scholarly journals Over-expression of a NAC 67 transcription factor from finger millet (Eleusine coracana L.) confers tolerance against salinity and drought stress in rice

2016 ◽  
Vol 16 (S1) ◽  
Author(s):  
Hifzur Rahman ◽  
Valarmathi Ramanathan ◽  
Jagedeeshselvam Nallathambi ◽  
Sudhakar Duraialagaraja ◽  
Raveendran Muthurajan
Keyword(s):  
Transcription Factor ◽  
Drought Stress ◽  
Finger Millet ◽  
Eleusine Coracana ◽  
Over Expression

scholarly journals Cloning and Expression Analysis of bZIP Transcription Factor from Finger Millet (Eleusine coracana L.) Under Abiotic Stress Conditions

2022 ◽  
Vol 9 (1) ◽  
pp. 1-13
Author(s):  
Jenifer Lolita C
Keyword(s):  
Transcription Factor ◽  
Salt Stress ◽  
Abiotic Stress ◽  
Drought Stress ◽  
Expression Analysis ◽  
Finger Millet ◽  
Abiotic Stress Tolerance ◽  
Eleusine Coracana ◽  
Stress Conditions ◽  
Cloning And Expression

Basic leucine zipper (bZIP) transcription factors comprise one of the largest gene families in plants. They play a key role in almost every aspect of plant growth and development and also in biotic and abiotic stress tolerance. In this study, we were attempted to study characterization of bZIP, a transcription factor from a climate smart cereal finger millet (Eleusine coracana L.). Seeds of Eleusine coracana (finger millet) was purchase from local market and were grown under field conditions drought and salt stress conditions. In this study, EcbZIP gene was isolated from finger millet, cloned into DH5α cells, screened by using colony PCR and expression analysis in response to two abiotic stresses was carried out by using qRT PCR. EcbZIP coding DNA sequence and protein sequence were retrieved from NCBI Nucleotide Database and Genpept of Accession number KP033192.1 and AJP67539.1 and validated by using SMART (simple modular architecture tool) Domain Tool. Cloning and expression studies were carried out using standardized molecular biology protocol. Results depicted that EcbZIP transcription factor showed significant upregulation under both salt and drought stress conditions, indicating that it plays an important role in tolerance towards these stresses. In conclusion, expression analysis of bZIP gene from finger millet seed cultivar ML-365 showed 5-fold upregulation to salt stress to drought stress and 8-fold upregulation to salt stress. Hence, it can serve as a candidate gene for improving abiotic stress tolerance and can be helpful in enhancing the crop productivity under stress conditions.

scholarly journals Integration of transcriptomic and proteomic analyses for finger millet [Eleusine coracana (L.) Gaertn.] in response to drought stress

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0247181
Author(s):  
Jiguang Li ◽  
Yanlan Wang ◽  
Liqun Wang ◽  
Jianyu Zhu ◽  
Jing Deng ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Finger Millet ◽  
Unsaturated Fatty Acids ◽  
Comparison Group ◽  
Eleusine Coracana ◽  
Differentially Expressed ◽  
Integrated Analysis ◽  
Carbohydrate Binding ◽  
Oxidoreductase Activity

Drought is one of the most significant abiotic stresses that affects the growth and productivity of crops worldwide. Finger millet [Eleusine coracana (L.) Gaertn.] is a C4 crop with high nutritional value and drought tolerance. However, the drought stress tolerance genetic mechanism of finger millet is largely unknown. In this study, transcriptomic (RNA-seq) and proteomic (iTRAQ) technologies were combined to investigate the finger millet samples treated with drought at different stages to determine drought response mechanism. A total of 80,602 differentially expressed genes (DEGs) and 3,009 differentially expressed proteins (DEPs) were identified in the transcriptomic and proteomic levels, respectively. An integrated analysis, which combined transcriptome and proteome data, revealed the presence of 1,305 DEPs were matched with the corresponding DEGs (named associated DEGs-DEPs) when comparing the control to samples which were treated with 19 days of drought (N1-N2 comparison group), 1,093 DEGs-DEPs between control and samples which underwent rehydration treatment for 36 hours (N1-N3 comparison group) and 607 DEGs-DEPs between samples which were treated with drought for 19 days and samples which underwent rehydration treatment for 36 hours (N2-N3 comparison group). Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis identified 80 DEGs-DEPs in the N1-N2 comparison group, 49 DEGs-DEPs in the N1-N3 comparison group, and 59 DEGs-DEPs in the N2-N3 comparison group, which were associated with drought stress. The DEGs-DEPs which were drought tolerance-related were enriched in hydrolase activity, glycosyl bond formation, oxidoreductase activity, carbohydrate binding and biosynthesis of unsaturated fatty acids. Co-expression network analysis revealed two candidate DEGs-DEPs which were found to be centrally involved in drought stress response. These results suggested that the coordination of the DEGs-DEPs was essential to the enhanced drought tolerance response in the finger millet.

scholarly journals Morpho-physiological and biochemical changes in finger millet [Eleusine coracana (L.) Gaertn.] under drought stress

2020 ◽  
Vol 26 (11) ◽  
pp. 2151-2171
Author(s):  
Lakshmipathi Naik Mude ◽  
Muniraja Mondam ◽  
Vijayalakshmi Gujjula ◽  
Sivakumar Jinka ◽  
Osman Basha Pinjari ◽  
...  
Keyword(s):  
Drought Stress ◽  
Finger Millet ◽  
Eleusine Coracana ◽  
Biochemical Changes ◽  
Physiological And Biochemical

scholarly journals Overexpression of EcbHLH57 Transcription Factor from Eleusine coracana L. in Tobacco Confers Tolerance to Salt, Oxidative and Drought Stress

PLoS ONE ◽  
2015 ◽  
Vol 10 (9) ◽  
pp. e0137098 ◽  
Author(s):  
K. C. Babitha ◽  
Ramu S. Vemanna ◽  
Karaba N. Nataraja ◽  
M. Udayakumar
Keyword(s):  
Transcription Factor ◽  
Drought Stress ◽  
Eleusine Coracana

scholarly journals Effect of Rhizophagus intraradices on growth and physiological performance of Finger Millet (Eleusine coracana L.) under drought stress

2021 ◽  
Vol 8 (4) ◽  
Author(s):  
Jaagriti Tyagi ◽  
Neeraj Shrivastava ◽  
AK Sharma ◽  
Ajit Varma ◽  
Ramesh Pudake
Keyword(s):  
Drought Stress ◽  
Finger Millet ◽  
Soluble Sugars ◽  
Leaf Tissue ◽  
Am Fungi ◽  
Eleusine Coracana ◽  
Mycorrhizal Symbiosis ◽  
Severe Drought ◽  
Rhizophagus Intraradices

Under abiotic stress conditions, arbuscular mycorrhizal (AM) fungi help plants by improving nutrient and water uptake. Finger millet (Eleusine coracana L.) is an arid crop having soils with poor water holding capacity. Therefore, it is difficult for the plants to obtain water and mineral nutrients from such soil to sustain life. To understand the role of mycorrhizal symbiosis in water and mineral up-take from the soil, we studied the role of Rhizophagus intraradices colonization and its beneficial role for drought stress tolerance in finger millet seedlings. Under severe drought stress condition, AM inoculation led to the significant increase in plant growth (7 %), phosphorus and chlorophyll content (29 %). Also, under drought stress the level of osmolytes such as proline and soluble sugars were found to be increased in AM inoculated seedlings. Under water stress, the lipid peroxidation in leaves of mycorrhized seedlings was reduced by 29 %. The flavonoid content of roots in AM colonized seedlings was found 16 % higher compared to the control, whereas the leaves were accumulated more phenol. Compared to the control, ascorbate level was found to be 25 % higher in leaf tissue of AM inoculated seedlings. Moreover, glutathione (GSH) level was also increased in mycorrhiza inoculated seedlings with a maximum increment of 182 % under severe stress. The results demonstrated that AM provided drought tolerance to the finger millet seedlings through a stronger root system, greater photosynthetic efficiency, a more efficient antioxidant system and improved osmoregulation.

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