Genome-wide identification of the tobacco GDSL family and apical meristem-specific expression conferred by the GDSL promoter

Abstract Background GDSL esterases/lipases are a large protein subfamily defined by the distinct GDSL motif, and play important roles in plant development and stress responses. However, few studies have reported on the role of GDSLs in the growth and development of axillary buds. This work aims to identify the GDSL family members in tobacco and explore whether the NtGDSL gene contributes to development of the axillary bud in tobacco. Results One hundred fifty-nine GDSL esterase/lipase genes from cultivated tobacco (Nicotiana tabacum) were identified, and the dynamic changes in the expression levels of 93 of these genes in response to topping, as assessed using transcriptome data of topping-induced axillary shoots, were analysed. In total, 13 GDSL esterase/lipase genes responded with changes in expression level. To identify genes and promoters that drive the tissue-specific expression in tobacco apical and axillary buds, the expression patterns of these 13 genes were verified using qRT-PCR. GUS activity and a lethal gene expression pattern driven by the NtGDSL127 promoter in transgenic tobacco demonstrated that NtGDSL127 is specifically expressed in apical buds, axillary buds, and flowers. Three separate deletions in the NtGDSL127 promoter demonstrated that a minimum upstream segment of 235 bp from the translation start site can drive the tissue-specific expression in the apical meristem. Additionally, NtGDSL127 responded to phytohormones, providing strategies for improving tobacco breeding and growth. Conclusion We propose that in tobacco, the NtGDSL127 promoter directs expression specifically in the apical meristem and that expression is closely correlated with axillary bud development.

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Tissue-specific expression patterns of nuclear receptors

NURSA Datasets ◽

10.1621/datasets.02001 ◽

2013 ◽

Author(s):

AL Bookout ◽

Y Jeong ◽

M Downes ◽

RT Yu ◽

RM Evans ◽

...

Keyword(s):

Nuclear Receptors ◽

Expression Patterns ◽

Specific Expression ◽

Tissue Specific ◽

Tissue Specific Expression

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Tissue Distribution of ACE2 Protein in Syrian Golden Hamster (Mesocricetus auratus) and Its Possible Implications in SARS-CoV-2 Related Studies

Frontiers in Pharmacology ◽

10.3389/fphar.2020.579330 ◽

2021 ◽

Vol 11 ◽

Author(s):

Voddu Suresh ◽

Deepti Parida ◽

Aliva P. Minz ◽

Manisha Sethi ◽

Bhabani S. Sahoo ◽

...

Keyword(s):

Expression Pattern ◽

Golden Hamster ◽

Expression Patterns ◽

Mesocricetus Auratus ◽

Syrian Golden Hamster ◽

Surface Epithelium ◽

Specific Expression ◽

Tissue Specific ◽

Tissue Specific Expression ◽

Specific Expression Pattern

The Syrian golden hamster (Mesocricetus auratus) has recently been demonstrated as a clinically relevant animal model for SARS-CoV-2 infection. However, lack of knowledge about the tissue-specific expression pattern of various proteins in these animals and the unavailability of reagents like antibodies against this species hampers these models’ optimal use. The major objective of our current study was to analyze the tissue-specific expression pattern of angiotensin-converting enzyme 2, a proven functional receptor for SARS-CoV-2 in different organs of the hamster. Using two different antibodies (MA5-32307 and AF933), we have conducted immunoblotting, immunohistochemistry, and immunofluorescence analysis to evaluate the ACE2 expression in different tissues of the hamster. Further, at the mRNA level, the expression of Ace2 in tissues was evaluated through RT-qPCR analysis. Both the antibodies detected expression of ACE2 in kidney, small intestine, tongue, and liver. Epithelium of proximal tubules of kidney and surface epithelium of ileum expresses a very high amount of this protein. Surprisingly, analysis of stained tissue sections showed no detectable expression of ACE2 in the lung or tracheal epithelial cells. Similarly, all parts of the large intestine were negative for ACE2 expression. Analysis of tissues from different age groups and sex didn’t show any obvious difference in ACE2 expression pattern or level. Together, our findings corroborate some of the earlier reports related to ACE2 expression patterns in human tissues and contradict others. We believe that this study’s findings have provided evidence that demands further investigation to understand the predominant respiratory pathology of SARS-CoV-2 infection and disease.

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Structural analysis and tissue-specific expression patterns of a novel salt-inducible NAC transcription factor gene fromNicotiana tabacumcv. Xanthi

The Journal of Horticultural Science and Biotechnology ◽

10.1080/14620316.2014.11513140 ◽

2014 ◽

Vol 89 (6) ◽

pp. 700-706 ◽

Cited By ~ 6

Author(s):

Q. Q. Han ◽

P. Qiao ◽

Y. Z. Song ◽

J. Y. Zhang

Keyword(s):

Transcription Factor ◽

Structural Analysis ◽

Expression Patterns ◽

Nac Transcription Factor ◽

Transcription Factor Gene ◽

Specific Expression ◽

Tissue Specific ◽

Factor Gene ◽

Tissue Specific Expression

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The Murine Genome Contains Two Functional Kininogen Genes Leading to Tissue Specific Expression of High Molecular Weight Kininogen (HK) and Expression of a Novel HK Splice Variant.

Blood ◽

10.1182/blood.v104.11.3979.3979 ◽

2004 ◽

Vol 104 (11) ◽

pp. 3979-3979

Author(s):

Sergei Merkoulov ◽

Anton A. Komar ◽

Keith R. McCrae

Keyword(s):

Molecular Weight ◽

High Molecular Weight ◽

Expression Patterns ◽

Endothelial Cell Proliferation ◽

Head Orientation ◽

High Molecular Weight Kininogen ◽

Specific Expression ◽

Tissue Specific ◽

Tissue Specific Expression ◽

Exon 10

Abstract High molecular weight kininogen (HK) plays an important role in the assembly and activation of the kallikrein/kinin system. While the human genome contains only a single copy of the kininogen gene, three copies are present in the rat (one K-kininogen and two T-kininogen). Here, we report that the mouse genome contains two homologous kininogen genes (overall homology 91%), denoted mHK1 and mHK2. Both genes are located on chromosome 16 in a head-to-head orientation, and contain open reading frames. The size of intronic sequences between the 11 kininogen gene exons is similar (Figure). HK mRNA transcripts derived from the mHK1 and mHK2 genes differ slightly in size due to gaps of 33 and 18 nucleotides in exon 10 of mHK2. RT-PCR analysis of HK gene expression in adult and embryonic murine tissues revealed that HK mRNA was derived from mHK1 in liver, adrenal and embryo, but from mHK2 in kidney and lung. HK mRNA derived from both genes was present in testis, brain and muscle, though expression levels were low relative to those in other tissues. HK mRNA was not detected in ovary, bone marrow, heart or bladder. mHK1-derived HK mRNA was alternatively spliced, as demonstrated by the presence of an HK mRNA transcript encoding a novel HK1 isoform, ΔmD5, that lacked the portion of exon 10 encoding Thr400 - Asp582 of HK domains 5 and 6. Examination of the putative promoter regions of the two genes using the MatInspector Professional program (Genomatix) demonstrated distinct differences, perhaps explaining in part their tissue-specific expression patterns. Like domain 5 of human HK (hD5), domain 5 of murine HK (mD5), in which the histidine and lysine-rich C-terminal region of this domain previously shown to mediate the antiangiogenic activity of domain 5 is highly conserved, inhibited endothelial cell proliferation. While the function of each of the kininogen genes in the intact animal has yet to be defined, characterization of the two genes may provide new information concerning the role of high molecular weight kininogen in development, normal physiology, and pathological processes. Figure Figure

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Tissue-Specific Expression Patterns of MicroRNA during Acute Graft-versus-Host Disease in the Rat

Frontiers in Immunology ◽

10.3389/fimmu.2016.00361 ◽

2016 ◽

Vol 7 ◽

Cited By ~ 11

Author(s):

Dasaradha Jalapothu ◽

Margherita Boieri ◽

Rachel E. Crossland ◽

Pranali Shah ◽

Isha A. Butt ◽

...

Keyword(s):

Graft Versus Host Disease ◽

Expression Patterns ◽

Specific Expression ◽

Tissue Specific ◽

Host Disease ◽

Graft Versus Host ◽

Tissue Specific Expression ◽

Acute Graft

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Tissue-specific expression of PPAR mRNAs in diabetic rats and divergent effects of cilostazol

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y08-043 ◽

2008 ◽

Vol 86 (7) ◽

pp. 465-471 ◽

Cited By ~ 16

Author(s):

Furong Wang ◽

Ling Gao ◽

Bendi Gong ◽

Jianting Hu ◽

Mei Li ◽

...

Keyword(s):

Mrna Expression ◽

Renal Cortex ◽

Expression Patterns ◽

Diabetic Rats ◽

Diabetic Patients ◽

Peroxisome Proliferator ◽

Specific Expression ◽

Camp Content ◽

Tissue Specific ◽

Tissue Specific Expression

Cilostazol and ligands of peroxisome proliferator-activated receptors (PPARs) have been effectively used to alleviate diabetic complications, but the common and tissue-specific expression patterns of PPARs in different tissues in diabetic patients and those treated with cilostazol have not been reported. Here, we aimed to assess the effects of diabetes and cilostazol on mRNA expression of PPARα and PPARγ in the aorta, renal cortex, and retina of diabetic rats treated with cilostazol for 8 weeks. PPARα mRNA expression showed uniform downregulation in all these tissues in diabetic rats, and this effect was reversed by cilostazol treatment. Surprisingly, PPARγ mRNA expression was reduced in the renal cortex and retina, yet increased in the aorta of diabetic rats, although cilostazol still reversed these changes. Interestingly, cilostazol, a well-known phosphodiesterase 3 inhibitor and cAMP elevator, augmented cAMP content only in the aorta, but showed no significant effects in the renal cortex of diabetic rats. In conclusion, mRNA expression of PPARs is tissue-specific in diabetes and may be differently affected by cilostazol, possibly because of its tissue-specific effects on cAMP content.

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Differential expression of the closely linked KISS1, REN, and FLJ10761 genes in transgenic mice

Physiological Genomics ◽

10.1152/physiolgenomics.00205.2003 ◽

2004 ◽

Vol 17 (1) ◽

pp. 4-10 ◽

Cited By ~ 9

Author(s):

Ravi Nistala ◽

Xiaoji Zhang ◽

Curt D. Sigmund

Keyword(s):

Transgenic Mice ◽

Human Chromosome ◽

Expression Profiles ◽

Expression Patterns ◽

Artificial Chromosome ◽

Chromosome 1 ◽

Specific Expression ◽

Tissue Specific ◽

Tissue Specific Expression ◽

Human Chromosome 1

We previously reported the development and characterization of transgenic mice containing a large 160-kb P1 artificial chromosome (PAC) encompassing the renin (REN) locus from human chromosome 1. Here we demonstrate that PAC160 not only encodes REN, but also complete copies of the next upstream (KISS1) and downstream ( FLJ10761 ) gene along human chromosome 1. Incomplete copies of the second upstream (PEPP3) and downstream (SOX13) genes are also present. The gene order PEPP3-KISS1-REN-FLJ10761-SOX13 is conserved in mice containing either one or two copies of the REN locus. Despite the close localization of KISS1, REN, and FLJ10761 , they each exhibit distinct, yet overlapping tissue-specific expression profiles in humans. The tissue-specific expression patterns of REN and FLJ10761 were retained in transgenic mice containing PAC160. Expression of REN and FLJ10761 were also proportional to copy number. Expression of KISS1 in PAC160 mice showed both similarities and differences to humans. These data suggest that expression of gene blocks encoded on large genomic clones are retained when the clones are used to generate transgenic mice. Genomic elements which act to insulate genes from their neighbors are also apparently retained.

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