scholarly journals A novel blood collection device stabilizes cell-free RNA in blood during sample shipping and storage

2013 ◽  
Vol 6 (1) ◽  
Author(s):  
Jianbing Qin ◽  
Thomas L Williams ◽  
M Rohan Fernando
Keyword(s):  
Blood Collection ◽  
Collection Device ◽  
And Storage

scholarly journals Clinical Evaluation of the Torq Zero Delay Centrifuge System for Decentralized Blood Collection and Stabilization

Diagnostics ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1019
Author(s):  
Kyungjin Hong ◽  
Gabriella Iacovetti ◽  
Ali Rahimian ◽  
Sean Hong ◽  
Jon Epperson ◽  
...  
Keyword(s):  
Clinical Chemistry ◽  
Blood Collection ◽  
Test Results ◽  
Sample Collection ◽  
Rapid Separation ◽  
Cell Counts ◽  
Collection Device ◽  
Precision Study ◽  
Clinically Significant ◽  
Blood Specimens

Blood sample collection and rapid separation—critical preanalytical steps in clinical chemistry—can be challenging in decentralized collection settings. To address this gap, the Torq™ zero delay centrifuge system includes a lightweight, hand-portable centrifuge (ZDrive™) and a disc-shaped blood collection device (ZDisc™) enabling immediate sample centrifugation at the point of collection. Here, we report results from clinical validation studies comparing performance of the Torq System with a conventional plasma separation tube (PST). Blood specimens from 134 subjects were collected and processed across three independent sites to compare ZDisc and PST performance in the assessment of 14 analytes (K, Na, Cl, Ca, BUN, creatinine, AST, ALT, ALP, total bilirubin, albumin, total protein, cholesterol, and triglycerides). A 31-subject precision study was performed to evaluate reproducibility of plasma test results from ZDiscs, and plasma quality was assessed by measuring hemolysis and blood cells from 10 subject specimens. The ZDisc successfully collected and processed samples from 134 subjects. ZDisc results agreed with reference PSTs for all 14 analytes with mean % biases well below clinically significant levels. Results were reproducible across different operators and ZDisc production lots, and plasma blood cell counts and hemolysis levels fell well below clinical acceptance thresholds. ZDiscs produce plasma samples equivalent to reference PSTs. Results support the suitability of the Torq System for remotely collecting and processing blood samples in decentralized settings.

A Multicenter Evaluation of a Nongel Mechanical Separator Plasma Blood Collection Tube for Testing of Selected Therapeutic Drugs

2020 ◽  
Vol 5 (4) ◽  
pp. 671-685
Author(s):  
Svetlana Morosyuk ◽  
Julie Berube ◽  
Robert Christenson ◽  
Alan H B Wu ◽  
Denise Uettwiller-Geiger ◽  
...  
Keyword(s):  
The Other ◽  
Initial Time ◽  
Therapeutic Drug ◽  
Blood Collection ◽  
Test Results ◽  
Sample Storage ◽  
Therapeutic Drugs ◽  
Collection Tube ◽  
And Storage ◽  
Blood Collection Tube

Abstract Background Some therapeutic drugs are unstable during sample storage in gel tubes. BD Vacutainer® Barricor™ Plasma Blood Collection Tube with nongel separator was compared with plasma gel tubes, BD Vacutainer PST™, PST II, and BD Vacutainer Serum Tube for acetaminophen, salicylate, digoxin, carbamazepine, phenytoin, valproic acid, and vancomycin during sample storage for up to 7 days. Methods Seven hospital sites enrolled 705 participants who were taking at least one selected drug. The study tubes were collected and tested at initial time (0 h), after 48 h of storage at room temperature and on day 7 (after additional 5 days of refrigerated storage). The performance of BD Barricor tube was evaluated for each drug by comparing BD Barricor samples with samples from the other tubes at 0 h from the same participant; stability was evaluated by comparing test results from the same tube at 0 h, 48 h, and 7 days. Results At 0 h, BD Barricor showed clinically equivalent results for selected therapeutic drugs compared with the other tubes, except phenytoin in BD PST. Phenytoin samples ≥20 µg/mL in BD PST had 10–12% lower values than samples in BD Barricor. During sample storage, all selected drugs remained stable for 7 days in BD Barricor and in serum aliquots. In BD PST, all drugs remained stable except phenytoin and carbamazepine and in BD PST II except for phenytoin. Conclusion The BD Barricor Tube is effective for the collection and storage of plasma blood samples for therapeutic drug monitoring without sample aliquoting.

scholarly journals Preanalytical Determinants of Total and Free Prostate-Specific Antigen and Their Ratio: Blood Collection and Storage Conditions

1998 ◽  
Vol 44 (3) ◽  
pp. 685-688 ◽  
Author(s):  
Klaus Jung ◽  
Philipp von Klinggräff ◽  
Brigitte Brux ◽  
Pranav Sinha ◽  
Dietmar Schnorr ◽  
...  
Keyword(s):  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Storage Conditions ◽  
Blood Collection ◽  
And Storage

scholarly journals Blood Collection Tubes and Storage Temperature Should Be Evaluated when Using the Siemens ADVIA Centaur XP for Measuring 25-Hydroxyvitamin D

PLoS ONE ◽  
2016 ◽  
Vol 11 (11) ◽  
pp. e0166327
Author(s):  
Songlin Yu ◽  
Weiyan Zhou ◽  
Xinqi Cheng ◽  
Huiling Fang ◽  
Ruiping Zhang ◽  
...  
Keyword(s):  
Storage Temperature ◽  
Blood Collection ◽  
Hydroxyvitamin D ◽  
25 Hydroxyvitamin D ◽  
And Storage ◽  
Blood Collection Tubes

scholarly journals DNA Extract was from filter paper stored at room temperature v1

2021 ◽  
Author(s):  
Katie Izenour ◽  
Anwar Kalalah ◽  
Fayez Salib ◽  
Sarah not provided Zohdy
Keyword(s):  
Filter Paper ◽  
Whole Blood ◽  
Room Temperature ◽  
Cold Chain ◽  
Western World ◽  
Blood Collection ◽  
Conventional Pcr ◽  
Liquid Form ◽  
And Storage ◽  
Babesia Spp

Preservation of samples requiring cold-chain storage is an often unavoidable challenge especially when doing laboratory work outside the western world. Samples are a precious commodity and it is imperative to maintain their viability. One method for collection and storage of samples in a liquid form (blood, saliva, serum) at room temperature is on filter paper cards like the Cellabs Tropbio Filter Paper Blood Collection Disks™. Methods and protocols exist for the use and recovery of whole blood from filter paper discs. This protocol describes the wash and recovery of DNA extracted from whole blood using the Qiagen DNeasy Extraction kit and dried on filter paper, re-suspended after several months of room temperature storage and use in conventional PCR. This protocol was used to suspend DNA extracted from the wholeblood of Dogs in Cairo, Egypt. The DNA was extracted using the Qiagen DNEasy Extraction Kit and placed on Cellabs Tropbio Filter Paper Blood Collection Disks™ (filter paper). The DNA dried on the filter paper overnight and was stored in plastic self-closure baggies for several months before being washed off and used in Conventional PCR. The use case described here is of a dog who was PCR positive for Babesia spp. using a sample of whole blood washed from the filter paper as well as dried DNA from filter paper.

scholarly journals Rapid detection of SARS-CoV-2 antibodies in oral fluids

2020 ◽  
Author(s):  
Melanie A. MacMullan ◽  
Prithvi Chellamuthu ◽  
Aubree Mades ◽  
Sudipta Das ◽  
Fred Turner ◽  
...  
Keyword(s):  
Whole Blood ◽  
Rapid Detection ◽  
Oral Fluid ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Blood Collection ◽  
Protective Equipment ◽  
Oral Fluids ◽  
Collection Device ◽  
Detection Approach

AbstractCurrent commercially available methods for reliably detecting antibodies against SARS-CoV-2 remain expensive and inaccessible due to the need for whole blood collection by highly trained phlebotomists using personal protective equipment (PPE). We evaluated an antibody detection approach utilizing the OraSure® Technologies’ Oral Antibody Collection Device (OACD) and their proprietary SARS-CoV-2 total antibody detection enzyme-linked immunosorbent assay (ELISA). We found that the OraSure® test for total antibody detection in oral fluid had comparable sensitivity and specificity to serum-based ELISAs while presenting a more affordable and accessible system with the potential for self-collection.

scholarly journals A design of a multistage recycling device for Marine plastic waste

2020 ◽  
Vol 165 ◽  
pp. 05004
Author(s):  
Duokui Fang
Keyword(s):  
Plastic Waste ◽  
Plastic Pollution ◽  
Recovery Method ◽  
Effective Implementation ◽  
Multi Stage ◽  
Collection Device ◽  
Type Size ◽  
Size Grade ◽  
And Storage ◽  
Medium Diameter

Based on the research of Marine plastic garbage recovery method, the discussion of the recovery device and the understanding of the types of ships, this paper designs a multi-stage recovery device for Marine plastic garbage. This device according to the types of the existing Marine plastic garbage and recycling requirements, effective implementation for big (more than 50 cm diameter or width), medium (diameter and width are between 5 mm ~ 50 cm) and micro (diameter or width under 5 mm) type size grade plastic debris automatic classification recycling, determined the primary barb type crawler collection device, second level network intercept type garbage collection device and the third level based on the sponge and activated carbon micro plastic collection device. Recycling and storage of Marine plastic waste to effectively address Marine plastic pollution is consistent with the MARPOL convention on the protection of the Marine environment.

Generation of Kinins during Preparation and Storage of Leukoreduced Platelet Concentrates.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 947-947
Author(s):  
Marie Eve Moreau ◽  
Louis Thibault ◽  
Anik Désormeaux ◽  
François Marceau ◽  
Marie-Joëlle de Grandmont ◽  
...  
Keyword(s):  
Whole Blood ◽  
Platelet Rich Plasma ◽  
Storage System ◽  
Reference Population ◽  
Storage Conditions ◽  
Blood Collection ◽  
Converting Enzyme Inhibitors ◽  
Pharmacological Characterization ◽  
And Storage

Abstract BACKGROUND. Severe hypotensive reactions can occur following transfusion of blood components, particularly with platelets concentrates (PCs) leukoreduced with negatively- charged filters. Bradykinin (BK)-related peptides were proposed as a possible cause of this side-effect. This study evaluated the effects of leukoreduction and storage conditions on the levels of two kinins (BK and des-Arg9-BK) in PCs. METHODS. Whole blood donations (n=35) were processed using current PRP (platelet-rich plasma) procedure to prepare leukoreduced and unfiltered components by Leukotrap® RC-PL Whole Blood Collection, Filtration and Storage System. PCs and plasma were stored for 7 days at 20–24°C with agitation. Levels of BK and des-Arg9-BK were measured by specific enzyme immunoassays and HPLC at day 0, 2, 5 and 7 of storage. Mechanisms potentially responsible for accumulation of BK and des-Arg9-BK were studied. RESULTS. On day 0, kinins were measured in significantly higher concentrations in leukoreduced (BK: 101 ± 157 pg/mL; des-Arg9-BK: 194 ± 191 pg/mL) vs unfiltered PCs (BK: 71 ± 121 pg/mL; des-Arg9-BK: 98 ± 114 pg/mL). During storage, both kinins peaked on day 5, with concentrations higher than 1 ng/mL in 22% of leukoreduced as well as unfiltered PCs. Physicochemical and pharmacological characterization of immunoreactive kinins confirmed their nature. In vitro activation of the contact system of the corresponding platelet-poor plasma (PPP) showed that a high kinin concentration on day 5 of the storage corresponded to a low kinin-forming capacity of plasma. On day 7, BK was no longer elevated presumably due to its degradation and the depletion of kinin-forming capacity of the plasma in stored PCs. The activity of metallopeptidases that metabolize BK-related peptides in plasma from PCs were at levels similar to those recorded in the plasma of a normal reference population and were unaffected by storage. CONCLUSIONS. Our results indicate that filtration and storage conditions of PCs contribute to generation of pharmacologically relevant bradykinin levels that might pose a risk in susceptible patients. The clinical relevance of such high concentrations of kinins in PCs remains to be established but could potentially be significant especially in patients treated with angiotensin I-converting enzyme-inhibitors that affect the pathway of BK degradation.

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