scholarly journals Microscopic Image Segmentation and Morphological Characterization of Novel Chitosan/Silica Nanoparticle/Nisin Films Using Antimicrobial Technique for Blueberry Preservation

Membranes ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 303
Author(s):  
Rokayya Sami ◽  
Schahrazad Soltane ◽  
Mahmoud Helal
Keyword(s):  
Ph Value ◽  
Silica Nanoparticle ◽  
Morphological Characterization ◽  
Aerobic Bacteria ◽  
Nano Materials ◽  
Elongation At Break ◽  
Stable Suspension ◽  
Average Size ◽  
Material Ductility

In the current work, the characterization of novel chitosan/silica nanoparticle/nisin films with the addition of nisin as an antimicrobial technique for blueberry preservation during storage is investigated. Chitosan/Silica Nanoparticle/N (CH-SN-N) films presented a stable suspension as the surface loads (45.9 mV) and the distribution was considered broad (0.62). The result shows that the pH value was increased gradually with the addition of nisin to 4.12, while the turbidity was the highest at 0.39. The content of the insoluble matter and contact angle were the highest for the Chitosan/Silica Nanoparticle (CH-SN) film at 5.68%. The use of nano-materials in chitosan films decreased the material ductility, reduced the tensile strength and elongation-at-break of the membrane. The coated blueberries with Chitosan/Silica Nanoparticle/N films reported the lowest microbial contamination counts at 2.82 log CFU/g followed by Chitosan/Silica Nanoparticle at 3.73 and 3.58 log CFU/g for the aerobic bacteria, molds, and yeasts population, respectively. It was observed that (CH) film extracted 94 regions with an average size of 449.10, at the same time (CH-SN) film extracted 169 regions with an average size of 130.53. The (CH-SN-N) film presented the best result at 5.19%. It could be observed that the size of the total region of the fruit for the (CH) case was the smallest (1663 pixels), which implied that the fruit lost moisture content. As a conclusion, (CH-SN-N) film is recommended for blueberry preservation to prolong the shelf-life during storage.

scholarly journals Identification and Characterization of Citrus Chlorotic Spot Virus, a New Dichorhavirus Associated with Citrus Leprosis-Like Symptoms

Plant Disease ◽  
2018 ◽  
Vol 102 (8) ◽  
pp. 1588-1598 ◽  
Author(s):  
C. Chabi-Jesus ◽  
P. L. Ramos-González ◽  
A. D. Tassi ◽  
O. Guerra-Peraza ◽  
E. W. Kitajima ◽  
...  
Keyword(s):  
Virus Strain ◽  
Viral Vectors ◽  
Morphological Characterization ◽  
Spot Virus ◽  
Chlorotic Spot ◽  
Bipartite Genome ◽  
Citrus Leprosis ◽  
Average Size ◽  
Parenchymal Cells

Local chlorotic spots resembling early lesions characteristic of citrus leprosis (CL) were observed in leaves of two sweet orange (Citrus sinensis L.) trees in Teresina, State of Piauí, Brazil, in early 2017. However, despite the similarities, these spots were generally larger than those of a typical CL and showed rare or no necrosis symptoms. In symptomatic tissues, transmission electron microscopy revealed the presence of viroplasms in the nuclei of the infected parenchymal cells and rod-shaped particles with an average size of approximately 40 × 100 nm, resembling those typically observed during infection by dichorhaviruses. A bipartite genome of the putative novel virus, tentatively named citrus chlorotic spot virus (CiCSV) (RNA1 = 6,518 nucleotides [nt] and RNA2 = 5,987 nt), revealed the highest nucleotide sequence identity values with the dichorhaviruses coffee ringspot virus strain Lavras (73.8%), citrus leprosis virus N strain Ibi1 (58.6%), and orchid fleck virus strain So (56.9%). In addition to citrus, CiCSV was also found in local chlorotic lesions on leaves of the ornamental plant beach hibiscus (Talipariti tiliaceum (L.) Fryxell). Morphological characterization of mites recovered from the infected plants revealed at least two different types of Brevipalpus. One of them corresponds to Brevipalpus yothersi. The other is slightly different from B. yothersi mites but comprises traits that possibly place it as another species. A mix of the two mite types collected on beach hibiscus successfully transmitted CiCSV to arabidopsis plants but additional work is required to verify whether both types of flat mite may act as viral vectors. The current study reveals a newly described dichorhavirus associated with a citrus disease in the northeastern region of Brazil.

Morphological Characterization of Chitosan in the Hydroxyapatite/Chitosan Nanocomposites

2009 ◽  
Vol 79-82 ◽  
pp. 1675-1678
Author(s):  
Xiao Ying Lu ◽  
Xiu Hong Wang ◽  
Jian Xin Wang ◽  
Shu Xin Qu ◽  
Jie Weng
Keyword(s):  
Ph Value ◽  
Biomedical Application ◽  
Morphological Characterization ◽  
Layer By Layer ◽  
Oriented Growth ◽  
Hydrothermal Temperature ◽  
Morphological Differences ◽  
Hydrothermal Precipitation

The morphological differences of chitosan (CS) in the hydroxyapatite (HA)/CS nanocomposites were investigated in detailed, which were prepared via in situ hydrothermal precipitation. The results show that the obtained nanocomposites have excellent crystallinity and the crystal has excellent ordered structure, which is important to the composites performances in the biomedical application. Moreover, the CS arrangement and crystallinity in the composites greatly depend on the hydrothermal temperature and the pH value of precipitating agent. The temperature ranging from 373 to 413K and pH value of precipitating agent ranging from 12 to 14 were favorable to the crystallization and oriented growth of CS molecules in the composites. The CS crystals with better arrangement are assembled in the order of layer-by-layer in these composites.

scholarly journals Mineralogical, Physicochemical and Morphological Characterization of Okpella Clay

Mining Revue ◽  
2021 ◽  
Vol 27 (4) ◽  
pp. 78-82
Author(s):  
Nurudeen Salahudeen ◽  
Aminat Oluwafisayo Abodunrin
Keyword(s):  
Ph Value ◽  
Chemical Characterization ◽  
Morphological Characterization ◽  
X Ray Diffraction ◽  
Local Clay ◽  
Mineralogical Characterization ◽  
X Ray ◽  
Edo State ◽  
Fluorescence Analyzer

Abstract Local clay mineral was mined from Okpella Town, Etsako Local Government Area of Edo State, Nigeria. Mineralogical characterization of the clay was carried out using X-ray diffraction analyzer. Chemical characterization of the clay was carried out using X-ray fluorescence analyzer and the pH analysis of the clay was carried out using pH meter. The mineralogical analysis revealed that the clay was majorly a dolomite mineral having 72% dolomite. The impurities present are 18% cristobalite, 4.1% garnet, 5% calcite and 1% quicklime. The pH analysis of the clay revealed that the clay was acidic having average pH value of 3.9. The pH determined for the 1:1, 1:2, 1:4, 1:8 and 1:10 samples were 3.61, 3.85, 3.85, 4.05 and 4.09, respectively.

A novel approach for 3D morphological characterization of silica nanoparticle population through HAADF-STEM

Measurement ◽  
2021 ◽  
pp. 109521
Author(s):  
Loïc Crouzier ◽  
Frédéric Pailloux ◽  
Alexandra Delvallée ◽  
Laurent Devoille ◽  
Nicolas Feltin ◽  
...  
Keyword(s):  
Silica Nanoparticle ◽  
Morphological Characterization ◽  
Novel Approach

Isolation and Characterization of Halotolerant Aerobic Bacteria From Oil Reservoir

2012 ◽  
Author(s):  
Rosli Md. Illias ◽  
Seok Wei Ooi ◽  
Ahmad Kamal Idris ◽  
Wan Aizan Wan Abdul Rahman
Keyword(s):  
Oil Recovery ◽  
Sole Carbon Source ◽  
Morphological Characterization ◽  
Aerobic Bacteria ◽  
Oil Reservoir ◽  
Genus Bacillus ◽  
Isolation And Characterization ◽  
Tension Tests ◽  
Naci Concentration

Beberapa bakteria halotoleran telah berjaya dipencilkan daripada sampel air masin telaga minyak Semangkok di mana ujian biokimia dan morfologi telah dijalankan bagi bakteria ini. Hampir semua bakteria yang dipencilkan tergolong di dalam genus Bacillus. Sebahagian besar daripada pencilan berkeupayaan untuk hidup di dalam medium pertumbuhan yang mengandungi kerosen sebagai sumber karbon utama dan tenaga dan berkeupayaan untuk hidup di dalam medium dengan kepekatan NaCI diantara 10–15%. Ujian ketegangan antara permukaan (IFT) menunjukkan pencilan menghasilkan biosurfaktan. Enam daripada sembilan pencilan menghasilkan eksopolisakarida di dalam medium pertumbuhan yang mungkin penting di dalam MEOR. Kata kunci: Bakteria; pencirian; pengasingan; Surfaktan; Eksopolisakarida Several halotolerant bacteria were isolated from brine samples from Semangkok oil reservoir. Biochemical and morphological characterization of the bacteria were carried out. These bacteria are gram positive spore formers and have been identified as belonging to the genus Bacillus. Most of the isolates could grow in medium containing kerosene as sole carbon source and energy and tolerate NaCI concentration up to 15%. Interfacial tension and surface tension tests showed that the bacteria were capable of producing biosurfactant. Six out of nine were able to produce exopolysaccharide. We believe these isolate could be appointed as future biopolymer producer especially for microbial enhanced oil recovery (MEOR). Keywords: Bacteria; Characterization; Isolation; Surfactant; Exopolysaccharides.

scholarly journals Controlled size and high stability SiO2@ZnO QD’s with high green emission obtained by a simple colloidal route

2019 ◽  
Author(s):  
Rene Perez Cuapio ◽  
Jose Alberto A G Alvarado Garcia ◽  
Mauricio Pacio Castillo ◽  
Antonio Arce Plaza ◽  
Carlos Bueno Avendaño ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Green Emission ◽  
Morphological Characterization ◽  
The Core ◽  
Ft Ir ◽  
Uv Lamp ◽  
Size And Morphology ◽  
The Stability ◽  
Average Size

In this work the synthesis of visible ZnO quantum dots (QDs) functionalized/stabilized with oleic acid and core-shelled with SiO2 is presented. A colloidal route was followed to synthesize ZnO QDs with 5 nm average size. Several characterizations were done to identify and assure the core, the stabilization, grain size and QDs emission characteristics. Techniques like FT-IR, XRD, TEM, SEM, UV-Vis, PL and an UV lamp were used to test composition, structural, morphological and emission of the as obtained quantum dots. After the structural and morphological characterization of the particles, they were functionalized with (3-aminopropyl) trimethoxysilane (APTMS).  It was observed that the size and morphology are unchanged but the stability depends directly on the APTMS oversaturation which is due to the formation of a thick SiO2 layer. From the PL characterization results it was noticed that the strong green emission is related to the APTMS content and directly related to the shell formation, this emission decreases as the core-shell thickness increases.

scholarly journals STEREOLOGY OF NANO-MATERIALS

2011 ◽  
Vol 29 (1) ◽  
pp. 1 ◽  
Author(s):  
Tomasz Wejrzanowski ◽  
Małgorzata Lewandowska ◽  
Krzysztof J Kurzydłowski
Keyword(s):  
Early Stage ◽  
Quantitative Description ◽  
Nano Materials ◽  
Structured Materials ◽  
Modern Engineering ◽  
Size Diversity ◽  
High Resolution Images ◽  
Average Size ◽  
Nano Grains

Nano-structured materials attract a growing attention due to their superior mechanical and physical properties. Such properties are inherently related to the unique structure which is controlled at the nano-scale. In the early stage of their development, the structural characterization of nano-materials was limited to the average grain/particle size. However, nano-grains/particles form stochastic populations with diverse size and shape. The recent experimental and theoretical results show that in addition to the average size, size diversity of grains/particles has a significant effect on the properties of nano-materials. This rationalizes more advanced description of the structures of nano-materials. Advanced microscopic methods are now available to image microstructure of nano-materials, e.g., HRSTEM and AFM. The ultra-high resolution images can be efficiently processed to obtain quantitative description of the nano-grains/particles. Examples are shown how such a description can be used for optimizing the microstructures of modern engineering nano-materials.

scholarly journals Degradation of Meropenem by Heterogeneous Photocatalysis Using TiO2/Fiberglass Substrates

Catalysts ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 344
Author(s):  
Alejandro Altamirano Briones ◽  
Iván Cóndor Guevara ◽  
David Mena ◽  
Isabel Espinoza ◽  
Christian Sandoval-Pauker ◽  
...  
Keyword(s):  
Ph Value ◽  
Oxygen Demand ◽  
Morphological Characterization ◽  
Heterogeneous Photocatalysis ◽  
Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Ph Values ◽  
Fourth Cycle ◽  
Regeneration Procedure

Meropenem (MER), a carbapenem, is considered a last-resort antibiotic. Its presence in water bodies, together with other antibiotics, has brought about environmental problems related to the destruction of natural microorganisms and the development of antibiotic-resistant bacteria. Herein, the degradation of MER by heterogeneous photocatalysis using TiO2 immobilized on fiberglass substrates is reported. Morphological characterization of the substrates was performed by Scanning Electron Microscopy (SEM). Three pH values (4.0, 5.7, and 7.9) were tested for the treatment of MER solutions (100 mg/L). The best rate constants and MER removals were obtained at pH 4.0 (0.032 min−1; 83.79%) and 5.7 (0.032 min−1; 83.48%). Chemical Oxygen Demand (COD) and Total Organic Carbon (TOC) removals of 25.80% and 29.60% were achieved for the treatment at a pH value of 5.7. The reuse and regeneration of the plates were also tested. The activity of the substrates was maintained until the fourth cycle of reuse, nonetheless, a decrease in MER removal was observed for the 5th cycle. After the fourth cycle of reuse, the activity of the substrates was recovered by a regeneration procedure involving a wash stage of the substrates with a 1% H2O2 solution in an ultrasonic bath.

Morphological Characterization of Mycobacteriophage R1

1974 ◽  
Vol 32 ◽  
pp. 254-255
Author(s):  
B. L. Soloff ◽  
T. A. Rado
Keyword(s):  
Carbon Source ◽  
Stationary Phase ◽  
Growth Phase ◽  
Minimal Medium ◽  
Morphological Characterization ◽  
Logarithmic Growth Phase ◽  
Complete Lysis ◽  
Lysogenic Culture ◽  
Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

Morphological Characterization of Rhinacanthus Species of Sri Lanka

Planta Medica ◽  
2010 ◽  
Vol 76 (05) ◽  
Author(s):  
APPR Amarasinghe ◽  
RP Karunagoda ◽  
DSA Wijesundara
Keyword(s):  
Sri Lanka ◽  
Morphological Characterization
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