scholarly journals Analysis of Gene expression in soybean (Glycine max) roots in response to the root knot nematode Meloidogyne incognita using microarrays and KEGG pathways

BMC Genomics ◽  
2011 ◽  
Vol 12 (1) ◽  
Author(s):  
Heba MM Ibrahim ◽  
Parsa Hosseini ◽  
Nadim W Alkharouf ◽  
Ebtissam HA Hussein ◽  
Abd El Kader Y Gamal El-Din ◽  
...  
Keyword(s):  
Gene Expression ◽  
Glycine Max ◽  
Meloidogyne Incognita ◽  
Root Knot Nematode ◽  
Kegg Pathways

scholarly journals Genome expression dynamics reveals parasitism regulatory landscape of the root-knot nematode Meloidogyne incognita and a promoter motif associated with effector genes

2021 ◽  
Author(s):  
Martine Da Rocha ◽  
Caroline Bournaud ◽  
Julie Dazeniere ◽  
Peter Thorpe ◽  
Clement Pellegrin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Meloidogyne Incognita ◽  
Life Stage ◽  
Regulation Of Gene Expression ◽  
Root Knot Nematode ◽  
Root Knot Nematodes ◽  
Control Of Gene Expression ◽  
Precise Control ◽  
Effector Genes ◽  
Spatio Temporal

Root-knot nematodes are the major contributor to the crop losses caused by nematodes. Root-knot nematodes secrete effectors into the plant, derived from two sets of pharyngeal gland cells, to manipulate host physiology and immunity. Successful completion of the life cycle, involving successive molts from egg to adult, covers morphologically and functionally distinct stages and will require precise control of gene expression, including effectors. The details of how root-knot nematodes regulate transcription remain sparse. Here, we report a life stage-specific transcriptome of Meloidogyne incognita. Combined with an available annotated genome, we explore the spatio-temporal regulation of gene expression. We reveal gene expression clusters and predicted functions that accompany the major developmental transitions. Focusing on effectors, we identify a putative cis-regulatory motif associated with expression in the dorsal glands: providing an insight into effector regulation. We combine the presence of this motif with several other criteria to predict a novel set of putative dorsal gland effectors. Finally, we show this motif, and thereby its utility, is broadly conserved across the Meloidogyne genus and termed it Mel-DOG. Taken together, we provide the first genome-wide analysis of spatio-temporal gene expression in a root-knot nematode, and identify a new set of candidate effector genes that will guide future functional analyses.

Single pass cDNA sequencing - a powerful tool to analyse gene expression in preparasitic juveniles of the southern root-knot nematode Meloidogyne incognita

Nematology ◽  
2001 ◽  
Vol 3 (2) ◽  
pp. 129-139 ◽  
Author(s):  
Jaap Bakker ◽  
Fred Gommers ◽  
Geert Smant ◽  
Pierre Abad ◽  
Marie-Noëlle Rosso ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cdna Library ◽  
Meloidogyne Incognita ◽  
Sequence Data ◽  
Homo Sapiens ◽  
Large Fraction ◽  
Nematode Species ◽  
Cdna Libraries ◽  
Parasitic Nematodes ◽  
Root Knot Nematode

AbstractExpressed sequence tags (EST) have been widely used to assist in gene discovery in various organisms (e.g., Arabidopsis thaliana, Caenorhabditis elegans, Mus musculus, and Homo sapiens). In this paper we describe an EST project, which aims to investigate gene expression in Meloidogyne incognita at the onset of parasitism. Approximately 1000 5′-end sequence tags were produced from a cDNA library made of freshly hatched preparasitic second stage juveniles (J2). The EST were identified in the primary transformants of the cDNA library, and assigned to nine different functional groups, including (candidate) parasitism genes. A large fraction of the EST (45%) did not have a putative homologue in public databases. Sixty five percent of the EST that could be clustered into a functional group had putative homologues in other nematode species. EST were found for virtually all parasitism related genes that have been cloned from M. incognita to date. In addition, several novel genes were tagged, including a xylanase and a chitinase gene. The efficiency of EST projects, which produce sequence data for thousands of genes in months time without any difficult pre-selections of mRNA pools, makes random sequencing cDNA libraries a superior method to identify candidates for parasitism related genes in plant-parasitic nematodes. The sequences in this paper are retrievable from Genbank with the accession numbers BE191640 to BE191741, BE217592 to BE217720, BE225324 to BE225598, BE238852 to BE239221, and BE240829 to BE240865.

scholarly journals Chromatin landscape dynamics in development of the plant parasitic nematode Meloidogyne incognita

2021 ◽  
Author(s):  
Rahim Hassanaly-Goulamhoussen ◽  
Ronaldo De Carvalho Augusto ◽  
Nathalie Marteu-Garello ◽  
Arthur Péré ◽  
Bruno Favery ◽  
...  
Keyword(s):  
Gene Expression ◽  
Histone Modifications ◽  
Meloidogyne Incognita ◽  
Developmental Stages ◽  
Model Organisms ◽  
Parasitic Nematodes ◽  
Epigenetic Mechanisms ◽  
Root Knot Nematode ◽  
Dynamic Regulation ◽  
Plant Parasitic

In model organisms, epigenome dynamics underlies a plethora of biological processes. The role of epigenetic modifications in development and parasitism in nematode pests remains unknown. The root-knot nematode Meloidogyne incognita adapts rapidly to unfavorable conditions, despite its asexual reproduction. However, the mechanisms underlying this remarkable plasticity and their potential impact on gene expression remain unknown. This study provides the first insight into contribution of epigenetic mechanisms to this plasticity, by studying histone modifications in M. incognita. The distribution of five histone modifications revealed the existence of strong epigenetic signatures, similar to those found in the model nematode Caenorhabditis elegans. We investigated their impact on chromatin structure and their distribution relative to transposable elements (TE) loci. We assessed the influence of the chromatin landscape on gene expression at two developmental stages: eggs, and pre-parasitic juveniles. H3K4me3 histone modification was strongly correlated with high levels of expression for protein-coding genes implicated in stage-specific processes during M. incognita development. We provided new insights in the dynamic regulation of parasitism genes kept under histone modifications silencing. In this pioneering study, we establish a comprehensive framework for the importance of epigenetic mechanisms in the regulation of the genome expression and its stability in plant-parasitic nematodes.

scholarly journals Evaluation of the Effect of Oil Palm Bunch Ash on Meloidogyne incognita Infections on Soybean (Glycine max)

2016 ◽  
Vol 5 (2) ◽  
pp. 55-60
Author(s):  
Solomon Ogwulumba
Keyword(s):  
Glycine Max ◽  
Oil Palm ◽  
Meloidogyne Incognita ◽  
Block Design ◽  
Nematode Population ◽  
Growth And Yield ◽  
Root Knot Nematode ◽  
Gall Index ◽  
Cropping Seasons ◽  
Palm Bunch

An investigation to evaluate the effect of Oil Palm Bunch Ash on Meloidogyne incognita infections on soybean (Glycine max) was carried out at the Research and Teaching Farm of the Federal College of Agriculture Ishiagu, Ebonyi State, Nigeria, in 2012 and 2013 cropping seasons. The treatment was Oil Palm Bunch Ash at different levels, (control, 5tons/ha, 8tons/ha and 11tons/ha) and was fitted into a Randomized Complete Block Design (RCBD) with three replications. Data collected were averaged on plant height (cm), number of leaves, number of galled roots, and number of galls per root (gall index) and initial nematode population and final nematode population at harvest. The data were subjected to statistical analysis of variance. Results showed that Oil Palm Bunch as at 11tons/ha produced the greatest control for root-knot nematode by significantly (P0.05) reducing the number of galled root and galls per root at harvest (gall index). Results also revealed that nematode population was high in the control plots. The growth and yield parameters were significantly (P0.05) higher in the plots treated with 8tons/ha and 11tons/ha of Oil Palm Bunch Ash. It is therefore, recommended that oil palm bunch ash from 11tons/ha could be employed by farmers to suppress root-knot nematode in soybean field and increase soybean yield.

scholarly journals Chromatin Landscape Dynamics in the Early Development of the Plant Parasitic Nematode Meloidogyne incognita

2021 ◽  
Vol 9 ◽  
Author(s):  
Rahim Hassanaly-Goulamhoussen ◽  
Ronaldo De Carvalho Augusto ◽  
Nathalie Marteu-Garello ◽  
Arthur Péré ◽  
Bruno Favery ◽  
...  
Keyword(s):  
Gene Expression ◽  
Histone Modifications ◽  
Meloidogyne Incognita ◽  
Developmental Stages ◽  
Model Organisms ◽  
Parasitic Nematodes ◽  
Epigenetic Mechanisms ◽  
Root Knot Nematode ◽  
Dynamic Regulation ◽  
Plant Parasitic

In model organisms, epigenome dynamics underlies a plethora of biological processes. The role of epigenetic modifications in development and parasitism in nematode pests remains unknown. The root-knot nematode Meloidogyne incognita adapts rapidly to unfavorable conditions, despite its asexual reproduction. However, the mechanisms underlying this remarkable plasticity and their potential impact on gene expression remain unknown. This study provides the first insight into contribution of epigenetic mechanisms to this plasticity, by studying histone modifications in M. incognita. The distribution of five histone modifications revealed the existence of strong epigenetic signatures, similar to those found in the model nematode Caenorhabditis elegans. We investigated their impact on chromatin structure and their distribution relative to transposable elements (TE) loci. We assessed the influence of the chromatin landscape on gene expression at two developmental stages: eggs, and pre-parasitic juveniles. H3K4me3 histone modification was strongly correlated with high levels of expression for protein-coding genes implicated in stage-specific processes during M. incognita development. We provided new insights in the dynamic regulation of parasitism genes kept under histone modifications silencing. In this pioneering study, we establish a comprehensive framework for the importance of epigenetic mechanisms in the regulation of the genome expression and its stability in plant-parasitic nematodes.

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