-nephropathy, and chronic glomerulonephritis. Scanning electron microscopy was used for the first time to detect nanoparticles on the surface of red blood cells, the size of which is comparable to the size of viruses, which became the basis for one of the authors ' assumptions, namely, the possible transport of certain types of viruses by red blood cells. Thus, some kidney diseases could be considered virus-associated. This paper presents for the first time the results of determining the glomerular filtration rate of both kidneys separately in the study of separate kidney function and of the study of urine smears obtained during catheterization of the ureters in patients with hydronephrosis of one of the kidneys by scanning electron microscopy. As in previous studies, nanoparticles were found on the surface of red blood cells, which leads to the conclusion about the possible viral nature of the disease of the considered patient. In addition, smear images obtained using a microscope showed a significant difference in the elements of the right and left kidneys urine, which did not contradict the data on the study of glomerular filtration rate. According to the authors, the capabilities of the scanning electron microscope can be applied in fundamental research of kidney diseases at the cellular and molecular levels, forming new ideas about their origin, as well as on the basis of which new methods of non-invasive diagnostics can be built.

Preparation of Alcohol-Preserved Larvae of Culicidae (Diptera) for Scanning Electron Microscopy

1972 ◽  
Vol 3 (3) ◽  
pp. 181-188 ◽  
Author(s):  
Christine Dahl
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
The Body ◽  
Taxonomic Studies ◽  
Scanning Electron ◽  
Sem Studies

AbstractA method for preparation of alcohol-preserved culicid larvae for Scanning Electron Microscope (SEM) studies is described. It is based on dehydration by ethanol-xylol and fast evaporation of xylol in +8o° C. for ten minutes. For taxonomic studies such as examination of pecten teeth, comb scales and microtrichiae in magnifications up to 6oooX the method is suitable. For studies of receptor structures on hair-tufts and microstructures of the body integument alcohol preserved material is less satisfactory. The microstructure of the comb scales is figured and their function discussed. Differences in the ultrastructure of the abdominal hair-tufts are pointed out.

scholarly journals Congenital infection with Setaria digitata and Setaria marshalli in the thoracic cavity of a Korean calf: a case report

2010 ◽  
Vol 55 (No. 6) ◽  
pp. 275-280 ◽  
Author(s):  
NS Kim ◽  
HC Kim ◽  
C. Sim ◽  
JR Ji ◽  
NS Kim ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Case Report ◽  
Congenital Infection ◽  
The Body ◽  
Thoracic Cavity ◽  
Setaria Digitata ◽  
Scanning Electron

In March 2010, a 3.5-month-old Korean native calf was anatomized and two nematode worms were detected in the thoracic cavity. The worms were identified and classified by light and scanning electron microscopy on the basis of features at the anterior and posterior parts of the worms. The worms were female Setaria digitata and Setaria marshalli and numerous eggs which contained microfilaria were detected in the uterus of both species. The body lengths of the S. digitata and S. marshalli were 78 mm and 117 mm, respectively. Mosquitoes act as the vector for Setaria nematodes but these are inactivated in winter in Korea. Therefore, we concluded that this case represented setariasis with congenital infection occurring during the summer prenatal stage of life.

scholarly journals Balantidium grimi n. sp. (Ciliophora, Litostomatea), a new species inhabiting the rectum of the frog Quasipaa spinosa from Lishui, China

Parasite ◽  
2018 ◽  
Vol 25 ◽  
pp. 29 ◽  
Author(s):  
Weishan Zhao ◽  
Can Li ◽  
Dong Zhang ◽  
Runqiu Wang ◽  
Yingzhen Zheng ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Phylogenetic Analysis ◽  
New Species ◽  
Body Shape ◽  
Ssu Rdna ◽  
The Body ◽  
Molecular Phylogenetic ◽  
A New Species ◽  
Scanning Electron

Balantidium grimi n. sp. is described from the rectum of the frog Quasipaa spinosa (Amphibia, Dicroglossidae) from Lishui, Zhejiang Province, China. The new species is described by both light microscopy (LM) and scanning electron microscopy (SEM), and a molecular phylogenetic analysis is also presented. This species has unique morphological features in that the body shape is somewhat flattened and the vestibulum is “V”-shaped, occupying nearly 3/8 to 4/7 of the body length. Only one contractile vacuole, situated at the posterior body, was observed. The phylogenetic analysis based on SSU-rDNA indicates that B. grimi groups together with B. duodeni and B. entozoon. In addition, the genus Balantidium is clearly polyphyletic.

Composition and Microstructure of a Natural Nano-Structure Mineral: Six-Ring Rock

2011 ◽  
Vol 295-297 ◽  
pp. 869-872
Author(s):  
Qing Shan Li ◽  
Xin Wang ◽  
Jun Liu ◽  
Guang Zhong Xing
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Energy Dispersive Spectrometer ◽  
The Body ◽  
X Ray Diffraction ◽  
Nano Structure ◽  
X Ray ◽  
Transmission Electron ◽  
Nano Size ◽  
Scanning Electron

Six-ring Rock is widely used as containers of water and additives to produce health care products. In this paper, the composition and microstructure of Six-ring Rock have been investigated by using scanning electron microscopy, energy dispersive spectrometer, transmission electron microscopy, x-ray diffraction and other technologies. Results show that Six-ring Rock is composed of CaMg(CO3)2, SiO2 and KAlSi3O8. Fe atoms exist in CaMg(CO3)2 by replacing Mg atoms. Six-ring Rock shows nano-size lamellar and acerose microstructures on the surface, and nano-size monocrystals in the body. Six-ring Rock is a natural nano structure mineral.

Scanning electron microscopy of the cuticular armature of the nematode Gnathostoma spinigerum Owen, 1836 from cats in Laos

1990 ◽  
Vol 64 (3) ◽  
pp. 255-262 ◽  
Author(s):  
Tomáš Scholz ◽  
Oleg Ditrich
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Spatial Arrangement ◽  
Felis Catus ◽  
The Body ◽  
Immature Female ◽  
Gnathostoma Spinigerum ◽  
First Time ◽  
Scanning Electron

ABSTRACTThe body surface of an immature female Gnathostoma spinigerum found for the first time in the definitive host (Felis catus f. domestica) in Laos was studied using a scanning electron microscope. All types of cuticular spines, which are one of the most important features for species identification of gnathostomid nematodes, together with their spatial arrangement, are described and figured.

scholarly journals Splendid coloration of the peacock spider Maratus splendens

2016 ◽  
Vol 13 (121) ◽  
pp. 20160437 ◽  
Author(s):  
Doekele G. Stavenga ◽  
Jürgen C. Otto ◽  
Bodo D. Wilts
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Optical Properties ◽  
Air Gap ◽  
The Body ◽  
Structural Coloration ◽  
Optical Modelling ◽  
Jumping Spiders ◽  
Blue Coloration ◽  
Scanning Electron

Jumping spiders are well known for their acute vision and often bright colours. The male peacock spider Maratus splendens is richly coloured by scales that cover the body. The colours of the white, cream and red scales, which have an elaborate shape with numerous spines, are pigmentary. Blue scales are unpigmented and have a structural colour, created by an intricate photonic system consisting of two chitinous layers with ridges, separated by an air gap, with on the inner sides of the chitin layers an array of filaments. We have characterized the optical properties of the scales by microspectrophotometry, imaging scatterometry and light and scanning electron microscopy. Optical modelling revealed that the filament array constitutes a novel structural coloration system, which subtly fine tunes the scale reflectance to the observed blue coloration.

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