Study of growth, metabolism, and morphology of Akkermansia muciniphila with an in vitro advanced bionic intestinal reactor

Abstract Background As a kind of potential probiotic, Akkermansia muciniphila abundance in human body is directly causally related to obesity, diabetes, inflammation and abnormal metabolism. In this study, A. muciniphila dynamic cultures using five different media were implemented in an in vitro bionic intestinal reactor for the first time instead of the traditional static culture using brain heart infusion broth (BHI) or BHI + porcine mucin (BPM). Results The biomass under dynamic culture using BPM reached 1.92 g/L, which improved 44.36% compared with the value under static culture using BPM. The biomass under dynamic culture using human mucin (HM) further increased to the highest level of 2.89 g/L. Under dynamic culture using porcine mucin (PM) and HM, the main metabolites were short-chain fatty acids (acetic acid and butyric acid), while using other media, a considerable amount of branched-chain fatty acids (isobutyric and isovaleric acids) were produced. Under dynamic culture Using HM, the cell diameters reached 999 nm, and the outer membrane protein concentration reached the highest level of 26.26 μg/mg. Conclusions This study provided a preliminary theoretical basis for the development of A. muciniphila as the next generation probiotic.

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Study of growth, metabolism, and morphology of Akkermansia muciniphila with an in vitro advanced bionic intestinal reactor

10.1101/2020.07.06.190843 ◽

2020 ◽

Author(s):

Zhi-tao Li ◽

Guo-ao Hu ◽

Li Zhu ◽

Zheng-long Sun ◽

Yun-Jiang ◽

...

Keyword(s):

Fatty Acids ◽

Brain Heart Infusion ◽

Short Chain Fatty Acids ◽

Static Culture ◽

Akkermansia Muciniphila ◽

Dynamic Culture ◽

Abnormal Metabolism ◽

Branched Chain ◽

Chain Fatty Acids

AbstractAs a kind of potential probiotic, Akkermansia muciniphila abundance in human body is directly causally related to obesity, diabetes, inflammation and abnormal metabolism. In this study, A. muciniphila dynamic cultures using five different media were implemented in an in vitro bionic intestinal reactor for the first time instead of the traditional static culture using brain heart infusion broth (BHI) or BHI + porcine mucin (BPM). The biomass under dynamic culture using BPM reached 1.92 g/L, which improved 44.36% compared with the value under static culture using BPM. The biomass under dynamic culture using human mucin (HM) further increased to the highest level of 2.89 g/L. Under dynamic culture using porcine mucin (PM) and HM, the main metabolites were short-chain fatty acids (acetic acid and butyric acid), while using other media, a considerable amount of branched-chain fatty acids (isobutyric and isovaleric acids) were produced. Under dynamic culture Using HM, the cell diameters reached 999 nm, and the outer membrane protein concentration reached the highest level of 26.26 μg/mg. This study provided a preliminary theoretical basis for the development of A. muciniphila as the next generation probiotic.

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The impact of long-term dietary pattern of fecal donor on in vitro fecal fermentation properties of inulin

Food & Function ◽

10.1039/c5fo00987a ◽

2016 ◽

Vol 7 (4) ◽

pp. 1805-1813 ◽

Cited By ~ 9

Author(s):

Junyi Yang ◽

Devin J. Rose

Keyword(s):

Fatty Acids ◽

Short Chain Fatty Acids ◽

Processed Meats ◽

Branched Chain Fatty Acids ◽

Fiber Plant ◽

Branched Chain ◽

The Impact ◽

Chain Fatty Acids

A diet high in whole grains, dry beans, and certain vegetables that contributed dietary fiber, plant protein, and B vitamins resulted in high short chain fatty acids, while a diet high in diary and processed meats that provided cholesterol and little fiber resulted in high branched chain fatty acids and ammonia during fecal fermentation of inulin.

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Short-Chain Fatty Acids Prevent Diabetic Nephropathy In Vivo and In Vitro

Diabetes ◽

10.2337/db18-92-or ◽

2018 ◽

Vol 67 (Supplement 1) ◽

pp. 92-OR ◽

Cited By ~ 1

Author(s):

WEI HUANG ◽

YONG XU ◽

YOUHUA XU ◽

LUPING ZHOU ◽

CHENLIN GAO

Keyword(s):

Fatty Acids ◽

Diabetic Nephropathy ◽

Short Chain Fatty Acids ◽

Short Chain ◽

Chain Fatty Acids

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Chicken-eaters and pork-eaters have different gut microbiota and tryptophan metabolites

Scientific Reports ◽

10.1038/s41598-021-91429-3 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Jie Shi ◽

Di Zhao ◽

Fan Zhao ◽

Chong Wang ◽

Galia Zamaratskaia ◽

...

Keyword(s):

Fatty Acids ◽

Gut Microbiota ◽

Short Chain Fatty Acids ◽

Short Chain ◽

Branched Chain Fatty Acids ◽

Tryptophan Metabolites ◽

Branched Chain ◽

Spearman’S Correlation ◽

Gut Microbiota Composition ◽

Chain Fatty Acids

AbstractThis study was aimed to evaluate the differences in the composition of gut microbiota, tryptophan metabolites and short-chain fatty acids in feces between volunteers who frequently ate chicken and who frequently ate pork. Twenty male chicken-eaters and 20 male pork-eaters of 18 and 30 years old were recruited to collect feces samples for analyses of gut microbiota composition, short-chain fatty acids and tryptophan metabolites. Chicken-eaters had more diverse gut microbiota and higher abundance of Prevotella 9, Dialister, Faecalibacterium, Megamonas, and Prevotella 2. However, pork-eaters had higher relative abundance of Bacteroides, Faecalibacterium, Roseburia, Dialister, and Ruminococcus 2. In addition, chicken-eaters had high contents of skatole and indole in feces than pork-eaters, as well as higher contents of total short chain fatty acids, in particular for acetic acid, propionic acid, and branched chain fatty acids. The Spearman’s correlation analysis revealed that the abundance of Prevotella 2 and Prevotella 9 was positively correlated with levels of fecal skatole, indole and short-chain fatty acids. Thus, intake of chicken diet may increase the risk of skatole- and indole-induced diseases by altering gut microbiota.

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Profiles of Odd- and Branched-Chain Fatty Acids and Their Correlations With Rumen Fermentation Parameters, Microbial Protein Synthesis and Bacterial Populations Based on Pure Carbohydrate Incubation in Vitro

10.21203/rs.3.rs-68439/v1 ◽

2020 ◽

Author(s):

Hangshu Xin ◽

Xin Liu ◽

Xin Jiang ◽

Chunlong Liu ◽

Shuzhi Zhang ◽

...

Keyword(s):

Fatty Acids ◽

Volatile Fatty Acids ◽

Cellulolytic Bacteria ◽

Bacterial Populations ◽

Branched Chain Fatty Acids ◽

Degrading Bacteria ◽

Branched Chain ◽

Fermentation Parameters ◽

Chain Fatty Acids

Abstract Background: The objectives of this study were to evaluate the profiles of odd- and branched-chain fatty acids (OBCFA; including C15:0, iso-C15:0, anteiso-C15:0, iso-C16:0, C17:0, iso-C17:0 and anteiso-C17:0) during pure carbohydrates incubation in vitro and whether they correlated with ruminal fermentation parameters, microbial crude protein (MCP) synthesis, and bacterial populations. The pure substrates containing five different ratios of fiber and starch (F:S; 0:100, 25:75, 50:50, 75:25 and 100:0) were incubated for 6 h, 12 h, 18 h and 24 h. Results: Except iso-C17:0, OBCFA concentrations were interacted by F:S and incubation time. The highest concentration of total OBCFA was found in the fermented mixture after 24 h of incubation when the F:S = 0:100; while the lowest level was 1.65 mg/g DM produced after 6 h of incubation with F:S = 50:50. The concentrations of total volatile fatty acids (TVFA) and MCP remarkably decreased linearly as the inclusion of fiber in the substrates increased, as expected. The proportions of investigated cellulolytic bacteria in our study were increased linearly (or linearly and quadratically) while those of R. amylophilus and S. bovis were decreased as fiber inclusion increased. The correlation analysis indicated that iso-C16:0 concentration might have potential as a marker of productions of TVFA and MCP with ρ being 0.78 and 0.82 respectively. Compared to starch degrading bacteria, cellulolytic bacteria had more correlations with OBCFA profiles, and the strongest association was found on the population of R. flavefaciens with C15:0 concentration (ρ = 0.70). Conclusions: Our study shows there might be scope for iso-C16:0 to predict rumen productions of VFA and MCP. Notedly, this is the first paper reporting linkage of OBCFA with rumen function based on pure carbohydrate in vitro incubation, which would avoid confounding interference from dietary protein and fat presence. However, more in-depth experiments are needed to substantiate the current findings.

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