scholarly journals Antibacterial effect of silver diamine fluoride and potassium iodide against E. faecalis, A. naeslundii and P. micra

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Benjamín Briseño-Marroquín ◽  
Yasmine Ismael ◽  
Angelika Callaway ◽  
Christian Tennert ◽  
Thomas Gerhard Wolf
Keyword(s):  
Potassium Iodide ◽  
Root Canal ◽  
Bacterial Species ◽  
In Vitro Study ◽  
Bactericidal Effect ◽  
Silver Diamine Fluoride ◽  
Actinomyces Naeslundii ◽  
Root Canal Irrigants ◽  
Parvimonas Micra

Abstract Background The aim of this study was to determine in vitro the bactericidal potential of 38% silver diamine fluoride (SDF) alone, potassium iodide (PI) alone, and the two in combination (SDF + PI) against three bacterial species commonly found in root canal samples (Enterococcus faecalis, Actinomyces naeslundii and Parvimonas micra). Methods The potential bactericidal rates for SDF, PI and SDF + PI against E. faecalis, A. naeslundii and P. micra were calculated as reduction of bacteria colony forming units. Results The bactericidal potential of SDF was at 99.97–100% against E. faecalis and 100% against A. naeslundii and P. micra. SDF + PI showed a 100% bactericidal effect against P. micra, 99.89–99.98% against E. faecalis and 99.98–100% against A. naeslundii. The bactericidal effect of PI was 99.51–99.98% against E. faecalis, 99.27–99.95% against A. naeslundii and 99.93–100% against P. micra. The differences between controls and bacteria exposed to the antibacterial agents were statistically significant (p < 0.05). Conclusions SDF had an effective bactericidal effect against the examined bacteria. However, the limitations of this in vitro study do not allow a recommendation of the employment of these solutions as root canal irrigants. Additional investigations are necessary to assess their endodontic clinical applicability.

scholarly journals UJI SITOTOKSISITAS EKSTRAK DAUN MIMBA (Azadirachta indica) TERHADAP SEL FIBROBLAS BHK 21

2017 ◽  
Vol 7 (1) ◽  
pp. 48
Author(s):  
Qurni Restiani ◽  
Mandojo Rukmo ◽  
Devi Eka Juniarti
Keyword(s):  
Antibacterial Activity ◽  
Optical Density ◽  
Azadirachta Indica ◽  
Root Canal ◽  
In Vitro Study ◽  
Cytotoxicity Test ◽  
Root Canal Irrigants ◽  
Neem Leaves ◽  
Alternative Material

Background: The leaves of neem (Azadirachta indicia) is one of herbal medicine that recommended as an alternative material of root canal irrigants. The active ingredients of neem leaves such as alkaloids, tannins, saponins and flavonoids has been proven to have antibacterial activity against E. faecalis. The ideal properties of an alternative material of root canal irrigants is not only have antibacterial activity but also is not toxic to the tissues, but the toxicity of neem leaves remains unclear until now. Objective: The aims of this study to determine the toxicity effect of neem leaves extract in specific concentration. Methods: This research was an experimental laboratory in vitro study of baby hamster kidney fibroblast (BHK-21). The neem leaves extract was made by maceration method using ethanol 96% and certain dilution performed to obtain various concentrations. Cytotoxicity test was conducted by MTT assay and the optical density was measured using ELISA reader at wavelength of 620 nm. Then, the optical density values were calculated using the formula for determining the number of survival fibroblasts after tested. Results: The percentage of survival fibroblast at concentration of 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95, and 100% in sequence were 71.3%, 64, 2%, 62%, 60.2%, 40.1%, 36.3%, 36.7%, 29%, 28.4%, 27.5%, and 25.6% . Conclusion: The extract of neem leaves (Azadirachta indica) has cytotoxic effects at concentration of 70% up to 100%.

scholarly journals Orange-Brown Precipitate: A Threat to the Success of Endodontic Treatment – An In Vitro Study

2020 ◽  
Vol 8 (D) ◽  
pp. 53-57
Author(s):  
Shashidhar Chandrashekhar ◽  
Dinesh Rao ◽  
Jyothi Shashidhar ◽  
Smitha Naik
Keyword(s):  
Root Canal ◽  
Isopropyl Alcohol ◽  
Sodium Thiosulfate ◽  
In Vitro Study ◽  
Control Group ◽  
Group 4 ◽  
Root Canal Irrigants ◽  
Group 5 ◽  
Brown Precipitate

AIM: The aim of the present study was to evaluate the effect of various intermediate irrigating solutions in the removal of orange-brown precipitate formed due to alternative use of sodium hypochlorite (NaOCl) and chlorhexidine (CHX) root canal irrigants. METHODS: The root canals of 50 mandibular premolars were prepared using ProTaper Universal Rotary instruments (Dentsply Maillefer, Ballaigues, Switzerland) up to size F3. The roots were randomly divided into five experimental groups specified by the intermediate irrigant used; Group 1: No intermediate irrigant used (control), Group 2: Saline, Group 3: 7% maleic acid (MA), Group 4: 4% sodium thiosulfate, and Group 5: 70% isopropyl alcohol (n = 10). After final irrigation with CHX, the specimens were evaluated for the presence/absence of orange-brown precipitate under stereomicroscope. RESULTS: Mean precipitate for all experimental groups was calculated using ANOVA F test which showed orange-brown precipitate (parachloroanaline) in all groups except Group 4 and Group 5 which were highly significant (p < 0.001) at all the levels of root canal. Intergroup pairwise comparison was done using Tukey’s post hoc test. CONCLUSION: About 7% MA is effective in minimizing the formation of orange-brown precipitate when used as intermediate irrigant between NaOCl and CHX. However, the precipitate was not observed with 4% sodium thiosulfate and 70% isopropyl alcohol.

scholarly journals Antimicrobial activity of root canal irrigants against biofilm forming pathogens- An in vitro study

2017 ◽  
Vol 20 (3) ◽  
pp. 147 ◽  
Author(s):  
SheetalBasavraj Ghivari ◽  
Haimanti Bhattacharya ◽  
KishoreG Bhat ◽  
MadhuA Pujar
Keyword(s):  
Antimicrobial Activity ◽  
Root Canal ◽  
In Vitro Study ◽  
Vitro Study ◽  
Root Canal Irrigants

scholarly journals Effect of different root canal irrigants on the sealing ability of two all-in-one self-etch adhesives: An in vitro study

2012 ◽  
Vol 15 (4) ◽  
pp. 377 ◽  
Author(s):  
Rajni Nagpal ◽  
ChandrakarChaman Mishra ◽  
UdaiPratap Singh ◽  
Rashi Agrawal ◽  
ShashiPrabha Tyagi
Keyword(s):  
Root Canal ◽  
In Vitro Study ◽  
Vitro Study ◽  
Root Canal Irrigants ◽  
Sealing Ability ◽  
Self Etch

scholarly journals Root canal irrigants influence the hydrophobicity and adherence of Staphylococcus epidermidis to root canal dentin: an in vitro study

2018 ◽  
Vol 43 (1) ◽  
Author(s):  
Venkateshbabu Nagendrababu ◽  
Omer Sheriff Sultan ◽  
Sreedharan Kannathasan ◽  
Amir Shahreza Patel ◽  
Ebenezer Chitra ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Root Canal ◽  
In Vitro Study ◽  
Vitro Study ◽  
Root Canal Irrigants ◽  
Root Canal Dentin

scholarly journals The Antibacterial Effects of Quaternary Ammonium Salts in the Simulated Presence of Inhibitors in Root Canals: A Preliminary In-Vitro Study

Coatings ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 181 ◽  
Author(s):  
Sanjay Kumar Tiwari ◽  
Suping Wang ◽  
Yannan Huang ◽  
Xuedong Zhou ◽  
Hockin H. K. Xu ◽  
...  
Keyword(s):  
Quaternary Ammonium ◽  
Root Canal ◽  
Bacterial Species ◽  
In Vitro Study ◽  
Quaternary Ammonium Salts ◽  
Ammonium Salts ◽  
Antibacterial Effects ◽  
Dentinal Tubules ◽  
Dentine Surface

To investigate the antibacterial effects of two newly developed quaternary ammonium salts (QAMs)-dimethylaminododecyl methacrylate (DMADDM) and dimethylaminohexadecyl methacrylate (DMAHDM), in the presence of various root canal inhibitors. Streptococcus gordonii, Enterococcus faecalis, Lactobacillus acidophilus and Actinomyces naeslundii were used. Dentine, dentine matrix and dead bacteria were selected as inhibitors. The antimicrobial efficacy of monomers of DMADDM and DMAHDM was tested against suspensions formed by mixtures of four bacterial species in or without the presence of inhibitors. The inhibition results were compared with chlorhexidine (CHX) and sodium hypochlorite (NaOCl). One-way analyses of variance (ANOVA) followed by Tukey’s multiple comparison test was performed to determine significant differences. The antibacterial effects of DMADDM and DMAHDM were variably inhibited dentine, dentine matrix and dead bacteria. CHX and NaOCl showed substantivity and they inhibited bacteria present in suspension. The concentration of compound decreased in the dentine block due to constant release. Bacterial colonies on the dentine surface and dentine tubules were significantly inhibited by DMADDM and DMAHDM. The antibacterial effects of DMADDM and DMAHDM could be inactivated by dentine, dentine matrix and dead bacteria, while DMADDM and DMAHDM could inhibit bacteria colonization on the dentine surface and kill bacteria present in dentinal tubules. The antibacterial effects of DMADDM and DMAHDM as free monomers in the presence of inhibitors was evaluated for the first time. They could help inhibit the residual bacteria on the dentine surface and in dentinal tubules that may cause persisting infection. Therefore the novel QAMs showed great potentials as root canal medication.

Bactericidal effect of the CO 2 laser in the root canal: an in-vitro study

1998 ◽  
Author(s):  
Norbert Gutknecht ◽  
Cornelia Wilkert-Walter ◽  
Friedrich Lampert
Keyword(s):  
Root Canal ◽  
In Vitro Study ◽  
Bactericidal Effect ◽  
Vitro Study
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