scholarly journals Spatio-temporal and -spectral feature maps in photoplethysmography imaging and infrared thermograph

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Michael Paul ◽  
Sabrina Caprice Behr ◽  
Christoph Weiss ◽  
Konrad Heimann ◽  
Thorsten Orlikowsky ◽  
...  

Abstract Background Only a small fraction of the information available is generally used in the majority of camera-based sensing approaches for vital sign monitoring. Dedicated skin pixels, for example, fall into this category while other regions are often disregarded early in the processing chain. Methods We look at a simple processing chain for imaging where a video stream is converted to several other streams to investigate whether other image regions should also be considered. These streams are generated by mapping spatio-temporal and -spectral features of video segments and, thus, compressing the information contained in several seconds of video and encoding these in a new image. Two typical scenarios are provided as examples to study the applicability of these maps: face videos in a laboratory setting and measurements of a baby in the neonatal intensive care unit. Each measurement consists of the synchronous recording of photoplethysmography imaging (PPGI) and infrared thermography (IRT). We report the results of a visual inspection of those maps, evaluate the root mean square (RMS) contrast of foreground and background regions, and use histogram intersections as a tool for similarity measurements. Results The maps allow us to distinguish visually between pulsatile foreground objects and an image background, which is found to be a noisy pattern. Distortions in the maps could be localized and the origin could be discovered. The IRT highlights subject contours for the heart frequency band, while silhouettes show strong signals in PPGI. Reflections and shadows were found to be sources of signals and distortions. We can testify advantages for the use of near-infrared light for PPGI. Furthermore, a difference in RMS contrast for pulsatile and non-pulsatile regions could be demonstrated. Histogram intersections allowed us to differentiate between the background and foreground. Conclusions We introduced new maps for the two sensing modalities and presented an overview for three different wavelength ranges. The maps can be used as a tool for visualizing aspects of the dynamic information hidden in video streams without automation. We propose focusing on an indirect method to detect pulsatile regions by using the noisy background pattern characteristic, for example, based on the histogram approach introduced.

2020 ◽  
Vol 48 (6) ◽  
pp. 2657-2667
Author(s):  
Felipe Montecinos-Franjola ◽  
John Y. Lin ◽  
Erik A. Rodriguez

Noninvasive fluorescent imaging requires far-red and near-infrared fluorescent proteins for deeper imaging. Near-infrared light penetrates biological tissue with blood vessels due to low absorbance, scattering, and reflection of light and has a greater signal-to-noise due to less autofluorescence. Far-red and near-infrared fluorescent proteins absorb light >600 nm to expand the color palette for imaging multiple biosensors and noninvasive in vivo imaging. The ideal fluorescent proteins are bright, photobleach minimally, express well in the desired cells, do not oligomerize, and generate or incorporate exogenous fluorophores efficiently. Coral-derived red fluorescent proteins require oxygen for fluorophore formation and release two hydrogen peroxide molecules. New fluorescent proteins based on phytochrome and phycobiliproteins use biliverdin IXα as fluorophores, do not require oxygen for maturation to image anaerobic organisms and tumor core, and do not generate hydrogen peroxide. The small Ultra-Red Fluorescent Protein (smURFP) was evolved from a cyanobacterial phycobiliprotein to covalently attach biliverdin as an exogenous fluorophore. The small Ultra-Red Fluorescent Protein is biophysically as bright as the enhanced green fluorescent protein, is exceptionally photostable, used for biosensor development, and visible in living mice. Novel applications of smURFP include in vitro protein diagnostics with attomolar (10−18 M) sensitivity, encapsulation in viral particles, and fluorescent protein nanoparticles. However, the availability of biliverdin limits the fluorescence of biliverdin-attaching fluorescent proteins; hence, extra biliverdin is needed to enhance brightness. New methods for improved biliverdin bioavailability are necessary to develop improved bright far-red and near-infrared fluorescent proteins for noninvasive imaging in vivo.


2020 ◽  
Author(s):  
Alex Stafford ◽  
Dowon Ahn ◽  
Emily Raulerson ◽  
Kun-You Chung ◽  
Kaihong Sun ◽  
...  

Driving rapid polymerizations with visible to near-infrared (NIR) light will enable nascent technologies in the emerging fields of bio- and composite-printing. However, current photopolymerization strategies are limited by long reaction times, high light intensities, and/or large catalyst loadings. Improving efficiency remains elusive without a comprehensive, mechanistic evaluation of photocatalysis to better understand how composition relates to polymerization metrics. With this objective in mind, a series of methine- and aza-bridged boron dipyrromethene (BODIPY) derivatives were synthesized and systematically characterized to elucidate key structure-property relationships that facilitate efficient photopolymerization driven by visible to NIR light. For both BODIPY scaffolds, halogenation was shown as a general method to increase polymerization rate, quantitatively characterized using a custom real-time infrared spectroscopy setup. Furthermore, a combination of steady-state emission quenching experiments, electronic structure calculations, and ultrafast transient absorption revealed that efficient intersystem crossing to the lowest excited triplet state upon halogenation was a key mechanistic step to achieving rapid photopolymerization reactions. Unprecedented polymerization rates were achieved with extremely low light intensities (< 1 mW/cm<sup>2</sup>) and catalyst loadings (< 50 μM), exemplified by reaction completion within 60 seconds of irradiation using green, red, and NIR light-emitting diodes.


2020 ◽  
Vol 59 (11) ◽  
pp. 110906
Author(s):  
Juan Shen ◽  
Yong Ren ◽  
Xinxin Zhu ◽  
Min Mao ◽  
Quan Zhou ◽  
...  

Author(s):  
Xiaowei Luan ◽  
Yongchun Pan ◽  
Yanfeng Gao ◽  
Yujun Song

Light has witnessed the history of mankind and even the universe. It is of great significances to the life of human society, contributing to energy, agriculture, communication, and much more....


1998 ◽  
Vol 11 (1) ◽  
pp. 395-395
Author(s):  
S. Nishida ◽  
T. Tanabé ◽  
S. Matsumoto ◽  
T. Onaka ◽  
Y. Nakada ◽  
...  

A systematic near-infrared survey was made for globular clusters in the Magellanic Clouds. Two infrared stars were discovered in NGC419 (SMC) and NGC1783 (LMC). NGC419 and NGC1783 are well-studied rich globular clusters whose turn-off masses and ages are estimated MTO ~ 2.0 Mʘ and т ~1.2 Gyr for NGC419, and MT0 ~ 2.0 Mʘ and т ʘ 0.9 Gyr for NGC1783, respectively. The periods of the infrared light variations were determined to be 540 dfor NGC419IR1 and to be 480 d for NGC1783IR1, respectively. Comparison of the measurements with the period—if magnitude relation for carbon Miras in the LMC by Groenewegen and Whitelock(1996) revealed that the Kmagnitudes of the infrared stars were fainter by about 0.3 — 0.8 magnitude than those predicted by the P — K relation. This deviation can be explained if the infrared stars are surrounded by thick dust shells and are obscured even in the K band. The positions of NGC419IR1and NGC1783IR1 on the P — K diagram suggest that AGB stars with the main sequence masses of about 2 Mʘ start their heavy mass-loss when P ʘ 500 d.


2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Ibrahim Shaik ◽  
S. K. Begum ◽  
P. V. Nagamani ◽  
Narayan Kayet

AbstractThe study demonstrates a methodology for mapping various hematite ore classes based on their reflectance and absorption spectra, using Hyperion satellite imagery. Substantial validation is carried out, using the spectral feature fitting technique, with the field spectra measured over the Bailadila hill range in Chhattisgarh State in India. The results of the study showed a good correlation between the concentration of iron oxide with the depth of the near-infrared absorption feature (R2 = 0.843) and the width of the near-infrared absorption feature (R2 = 0.812) through different empirical models, with a root-mean-square error (RMSE) between < 0.317 and < 0.409. The overall accuracy of the study is 88.2% with a Kappa coefficient value of 0.81. Geochemical analysis and X-ray fluorescence (XRF) of field ore samples are performed to ensure different classes of hematite ore minerals. Results showed a high content of Fe > 60 wt% in most of the hematite ore samples, except banded hematite quartzite (BHQ) (< 47 wt%).


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