scholarly journals Prevalence of tetracycline resistance genes among multi-drug resistant bacteria from selected water distribution systems in southwestern Nigeria

2015 ◽  
Vol 14 (1) ◽  
Author(s):  
Ayodele. T. Adesoji ◽  
Adeniyi. A. Ogunjobi ◽  
Isaac. O. Olatoye ◽  
Douglas. R. Douglas
Keyword(s):  
Resistance Genes ◽  
Distribution Systems ◽  
Water Distribution ◽  
Water Distribution Systems ◽  
Tetracycline Resistance ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Southwestern Nigeria ◽  
Tetracycline Resistance Genes ◽  
Drug Resistant Bacteria

scholarly journals Identification of Aminoglycoside Resistance Genes From Bacteria Isolated From Selected Municipal Drinking Water Distribution Systems in Southwestern Nigeria

2020 ◽  
Vol 41 (S1) ◽  
pp. s255-s255
Author(s):  
Ayodele T. Adesoji ◽  
Adeniyi A. Ogunjobi
Keyword(s):  
Drinking Water ◽  
Distribution Systems ◽  
Water Distribution ◽  
Water Distribution Systems ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Multidrug Resistant Bacteria ◽  
Southwestern Nigeria ◽  
Drinking Water Distribution Systems ◽  
Drinking Water Distribution

Background: Multidrug-resistant bacteria can lead to treatment failure, resulting in infectious diseases being transferred through nonpotable water. Aminoglycosides are an important class of antibiotics that are abused in Nigeria. Few studies have investigated aminoglycoside-modifying genes (AMGs) that are likely responsible for resistance in Nigeria bacteria isolates. Therefore, we aimed to characterize AMGs from isolates in drinking water distribution systems (DWDS) in southwestern Nigeria. Methods: Multidrug-resistant bacteria (n = 181) that had been previously characterized by 16S rDNA sequencing and that were positive for resistance to at least 1 aminoglycoside antibiotic were selected from 6 treated and untreated water distribution systems. Strains were PCR genotyped for 3 AMGs: aph(3)c, ant(3)b and aph(6)-1dd. Results: Of 181 MDR bacteria tested, 69 (38.12%) were positive for at least 1 of the AMGs. The most common was ant(3)c (27.6%), followed by aph(3")c (18.23%). Both aph(3)c and ant(3")b were found in 7.73% of tested isolates, ant(3)b was most commonly found in Alcaligenes spp (50%). Furthermore, aph(3")c was most commonly detected in Proteus spp (50%). Other genera positive for AMGs included Acinetobacter, Aeromonas, Bordetella, Brevundimonas, Chromobacterium, Klebsiella, Leucobacter, Morganella, Pantoae, Proteus, Providencia, Psychrobacter, and Serratia. Conclusions: High occurrence of ant(3)c and aph(3)c among these bacteria call for urgent attention among public health workers because these genes can be easily disseminated to consumers if present on mobile genetic elements like plasmids, integrons, and transposons.Funding: NoneDisclosures: None

scholarly journals Genotypic Characterization of Aminoglycoside Resistance Genes from Bacteria Isolates in Selected Municipal Drinking Water Distribution Sources in Southwestern Nigeria

1970 ◽  
Vol 29 (3) ◽  
Author(s):  
Adesoji. A. Timilehin ◽  
Olatoye. I. Olufemi ◽  
Ogunjobi. A. Adeniyi
Keyword(s):  
Drinking Water ◽  
Resistance Genes ◽  
Distribution Systems ◽  
Water Distribution ◽  
Health Workers ◽  
Water Distribution Systems ◽  
Aminoglycoside Resistance ◽  
Southwestern Nigeria ◽  
Drinking Water Distribution

BACKGROUND: Multi-drug Resistant (MDR) bacteria could lead to treatment failure of infectious diseases and could be transferred by non-potable water. Few studies have investigated occurrence of Antibiotic Resistance Genes (ARGs) among bacteria including Aminoglycoside Modifying Genes (AMGs) from Drinking Water Distribution Systems (DWDS) in Nigeria. Here, we aimed at characterization of AMGs from DWDS from selected states insouthwestern Nigeria.METHODS: One hundred and eighty one (181) MDR bacteria that had been previously characterized using 16S rDNA and showed resistance to at least one aminoglycoside antibiotic were selected from treated and untreated six water distribution systems in southwestern Nigeria. MDR bacteria were PCR genotyped for three AMGs:aph (3´´)c, ant (3´´)b and aph(6)-1dd.RESULTS: Out of 181 MDR bacteria genotyped, 69(38.12%) tested positive for at least one of the genotyped AMGs. Highest (50, 27.62%) detected gene was ant (3”)c followed by aph (3")c(33,18.23%). Combination of aph(3")c and ant (3")b in a single bacteria was observed as the highest (14, 7.73%) among the detected gene combination. Alcaligenes sp showed the highest (10/20) occurrence of ant (3")b while aph(3")c was the highest detected among Proteussp (11/22). Other bacteria that showed the presence of AMGs include: Acinetobacter, Aeromonas, Bordetella, Brevundimonas, Chromobacterium, Klebsiella, Leucobacter, Morganella, Pantoae, Proteus, Providencia, Psychrobacter and Serratia.CONCLUSIONS: High occurrence of ant (3”)c and aph (3”)c among these bacteria call for urgent attention among public health workers, because these genes can be easily disseminated to consumers of these water samples if present on mobile genetic elements like plasmids, integrons and transposons.

scholarly journals Genome-Resolved Metagenomics and Antibiotic Resistance Genes Analysis in Reclaimed Water Distribution Systems

Water ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 3477
Author(s):  
Changzhi Wang ◽  
Pei-Ying Hong
Keyword(s):  
Antibiotic Resistance ◽  
Relative Abundance ◽  
Resistance Genes ◽  
Distribution Systems ◽  
Water Distribution ◽  
Reclaimed Water ◽  
Water Distribution Systems ◽  
Antibiotic Resistance Genes ◽  
Quality Data ◽  
Metagenomic Data

Water reuse is increasingly pursued to alleviate global water scarcity. However, the wastewater treatment process does not achieve full removal of biological contaminants from wastewater, hence microorganisms and their genetic elements can be disseminated into the reclaimed water distribution systems (RWDS). In this study, reclaimed water samples are investigated via metagenomics to assess their bacterial diversity, metagenome-assembled genomes (MAGs) and antibiotic resistance genes (ARGs) at both point of entry (POE) and point of use (POU) in 3 RWDS. The number of shared bacterial orders identified by metagenome was higher at the POE than POU among the three sites, indicating that specific conditions in RWDS can cause further differentiation in the microbial communities at the end of the distribution system. Two bacterial orders, namely Rhizobiales and Sphingomonadales, had high replication rates in two of the examined RWDS (i.e., site A and B), and were present in higher relative abundance in POU than at POE. In addition, MAG and ARG relative abundance exhibited a strong correlation (R2 = 0.58) in POU, indicating that bacteria present in POU may have a high incidence of ARG. Specifically, resistance genes associated with efflux pump mechanisms (e.g., adeF and qacH) increased in its relative abundance from POU to POE at two of the RWDS (i.e., site A and B). When correlated with the water quality data that suggests a significantly lower dissolved organic carbon (DOC) concentration at site D than the other two RWDS, the metagenomic data suggest that low DOC is needed to maintain the biological stability of reclaimed water along the distribution network.

scholarly journals Degradation Sulfamethoxazole by Chlorination in Water Distribution Systems: Kinetics, Toxicity, and Antibiotic Resistance Genes

2021 ◽  
Author(s):  
Luo Xu ◽  
cong li ◽  
Guozijian Wei ◽  
Jie Ji ◽  
Eric Lichtfouse ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Distribution Systems ◽  
Water Distribution ◽  
Degradation Rate ◽  
Tap Water ◽  
Water Distribution Systems ◽  
Antibiotic Resistance Genes ◽  
Feed Additives ◽  
Batch Reactors

Abstract Sulfamethoxazole (SMX) is a veterinary drugs and feed additives, which has been frequently detected in surface waters in recent years. This paper investigated the kinetics, evolution of toxicity and antibiotic resistance genes (ARGs) of SMX in reactions with free chlorine (FC) to evaluate the fate of SMX in batch reactors and water distribution systems (WDS). In the range of investigated pH (6.3 – 9.0), the SMX degradation had the fastest rate at close to neutral pH. The chlorination of SMX could be described by the first-order kinetics, with specific second-order rate constants in batch reactors of (2.23 ± 0.07) × 102 M− s and (5.04 ± 0.30) × 101 M− s− for HClO and ClO−, respectively. And in WDS of (1.76 ± 0.07) × 102 M− s− and (4.06 ± 0.62) × 101 M− s−, respectively. The SMX degradation rate was also affected by pipe material, and the rate followed the order: stainless-steel pipe (SS) > ductile iron pipe (DI) > polyethylene pipe (PE). The flow rate from 0.7 to 1.5 m/s led to an increase of SMX degradation rate in DI, but the increase was limited. In addition, SMX could increase the toxicity of water initially, yet the toxicity reduced to the level of tap water after 2 hours chlorination. The relative abundance of ARGs (sul1 and sul2) of tap water samples was significantly increased under different conditions including only use SMX, chlorination products of SMX, or pretreatment with SMX followed by chlorination.

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