scholarly journals Development and clinical translation of tubular constructs for tracheal tissue engineering: a review

2021 ◽  
Vol 30 (162) ◽  
pp. 210154
Author(s):  
Luis Soriano ◽  
Tehreem Khalid ◽  
Derek Whelan ◽  
Niall O'Huallachain ◽  
Karen C. Redmond ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Congenital Abnormalities ◽  
Lessons Learned ◽  
Short Length ◽  
Clinical Translation ◽  
Tracheal Resection ◽  
Big Picture ◽  
Tracheal Tissue ◽  
Tracheal Replacement ◽  
Trachealis Muscle

Effective restoration of extensive tracheal damage arising from cancer, stenosis, infection or congenital abnormalities remains an unmet clinical need in respiratory medicine. The trachea is a 10–11 cm long fibrocartilaginous tube of the lower respiratory tract, with 16–20 tracheal cartilages anterolaterally and a dynamic trachealis muscle posteriorly. Tracheal resection is commonly offered to patients suffering from short-length tracheal defects, but replacement is required when the trauma exceeds 50% of total length of the trachea in adults and 30% in children. Recently, tissue engineering (TE) has shown promise to fabricate biocompatible tissue-engineered tracheal implants for tracheal replacement and regeneration. However, its widespread use is hampered by inadequate re-epithelialisation, poor mechanical properties, insufficient revascularisation and unsatisfactory durability, leading to little success in the clinical use of tissue-engineered tracheal implants to date. Here, we describe in detail the historical attempts and the lessons learned for tracheal TE approaches by contextualising the clinical needs and essential requirements for a functional tracheal graft. TE manufacturing approaches explored to date and the clinical translation of both TE and non-TE strategies for tracheal regeneration are summarised to fully understand the big picture of tracheal TE and its impact on clinical treatment of extensive tracheal defects.

Dexamethasone loaded bilayered 3D tubular scaffold reduces restenosis at the anastomotic site of tracheal replacement: in vitro and in vivo assessments

Nanoscale ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 4846-4858 ◽  
Author(s):  
Sang Jin Lee ◽  
Ji Suk Choi ◽  
Min Rye Eom ◽  
Ha Hyeon Jo ◽  
Il Keun Kwon ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Patient Specific ◽  
Anastomotic Site ◽  
Tubular Scaffold ◽  
Recent Developments ◽  
Tracheal Tissue ◽  
The Creation ◽  
Tracheal Replacement

Despite recent developments in the tracheal tissue engineering field, the creation of a patient specific substitute possessing both appropriate mechanical and biointerfacial properties remains challenging.

scholarly journals Scaffold-free tracheal cartilage engineering using roller bottle culture

2021 ◽  
Author(s):  
Imran Sheikh
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Articular Chondrocytes ◽  
Primary Anastomosis ◽  
Roller Bottle ◽  
Cartilage Engineering ◽  
Bovine Articular Chondrocytes ◽  
Tracheal Tissue ◽  
Tracheal Replacement

Resection with primary anastomosis can only repair up to 50% of the adult trachea and up to 30% of the pediatric trachea when damaged. There is a strong clinical need for long-segment tracheal replacements. The goal of this research was to create a seamless, scaffold-free cartilage cylinder for tracheal tissue engineering in vitro. Primary bovine articular chondrocytes were seeded onto tracheal moulds for roller bottle culture and the effect of rotational speed, growth factor supplementation, and chondrocyte layering were investigated. After the 4-week culture period, samples were evaluated biochemically, histologically, and biomechanically. The results indicated that rotation was necessary for full tissue coverage, with slower rotational speeds generating thicker tissue with an improved extracellular matrix, IGF-1 supplementation generating thicker tissue rich in glycosaminoglycans with inferior mechanical properties, and chondrocyte layering producing thinner tissue with increased mechanical properties. Overall, scaffold-free tissue engineering can generate seamless cylindrical cartilage constructs using roller bottle culture for future applications in long-segment tracheal replacement.

scholarly journals Scaffold-free tracheal cartilage engineering using roller bottle culture

2021 ◽  
Author(s):  
Imran Sheikh
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Articular Chondrocytes ◽  
Primary Anastomosis ◽  
Roller Bottle ◽  
Cartilage Engineering ◽  
Bovine Articular Chondrocytes ◽  
Tracheal Tissue ◽  
Tracheal Replacement

Resection with primary anastomosis can only repair up to 50% of the adult trachea and up to 30% of the pediatric trachea when damaged. There is a strong clinical need for long-segment tracheal replacements. The goal of this research was to create a seamless, scaffold-free cartilage cylinder for tracheal tissue engineering in vitro. Primary bovine articular chondrocytes were seeded onto tracheal moulds for roller bottle culture and the effect of rotational speed, growth factor supplementation, and chondrocyte layering were investigated. After the 4-week culture period, samples were evaluated biochemically, histologically, and biomechanically. The results indicated that rotation was necessary for full tissue coverage, with slower rotational speeds generating thicker tissue with an improved extracellular matrix, IGF-1 supplementation generating thicker tissue rich in glycosaminoglycans with inferior mechanical properties, and chondrocyte layering producing thinner tissue with increased mechanical properties. Overall, scaffold-free tissue engineering can generate seamless cylindrical cartilage constructs using roller bottle culture for future applications in long-segment tracheal replacement.

3D bioprinting for lung and tracheal tissue engineering: Criteria, advances, challenges, and future directions

Bioprinting ◽  
2021 ◽  
Vol 21 ◽  
pp. e00124
Author(s):  
Seyed Hossein Mahfouzi ◽  
Seyed Hamid Safiabadi Tali ◽  
Ghassem Amoabediny
Keyword(s):  
Tissue Engineering ◽  
3D Bioprinting ◽  
Future Directions ◽  
Tracheal Tissue

scholarly journals Current Strategies for Tracheal Replacement: A Review

Life ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 618
Author(s):  
Giuseppe Damiano ◽  
Vincenzo Davide Palumbo ◽  
Salvatore Fazzotta ◽  
Francesco Curione ◽  
Giulia Lo Monte ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Neck Region ◽  
Vascular Supply ◽  
Dimensional Structure ◽  
Tracheal Resection ◽  
Biological Scaffolds ◽  
Biological Substrate ◽  
Immunological Reactions ◽  
Tracheal Replacement ◽  
Current Lines

Airway cancers have been increasing in recent years. Tracheal resection is commonly performed during surgery and is burdened from post-operative complications severely affecting quality of life. Tracheal resection is usually carried out in primary tracheal tumors or other neoplasms of the neck region. Regenerative medicine for tracheal replacement using bio-prosthesis is under current research. In recent years, attempts were made to replace and transplant human cadaver trachea. An effective vascular supply is fundamental for a successful tracheal transplantation. The use of biological scaffolds derived from decellularized tissues has the advantage of a three-dimensional structure based on the native extracellular matrix promoting the perfusion, vascularization, and differentiation of the seeded cell typologies. By appropriately modulating some experimental parameters, it is possible to change the characteristics of the surface. The obtained membranes could theoretically be affixed to a decellularized tissue, but, in practice, it needs to ensure adhesion to the biological substrate and/or glue adhesion with biocompatible glues. It is also known that many of the biocompatible glues can be toxic or poorly tolerated and induce inflammatory phenomena or rejection. In tissue and organ transplants, decellularized tissues must not produce adverse immunological reactions and lead to rejection phenomena; at the same time, the transplant tissue must retain the mechanical properties of the original tissue. This review describes the attempts so far developed and the current lines of research in the field of tracheal replacement.

Fabrication and characterization of customized tubular scaffolds for tracheal tissue engineering by using solvent based 3D printing on predefined template

2021 ◽  
Vol 27 (2) ◽  
pp. 421-428
Author(s):  
Rudranarayan Kandi ◽  
Pulak Mohan Pandey ◽  
Misba Majood ◽  
Sujata Mohanty
Keyword(s):  
Tissue Engineering ◽  
Cell Adhesion ◽  
3D Printing ◽  
Chemical Properties ◽  
Contact Angle Measurement ◽  
Angle Measurement ◽  
In Vitro Cytotoxicity ◽  
Content Type ◽  
Tracheal Tissue

Purpose This paper aims to discuss the successful fabrication of customized tubular scaffolds for tracheal tissue engineering with a novel route using solvent-based extrusion 3D printing. Design/methodology/approach The manufacturing approach involved extrusion of polymeric ink over a rotating predefined pattern to construct customized tubular structure of polycaprolactone (PCL) and polyurethane (PU). Dimensional deviation in thickness of scaffolds were calculated for various layer thicknesses of 3D printing. Physical and chemical properties of scaffolds were investigated by scanning electron microscope (SEM), contact angle measurement, Fourier Transform Infrared Spectroscopy (FTIR) and X-ray diffraction (XRD). Mechanical characterizations were performed, and the results were compared to the reported properties of human native trachea from previous reports. Additionally, in vitro cytotoxicity of the fabricated scaffolds was studied in terms of cell proliferation, cell adhesion and hemagglutination assay. Findings The developed fabrication route was flexible and accurate by printing customized tubular scaffolds of various scales. Physiochemical results showed good miscibility of PCL/PU blend, and decrease in crystalline nature of blend with the addition of PU. Preliminary mechanical assessments illustrated comparable mechanical properties with the native human trachea. Longitudinal compression test reported outstanding strength and flexibility to maintain an unobstructed lumen, necessary for the patency. Furthermore, the scaffolds were found to be biocompatible to promote cell adhesion and proliferation from the in vitro cytotoxicity results. Practical implications The attempt can potentially meet the demand for flexible tubular scaffolds that ease the concerns such as availability of suitable organ donors. Originality/value 3D printing over accurate predefined templates to fabricate customized grafts gives novelty to the present method. Various customized scaffolds were compared with conventional cylindrical scaffold in terms of flexibility.

Tracheal tissue-engineering: in-vivo biocompatibility of mechanically-stripped allogenic rabbit trachea with autologous epithelial covering

2016 ◽  
Vol 116 (3) ◽  
pp. 164-174 ◽  
Author(s):  
Margot Den Hondt ◽  
Bart M. Vanaudenaerde ◽  
Erik K. Verbeken ◽  
Jan J. Vranckx
Keyword(s):  
Tissue Engineering ◽  
Tracheal Tissue

Vascularization strategies for tissue engineering for tracheal reconstruction

2021 ◽  
Author(s):  
Fei Sun ◽  
Yi Lu ◽  
Zhihao Wang ◽  
Hongcan Shi
Keyword(s):  
Tissue Engineering ◽  
Cell Metabolism ◽  
Microvascular Network ◽  
Related Factors ◽  
Engineering Technology ◽  
Term Survival ◽  
Tracheal Reconstruction ◽  
Tracheal Replacement ◽  
Therapy Strategies

Tissue engineering technology provides effective alternative treatments for tracheal reconstruction. The formation of a functional microvascular network is essential to support cell metabolism and ensure the long-term survival of grafts. Although several tracheal replacement therapy strategies have been developed in the past, the critical significance of the formation of microvascular networks in 3D scaffolds has not attracted sufficient attention. Here, we review key technologies and related factors of microvascular network construction in tissue-engineered trachea and explore optimized preparation processes of vascularized functional tissues for clinical applications.

Organic- or Inorganic-based Nanomaterials: Opportunities and Challenges in the Selection for Biomedicine

2021 ◽  
Vol 27 ◽  
Author(s):  
Ranjith Kankala
Keyword(s):  
Chemical Composition ◽  
Physicochemical Properties ◽  
Surface Area ◽  
Therapeutic Strategies ◽  
Lessons Learned ◽  
Clinical Translation ◽  
Significant Progress ◽  
Morphological Attributes ◽  
The Past ◽  
Selection For

: Since the inception of nanotechnology, several efforts have been dedicated to fabricating diverse nanodevices with exceptional performance. These innovative constructs have been applied in medicine due to their tailorable physicochemical properties (chemical composition, optical activity, spectra, and charge) and morphological attributes (size, shape, and surface area). Moreover, these versatile nanomedicines could promisingly offer better performance over the conventional therapeutic strategies. Broadly speaking, in terms of chemical composition, nanobiomaterials are classified into two predominant categories of organic and inorganic-based components. Despite their success and enormous versatile advancements in the past two decades, the significant progress towards clinical translation has been hampered by their corresponding intrinsic limitations. In this perspective, we give a brief overview of these organic- and inorganic-based materials, highlighting opportunities and challenges towards their utilization in medicine. Finally, we provide an interesting outlook in lessons learned and looking forward to developing these materials, emphasizing their scope towards clinical translation.

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