Co-prevalence of human Papillomaviruses (HPV) and Epstein–Barr virus (EBV) in healthy blood donors from diverse nationalities in Qatar

Introduction: Infections with human oncoviruses such as high-risk human papillomaviruses (HPVs) and Epstein-Barr virus (EBV) are globally prevalent in the adult population. Both viruses are strongly associated with several types of human carcinomas such as cervical, head and neck, nasopharyngeal and gastric. In the present study, we explored the prevalence of these two oncoviruses in the healthy population of Qatar. Methods: The study included 385 healthy blood donors that reflect diverse nationalities in the Qatari community (Qatar, Egypt, Syria, Jordan, Pakistan, and India). DNA was extracted from the peripheral blood and genotyping was done using PCR and nested-PCR targeting E6 and E7 as well as LMP1 genes of HPVs and EBV, respectively. Results: The age of participants (378 males and 7 females) ranged between 19 and 68 years (mean 37.12 ± 9.3 years). Our data indicate that 55% and 61% of the tested samples were HPVs and EBV positive, respectively. Moreover, we found that there was (40%) co-presence of both HPVs and EBV in our samples. The most common high-risk HPV types in Qatar included HPV 59 (55%), 31 (54%), 52 (49%), 51 (49%), 58 (47%) and 35 (46%). While, HPV 16 and 18 were detected in 38% and 36% of the samples, respectively. Notably, all samples showed multiple HPVs infections. Conclusion: Our study reveals for the first time a high prevalence of both EBV and HPVs among healthy individuals in Qatar. More significantly, most cases had multiple HPV types infection in addition to the co-presence of both viruses in a substantial proportion of the samples. Given the important possible cooperative role of these viruses in human carcinogenesis, preventive measures using available and upcoming vaccines are of paramount importance.

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Comparison of Co-Presence of Epstein-Barr Virus and High-Risk Human Papillomaviruses in Colorectal Cancers in the Middle East Region

University of the Future: Re-Imagining Research and Higher Education ◽

10.29117/quarfe.2020.0121 ◽

2020 ◽

Author(s):

Karim Nagi ◽

Ishita Gupta ◽

Hamda A Al-Thawadi ◽

Ayesha Jabeen ◽

Mohammed I. Malk ◽

...

Keyword(s):

Colorectal Cancer ◽

High Risk ◽

Epstein Barr Virus ◽

Human Papillomaviruses ◽

Colorectal Cancers ◽

Barr Virus ◽

Human Carcinomas ◽

Epstein Barr ◽

Invasive Carcinomas ◽

High Risk Hpv

Background: Several studies have shown the presence of onco viral DNA in colorectal tumor tissues. Viral infection by onco-viruses such as Human papillomaviruses (HPVs) and Epstein–Barr virus (EBV) are well-known to be involved in the onset and/or progression of numerous human carcinomas. Methods: We explored the co-presence of high-risk HPVs and EBV in a cohort of colorectal cancer samples from Lebanon (94) and Syria (102) by PCR, immunohistochemistry and tissue microarray. Results: The results of the study point out that 54% of colorectal cancer cases in Syria are positive for high-risk HPVs, while 30% of the cases in Lebanon are positive for these viruses; the most frequent high-risk HPV types in these populations are 16, 18, 31, 33 and 35. Analysis of LMP1 showed similar results in both populations; 36% of Syrian and 31% of Lebanese samples. Additionally, we report that EBV and high-risk HPVs are co-present in these samples. In Syrian samples, EBV and HPVs are co-present in 16% of the population, however, in the Lebanese samples, 20% of the cases are positive for both EBV and HPVs; their co-presence is associated with high/intermediate grade invasive carcinomas. Conclusion: These data suggest that EBV and high-risk HPVs are co-present in human colorectal cancers where they can cooperate in the progression of these cancers. Nevertheless, further studies are needed to elucidate the role of those oncoviruses in the development of human colorectal carcinomas.

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Simultaneous detection of Epstein - Barr virus and Cytomegalovirus DNA in Mini-pooling of Whole blood Samples from Iraqi blood donors by Multiplex Real-Time PCR Assay

10.36295/asro.2020.231441 ◽

2020 ◽

Vol 23 (14) ◽

Author(s):

Ghinwa S. Majid ◽

Ahmed S. Abdulamir ◽

Abbas M. Ahmed ◽

Iman M. Aufi ◽

Orooba I. Abdullah

Keyword(s):

Real Time Pcr ◽

Whole Blood ◽

Epstein Barr Virus ◽

Blood Donors ◽

Simultaneous Detection ◽

Pcr Assay ◽

Blood Samples ◽

Barr Virus ◽

Whole Blood Samples ◽

Epstein Barr

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Co-Incidence of Human Papillomaviruses and Epstein–Barr Virus is Associated with Advanced Tumor Stage: A Tissue Microarray Study of Head and Neck Cancers

University of the Future: Re-Imagining Research and Higher Education ◽

10.29117/quarfe.2020.0122 ◽

2020 ◽

Author(s):

Ishita Gupta ◽

Hamda A. Al-Thawadi ◽

Faruk Skenderi ◽

Mohammed I. Malki ◽

Semir Vranic ◽

...

Keyword(s):

Head And Neck ◽

Tissue Microarray ◽

Epstein Barr Virus ◽

Tumor Stage ◽

Human Papillomaviruses ◽

Advanced Tumor Stage ◽

Barr Virus ◽

Microarray Study ◽

Advanced Tumor ◽

Epstein Barr

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Epstein-Barr virus transmission from a blood donor to an organ transplant recipient with recovery of the same virus strain from the recipient's blood and oropharynx

Blood ◽

10.1182/blood.v87.2.812.bloodjournal872812 ◽

1996 ◽

Vol 87 (2) ◽

pp. 812-817 ◽

Cited By ~ 57

Author(s):

C Alfieri ◽

J Tanner ◽

L Carpentier ◽

C Perpete ◽

A Savoie ◽

...

Keyword(s):

Cord Blood ◽

Blood Donor ◽

Epstein Barr Virus ◽

Blood Donors ◽

Barr Virus ◽

Ebv Infection ◽

Transplant Patients ◽

Throat Wash ◽

Transforming Activity ◽

Epstein Barr

A previous study (Savoie et al, Blood 83:2715, 1994) identified eight transplant patients who acquired Epstein-Barr virus (EBV) infection during the peritransplant period. Three of these patients subsequently developed B-cell lymphoproliferative disease within 4 months of transplantation. Among these, there was a 16-year-old liver transplant patient who was negative for EBV at the time of transplant and who received an EBV-negative organ. After transplant, this patient was transfused with 9 U of packed red blood cells. Eight of the donors were EBV-positive and one was EBV-negative. We succeeded in obtaining spontaneous lymphoblastoid cell lines (LCLs) from the blood of three of these donors, one of whom also yielded a cord-blood line established with his throat-wash EBV. Blood from a fourth donor did not yield an LCL, but his throat washing did have transforming activity when inoculated onto cord-blood leukocytes. We initially could establish spontaneous LCLs only from the recipient's blood. However, a throat- wash sample taken 11 weeks later did show transforming activity. The recipient was shown to have acquired the EBV infection from one of eight EBV-seropositive blood donors. Analysis of fragment length polymorphisms after polymerase chain reaction amplification of the EBV BamHI-K fragment was used to establish strain identity. Western blot analysis for existence of size polymorphisms in three classes of Epstein-Barr nuclear antigens (EBNA-1, EBNA-2, and EBNA-3) confirmed the DNA results. It is noteworthy that the blood donor responsible for transmitting his EBV strain to the recipient had experienced clinical infectious mononucleosis 15 months before donating blood. Our results may, thus, indicate a requirement for leukodepletion of blood destined for immunosuppressed EBV-negative patients. Finally, blood donors with a recent history of infectious mononucleosis should probably be identified so that their blood is not given to EBV-negative transplant patients.

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Molecular diagnosis of cytomegalovirus, Epstein-Barr virus and Herpes virus 6 among blood donors in Ouagadougou, Burkina Faso

BMC Infectious Diseases ◽

10.1186/1471-2334-14-s2-p99 ◽

2014 ◽

Vol 14 (S2) ◽

Cited By ~ 1

Author(s):

Lassina Traore ◽

Issoufou Tao ◽

Cyrille Bisseye ◽

Florencia Djigma ◽

Djénéba Ouermi ◽

...

Keyword(s):

Burkina Faso ◽

Molecular Diagnosis ◽

Epstein Barr Virus ◽

Herpes Virus ◽

Blood Donors ◽

Barr Virus ◽

Epstein Barr ◽

Herpès Virus

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Polymorphism of the IL-1 Gene Complex in Epstein-Barr Virus Seronegative and Seropositive Adult Blood Donors

Scandinavian Journal of Immunology ◽

10.1046/j.1365-3083.1998.00406.x ◽

1998 ◽

Vol 48 (3) ◽

pp. 219-222 ◽

Cited By ~ 29

Author(s):

Hurme ◽

Helminen

Keyword(s):

Epstein Barr Virus ◽

Blood Donors ◽

Gene Complex ◽

Barr Virus ◽

Epstein Barr

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Investigation of human papillomaviruses (HPV), mouse mammary tumour virus (MMTV), Epstein–Barr virus (EBV), and human polyomavirus entities in canine mammary tumours

Journal of Veterinary Research ◽

10.1515/jvetres-2016-0008 ◽

2016 ◽

Vol 60 (1) ◽

pp. 49-53 ◽

Cited By ~ 2

Author(s):

Kivilcim Sonmez ◽

Eda Altan ◽

Funda Yildirim ◽

Seçkin Serdar Arun ◽

Nuri Turan ◽

...

Keyword(s):

Epstein Barr Virus ◽

Human Papillomaviruses ◽

Human Polyomavirus ◽

Mammary Tumour ◽

Mammary Tissue ◽

Pcr Analysis ◽

Mouse Mammary Tumour Virus ◽

Barr Virus ◽

Mammary Tumours ◽

Epstein Barr

Abstract Introduction: The aim of the study was to investigate the presence of human papillomaviruses (HPV), mouse mammary tumour virus (MMTV), Epstein-Barr virus (EBV), and human polyomavirus BK in canine mammary tumours (CMTs) and to correlate the results of histopathological classification with the results of virological examination. Material and Methods: Eighty CMTs and ten normal canine mammary gland samples were evaluated using histopathological methods and TaqMan real-time PCR analysis. Results: The results indicated that all mammary tumours and normal mammary tissue samples were negative for HPV16 and other HPV, EBV, human polyomavirus, and human mammary tumour virus strains. Conclusion: Further studies should be performed to investigate the existence of other strains of HPV, EBV, and human polyomavirus in CMTs.

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