scholarly journals Targeting surface nucleolin with multivalent HB-19 and related Nucant pseudopeptides results in distinct inhibitory mechanisms depending on the malignant tumor cell type

BMC Cancer ◽  
2011 ◽  
Vol 11 (1) ◽  
Author(s):  
Bernard Krust ◽  
Diala El Khoury ◽  
Isabelle Nondier ◽  
Calaiselvy Soundaramourty ◽  
Ara G Hovanessian
Keyword(s):  
Tumor Cell ◽  
Malignant Tumor ◽  
Cell Type ◽  
Inhibitory Mechanisms ◽  
Surface Nucleolin ◽  
Malignant Tumor Cell

Abstract 2238: Non-random genetic rearrangements driven highly malignant tumor-cell population dictates the development of high-risk neuroblastoma

2014 ◽  
Author(s):  
Faizan H. Khan ◽  
Satish K. Ramraj ◽  
Vijayabaskar Pandian ◽  
Sheeja Aravindan ◽  
Terence S. Herman ◽  
...  
Keyword(s):  
High Risk ◽  
Tumor Cell ◽  
Cell Population ◽  
Malignant Tumor ◽  
Tumor Cell Population ◽  
Malignant Tumor Cell ◽  
Genetic Rearrangements

Malignant tumor cell lines produce interleukin-1-like factor

1988 ◽  
Vol 24 (8) ◽  
pp. 753-758 ◽  
Author(s):  
Shinobu Miyauchi ◽  
Takamasa Moroyama ◽  
Seishi Kyoizumi ◽  
Jun-Ichi Asakawa ◽  
Tetsuji Okamoto ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Lines ◽  
Malignant Tumor ◽  
Interleukin 1 ◽  
Tumor Cell Lines ◽  
Malignant Tumor Cell

The Influence of Angiogeneic Gene Expression Following Management with Antineoplastic Agents to the Apoptosis of Malignant Tumor Cell

2004 ◽  
Vol 15 (3) ◽  
pp. 192
Author(s):  
Young Jae Kim ◽  
Yoon Young Lee ◽  
Young Me Lee ◽  
Sung Yeoul Chang ◽  
Seung Ryong Kim ◽  
...  
Keyword(s):  
Gene Expression ◽  
Tumor Cell ◽  
Malignant Tumor ◽  
Antineoplastic Agents ◽  
Malignant Tumor Cell

ChemInform Abstract: Synthesis of 7-Benzylamino-6-chloro-2-piperazino-4-pyrrolidinopteridine and Novel Derivatives Free of Positional Isomers. Potent Inhibitors of cAMP-Specific Phosphodiesterase and of Malignant Tumor Cell Growth.

ChemInform ◽  
2010 ◽  
Vol 30 (13) ◽  
pp. no-no
Author(s):  
Karl-Heinz Merz ◽  
Doris Marko ◽  
Thomas Regiert ◽  
Guido Reiss ◽  
Walter Frank ◽  
...  
Keyword(s):  
Cell Growth ◽  
Tumor Cell ◽  
Malignant Tumor ◽  
Positional Isomers ◽  
Tumor Cell Growth ◽  
Malignant Tumor Cell

scholarly journals Molecular Cloning, Expression and Purification of G-CSF Isoform D, an Alternative Splice Variant of Human G-CSF

2019 ◽  
Author(s):  
Fatemeh Sadat Sadat Toghraie ◽  
Mahsa Yazdanpanah-Samani ◽  
Elham Mahmoudi Maymand ◽  
Ahmad Hosseini ◽  
Amir Asgari ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Line ◽  
Malignant Tumor ◽  
Splice Variant ◽  
Fusion Proteins ◽  
Inclusion Bodies ◽  
Tumor Cell Line ◽  
Mrna Isoforms ◽  
Malignant Tumor Cell ◽  
First Time

Granulocyte colony-stimulating factor (G-CSF) is the major regulator of hemopoiesis and granulopoiesis. However, overexpression of G-CSF has been implicated in several important processes in tumor biology such as tumor growth, angiogenesis, and metastasis. Four different mRNA isoforms resulting from alternative splicing have been reported for G-CSF (transcript variants 1, 2, 3 and 4). The mRNAs and protein products of splice variants 1 and 2 have been isolated for the first time, from tumor cell lines. In the present study for the first time we isolated the G-CSF transcript variant 4 encoding G-CSF isoform D from a highly malignant tumor cell line (Mehr80) with overexpression of G-CSF. Both the full-length G-CSF isoform B and G-CSF isoform D were cloned from Mehr80 cell line, overexpressed in Escherichia coli as N-terminal glutathione-S-transferase fusion proteins in the form of inclusion bodies and affinity purified by the batch method using glutathione-Sepharose 4B resin. Both fusion proteins were successfully cloned and expressed. Folded recombinant proteins were solubilized from inclusion bodies using sarkosyl, Triton X-114 and CHAPS and purified. The purity of G-CSF isoforms was confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and they were clearly detected in western blot analysis using anti-G-CSF polyclonal antibody. The G-CSF plays various roles in physiological and pathological conditions, however to date, the differential function of G-CSF isoforms remains unknown. Considering the fact that G-CSF isoform D was isolated from a highly malignant tumor cell line with overexpression of G-CSF, the role of this splice variant in tumorigenesis requires further investigation.  

Detection of SNPs of Dck and Cda Genes in Five Hematological Malignant Tumor Cell Lines

Blood ◽  
2012 ◽  
Vol 120 (21) ◽  
pp. 5129-5129
Author(s):  
Zhang Xiaoping ◽  
Ziying Jian ◽  
Bao-An Chen ◽  
Peipei Xu ◽  
Miaoxin Peng ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Lines ◽  
Malignant Tumor ◽  
Conflicts Of Interest ◽  
Cytidine Deaminase ◽  
Raji Cell ◽  
Tumor Cell Lines ◽  
Nucleotide Polymorphisms ◽  
Malignant Tumor Cell ◽  
System Cell

Abstract Abstract 5129 Background & Aim: This study detected single nucleotide polymorphisms (SNPs) of deoxycytidine kinase(DCK) gene, cytidine deaminase(CDA) gene in five hematological malignant tumor cell lines, to find connections between sNPs and Cytarabine dose selection in the treatment of some hematopathy. Materials & Methods: Cell line K562, Ka, HL-60, U937, and Raji were cultured. The genomic DNA was isolated by QIAamp DNA Blood Mini kit. Designing primes were amplified by PCR to get the related DNA fragments. DCK gene A674G(rs111454937) C1644T(rs72552079), CDA gene A79C(rs2072671) G208A(rs60369023) was genotyped by means of matrix assisted laser desorption ionisation-time of flight mass spectrometry method (MALDI-TOF MS). Results: The genotype of locus A674G(rs111454937) on DCK gene in all five hematology system cell lines was AA; The genotype of locus C1644T(rs72552079) on DCK gene in all five hematology system cell lines was CC. The genotype of locus G208A(rs60369023) on CDA gene in all five hematology system cell lines was GG; For HL-60, U937, and Raji cell lines, the genotype of locus A79C(rs2072671) on CDA gene was AA, while for K562 and Ka cell lines, that was C/A. Conclusions: The genotype of locus A79C(rs2072671) on CDA gene was not all the same in the detected hematological malignant tumor cell lines, while the genotypes of the other 3 loci were same in the 5 cell lines. Disclosures: No relevant conflicts of interest to declare.

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