scholarly journals Effects of different vasopressors on the contraction of the superior mesenteric artery and uterine artery in rats during late pregnancy

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Tingting Wang ◽  
Limei Liao ◽  
Xiaohui Tang ◽  
Bin Li ◽  
Shaoqiang Huang
Keyword(s):  
Caesarean Section ◽  
Blood Vessels ◽  
Mesenteric Artery ◽  
Uterine Artery ◽  
Contractile Response ◽  
Mesenteric Arteries ◽  
Response Curves ◽  
Pregnant Rats ◽  
Neuraxial Anaesthesia ◽  
Significant Difference

Abstract Background Hypotension after neuraxial anaesthesia is one of the most common complications during caesarean section. Vasopressors are the most effective method to improve hypotension, but which of these drugs is best for caesarean section is not clear. We assessed the effects of vasopressors on the contractile response of uterine arteries and superior mesenteric arteries in pregnant rats to identify a drug that increases the blood pressure of the systemic circulation while minimally affecting the uterine and placental circulation. Methods Isolated ring segments from the uterine and superior mesenteric arteries of pregnant rats were mounted in organ baths, and the contractile responses to several vasopressor agents were studied. Concentration-response curves for norepinephrine, phenylephrine, metaraminol and vasopressin were constructed. Results The contractile response of the mesenteric artery to norepinephrine, as measured by the pEC50 of the drug, was stronger than the uterine artery (5.617 ± 0.11 vs. 4.493 ± 1.35, p = 0.009), and the contractile response of the uterine artery to metaraminol was stronger than the mesenteric artery (pEC50: 5.084 ± 0.17 vs. 4.92 ± 0.10, p = 0.007). There was no statistically significant difference in the pEC50 of phenylephrine or vasopressin between the two blood vessels. Conclusions In vitro experiments showed that norepinephrine contracts peripheral blood vessels more strongly and had the least effect on uterine artery contraction. These findings support the use of norepinephrine in mothers between the time of neuraxial anaesthesia and the delivery of the foetus.

scholarly journals Effects of different vasopressors on the contraction of the superior mesenteric artery and uterine artery in rats during late pregnancy

2021 ◽  
Author(s):  
Tingting Wang ◽  
Limei Liao ◽  
Xiaohui Tang ◽  
Bin Li ◽  
Shaoqiang Huang
Keyword(s):  
Caesarean Section ◽  
Mesenteric Artery ◽  
Uterine Artery ◽  
Contractile Response ◽  
Mesenteric Arteries ◽  
Response Curves ◽  
Pregnant Rats ◽  
Neuraxial Anaesthesia ◽  
Significant Difference

Abstract Background：Hypotension after neuraxial anaesthesia is one of the most common complications during caesarean section. Vasopressors are generally agreed to be the most effective way to improve hypotension, but it is unclear which of these drugs is best for caesarean section. We assessed the effects of vasopressors on the contractile response of uterine arteries and superior mesenteric arteries in pregnant rats, with the goal of identifying a drug that raises the blood pressure of the systemic circulation while minimally affecting the uterine and placental circulation. Methods： Isolated ring segments from the uterine and superior mesenteric arteries of pregnant rats were mounted in organ baths, and their contractile responses to several vasopressor agents were studied. Concentration-response curves for norepinephrine, phenylephrine, metaraminol and vasopressin were constructed. Results：The experimental results showed that the contractile response of the mesenteric artery to norepinephrine, as measured by the pEC50 of the drug, was stronger than that of the uterine artery (5.617 ± 0.11 vs. 4.493 ± 1.35, p=0.009), and the contractile response of the uterine artery to metaraminol was stronger than that of the mesenteric artery (pEC50: 5.084±0.17 vs 4.92±0.10, p=0.007). There was no statistically significant difference in the pEC50 of phenylephrine or vasopressin between the two blood vessels. Conclusion： In vitro experiments show that compared with phenylephrine, metaraminol , vasopressin, norepinephrine can contract peripheral blood vessel more strongly, while having the least effect on the contraction of uterine artery. These findings provide some support for the use of norepinephrine in mothers between the time of neuraxial anaesthesia and the delivery of the foetus.

Correlation between mRNA levels and functional role of α1-adrenoceptor subtypes in arteries: evidence of α1L as a functional isoform of the α1A-adrenoceptor

2005 ◽  
Vol 289 (5) ◽  
pp. H1923-H1932 ◽  
Author(s):  
Daniel Martí ◽  
Raquel Miquel ◽  
Khalid Ziani ◽  
Regina Gisbert ◽  
M. Dolores Ivorra ◽  
...  
Keyword(s):  
Mesenteric Artery ◽  
Mesenteric Arteries ◽  
Main Role ◽  
Mrna Levels ◽  
Rt Pcr ◽  
Response Curves ◽  
Inhibitory Potency ◽  
Adrenoceptor Antagonist ◽  
Relevant Role

The mRNA levels for the three α1-adrenoceptor subtypes, α1A, α1B, and α1D, were quantified by real-time RT-PCR in arteries from Wistar rats. The α1D-adrenoceptor was prominent in both aorta (79.0%) and mesenteric artery (68.7%), α1A predominated in tail (61.7%) and small mesenteric artery (73.3%), and both α1A- and α1D-subtypes were expressed at similar levels in iliac artery. The mRNA levels of the α1B-subtype were a minority in all vessels (1.7–11.1%). Concentration-response curves of contraction in response to phenylephrine or relaxation in response to α1-adrenoceptor antagonists on maximal sustained contraction induced by phenylephrine were constructed from control vessels and vessels pretreated with 100 μmol/l chloroethylclonidine (CEC) for 30 min. The significant decrease in the phenylephrine potency observed after CEC treatment together with the inhibitory potency displayed by 8-{2-[4-(2-methoxyphenyl)-1-piperazinyl]-8-azaspiro ( 4 , 5 ) decane-7-dionedihydrochloride} (BMY-7378, an α1D-adrenoceptor antagonist) confirm the relevant role of α1D-adrenoceptors in aorta and iliac and proximal mesenteric arteries. The potency of 5-methylurapidil (an α1A-adrenoceptor antagonist) and the changes in the potency of both BMY-7378 and 5-methylurapidil after CEC treatment provided evidence of a mixed population of α1A- and α1D-adrenoceptors in iliac and distal mesenteric arteries. The low potency of prazosin (pIC50 < 9) as well as the high 5-methylurapidil potency in tail and small mesenteric arteries suggest the main role of α1A/α1L-adrenoceptors with minor participation of the α1D-subtype. The mRNA levels and CEC treatment corroborated this pattern and confirmed that the α1L-adrenoceptor could be a functional isoform of the α1A-subtype.

Endothelin-1 and -3 cause endothelium-dependent hyperpolarization in the rat mesenteric artery

1993 ◽  
Vol 265 (6) ◽  
pp. H2137-H2141 ◽  
Author(s):  
M. Nakashima ◽  
P. M. Vanhoutte
Keyword(s):  
Mesenteric Artery ◽  
Mesenteric Arteries ◽  
Receptor Subtype ◽  
Spontaneously Hypertensive ◽  
Rat Mesenteric Artery ◽  
Significant Difference ◽  
Eta Receptor ◽  
Etb Receptor ◽  
Wistar Kyoto ◽  
Eta Receptor Antagonist

The present study was designed to determine whether endothelin (ET) induces endothelium-dependent hyperpolarization in the isolated rat mesenteric artery and, if so, to identify the receptor subtype involved. Main superior mesenteric arteries of Wistar-Kyoto and spontaneously hypertensive rats were used for the measurement of electrical responses of smooth muscle cells, using glass microelectrode. In tissues with endothelium of both strains, ET-1 (10(-8) M) caused an initial transient hyperpolarization followed by a sustained depolarization. In tissues without endothelium, only depolarization was observed. ET-3 (10(-8) M) produced transient hyperpolarizations only in preparations with endothelium. There was no significant difference in maximal amplitude of hyperpolarization between the two strains. BQ-123 (selective ETA-receptor antagonist) blocked the depolarization to ET-1 but did not inhibit hyperpolarizing responses to either isopeptide. IRL-1620 (specific ETB-receptor agonist) produced transient membrane hyperpolarizations in tissues with endothelium. The hyperpolarizations induced by ET were not affected by NG-nitro-L-arginine. These data suggest that both ET-1 and ET-3 can cause endothelium-dependent hyperpolarization in the rat mesenteric artery and that the endothelial receptor involved may belong to ETB subtype.

scholarly journals Vasorelaxant Effect Induced by the Essential Oil of Ocotea duckei Vattimo Leaves and Its Main Constituent, Trans-caryophyllene, in Rat Mesenteric Artery

2020 ◽  
pp. 31-39
Author(s):  
Tays Amanda Felisberto Gonçalves ◽  
Renildo Moura da Cunha ◽  
Dionatas Ulises de Oliveira Meneguetti ◽  
Marcio Roberto Viana Santos ◽  
José Maria Barbosa- Filho ◽  
...  
Keyword(s):  
Essential Oil ◽  
Mesenteric Artery ◽  
Calcium Release ◽  
Mesenteric Arteries ◽  
Dependent Manner ◽  
Main Constituent ◽  
Response Curves ◽  
Vasorelaxant Effect ◽  
Rat Mesenteric Artery ◽  
Isolated Rat

Aims: To evaluate the vasorelaxant effect induced by the essential oil of the leaves of O. duckei Vattimo (ODEO) and its main constituent, trans-caryophyllene, in rat superior mesenteric arteries. Methodology: Isolated rat superior mesenteric rings were suspended by cotton threads for isometric tension recordings in Tyrode’s solution at 37ºC, gassed with 95% O2 and 5% CO2 and different ODEO concentrations (0.1-300 μg/mL) or trans-caryophyllene (1-1000 μg/mL) were added cumulatively to the organ baths. Results: Vasorelaxant effect induced by the essential oil of Ocotea duckei leaves (ODEO) and its main constituent, trans-caryophyllene (60.54 %), was evaluated in this work. In intact isolated rat superior mesenteric rings ODEO (0.1-300 μg/mL, n=6) induced concentration-dependent relaxation of tonus induced by phenylephrine (10 µM) or K+-depolarizing solution (KCl 80 mM) (IC50=31±5, 5±0.4 µg/mL, respectively, n=6). The relaxations of phenylephrine-induced contractions were not significantly attenuated after removal of the vascular endothelium (IC50=25±5 µg/mL). ODEO antagonized the concentration-response curves to CaCl2 (10-6-3x10-2 M) and Bay K 8644 (10-10-3x10-6 M). Furthermore, in nominally without calcium solution, ODEO significantly inhibited, in a concentration-dependent manner, transient contractions induced by 10 µM phenylephrine or 20 µM caffeine. Trans-caryophyllene induced vasorelaxations, however, this effect was 18.6 times less potent when compared to ODEO-induced vasorelaxations. Conclusion: The relaxant effect induced by ODEO in rat superior mesenteric artery rings is endothelium-independent and seems to be related to both, inhibition of Ca2+ influx through L-type voltage-gated Ca2+-channels sensitive to dihydropyridines and inhibition of the calcium release from intracellular IP3-and caffeine-sensitive stores.

scholarly journals Interaction of Perivascular Adipose Tissue and Sympathetic Nerves in Arteries From Normotensive and Hypertensive Rats

2016 ◽  
pp. S391-S399 ◽  
Author(s):  
J. TÖRÖK ◽  
A. ZEMANČÍKOVÁ ◽  
Z. KOCIANOVÁ
Keyword(s):  
Adipose Tissue ◽  
Electrical Stimulation ◽  
Mesenteric Artery ◽  
Sympathetic Nerves ◽  
Mesenteric Arteries ◽  
Inhibitory Influence ◽  
Hypertensive Rats ◽  
Response Curves ◽  
Perivascular Adipose Tissue ◽  
Endogenous Noradrenaline

The inhibitory action of perivascular adipose tissue (PVAT) in modulation of arterial contraction has been recently recognized and contrasted with the prohypertensive effect of obesity in humans. In this study we demonstrated that PVAT might have opposing effect on sympatho-adrenergic contractions in different rat conduit arteries. In superior mesenteric artery isolated from normotensive Wistar-Kyoto rats (WKY), PVAT exhibited inhibitory influence on the contractions to exogenous noradrenaline as well as to endogenous noradrenaline released from arterial sympathetic nerves during transmural electrical stimulation or after application of tyramine. In contrast, the abdominal aorta with intact PVAT responded with larger contractions to transmural electrical stimulation and tyramine when compared to the aorta after removing PVAT; the responses to noradrenaline were similar in both. This indicates that PVAT may contain additional sources of endogenous noradrenaline which could be responsible for the main difference in the modulatory effect of PVAT on adrenergic contractions between abdominal aortas and superior mesenteric arteries. In spontaneously hypertensive rats (SHR), the anticontractile effect of PVAT in mesenteric arteries was reduced, and the removal of PVAT completely eliminated the difference in the dose-response curves to exogenous noradrenaline between SHR and WKY. These results suggest that in mesenteric artery isolated from SHR, the impaired anticontractile influence of PVAT might significantly contribute to its increased sensitivity to adrenergic stimuli.

Norepinephrine and renin content in arterial tissue from different vascular beds

1969 ◽  
Vol 47 (1) ◽  
pp. 87-91 ◽  
Author(s):  
J. Genest ◽  
S. Simard ◽  
J. Rosenthal ◽  
R. Boucher
Keyword(s):  
Mesenteric Artery ◽  
Hind Limb ◽  
Rectus Abdominis ◽  
Mesenteric Arteries ◽  
Mesenteric Vein ◽  
Significant Difference ◽  
Arterial Tissue ◽  
Norepinephrine Content ◽  
Vascular Beds ◽  
Renin Content

Norepinephrine and renin contents in arterial tissues from different vascular territories in mongrel dogs were measured simultaneously. A significantly greater concentration of these two substances was found in the branches of the superior and inferior mesenteric arteries in contrast to arterial tissue taken from the aorta and from carotid, renal, femoral, and hind limb arteries of similar diameter. Norepinephrine and renin were undetectable in the rectus abdominis artery. There was no significant difference in the norepinephrine content of branches of mesenteric vein when compared with similar branches of mesenteric artery.

scholarly journals PO-139 Effects of Exercise During Pregnancy on CaV1.2 Channel in Mesenteric Artery from SHR Offspring

2018 ◽  
Vol 1 (4) ◽  
Author(s):  
Shan Shan Li ◽  
Fan Xing Zeng ◽  
Li Jun Shi
Keyword(s):  
Blood Pressure ◽  
Protein Expression ◽  
Isometric Contraction ◽  
Mesenteric Artery ◽  
Exercise Group ◽  
Mesenteric Arteries ◽  
Sedentary Group ◽  
Pressor Responses ◽  
Significant Difference ◽  
Exercise During Pregnancy

Objective To investigate the effect of exercise during pregnancy on L-type Ca2+ (CaV1.2) channel in mesenteric artery from spontaneously hypertensive rats (SHR) offspring. Methods Female (11 weeks old) and male (12 weeks old) SHR, female (11 weeks old) and male (12 weeks old) WKY rats were selected to use for breeding. The day when the vaginal bolt was found was considered day 1 of gestation. The pregnant rats were randomly divided into four groups: WKY sedentary group (WKY-SED), WKY exercise group (WKY-EX), SHR sedentary group (SHR-SED) and SHR exercise group (SHR-EX). The exercise groups were subjected to swimming at the first day of pregnancy, 1h/d，6 days/week for 3 weeks. The 6-month-old male offspring were operated with femoral arterial and venous cannulation, and the blood pressure after intravenous (i.v.) injection of CaV1.2 channel opener BayK8644 and blocker nifedipine were monitored in vivo. In vitro study, the mesenteric arteries were removed and used for isometric contraction studies. The non-selective NOS inhibitor Nω-nitro-L-arginine methyl ester (L-NAME, 100 μM) was added after 60mM KCl measurement. To investigate the contribution of CaV1.2 channels in vascular tone regulation, the vascular responses to nifedipine (10−9–10−5M) were examined. Western blot was applied to examine the protein expression levels of CaV1.2 channel. Results (1) The mean arterial pressure(MAP) were higher in the 6M offspring of SHR-SED group than that of WKY-SED group (P＜0.01), but there was no significant difference between the 6M offspring of SED and Ex groups. (2) The pressor responses induced by i.v. injection of BayK8644（0.1mg/Kg）were increased in the 6M offspring of SHR-SED group (P＜0.05) compared with the WKY-SED group. Exercise during pregnancy markedly decreased the pressor responses in 6M offspring of SHR-EX group (P＜0.05). (3) Compared with the 6M offspring of WKY-SED group, the depressor responses induced by i.v. injection of nifedipine（1mg/Kg）were increased in the 6M offspring of SHR-SED group (P＜0.01). Exercise during pregnancy markedly attenuated the depressor responses in 6M offspring of SHR-EX group (P＜0.05). (4) The isometric contraction study revealed that nifedipine induced concentration-dependent vasorelaxation in mesenteric artery precontracted with noradrenaline. The sensitivity of tissues to nifedipine in 6M offspring of SHR-SED group was significantly higher than that of WKY-SED group (P＜0.01). Exercise during pregnancy normalized the increased sensitivity of tissues to nifedipine in 6M offspring of SHR (P＜0.05). (5) Compared with the 6M offspring of WKY-SED group, the protein expression of CaV1.2α1C was significantly increased in SHR-SED group(P＜0.01). Exercise during pregnancy markedly inhibited the expression of CaV1.2α1C in 6M offspring of SHR-EX group (P＜0.05). Conclusions Pregnancy exercise has no significant effect on basic blood pressure in 6M offspring of SHR; but the increased function and protein expression of CaV1.2 channel in 6M offspring of SHR may be normalized by exercise during pregnancy.

Contraction and endothelium-dependent relaxation in mesenteric microvessels from pregnant rats

1995 ◽  
Vol 269 (6) ◽  
pp. H1899-H1904 ◽  
Author(s):  
I. F. Pascoal ◽  
M. D. Lindheimer ◽  
C. Nalbantian-Brandt ◽  
J. G. Umans
Keyword(s):  
Dose Response ◽  
Effective Concentration ◽  
Mesenteric Arteries ◽  
Half Maximum ◽  
Response Curves ◽  
Pregnant Rats ◽  
No Release ◽  
Dose Response Curves ◽  
Mesenteric Microvessels ◽  
Endothelium Dependent Relaxation

We assessed KCl- and phenylephrine (PE)-induced vasoconstriction as well as acetylcholine (ACh)-induced endothelium-dependent vasodilation in small, isometrically mounted mesenteric arteries from virgin and gravid rats, studied in the absence and presence of NG-nitro-L-arginine (L-NNA). Neither maximal vasoconstriction nor PE potency differed significantly between vessels from virgin and pregnant rats, either in the absence or presence of L-NNA. L-NNA resulted in similar twofold leftward shifts in the PE dose-response curves for both groups. ACh-induced relaxation was potentiated in vessels from gravid rats (half-maximum effective concentration = 0.25 vs. 0.04 microM, virgin and gravid rats, respectively). After L-NNA, maximal relaxation was inhibited significantly more in vessels from gravid rats (62 vs. 31%). Likewise, maximal slope of ACh dose-response curves and ACh potency were decreased in this group so that values no longer differed from those in virgins. We conclude that pregnancy does not alter basal nitric oxide (NO) synthesis in these isolated microvessels, but it does enhance ACh-induced NO release, while apparently inhibiting the action of a NO-independent, endothelium-derived vasodilator.

scholarly journals Comparative study to identify the safety, effectiveness and ease of obstetric forceps for delivery of floating head in cesarean section

2018 ◽  
Vol 7 (5) ◽  
pp. 1760 ◽  
Author(s):  
Sonali Jitendra Ingole ◽  
Saloni Manwani
Keyword(s):  
Caesarean Section ◽  
Blood Loss ◽  
Apgar Score ◽  
Uterine Artery ◽  
Fetal Head ◽  
Uterine Incision ◽  
Assisted Delivery ◽  
Significant Difference ◽  
Group 2 ◽  
Group 1

Background: Difficulty is frequently encountered in extraction of floating fetal head. This study will focus on comparison of Forceps assisted fetal head extraction during Lower segment caesarean section (LSCS) with manual method of extraction in LSCS.Methods: The ANC patients attending antenatal OPD and admitted for elective caesarean section fulfilling the inclusion criteria were randomly divided into two groups each of 400 patients: Group 1 of patients undergoing manual extraction of fetal head during LSCS; and Group 2 consisting of patients with forceps assisted delivery of fetal head in LSCS. Following factors will be evaluated in patients: maternal blood loss, any extension of uterine incision, difference in pre and post op hemoglobin levels of the patient and Apgar score of baby at 1 and 5 minutes.Results: Patients in both the groups were matched demographically. The demographic variables such as maternal age, weight, parity and MGA (Mean Gestational age) were comparable in both the groups. Blood loss was significant in Group 1 (manual delivery) as compared with Forceps assisted delivery. This is also reflected in difference in pre and post op Hemoglobin levels. Although baby outcome in terms of Apgar score was similar in both groups, however morbidity in terms of uterine artery trauma, extension of uterine incision was much less in group 2 (Forceps assisted LSCS delivery)Conclusions: Although there was no statistically significant difference in outcome of babies (APGAR score), complication(s) were less (blood loss, uterine artery trauma) in Forceps assisted LSCS delivery group. Proper selection of patient(s), early anticipation for application for Forceps can help for better outcome of caesarean delivery.

Pharmacological properties of canine intrapulmonary blood vessels

1984 ◽  
Vol 62 (9) ◽  
pp. 1198-1202 ◽  
Author(s):  
Siow-Kee Kong ◽  
Newman L. Stephens
Keyword(s):  
Pulmonary Artery ◽  
Pulmonary Vein ◽  
Contractile Response ◽  
Pulmonary Veins ◽  
Pulmonary Arteries ◽  
Pulmonary Vasculature ◽  
Maximal Response ◽  
Response Curves ◽  
Significant Difference ◽  
Differential Responsiveness

The contractile response of ring segments of large, medium, and small pulmonary arteries and veins of the dog to histamine, norepinephrine, and serotonin have been studied. The maximum contractile response to these drugs was normalized with respect to the maximal response obtained in stimulation with 127 mM K+. The small pulmonary artery was more reactive to histamine, norepinephrine, and serotonin when compared with large and medium pulmonary arteries. The medium and large pulmonary artery showed no difference in reactivity to histamine. However, the mean effective dose (ED50) values for these agonists among the different segments of pulmonary arteries showed no significant difference. The small and medium pulmonary veins demonstrated increased reactivity to histamine, but not to norepinephrine and serotonin. The ED50 values also indicated that both small and medium veins were more sensitive to histamine when compared with the large pulmonary vein. The log concentration percent response curves for both small and medium pulmonary veins were displaced leftward (increased sensitivity) with respect to that for the large pulmonary vein. However, the reactivity and sensitivity to histamine between medium and small pulmonary veins were no different. The reactivity and sensitivity of different segments of pulmonary veins to norepinehrine and serotonin showed no significant differences among them. We conclude that histamine and other vasoactive substances, which are directly or indirectly related to mast cell degranulation, exert pharmacological effects on the pulmonary vasculature which possesses differential responsiveness at various levels of the vascular tree.

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