Outcomes in culture positive and culture negative ascitic fluid infection in patients with viral cirrhosis: cohort study

Objectives: To determine the frequency of culture positive (SBP) and culture negative spontaneous bacterial peritonitis (neutrocytic ascites) in cirrhotic population. Period: The six months (November 08th 2012 to May 07th 2013). Study Design: Descriptive cross-sectional study. Setting: Department of Gastroenterology at Liaquat National Hospital. Total 107 patients of liver cirrhosis with ascites admitted in our hospital. All the specific patients had ascitic fluid DR and C/S were enrolled and evaluated. Patient’s information was recorded on proforma and analyzed by using SPSS-20.0. Results: SBP was detected in 10 7 patients aged between 18 and 67 years included in study. Out of 107 patients with SBP 23 (21.5%) were culture positive while 84 (78.5%) were culture negative. In the culture positive group,19 (82.6%) were male and 4 (17.4%) were female while in culture negative group 46 (54.76%) were male and 38 (45.24%) were female. The ascitic fluid mean total leukocyte count in patients with culture positive ascites was 5140.39 /mm3 and in culture negative ascites was 2654.26 / mm3. The ascitic fluid mean neutrophils count in subjects with culture positive ascites was 75.57% and in culture negative ascites was 76.02%. The ascitic fluid mean lymphocyte count in individuals had culture positive ascites was 26.09 % and in subjects had culture negative ascites was 23.97%. Conclusion: Frequency of culture negative ascites is greater than culture positive ascites in SBP. The ascitic fluid mean total leucocyte count for culture positive ascites is greater than culture negative ascites. Mortality is high in culture positive ascites.

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SPONTANEOUS BACTERIAL PERITONITIS

The Professional Medical Journal ◽

10.29309/tpmj/2006.13.02.5009 ◽

2006 ◽

Vol 13 (02) ◽

pp. 201-205

Author(s):

MUHAMMAD IMRAN ◽

Haroon-ur- Rashid ◽

SHOAIB NAIYAR HASHMI ◽

Assawar Hussain ◽

ASHFAQ ALTAF

Keyword(s):

Ascitic Fluid ◽

Spontaneous Bacterial Peritonitis ◽

Military Hospital ◽

Routine Examination ◽

E Coli ◽

Bacterial Peritonitis ◽

Cirrhotic Patients ◽

The Mean ◽

Culture Positive ◽

Culture Negative

Objective: To evaluate the prevalence, diagnostic criteria and microbialspectrum of spontaneous bacterial peritonitis (SBP) and its variants in cirrhotic patients with ascites. Design: A hospitalbased prospective study carried out in cirrhotic patients with ascites. Place and duration of Study: The study wasconducted in the Department of Medicine Military Hospital Rawalpindi during October 2000 to March 2002. Subjectand Methods: One hundred and eighty consecutive patients of cirrhotic liver between the ages of 20-65 yearspresenting with ascites reporting to outdoor patient department of Military Hospital Rawalpindi were included in thestudy. The diagnosis of cirrhosis was made on history, clinical examination and the ultrasonographic findings. Thesepatients were subjected to ascites fluid tap and the patients were divided into SBP and non-SBP groups in the light ofresults of ascitic fluid routine examination and culture. The SBP group was further categorized into culture positive SBP,culture negative neutrocytic ascites (CNNA) and bacterascites (BA) on the basis of culture results and total leukocytecount, absolute polymorphonuclear leukocyte count per cubic mm of ascitic fluid. Results: The study included 144males and 36 females having cirrhosis with ascites. The mean age of these patients was 52.32±7.87 years. On thebasis of routine examination and culture of tapped ascitic fluid from these cases 57 of 180(31.66%) patients werediagnosed to have SBP or its variants. In addition to classic SBP in 18(31.58%) patients, its variants namely culturenegative neutrocytic ascites and bacterascites were detected in 37(64.92%) and two (3.5%) cases respectively. E. coliwas the most frequently cultured organism. It was isolated in 12 cases of SBP (60%). In 4 cases (20%) Klebsiella wascultured whereas Proteus mirabilus and Streptococcus were reported in two cases (10%) each. Conclusion: This studyindicates that spontaneous bacterial peritonitis is common and potentially fatal complication in cirrhotic patients withascites. E. coli is the most frequent offending organism.

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Prosthetic joint infection in culture-negative and alpha-defensin-positive patients versus culture-positive and alpha-defensin-negative patients: a retrospective cohort study of the differences in clinical characteristics and outcomes

Current Orthopaedic Practice ◽

10.1097/bco.0000000000000942 ◽

2020 ◽

Vol 32 (1) ◽

pp. 58-63

Author(s):

Sravya P. Vajapey ◽

Daniel Li ◽

Daniel Lynch ◽

Mengnai Li

Keyword(s):

Cohort Study ◽

Prosthetic Joint Infection ◽

Retrospective Cohort Study ◽

Retrospective Cohort ◽

Clinical Characteristics ◽

Joint Infection ◽

Prosthetic Joint ◽

Culture Positive ◽

Culture Negative

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Prevalence of culture negative ascitic fluid infection among patients with chronic liver disease.

The Professional Medical Journal ◽

10.29309/tpmj/2020.27.05.4074 ◽

2020 ◽

Vol 27 (05) ◽

pp. 999-1003

Author(s):

Muhammad Ayub ◽

Sagheer Hussain ◽

Salman Ahmed ◽

Muhammad Adnan Iqbal

Keyword(s):

Liver Disease ◽

Chronic Liver Disease ◽

Ascitic Fluid ◽

Total Duration ◽

Positive Culture ◽

Chronic Liver ◽

Cross Sectional ◽

Male Patients ◽

Culture Positive ◽

Culture Negative

Objectives: To determine prevalence of patients with chronic liver disease having culture negative ascitic fluid infection. Study Design: Cross sectional study. Setting: Gastroenterology and medicine ward of DHQ Teaching Hospital Gujranwala. Period: January 2018 August 2018 having total duration of 8 months. Material & Methods: All patients irrespective of age and gender, having ascites due to chronic liver disease and showing signs and symptoms of ascitic fluid infection like fever, abdominal pain and tenderness, admitted in the medical unit of study hospital underwent ascitic tap and fluid was sent for culture examination to the hospital laboratory. Patients with chronic liver disease having no bacterial growth found on culture of ascitic fluid were placed in one group and who had culture positive ascites were placed in separate group. Patients who have taken any antibiotic in last one month or having any intra abdominal source of infection were excluded from the study. All cases in study group were already diagnosed with CLD. Results: There were 160 cases included in this study having chronic liver disease and ascitic fluid infection with 62.5% male and 37.5% female cases. Out of 160 cases, 22.5% were having culture positive ascites while 77.5% cases were having culture negative ascites among them 61% were male and 39% were female in positive group and 62.9% were male and 37.1% were female cases in negative group. Age range of patients was 25-75 years with mean age of 50±25 years. Mean duration of chronic liver disease was 9.5±2.4 months with minimum duration of 6 months and maximum duration of 15 months. There were 72.6% cases with culture negative ascites and 69.4% with positive culture were having age above 45 years. There was majority of male patients (62.9%) having culture negative ascites due to CLD. Conclusion: Ascitic fluid infection among patients with chronic liver disease is usually culture negative. In our study prevalence of culture negative ascitic flud infection was 77.5%, more common among male patients having age above 45 years.

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Bacteremic sepsis leads to higher mortality when adjusting for confounders with propensity score matching

Scientific Reports ◽

10.1038/s41598-021-86346-4 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Lisa Mellhammar ◽

Fredrik Kahn ◽

Caroline Whitlow ◽

Thomas Kander ◽

Bertil Christensson ◽

...

Keyword(s):

Intensive Care ◽

Clinical Review ◽

Pathogen Detection ◽

Mortality Rates ◽

Blood Cultures ◽

Population Heterogeneity ◽

Sepsis Diagnosis ◽

Culture Positive ◽

Culture Negative ◽

Microbial Samples

AbstractOne can falsely assume that it is well known that bacteremia is associated with higher mortality in sepsis. Only a handful of studies specifically focus on the comparison of culture-negative and culture-positive sepsis with different conclusions depending on study design. The aim of this study was to describe outcome for critically ill patients with either culture-positive or -negative sepsis in a clinical review. We also aimed to identify subphenotypes of sepsis with culture status included as candidate clinical variables. Out of 784 patients treated in intensive care with a sepsis diagnosis, blood cultures were missing in 140 excluded patients and 95 excluded patients did not fulfill a sepsis diagnosis. Of 549 included patients, 295 (54%) had bacteremia, 90 (16%) were non-bacteremic but with relevant pathogens detected and in 164 (30%) no relevant pathogen was detected. After adjusting for confounders, 90-day mortality was higher in bacteremic patients, 47%, than in non-bacteremic patients, 36%, p = 0.04. We identified 8 subphenotypes, with different mortality rates, where pathogen detection in microbial samples were important for subphenotype distinction and outcome. In conclusion, bacteremic patients had higher mortality than their non-bacteremic counter-parts and bacteremia is more common in sepsis when studied in a clinical review. For reducing population heterogeneity and improve the outcome of trials and treatment for sepsis, distinction of subphenotypes might be useful and pathogen detection an important factor.

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Culture-negative versus culture-positive in pyogenic spondylitis and analysis of risk factors for relapse

British Journal of Neurosurgery ◽

10.1080/02688697.2021.1896677 ◽

2021 ◽

pp. 1-5

Author(s):

Guohua Dai ◽

Shuzhong Li ◽

Chuqiang Yin ◽

Yuanliang Sun ◽

Jianwen Hou ◽

...

Keyword(s):

Risk Factors ◽

Pyogenic Spondylitis ◽

Culture Positive ◽

Culture Negative

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Blood culture-negative infective endocarditis: a worse outcome? Results from a large multicentre retrospective Spanish cohort study

Infectious Diseases ◽

10.1080/23744235.2021.1925342 ◽

2021 ◽

pp. 1-9

Author(s):

Lorenzo Roberto Suardi ◽

Arístides de Alarcón ◽

María Victoria García ◽

Antonio Plata Ciezar ◽

Carmen Hidalgo Tenorio ◽

...

Keyword(s):

Cohort Study ◽

Infective Endocarditis ◽

Blood Culture ◽

Culture Negative ◽

Spanish Cohort

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Association Between Diabetic Foot Infection Wound Culture Positivity and 1-Year Admission for Invasive Infection: A Multi-Center Cohort Study

Open Forum Infectious Diseases ◽

10.1093/ofid/ofab172 ◽

2021 ◽

Author(s):

Westyn Branch-Elliman ◽

Daniel Sturgeon ◽

Adolf W Karchmer ◽

Hillary J Mull

Keyword(s):

Diabetic Foot ◽

Systemic Infection ◽

Causative Organism ◽

Invasive Infection ◽

Diabetic Foot Ulcers ◽

Diabetic Foot Infections ◽

Culture Positivity ◽

Wound Culture ◽

Culture Positive ◽

Culture Negative

Abstract Inpatients with culture-positive diabetic foot infections are at elevated risk for subsequent invasive infection with the same causative organism. In outpatients with index diabetic foot ulcers, we found that wound culture positivity was independently associated with increased odds of 1-year admission for systemic infection when compared to culture-negative wounds.

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