Establishing community-wide DNA barcode references for conserving mangrove forests in China

Abstract Background Mangrove ecosystems have been the focus of global attention for their crucial role in sheltering coastal communities and retarding global climate change by sequestering ‘blue carbon’. China is relatively rich in mangrove diversity, with one-third of the ca. 70 true mangrove species and a number of mangrove associate species occurring naturally along the country’s coasts. Mangrove ecosystems, however, are widely threatened by intensifying human disturbances and rising sea levels. DNA barcoding technology may help protect mangrove ecosystems by providing rapid species identification. Results To investigate this potential, 898 plant specimens were collected from 33 major mangrove sites in China. Based on the morphologic diagnosis, the specimens were assigned to 72 species, including all 28 true mangrove species and all 12 mangrove associate species recorded in China. Three chloroplast DNA markers rbcL, trnH-psbA, matK, and one nuclear marker ITS2 were chosen to investigate the utility of using barcoding to identify these species. According to the criteria of barcoding gaps in genetic distance, sequence similarity, and phylogenetic monophyly, we propose that a single marker, ITS2, is sufficient to barcode the species of mangroves and their associates in China. Furthermore, rbcL or trnH-psbA can also be used to gather supplement confirming data. In using these barcodes, we revealed a very low level of genetic variation among geographic locations in the mangrove species, which is an alert to their vulnerability to climate and anthropogenic disturbances. Conclusion We suggest using ITS2 to barcode mangrove species and terrestrial coastal plants in South China. The DNA barcode sequences we obtained would be valuable in monitoring biodiversity and the restoration of ecosystems, which are essential for mangrove conservation.

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Distribusi Scylla spp. di perairan estuari Sungai Donan Segara Anakan Bagian Timur, Cilacap

Habitus Aquatica ◽

10.29244/haj.2.1.1 ◽

2021 ◽

Vol 2 (1) ◽

Author(s):

Sulistiono Sulistiono ◽

Nurul Musyariafah Yahya ◽

Etty Riani

Keyword(s):

Economic Value ◽

Scylla Serrata ◽

Mangrove Forests ◽

Mud Crab ◽

Mangrove Species ◽

Central Java ◽

Rhizophora Apiculata ◽

Mangrove Ecosystems ◽

Avicennia Alba ◽

Mangrove Crabs

Mangrove forests are ecosystems that make up coastal areas and river estuaries. The examples of mangrove ecosystems found in Indonesia are in Segara Anakan, Central Java. One of the aquatic biota with the economic value found in the mangrove forest ecosystem is the mud crab (Scylla spp.). The purpose of this study was to assess the distribution of mangrove crabs (Scylla spp.) to the presence of mangrove species in the mouth of the Donan River, Segara Anakan. A sampling of crabs and observations of mangrove vegetation were carried out at five stations spread from the river to the sea. The results showed that there were three types of mangrove crabs caught in the vicinity of the study, namely Scylla tranquebarica, S. olivacea, and S. serrata. The types of mangroves found include Rhizophora apiculata, R. mucronata, Avicennia rumphiana, A. alba, A. officinalis, and Nypa. Regression analysis showed that mangrove density correlated with the abundance of Scylla tranquebarica and Scylla olivacea, but contradicts the abundance of Scylla serrate. PCA analysis showed that the Crab species Scylla tranquebarica and Scylla olivacea were associated with mangroves of Avicennia alba, Avicennia rumphiana, and Rhizophora apiculata. Meanwhile, Scylla serrata crabs are associated with Avicennia officinalis.

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Kondisi mangrove di pesisir timur pulau Jemaja Kepuluan Anambas

Dinamika Lingkungan Indonesia ◽

10.31258/dli.7.2.p.70-80 ◽

2020 ◽

Vol 7 (2) ◽

pp. 70

Author(s):

Wahyudi Andrito ◽

Syafruddin Nasution ◽

Efriyeldi Efriyeldi

Keyword(s):

East Coast ◽

Good Condition ◽

Canopy Cover ◽

Coastal Areas ◽

Average Density ◽

Survey Method ◽

Mangrove Forests ◽

Bruguiera Gymnorrhiza ◽

Mangrove Species ◽

Sea Levels

Mangrove ecosystems are one of three important ecosystems in the coastal areas that supports global warming mitigation efforts. The existence of mangrove forests is needed to reduce the increased CO2 concentrations in the atmosphere. However, the rise of the water temperatures and sea levels indirectly affects the presence and condition of mangrove forests in coastal areas. The purpose of this study is to determine the condition of the mangrove community on the East Coast of Jemaja, Anambas Archipelagoes Regency. The survey method was used in this study and a series survey activities were conducted for a quadrant transect with a size of 10m by 10m in December 2019 - March 2020.The result shown that the five mangrove species were identified, such as Rhizophora apiculata, R. mucronata, Bruguiera gymnorrhiza, Ceriops tagal and Xylocarpus granatum. R. apiculata dominates with an important value index of 123.12%. While, the average density of mangroves and saplings in the study area was 3155 ± 785 individuals/ha and the average canopy cover was 84.03 ± 3.11%. With this finding, it can be concluded that the condition of mangroves in the East Coast of Jemaja is very dense and still in a good condition.

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Potential of Mangrove Ecosystem as a Tourism Object Development in Kaledupa Island

Edutourism Journal Of Tourism Research ◽

10.53050/ejtr.v2i01.124 ◽

1970 ◽

Vol 2 (01) ◽

pp. 11-17

Author(s):

Amiruddin Akbar Fisu ◽

Afandi Ahmad ◽

Adrianto Hidayat ◽

Liza Utami Marzaman

Keyword(s):

Mangrove Ecosystem ◽

Marine Resources ◽

Mangrove Forests ◽

Fish Ponds ◽

Mangrove Species ◽

Human Needs ◽

Mangrove Ecosystems ◽

Coral Reef Ecosystems ◽

Seagrass Ecosystems ◽

Evaluative Research

Mangrove forests that live in coastal areas, besides being beneficial for human needs, also often clash with development interests such as ports, fish ponds, and tourist areas that threaten their sustainability. Wakatobi Regency has the potential of living natural resources in the form of coastal and marine resources which are quite large, including mangrove ecosystems, seagrass ecosystems and coral reef ecosystems. The purpose of this study is to analyze the potential of mangrove ecosystems and to design the development of mangrove forests as an ecotourism potential. This research is a descriptive-evaluative research, with a qualitative approach. This research is important as a consideration in the development of attractions, which are the main sector in Wakatobi Regency. Mangrove species that dominate throughout the Wakatobi region are Rhizophora spp, Avicennia spp. and Sonneratia spp. Based on the potential of each island in Wakatobi Regency, Kaledupa Island is a priority area to be developed into ecotourism. This study recommends the construction of several supporting facilities for ecotourism on Kaledupa Island. Suggestions and infrastructure include service facilities, trade facilities and accommodation facilities

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Typhoons exert significant but differential impact on net carbon ecosystem exchange of subtropical mangrove ecosystems in China

Biogeosciences Discussions ◽

10.5194/bgd-11-9421-2014 ◽

2014 ◽

Vol 11 (6) ◽

pp. 9421-9449

Author(s):

H. Chen ◽

W. Lu ◽

G. Yan ◽

S. Yang ◽

G. Lin

Keyword(s):

Ecosystem Respiration ◽

Global Climate ◽

Co2 Exchange ◽

Climate Change Scenarios ◽

Strong Wind ◽

Mangrove Forests ◽

Local Cooling ◽

Mangrove Ecosystems ◽

Typhoon Season ◽

Carbon Dioxide Co2

Abstract. Typhoons are very unpredictable natural disturbances to subtropical mangrove forests in Asian countries, but litter information is available on how these disturbances affect ecosystem level carbon dioxide (CO2) exchange of mangrove wetlands. In this study, we examined short-term effect of frequent strong typhoons on defoliation and net ecosystem CO2 exchange (NEE) of subtropical mangroves, and also synthesized 19 typhoons during a 4-year period between 2009 and 2012 to further investigate the regulation mechanisms of typhoons on ecosystem carbon and water fluxes following typhoon disturbances. Strong wind and intensive rainfall caused defoliation and local cooling effect during typhoon season. Daily total NEE values were decreased by 26–50% following some typhoons (e.g. W28-Nockten, W35-Molave and W35-Lio-Fan), but were significantly increased (43–131%) following typhoon W23-Babj and W38-Megi. The magnitudes and trends of daily NEE responses were highly variable following different typhoons, which were determined by the balance between the variances of gross ecosystem production (GEP) and ecosystem respiration (RE). Furthermore, results from our synthesis indicated that the landfall time of typhoon, wind speed and rainfall were the most important factors controlling the CO2 fluxes following typhoon events. These findings not only indicate that mangrove ecosystems have strong resilience to the frequent typhoon disturbances, but also demonstrate the damage of increasing typhoon intensity and frequency on subtropical mangrove ecosystems under future global climate change scenarios.

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Reproductive Biology of Mangrove Plants Clerodendrum inerme, Derris trifoliata, Suaeda maritima, Suaeda monoica, Suaeda nudiflora

Transylvanian Review of Systematical and Ecological Research ◽

10.1515/trser-2015-0092 ◽

2016 ◽

Vol 18 (3) ◽

pp. 31-68 ◽

Cited By ~ 1

Author(s):

Jacob Solomon Raju Aluri ◽

Rajendra Kumar ◽

Prasada Rao Chappidi

Keyword(s):

Breeding System ◽

Floral Biology ◽

Breeding Systems ◽

Mangrove Forests ◽

Agricultural Activities ◽

Sexual System ◽

Suaeda Maritima ◽

External Agents ◽

Associate Species ◽

Mangrove Associate

Abstract Floral biology, sexual system, breeding system, pollinators, fruiting and seed dispersal aspects of five mangrove associate species, namely, Clerodendrum inerme, Derris trifoliata, Suaeda maritima, S. monoica, and S. nudiflora were studied. All these species are hermaphroditic, self-compatible, and exhibit mixed breeding systems adapted for pollination by external agents. C. inerme and D. trifoliata are principally entomophilous, while Suaeda species are ambophilous. The study suggests that these plant species are important constituents of mangrove forests. C. inerme and D. trifoliata are useful in stabilizing the banks of back water creeks, while Suaeda species are useful to carpet the saline areas and desalinate the soils which are subsequently useful for agricultural activities.

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Mangrove response to sea level rise: palaeoecological insights from macrotidal systems in northern Australia

Marine and Freshwater Research ◽

10.1071/mf17252 ◽

2018 ◽

Vol 69 (6) ◽

pp. 917 ◽

Cited By ~ 10

Author(s):

C. D. Woodroffe

Keyword(s):

Sea Level Rise ◽

Sea Level ◽

Mangrove Forests ◽

Northern Australia ◽

Mangrove Species ◽

Tidal Elevation ◽

Sea Levels ◽

Local Topography ◽

Accretion Rates ◽

Catch Up

Accelerated sea-level rise threatens coastal wetlands; it is unclear whether sediment accretion beneath mangroves will be sufficient to keep pace. A conceptual framework, used to describe the response of reefs, can also be applied to mangroves, discriminating drowning or back-stepping with rapid rise from keep-up or catch-up under moderate rates. In macrotidal estuaries of northern Australia, different mangrove species grow across particular elevation ranges and accretion rates decrease with tidal elevation. Palaeoecological reconstructions, from drilling, dating and pollen analysis, record mangrove distribution over past millennia. Estuarine plains are underlain by a vertically continuous stratigraphy of muds, implying continuity of widespread ‘big swamp’ mangrove forests during decelerating stages of post-glacial sea-level rise c. 7000 years ago. In contrast, on higher-energy open coasts, mangroves back-stepped, but re-established as the shoreline prograded when the nearshore built to suitable elevation: a catch-up mode. These results demonstrate that mangrove response to sea-level rise has varied, determined by the availability of sediment and the oceanographic processes by which it is redistributed. How mangrove forests adjust in future will also vary as a function of local topography and sediment availability. Extensive plains flanking estuarine systems are particularly vulnerable to tidal creek extension and saline incursion under future higher sea levels.

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Potential of Mangrove Ecosystem as a Tourism Object Development in Kaledupa Island

Edutourism Journal Of Tourism Research ◽

10.46964/jtr..v2i1.323 ◽

2020 ◽

Vol 2 (1) ◽

pp. 11

Author(s):

Amiruddin Akbar Fisu ◽

Afandi Ahmad ◽

Adrianto Hidayat ◽

Liza Utami Marzaman

Keyword(s):

Mangrove Ecosystem ◽

Marine Resources ◽

Mangrove Forests ◽

Fish Ponds ◽

Mangrove Species ◽

Human Needs ◽

Mangrove Ecosystems ◽

Coral Reef Ecosystems ◽

Seagrass Ecosystems ◽

Evaluative Research

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Keanekaragaman dan Pola Sebaran Moluska (Gastropoda dan Bivalvia) yang Berasosiasi Pada Ekosistem Mangrove di Pesisir Selatan Lombok Timur

Jurnal Sains Teknologi & Lingkungan ◽

10.29303/jstl.v0i0.274 ◽

2021 ◽

pp. 223-242

Author(s):

Wayan Pasek Eka Sedana Putra ◽

Abdul Syukur ◽

Didik Santoso

Keyword(s):

Diversity Index ◽

Pearson Correlation ◽

Mangrove Ecosystem ◽

Pearson Correlation Coefficient ◽

Mangrove Forests ◽

Southern Coast ◽

Mangrove Species ◽

Mangrove Ecosystems ◽

Independent Variable ◽

Mollusk Species

Mangrove ecosystems have an important role in coastal areas. Mangroves have a function as a shelter, foraging for food, and breeding various aquatic biota. Damage to mangrove forests can cause the service of mangrove ecosystems to decrease, especially for mollusk life. Mollusks are one of the associated biotas of mangroves whose life is influenced by mangrove forests. Therefore, the purpose of this study was to determine the diversity of mollusks and the relationship between mollusk density and mangrove density in the mangrove ecosystem of the southern coast of East Lombok. A sampling of mollusks used the quadrate sampling method by purposive sampling by taking into account the mangrove species contained in the observation plot. The collected data were analyzed by diversity index, distribution pattern, association, and Pearson correlation coefficient. Then a simple linear regression analysis was performed with the independent variable (x) mangrove density and the dependent variable (y) mollusk density. The results of this study were the discovery of 22 species of mollusks consisting of 17 species of gastropods and five species of bivalves. The density of mollusks in the mangrove ecosystem of the southern coast of East Lombok is 44.50±2.63 ind./m2. The diversity index at the study site was classified as moderate, with a value ranging from 1.99 to 2.46. The distribution pattern of mollusks at the research site is clustered with an Id value > 1. There are eight mollusk species associated with three mangrove species from 22 mollusk species found. The linear regression equation y = 0.084x – 0.2963 with a Pearson correlation coefficient (r) 0.95 describes a strong relationship between the independent variable (x) mangrove density and the dependent variable (y) mollusk density. This study concludes that there is a significant relationship between mangrove density and mollusk density as indicated by F-hit(41.01) > F-table (7.70) and P-value (0.003) < (0.05).

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Leaf Enzyme and Plant Productivity Responses to Environmental Stress Associated with Sea Level Rise in Two Asian Mangrove Species

Forests ◽

10.3390/f10030250 ◽

2019 ◽

Vol 10 (3) ◽

pp. 250 ◽

Cited By ~ 1

Author(s):

Xiaobo Lv ◽

Donghai Li ◽

Xiaobo Yang ◽

Mengwen Zhang ◽

Qin Deng

Keyword(s):

Sea Level Rise ◽

Sea Level ◽

Synergistic Effects ◽

High Tide ◽

Mangrove Forests ◽

Mangrove Species ◽

Sea Levels ◽

Tidal Flooding ◽

Seawater Salinity ◽

Flooding Time

As the only forests situated at the transition between land and sea, mangrove forests are one of the first ecosystems vulnerable to rising sea levels. When the sea level rises, plants are exposed to increased salinity, as well as tidal flooding. The responses of mangrove forests to changing sea levels depend on the synergistic effects of tidal flooding and salinity on plants, especially seedlings. The focus of this paper is to assess the ability of different tide position on mangrove Aegiceras corniculatum (A. corniculatum) and Bruguiera sexangula (B. sexangula) seedlings to withstand tidal flooding and seawater salinity, and to investigate the effects of tidal flooding and salinity on plant growth. To accomplish this, a controlled experiment was initiated to examine the synergistic effects of tidal flooding and salinity on the growth and physiology of A. corniculatum and B. sexangula seedlings subjected to four tidal flooding times and four levels of salinity over a course of six months. The results showed that the biomass and antioxidant enzymes of A. corniculatum and B. sexangula seedlings were significantly affected by the increase in salinity and flooding time. Changes in biomass, SOD, and CAT activity of A. corniculatum seedlings show that they are more adapted to grow in an environment with high salinity and long flooding time than B. sexangula. Our results show that species growing in middle- to low-tide levels were better adapted to sea level rise than those growing at high-tide levels.

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Isolation and amplification of mangrove plants using DNA barcode in Percut Sei Tuan, North Sumatra, Indonesia

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/912/1/012028 ◽

2021 ◽

Vol 912 (1) ◽

pp. 012028

Author(s):

R Syahbana ◽

M Basyuni ◽

L A M Siregar

Keyword(s):

Dna Isolation ◽

Dna Barcode ◽

Mangrove Forests ◽

Mangrove Species ◽

Nypa Fruticans ◽

Rhizophora Apiculata ◽

Living Things ◽

Acanthus Ilicifolius ◽

Avicennia Alba ◽

North Sumatra

Abstract Mangroves are a collection of several species of trees or shrubs that distribute around the coastline and can survive in high salinity environments. Around 60% of mangrove forests in North Sumatra are reported to have been damaged, the main factors of this damage being the mangrove forests conversion into ponds and the expansion of oil palm plantations. Identification of mangrove species is very important in protecting and applying the biodiversity of mangrove forests. Identification of living things has evolved from morphological charcetrization to molecular identification. This study aims to explain the DNA isolation and PCR methods to identify mangrove species in North Sumatra. The results suggested that the rbcL primer used can detect mangrove species that were visualized in the form of DNA bands. The length of DNA fragments of mangrove species Acrosticum aureum ranged 632.0-619.6 bp, species Rhizophora apiculata 619.6-585.8 bp, species Nypa fruticans 600- 592.9 bp, species Avicennia alba 549.1-533.5 bp, species Hibiscus tiliaceus was not detected, and mangrove species Acanthus ilicifolius 480.3 bp.

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