scholarly journals The cold - resistance mechanism of a mutagenic Volvariella volvacea strain VH3 with outstanding traits revealed by transcriptome profiling

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Peng Li ◽  
Cong Hu ◽  
Yujie Li ◽  
Lei Ge ◽  
Guogan Wu ◽  
...  
Keyword(s):  
Transcriptome Sequencing ◽  
Cold Resistance ◽  
Cold Treatment ◽  
Resistance Mechanism ◽  
Transcriptome Profiling ◽  
Economic Loss ◽  
Volvariella Volvacea ◽  
Expression Change ◽  
Ultraviolet Mutagenesis ◽  
Straw Mushroom

Abstract Background The straw mushroom (Volvariella volvacea) is one of the important vegetables that is popular for its delicious taste. However, the straw mushroom is sensitive to low temperature, resulting in economic loss during transportation and storage. We obtained a novel straw mushroom strain, named VH3, via ultraviolet mutagenesis. Results Our study revealed that VH3 exhibited high cold resistance compared to an ordinary straw mushroom cultivar, V23. We found that the electrolyte leakages of VH3 were always significantly lower than that of V23 treated with 4 °C for 0 h, 2 h,4 h, 8 h, 16 h, and 24 h. Before cold treatment (0 h), there were no difference of MDA contents, SOD activities, and CAT activities between VH3 and V23. At the late stage (8 h, 26 h, and 24 h) of cold treatment, the MDA contents of VH3 were lower while both the SOD and CAT activities were higher than those of V23. To investigate the potential mechanisms of VH3 cold resistance, we performed transcriptome sequencing to detect the transcriptome profiling of VH3 and V23 after 0 h and 4 h cold treatment. Transcriptome sequencing revealed that 111 differentially expressed genes (DEG) between V23 (0 h) and VH3 (0 h) (V23–0_vs_VH3–0), consisting 50 up-regulated and 61 down-regulated DEGs. A total of 117 DEGs were obtained between V23 (4 h) and VH3(4 h) (V23–4_vs_VH3–4), containing 94 up-regulated and 23 down-regulated DEGs. Among these DEGs, VVO_00021 and VVO_00017 were up-regulated while VVO_00003, VVO_00004, VVO_00010, and VVO_00030 were down-regulated in V23–0_vs_VH3–0 and VH3–4_vs_V23–4. KEGG and GO analysis revealed that the 6 DEGs were annotated to pathways related to cold stress. Besides, the GA3 content was also decreased in VH3. Conclusions Collectively, our study first revealed that the increased cold resistance of VH3 might be caused by the expression change of VVO_00003, VVO_00004, VVO_00017, VVO_00021, and VVO_00030, and decreased GA3.

scholarly journals Comparative Transcriptome Analysis Reveals Potential Gene Modules Associated with Cold Tolerance in Sugarcane (Saccharum officinarum L.)

2021 ◽  
Author(s):  
Xing Huang ◽  
Yongsheng Liang ◽  
Baoqing Zhang ◽  
Xiupeng Song ◽  
Yangrui Li ◽  
...  
Keyword(s):  
Cold Stress ◽  
Cold Tolerance ◽  
Cold Resistance ◽  
Soluble Sugar ◽  
Resistance Mechanism ◽  
Saccharum Officinarum ◽  
Calcium Signal ◽  
Protein Kinase Activity ◽  
Comparative Transcriptome ◽  
Climate Conditions

AbstractSugarcane is an important crop worldwide, and most sugar is derived directly from sugarcane. Due to its thermophilic nature, the yield of sugarcane is largely influenced by extreme climate conditions, especially cold stress. Therefore, the development of sugarcane with improved cold tolerance is an important goal. However, little is known about the multiple mechanisms underlying cold acclimation at the bud stage in sugarcane. In this study, we emphasized that sensitivity to cold stress was higher for the sugarcane variety ROC22 than for GT42, as determined by physical signs, including bud growth capacity, relative conductivity, malonaldehyde contents, and soluble sugar contents. To understand the factors contributing to the difference in cold tolerance between ROC22 and GT42, comparative transcriptome analyses were performed. We found that genes involved in the regulation of the stability of the membrane system were the relative determinants of difference in cold tolerance. Additionally, genes related to protein kinase activity, starch metabolism, and calcium signal transduction were associated with cold tolerance. Finally, 25 candidate genes, including 23 variety-specific and 2 common genes, and 7 transcription factors were screened out for understanding the possible cold resistance mechanism. The findings of this study provide candidate gene resources for cold resistance and will improve our understanding of the regulation of cold tolerance at the bud stage in sugarcane.

scholarly journals The role of endogenous enzyme from straw mushroom (Volvariella volvacea) in improving taste and volatile flavor characteristics of Cantonese sausage

LWT ◽  
2021 ◽  
pp. 112627
Author(s):  
Xuping Wang ◽  
Pengfei Zhou ◽  
Jingrong Cheng ◽  
Huaigu Yang ◽  
Jinhao Zou ◽  
...  
Keyword(s):  
Volvariella Volvacea ◽  
Volatile Flavor ◽  
Straw Mushroom

scholarly journals Comparative Transcriptome Profiling Reveals Defense-Related Genes against Meloidogyne incognita Invasion in Tobacco

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 2081 ◽  
Author(s):  
Xiaohui Li ◽  
Xuexia Xing ◽  
Pei Tian ◽  
Mingzhen Zhang ◽  
Zhaoguang Huo ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Meloidogyne Incognita ◽  
Resistance Mechanism ◽  
Transcriptome Profiling ◽  
Ethylene Response Factor ◽  
Sequencing Analysis ◽  
Cell Wall Modification ◽  
Resistance Response ◽  
Helix Loop Helix ◽  
Genes Encoding

Root-knot nematodes Meloidogyne incognita are one of the most destructive pathogens, causing severe losses to tobacco productivity and quality. However, the underlying resistance mechanism of tobacco to M. incognita is not clear. In this study, two tobacco genotypes, K326 and Changbohuang, which are resistant and susceptible to M. incognita, respectively, were used for RNA-sequencing analysis. An average of 35 million clean reads were obtained. Compared with their expression levels in non-infected plants of the same genotype, 4354 and 545 differentially expressed genes (DEGs) were detected in the resistant and susceptible genotype, respectively, after M. incognita invasion. Overall, 291 DEGs, involved in diverse biological processes, were common between the two genotypes. Genes encoding toxic compound synthesis, cell wall modification, reactive oxygen species and the oxidative burst, salicylic acid signal transduction, and production of some other metabolites were putatively associated with tobacco resistance to M. incognita. In particular, the complex resistance response needed to overcome M. incognita invasion may be regulated by several transcription factors, such as the ethylene response factor, MYB, basic helix–loop–helix transcription factor, and indole acetic acid–leucine-resistant transcription factor. These results may aid in the identification of potential genes of resistance to M. incognita for tobacco cultivar improvement.

Cardiovascular active substances from the straw mushroom,Volvariella volvacea

1995 ◽  
Vol 9 (2) ◽  
pp. 93-99 ◽  
Author(s):  
K. W. Chiu ◽  
A. H. W. Lam ◽  
P. K. T. Pang
Keyword(s):  
Volvariella Volvacea ◽  
Straw Mushroom ◽  
Active Substances

scholarly journals Small GTPases and Stress Responses of vvran1 in the Straw Mushroom Volvariella volvacea

2016 ◽  
Vol 17 (9) ◽  
pp. 1527 ◽  
Author(s):  
Jun-Jie Yan ◽  
Bin Xie ◽  
Lei Zhang ◽  
Shao-Jie Li ◽  
Arend van Peer ◽  
...  
Keyword(s):  
Stress Responses ◽  
Small Gtpases ◽  
Volvariella Volvacea ◽  
Straw Mushroom

Nutritional and Medicinal Value of Specialty Mushrooms

1990 ◽  
Vol 53 (10) ◽  
pp. 883-894 ◽  
Author(s):  
WILLIAM M. BREENE
Keyword(s):  
Agaricus Bisporus ◽  
Lentinula Edodes ◽  
Blood Platelet ◽  
Toxic Substances ◽  
Volvariella Volvacea ◽  
Button Mushroom ◽  
Medicinal Value ◽  
Therapeutic Value ◽  
Straw Mushroom ◽  
Active Substances

Although the button mushroom (Agaricus bisporus) accounts for slightly over half of total world mushroom production, specialty mushrooms, e.g., shiitake (Lentinula edodes), straw (Volvariella volvacea), oyster (Pleurotus spp.), and enokitake (Flammulina velutipes), are increasing in popularity. These species contain moderate quantities of good quality protein and are good sources of dietary fiber, vitamin C, B vitamins, and minerals. Lipid levels are low, but unsaturated to saturated fatty acid ratios are high (about 2.0 – 4.5:1). Some species (e.g., shiitake) accumulate cadmium and selenium and other heavy metals, and some may contain toxic substances such as the heat labile cardiotoxic proteins volvatoxin in the straw mushroom and flammutoxin in enokitake. Extensive clinical studies, primarily in Japan, have clearly demonstrated that a number of species have medicinal and therapeutic value, by injection or oral administration, in the prevention/treatment of cancer, viral diseases (influenza, polio), hypercholesterolemia, blood platelet aggregation, and hypertension. Most of the studies have focused on shiitake, enokitake, Pleurotus spp., and on the generally nonculinary Ganoderma spp. Many of the active substances which include polysaccharides (e.g., β-glucans), nucleic acid derivatives (the hypocholesterolemic eritadenine), lipids, peptides, proteins, and glycoproteins, have been isolated and identified. Some of the mechanisms of activity have been elucidated, e.g., antiviral activity via stimulation of interferon production in the host. Additional medical claims less well documented may nonetheless have some validity and merit further study.

scholarly journals Sensitivity in the antioxidant system of discus fish (Symphysodon spp.) to cold temperature: evidence for species-specific cold resistance

2019 ◽  
Author(s):  
Shi-Rong Jin ◽  
Bin Wen ◽  
Zai-Zhong Chen ◽  
Jian-Zhong Gao ◽  
Lei Wang ◽  
...  
Keyword(s):  
Cold Hardiness ◽  
Cold Resistance ◽  
Cold Treatment ◽  
Principal Component ◽  
Interspecific Variation ◽  
Continuous Sequence ◽  
Model Animal ◽  
Set Up ◽  
Species Specific ◽  
Subordinate Function

ABSTRACTThe discus fish (Symphysodon spp.) is an endemic species of the Amazon that is among the most popular ornamental fish around the world, and is usually used as the model animal for studying the diversification of Amazon fish. Here, a comparative analysis of two species of discus fish, i.e., S. haraldi and S. aequifasciatus, based on several antioxidant indexes was conducted, to test the hypothesis that cold resistance might correlate with the diversification of discus fish. We set up a continuous sequence of three temperature programs, namely cooling (28 °C to 14 °C; -1 °C/h), cold maintenance (14 °C for 12 h) and recovery (14 °C to 28 °C; +1 °C/h). Subordinate function (SF) combined with principal component analysis (PCA) showed that the cold hardiness of S. haraldi during cold treatment was in the order of cooling > cold maintenance ≈ recovery, but the cold hardiness of S. aequifasciatus during cold treatment was in the order of cold maintenance > cooling > recovery. Specifically, the lowest cold hardiness was observed in S. aequifasciatus during recovery, indicating that cold stress resulted in more seriously oxidative stress in S. aequifasciatus than in S. haraldi. Overall, these results show a significant interspecific variation, indicating the correlation between environmental adaptation and the diversification of discus fish.

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