scholarly journals Advances in mesenchymal stem cell conditioned medium-mediated periodontal tissue regeneration

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Hongbing Lin ◽  
Huishan Chen ◽  
Xuetao Zhao ◽  
Zhen Chen ◽  
Peipei Zhang ◽  
...  
Keyword(s):  
Conditioned Medium ◽  
Tissue Regeneration ◽  
Clinical Studies ◽  
Therapeutic Potential ◽  
Periodontal Regeneration ◽  
Periodontal Tissue ◽  
Periodontal Tissues ◽  
Periodontal Tissue Regeneration ◽  
Indirect Treatment ◽  
Direct Use

AbstractPeriodontitis is a chronic inflammatory disease that leads to the destruction of both soft and hard periodontal tissues. Complete periodontal regeneration in clinics using the currently available treatment approaches is still a challenge. Mesenchymal stem cells (MSCs) have shown promising potential to regenerate periodontal tissue in various preclinical and clinical studies. The poor survival rate of MSCs during in vivo transplantation and host immunogenic reaction towards MSCs are the main drawbacks of direct use of MSCs in periodontal tissue regeneration. Autologous MSCs have limited sources and possess patient morbidity during harvesting. Direct use of allogenic MSCs could induce host immune reaction. Therefore, the MSC-based indirect treatment approach could be beneficial for periodontal regeneration in clinics. MSC culture conditioned medium (CM) contains secretomes that had shown immunomodulatory and tissue regenerative potential in pre-clinical and clinical studies. MSC-CM contains a cocktail of growth factors, cytokines, chemokines, enzymes, and exosomes, extracellular vesicles, etc. MSC-CM-based indirect treatment has the potential to eliminate the drawbacks of direct use of MSCs for periodontal tissue regeneration. MSC-CM holds the tremendous potential of bench-to-bed translation in periodontal regeneration applications. This review focuses on the accumulating evidence indicating the therapeutic potential of the MSC-CM in periodontal regeneration-related pre-clinical and clinical studies. Recent advances on MSC-CM-based periodontal regeneration, existing challenges, and prospects are well summarized as guidance to improve the effectiveness of MSC-CM on periodontal regeneration in clinics.

Regeneration of Periodontal Tissues in Non-human Primates with rhGDF-5 and Beta-Tricalcium Phosphate

2011 ◽  
Vol 90 (12) ◽  
pp. 1416-1421 ◽  
Author(s):  
K.B. Emerton ◽  
S.J. Drapeau ◽  
H. Prasad ◽  
M. Rohrer ◽  
P. Roffe ◽  
...  
Keyword(s):  
Tissue Regeneration ◽  
Tricalcium Phosphate ◽  
Alveolar Bone ◽  
Periodontal Regeneration ◽  
Human Growth ◽  
Graft Material ◽  
Periodontal Tissue ◽  
Periodontal Tissues ◽  
Beta Tricalcium Phosphate ◽  
Periodontal Tissue Regeneration

The application of growth factors has been advocated in support of periodontal regeneration. Recombinant human growth and differentiation factor-5 (rhGDF-5), a member of the bone morphogenetic protein family, has been used to encourage periodontal tissue regeneration. This study evaluated the dose response of rhGDF-5 lyophilized onto beta-tricalcium phosphate (bTCP) granules for periodontal tissue regeneration in a baboon model. Periodontal defects were created bilaterally in 12 baboons by a split-mouth design. Plaque was allowed to accumulate around wire ligatures to create chronic disease. After 2 mos, the ligatures were removed, and a notch was placed at the base of the defect. Two teeth on each side of the mouth were randomly treated with bTCP only, 0.5, 1.0, or 2.0 mg rhGDF-5/g bTCP. Animals were sacrificed 5 mos post-treatment, with micro-CT and histomorphometric analysis performed. After 5 mos, analysis showed alveolar bone, cementum, and periodontal ligament formation in all treatment groups, with a dose-dependent increase in rhGDF-5-treated groups. Height of periodontal tissues also increased with the addition of rhGDF-5, and the amount of residual graft material decreased with rhGDF-5 treatment. Therefore, rhGDF-5 delivered on bTCP demonstrated effective regeneration of all 3 tissues critical for periodontal repair.

scholarly journals Local Injection of Allogeneic Stem Cells from Apical Papilla Enhanced Periodontal Tissue Regeneration in Minipig Model of Periodontitis

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Guoqing Li ◽  
Nannan Han ◽  
Xiuli Zhang ◽  
Haoqing Yang ◽  
Yangyang Cao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Tissue Regeneration ◽  
Alveolar Bone ◽  
Local Injection ◽  
Swine Model ◽  
Periodontal Tissue ◽  
Clinical Assessments ◽  
Periodontal Tissues ◽  
Periodontal Tissue Regeneration ◽  
Apical Papilla

Background. Discovering suitable seeding cells and simple application technique will be beneficial for MSC-mediated treatment of periodontitis. Stem cells from apical papilla (SCAPs) might be the candidate seeding cell for the periodontal tissues regeneration based on their origin and characters. In this research, we investigated the effect of SCAPs on periodontal tissue regeneration in swine by local injection. Methods. We established experimental periodontitis model in miniature pigs and then treated them with SCAPs by local injection. Clinical assessments, computed tomography (CT) scanning, histologic examination, and quantitative measurements were used to evaluate the effect of periodontal tissues regeneration. Results. At 12 weeks after injection, clinical assessments showed that probing depth, gingival recession, and attachment loss values were 5.44±0.77 mm versus 7.33±1.0 mm (p<0.01), 2.33±0.33 mm versus 2.11±0.69 mm (p>0.05), and 7.78±0.84 mm versus 9.44±1.07 mm (p<0.01) in SCAPs group and 0.9% NaCl group, respectively. CT scan results showed a significant increase of 12.86 mm3 alveolar bone regeneration in SCAPs group compared with 0.9% NaCl group. In addition, histopathology results demonstrated remarkable regeneration in SCAPs group, whereas regeneration of periodontal tissue was hardly found in 0.9% NaCl group. Conclusion. Local injection of SCAPs could effectively restore tissue defects brought about by periodontitis in the swine model. Thus, SCAPs, as an easily accessible dental-deriving stem cell, may serve as an alternative application for periodontitis treatment.

scholarly journals Effect of advanced platelet-rich fibrin applications on periodontal regeneration in infrabony pocket treatment

2019 ◽  
Vol 4 (3) ◽  
pp. 154
Author(s):  
Christopher Imantaka Suwondo ◽  
Dahlia Herawati ◽  
Sudibyo Sudibyo
Keyword(s):  
Tissue Regeneration ◽  
Alveolar Bone ◽  
Periodontal Regeneration ◽  
X Rays ◽  
Periodontal Tissue ◽  
Platelet Rich Fibrin ◽  
Attachment Loss ◽  
Probing Depth ◽  
Periodontal Tissue Regeneration ◽  
Bone Height

One of the regenerative periodontal treatments for infrabony pocket is open flap debridement (OFD) with the addition of growth factor derived from platelet concentrate. Advanced platelet-rich fibrin (A-PRF) is a further development of plateletrich fibrin (PRF) with a lower centrifugation speed (1,500 rpm, 14 minutes). The purpose of this study was to examine the differences in periodontal tissue regeneration after the application of A-PRF and PRF in the treatment of infrabony pockets evaluated from probing depth (PD), relative attachment loss (RAL), and alveolar bone height. The samples were taken from 20 infrabony pockets divided into 2 groups: 10 subjects were treated with OFD+A-PRF and OFD+PRF on the remaining subjects. Probing depth (PD) and relative attachment loss (RAL) measurement were performed on days 0, 30, and 90. Bone height measurements were performed using CBCT X-rays on days 0 and 90. The results showed that PD and RAL reduction in the group of OFD+A-PRF was significantly greater than that in the OFD+PRF group. Bone height reduction in both groups showed no difference. The conclusion obtained from this study is A-PRF application enhances periodontal tissue regeneration by generating greater probing depth and relative attachment loss reduction compared toPRF, as well as an increase in bone height similar to in the treatment of infrabony pockets.

Biodegradable engineered fiber scaffolds fabricated by electrospinning for periodontal tissue regeneration

2020 ◽  
pp. 088532822095225
Author(s):  
Xuanwen Xu ◽  
Shuangshuang Ren ◽  
Lu Li ◽  
Yi Zhou ◽  
Wenzao Peng ◽  
...  
Keyword(s):  
Tissue Regeneration ◽  
Technological Progress ◽  
Periodontal Diseases ◽  
Drug Loading ◽  
Periodontal Regeneration ◽  
Periodontal Tissue ◽  
Structure Adjustment ◽  
Periodontal Tissue Regeneration ◽  
Fiber Scaffolds ◽  
Research Situation

Considering the specificity of periodontium and the unique advantages of electrospinning, this technology has been used to fabricate biodegradable tissue engineering materials for functional periodontal regeneration. For better biomedical quality, a continuous technological progress of electrospinning has been performed. Based on property of materials (natural, synthetic or composites) and additive novel methods (drug loading, surface modification, structure adjustment or 3 D technique), various novel membranes and scaffolds that could not only relief inflammation but also influence the biological behaviors of cells have been fabricated to achieve more effective periodontal regeneration. This review provides an overview of the usage of electrospinning materials in treatments of periodontitis, in order to get to know the existing research situation and find treatment breakthroughs of the periodontal diseases.

Recent Advances of Useful Cell Sources in the Periodontal Regeneration

2019 ◽  
Vol 14 (1) ◽  
pp. 3-8 ◽  
Author(s):  
Young-Dan Cho ◽  
Kyoung-Hwa Kim ◽  
Hyun-Mo Ryoo ◽  
Yong-Moo Lee ◽  
Young Ku ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Tissue Regeneration ◽  
Periodontal Regeneration ◽  
Periodontal Tissue ◽  
Therapy Outcomes ◽  
Differentiated Cells ◽  
Periodontal Tissue Regeneration ◽  
Cell Sources ◽  
Induced Pluripotent

Background: Periodontitis is an inflammatory disease that can result in destruction of the tooth attachment apparatus. Therefore, periodontal tissue regeneration is currently an important focus of research in the field. Approaches using stem cells and reprogrammed cells, such as induced pluripotent stem cells (iPSCs) or trans-differentiated cells, represent the cutting edge in periodontal regeneration, and have led to many trials for their clinical application. Objectives and Results: In this review, we consider all available stem cell sources, methods to obtain the cells, their capability to differentiate into the desired cells, and the extent of their utilization in periodontal regeneration. In addition, we introduce the new concepts of using iPSCs and transdifferentiated cells for periodontal regeneration. Finally, we discuss the promise of tissue engineering for improving cell therapy outcomes for periodontal regeneration. Conclusions: Despite their limitations, iPSCs and trans-differentiated cells may be promising cell sources for periodontal tissue regeneration. Further collaborative investigation is required for the effective and safe application of these cells in combination with tissue engineering elements, like scaffolds and biosignals.

Regenereation in periodontics

2018 ◽  
Author(s):  
Murtaza Kaderi ◽  
Mohsin Ali ◽  
Alfiya Ali ◽  
Tasneem Kaderi
Keyword(s):  
Tissue Engineering ◽  
Clinical Practice ◽  
Periodontal Disease ◽  
Disease Progression ◽  
Tissue Regeneration ◽  
Surgical Procedures ◽  
Periodontal Regeneration ◽  
Guided Tissue Regeneration ◽  
Periodontal Tissues ◽  
Extensive Documentation

The goals of periodontal therapy are to arrest of periodontal disease progression and to attain the regeneration of the periodontal apparatus. Osseous grafting and Guided tissue regeneration (GTR) are the two techniques with the most extensive documentation of periodontal regeneration. However, these techniques offer limited potential towards regenerating the periodontal tissues. Recent surgical procedures and application of newer materials aim at greater and more predictable regeneration with the concept of tissue engineering for enhanced periodontal regeneration and functional attachment have been developed, analyzed, and employed in clinical practice

scholarly journals Surgical Management of Horizontal Root Fracture with Platelet Rich Fibrin (PRF) Placement: Case Report of Two Cases

2013 ◽  
Vol 01 (02) ◽  
pp. 125-128
Author(s):  
Parul Bansal ◽  
Kalpana Kanyal ◽  
Vineeta Nikhil
Keyword(s):  
Case Report ◽  
Tissue Regeneration ◽  
Surgical Approach ◽  
Permanent Teeth ◽  
Periodontal Tissue ◽  
Root Fracture ◽  
Platelet Rich Fibrin ◽  
Periodontal Tissue Regeneration ◽  
Root Fractures ◽  
Horizontal Root Fracture

AbstractRadicular fractures in permanent teeth are uncommon injuries among dental traumas, being only 0.5 - 7% of the cases. Horizontal root fractures can be managed endodontically or combined endodontic and surgical approach. Treatment varies according to the displacement and vitality of the fragments. This paper presents a case report of two cases of horizontal root fracture, present between the middle and apical third of central incisors, which were managed by combined endodontic and surgical approach, while in second case it was followed by PRF placement to facilitate osteoinduction and periodontal tissue regeneration.

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