scholarly journals Temporal response of an injectable calcium phosphate material in a critical size defect

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Jacob T. Landeck ◽  
William R. Walsh ◽  
Rema A. Oliver ◽  
Tian Wang ◽  
Mallory R. Gordon ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Minimally Invasive ◽  
Bone Substitute ◽  
Critical Size ◽  
Working Time ◽  
Histological Evaluation ◽  
Radiographic Evaluation ◽  
Bone Substitute Material ◽  
Substitute Material

Abstract Background Calcium phosphate-based bone graft substitutes are used to facilitate healing in bony defects caused by trauma or created during surgery. Here, we present an injectable calcium phosphate-based bone void filler that has been purposefully formulated with hyaluronic acid to offer a longer working time for ease of injection into bony defects that are difficult to access during minimally invasive surgery. Methods The bone substitute material deliverability and physical properties were characterized, and in vivo response was evaluated in a critical size distal femur defect in skeletally mature rabbits to 26 weeks. The interface with the host bone, implant degradation, and resorption were assessed with time. Results The calcium phosphate bone substitute material could be injected as a paste within the working time window of 7–18 min, and then self-cured at body temperature within 10 min. The material reached a maximum ultimate compressive strength of 8.20 ± 0.95 MPa, similar to trabecular bone. The material was found to be biocompatible and osteoconductive in vivo out to 26 weeks, with new bone formation and normal bone architecture observed at 6 weeks, as demonstrated by histological evaluation, microcomputed tomography, and radiographic evaluation. Conclusions These findings show that the material properties and performance are well suited for minimally invasive percutaneous delivery applications.

scholarly journals In Vivo Analysis of the Biocompatibility and Bone Healing Capacity of a Novel Bone Grafting Material Combined with Hyaluronic Acid

2021 ◽  
Vol 22 (9) ◽  
pp. 4818
Author(s):  
Annica Pröhl ◽  
Milijana Batinic ◽  
Said Alkildani ◽  
Michael Hahn ◽  
Milena Radenkovic ◽  
...  
Keyword(s):  
Bone Substitute ◽  
Sham Operation ◽  
Bone Substitute Material ◽  
Water Binding ◽  
Sham Operation Group ◽  
Operation Group ◽  
Substitute Material ◽  
Tissue Reactions ◽  
Inflammatory Tissue

The present in vivo study analyses both the inflammatory tissue reactions and the bone healing capacity of a newly developed bone substitute material (BSM) based on xenogeneic bone substitute granules combined with hyaluronate (HY) as a water-binding molecule. The results of the hyaluronate containing bone substitute material (BSM) were compared to a control xenogeneic BSM of the same chemical composition and a sham operation group up to 16 weeks post implantationem. A major focus of the study was to analyze the residual hyaluronate and its effects on the material-dependent healing behavior and the inflammatory tissue responses. The study included 63 male Wistar rats using the calvaria implantation model for 2, 8, and 16 weeks post implantationem. Established and Good Laboratory Practice (GLP)-conforming histological, histopathological, and histomorphometrical analysis methods were conducted. The results showed that the new hyaluronate containing BSM was gradually integrated within newly formed bone up to the end of the study that ended in a condition of complete bone defect healing. Thereby, no differences to the healing capacity of the control BSM were found. However, the bone formation in both groups was continuously significantly higher compared to the sham operation group. Additionally, no differences in the (inflammatory) tissue response that was analyzed via qualitative and (semi-) quantitative methods were found. Interestingly, no differences were found between the numbers of pro- and anti-inflammatory macrophages between the three study groups over the entire course of the study. No signs of the HY as a water-binding part of the BSM were histologically detectable at any of the study time points, altogether the results of the present study show that HY allows for an optimal material-associated bone tissue healing comparable to the control xenogeneic BSM. The added HY seems to be degraded within a very short time period of less than 2 weeks so that the remaining BSM granules allow for a gradual osteoconductive bone regeneration. Additionally, no differences between the inflammatory tissue reactions in both material groups and the sham operation group were found. Thus, the new hyaluronate containing xenogeneic BSM and also the control BSM have been shown to be fully biocompatible without any differences regarding bone regeneration.

Bone Regenerative Properties of Injectable Calcium Phosphate/PLGA Cement in an Alveolar Bone Defect

2012 ◽  
Vol 529-530 ◽  
pp. 300-303 ◽  
Author(s):  
R.P. Félix Lanao ◽  
J.W.M. Hoekstra ◽  
Joop G.C. Wolke ◽  
Sander C.G. Leeuwenburgh ◽  
A.S. Plachokova ◽  
...  
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Calcium Phosphate ◽  
Bone Formation ◽  
Bone Substitute ◽  
Alveolar Bone ◽  
Bone Defects ◽  
Plga Microspheres ◽  
Bone Substitute Material ◽  
Substitute Material

Periodontitis is one of the most common inflammatory diseases, which can lead to early tooth loss. The conventional treatment of periodontitis is to arrest the disease progression. Most reconstructive procedures involve application of bone substitutes, barrier membranes or a combination of both into the bony defects. Calcium phosphate cements (CPCs) are the predominant type of bone substitute material used for reasons of injectability and hence perfect filling potential for bone defects. Recently, injectable apatitic CPCs demonstrated to be more rapidly degradable when combined with poly (lactic-co-glycolic) acid (PLGA) microspheres. Further, PLGA microspheres can be used as a delivery vehicle for growth factors. In this study, the performance of injectable CPCs as a bone substitute material for alveolar bone defects created in Beagle dogs was evaluated. Four CPC-formulations were generated by incorporating hollow or dense PLGA microspheres, either or not loaded with the growth factors (platelet derived growth factor (PDGF) and insulin-like growth factor (IGF). Implantation period was 8 weeks. Bone formation was based on histological and histomorphometrical evaluation. The results demonstrated that filling alveolar bone defects with CPC-dense PLGA revealed significant more bone formation compared to CPC-hollow PLGA either or not loaded with IGF and PDGF. In summary, we conclude that injectable CPC-dense PLGA composites proved to be the most suitable material for a potential use as off the shelf material due to its good biocompatibility, enhanced degradability and subsequent bone formation.

scholarly journals Cell response of calcium phosphate based ceramics, a bone substitute material

2013 ◽  
Vol 16 (4) ◽  
pp. 703-712 ◽  
Author(s):  
Juliana Marchi ◽  
Christiane Ribeiro ◽  
Ana Helena de Almeida Bressiani ◽  
Márcia Martins Marques
Keyword(s):  
Calcium Phosphate ◽  
Cell Response ◽  
Bone Substitute ◽  
Bone Substitute Material ◽  
Substitute Material

Carvable calcium phosphate bone substitute material

2007 ◽  
Vol 83B (1) ◽  
pp. 1-8 ◽  
Author(s):  
M. P. Hofmann ◽  
U. Gbureck ◽  
C. O. Duncan ◽  
M. S. Dover ◽  
J. E. Barralet
Keyword(s):  
Calcium Phosphate ◽  
Bone Substitute ◽  
Bone Substitute Material ◽  
Substitute Material

Rheological Properties and Injectability of a Calcium Phosphate Bone Substitute Material

2005 ◽  
Vol 288-289 ◽  
pp. 557-560 ◽  
Author(s):  
H. Wang ◽  
Xiao Ping Wang ◽  
Jian Dong Ye ◽  
Ying Jun Wang ◽  
Ping Gen Rao
Keyword(s):  
Calcium Phosphate ◽  
Rheological Properties ◽  
Rheological Behavior ◽  
Bone Substitute ◽  
Clinical Applications ◽  
Bone Substitute Material ◽  
Substitute Material ◽  
Effects Of Temperature

A calcium phosphate bone substitute material was prepared and its rheological behavior and injectability were studied in this work. The effects of temperature, L/P ratio and adjuvant on the rheological properties and injectability of the pastes were discussed. The results show that the calcium phosphate bone substitute material is injectable with good fluidity and is suitable for the clinical applications. The rheological behavior and injectability of the bone substitute material can be improved by adding adjuvants and optimizing L/P ratio.

Effects of autologous platelet rich plasma gel and calcium phosphate biomaterials on bone healing in an ulnar ostectomy model in dogs

2009 ◽  
Vol 22 (06) ◽  
pp. 460-466 ◽  
Author(s):  
J.-G. Grand ◽  
E. Dalibert ◽  
B. Fellah ◽  
O. Gauthier ◽  
G.W. Niebauer ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Formation ◽  
Bone Healing ◽  
Critical Size ◽  
Platelet Rich Plasma ◽  
Long Bone ◽  
Histological Evaluation ◽  
Radiographic Evaluation ◽  
New Bone Formation ◽  
Prp Gel

SummaryThe aim of the study was to evaluate the bone healing properties of an osteopromotive platelet rich plasma (PRP) gel in combination with osteoconductive calcium phosphate (CaP) ceramic granules in a long-bone critical size defect in dogs. A standardised 2 cm long ulnar ostectomy was performed bilaterally in four dogs to compare new-bone formation by CaP matrix with and without association with PRP. Radiographic and histological evaluations were performed blindly. Radiographic evaluation was performed at three, six, nine, 12 and 16 weeks postoperatively. Quantitative measurements of new-bone formation were compared using statistical analysis. At explantation 16 weeks after surgery, no significant ossification was present, neither with CaP granules alone nor in association with PRP gel, and there was no difference of radiodensity between the groups. Qualitative histological evaluation demonstrated for both types of implants the presence of non-mineralised fibrous connective tissue around the CaP granules. New-bone formation was only present to a very small extent within the macropores of the CaP granules at the distal boneimplant interface. In our model which exhibited very limited osteoconduction, neither the CaP granules alone nor in association with PRP were sufficient to stimulate bone healing. In this canine model employing a critical size ulnar gap, the combination of CaP granules and PRP did not effectively promote bone regeneration.

Sign in / Sign up

Export Citation Format

Share Document