scholarly journals Prevalence and susceptibility to antibiotics from Campylobacter jejuni and Campylobacter coli isolated from chicken meat in southern Benin, West Africa

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Sylvain Daton Kouglenou ◽  
Alidehou Jerrold Agbankpe ◽  
Victorien Dougnon ◽  
Armando Djiyou Djeuda ◽  
Esther Deguenon ◽  
...  
Keyword(s):  
West Africa ◽  
Campylobacter Jejuni ◽  
Chicken Meat ◽  
Campylobacter Coli ◽  
Southern Benin

scholarly journals Comparison of Genotypes and Antibiotic Resistances of Campylobacter jejuni and Campylobacter coli on Chicken Retail Meat and at Slaughter

2013 ◽  
Vol 79 (12) ◽  
pp. 3875-3878 ◽  
Author(s):  
Sonja Kittl ◽  
Bożena M. Korczak ◽  
Lilian Niederer ◽  
Andreas Baumgartner ◽  
Sabina Buettner ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Human Infection ◽  
Chicken Meat ◽  
Campylobacter Coli ◽  
Production Chain ◽  
Content Type ◽  
Resistance Patterns ◽  
Retail Meat ◽  
Retail Chicken Meat ◽  
Antibiotic Resistance Patterns

ABSTRACTMultilocus sequence typing (MLST) and antibiotic resistance patterns ofCampylobacter jejuniandCampylobacter colifrom retail chicken meat showed high overlap with isolates collected at slaughterhouses, indicating little selection along the production chain. They also showed significant common sequence types with human clinical isolates, revealing chicken meat as a likely source for human infection.

Prevalence and Antimicrobial Resistance of Campylobacter jejuni and Campylobacter coli Isolated from Raw Chicken Meat and Human Stools in Korea

2006 ◽  
Vol 69 (12) ◽  
pp. 2915-2923 ◽  
Author(s):  
YUN-SOOK KANG ◽  
YONG-SUN CHO ◽  
SUN-KYUNG YOON ◽  
MYEONG-AE YU ◽  
CHANG-MIN KIM ◽  
...  
Keyword(s):  
Nalidixic Acid ◽  
Campylobacter Jejuni ◽  
Seasonal Changes ◽  
Chicken Meat ◽  
Seasonal Effect ◽  
Campylobacter Coli ◽  
Retail Stores ◽  
Department Stores ◽  
Isolation Rate ◽  
Traditional Markets

Prevalence of Campylobacter in raw chicken meat and human stools and subsequent antibiotic resistance profiles of the pathogenic isolates obtained from 2000 through 2002 were investigated. Campylobacter jejuni and Campylobacter coli were isolated from 570 of the 923 raw chicken meat samples collected from traditional markets, large retail stores, or department stores in Korea, resulting in the isolation rate of 61.8%. A total of 579 Campylobacter isolates were obtained from raw chicken (36.3% for C. jejuni and 26.4% for C. coli) with the average population of 335.6 CFU/g. From 513 human stool samples, 15 isolates of Campylobacter were detected. Seasonal variation in the quantification of C. coli was not noticeable throughout the year, while the isolation rate of C. jejuni was the highest in September through October (840 CFU/g) followed by that of July through August and May through June in decreasing order, showing a significant seasonal effect (P < 0.05). Contamination of Campylobacter was more severe in raw chicken meat sold in traditional markets than in those sold in large retail stores and department stores. Prevalence of Campylobacter in raw chicken sold in traditional markets was significantly influenced by seasonal changes (P < 0.05), whereas the samples obtained from other places was less affected by the seasonal changes. Susceptibilities of the 594 chicken isolates to ciprofloxaxin, chloramphenicol, erythromycin, kanamycin, nalidixic acid, and tetracycline were determined by an E-test. Campylobacter isolates were the most resistant to nalidixic acid (91.4%) followed by ciprofloxaxin (87.9%), tetracycline (87.2%), kanamycin (30.6%), erythromycin (19.4%), and chloramphenicol (1.3%). Human isolates showed a similar resistance to the six antibiotics tested. The proportion of Campylobacter isolates with multidrug resistance to four or more antimicrobials obtained from 2000 through 2002 ranged from 28 to 43.5%, indicating that it could be a serious health-threatening factor. This study suggests that it is prudent to establish an effective National Monitoring Program in Korea for the prevention and control of Campylobacter spp.

scholarly journals Comparison of Antimicrobial Resistance of Campylobacter jejuni and Campylobacter coli Isolated from Humans and Chicken Carcasses in Poland

2008 ◽  
Vol 71 (3) ◽  
pp. 602-607 ◽  
Author(s):  
ELŻBIETA ROŻYNEK ◽  
KATARZYNA DZIERŻANOWSKA-FANGRAT ◽  
DOROTA KORSAK ◽  
PIOTR KONIECZNY ◽  
SEBASTIAN WARDAK ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Campylobacter Jejuni ◽  
Genetic Background ◽  
Broiler Chicken ◽  
Chicken Meat ◽  
Campylobacter Coli ◽  
Drug Resistant ◽  
Stool Samples ◽  
Campylobacter Infection ◽  
Foodborne Infection

Campylobacter-associated gastroenteritis remains an important cause of morbidity worldwide, and some evidence suggests that poultry is an important source of this foodborne infection in humans. This study was conducted to analyze the prevalence and genetic background of resistance of 149 Campylobacter jejuni and 54 Campylobacter coli strains isolated from broiler chicken carcasses and from stool samples of infected children in Poland from 2003 through 2005. Nearly all isolates were susceptible to macrolides and aminoglycosides. The highest resistance in both human and chicken strains was observed for ciprofloxacin (more than 40%), followed by ampicillin (13 to 21%), and tetracycline (8 to 29%). Resistance to ampicillin and tetracycline rose significantly between 2003 and 2005. Slight differences in resistance between human and chicken isolates indicate that although chicken meat is not the only source of Campylobacter infection in our population, it can be involved in the transmission of drug-resistant Campylobacter strains to humans.

scholarly journals Comparison of Loop-Mediated Isothermal Amplification Assay and Conventional Culture Methods for Detection of Campylobacter jejuni and Campylobacter coli in Naturally Contaminated Chicken Meat Samples

2009 ◽  
Vol 75 (6) ◽  
pp. 1597-1603 ◽  
Author(s):  
Wataru Yamazaki ◽  
Masumi Taguchi ◽  
Takao Kawai ◽  
Kentaro Kawatsu ◽  
Junko Sakata ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Lamp Assay ◽  
Chicken Meat ◽  
Isothermal Amplification ◽  
Campylobacter Coli ◽  
Culture Methods ◽  
Culture Test ◽  
Loop Mediated Isothermal Amplification ◽  
Conventional Culture ◽  
Meat Samples

ABSTRACT We investigated the efficacy of a loop-mediated isothermal amplification (LAMP) assay for detection of chicken meat samples naturally contaminated with Campylobacter jejuni and Campylobacter coli. A total of 144 Preston enrichment broth cultures from chicken meat samples were assessed by using the LAMP assay and conventional culture methods, which consist of a combination of Preston enrichment culturing and plating onto Butzler and modified charcoal cefoperazone deoxycholate agars. Compared with C. jejuni-C. coli isolation using the conventional culture test, the LAMP results showed 98.5% (67/68) and 97.4% (74/76) sensitivity and specificity, respectively, and the positive and negative predictive values were 97.1% (67/69) and 98.7% (74/75), respectively. The conventional culture test required more than 3 to 4 days to isolate and identify C. jejuni and C. coli in the Preston enrichment cultures. In contrast, the LAMP assay was markedly faster, requiring less than 90 min from the beginning of DNA extraction to final detection and differentiation of C. jejuni and C. coli. In total, the LAMP assay required 23.5 to 25.5 h from the beginning of the enrichment culture to final determination. These results suggest that our LAMP assay is a powerful tool for rapid, sensitive, and practical detection of C. jejuni and C. coli which may facilitate surveillance and control of C. jejuni-C. coli contamination in chicken, as well as investigations of food poisoning incidents caused by these organisms. This is the first report of a highly sensitive and specific LAMP assay to detect and differentiate C. jejuni and C. coli in chicken meat samples.

scholarly journals Microbiological Safety of Leafy Vegetables Produced at Houeyiho and Sèmè-Kpodji Vegetable Farms in Southern Benin: Risk Factors for Campylobacter spp.

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Sylvain Daton Kougblénou ◽  
Alidéhou Jerrold Agbankpé ◽  
Justin Gbèssohélé Béhanzin ◽  
Tamègnon Victorien Dougnon ◽  
Alidah Victonie Aniambossou ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Irrigation Water ◽  
Poultry Manure ◽  
Food Poisoning ◽  
Leafy Vegetables ◽  
Contamination Rate ◽  
Campylobacter Coli ◽  
Poultry Products ◽  
Campylobacter Spp ◽  
Southern Benin

Foodborne infections, mainly those attributable to Campylobacter, are one of the most common causes of intestinal diseases, of bacterial origin in humans. Although the vehicle of transmission is not always identified, the most common vehicles are poultry, poultry products, and contaminated water. In Southern Benin, an excessive use of poultry manure as fertilizer in vegetable farms was noted. This survey aimed to determine the prevalence and concentration of Campylobacter spp., especially Campylobacter jejuni and Campylobacter coli, in selected environmental samples (poultry manure, and irrigation water) and freshly harvested leafy vegetables in two (Houeyiho and Sèmè-Kpodji) vegetable farms in southern Benin. To achieve this objective, we analyzed 280 samples, including 224 samples of leafy vegetables (Solanum macrocarpon and Lactuca sativa capita), 28 samples of irrigation water, and 28 samples of poultry manure. The analysis of the samples taken was carried out according to the modified NF EN ISO 10272-1 standard. Of the 280 samples analyzed in this survey, 63 were positive for Campylobacter contamination. For leafy vegetable samples analyzed in this survey, the contamination rate was of 15.63%. 60.71% of poultry manure samples analyzed were contaminated with Campylobacter spp. and 39.29% of irrigation water samples were contaminated. The statistical analysis of these results showed that there is a correlation between the contamination of leafy vegetables, poultry manure, and irrigations (p<0.01). Campylobacter jejuni (53.97%) was more involved in contaminations than Campylobacter coli (36.57%). This study has shown that there is a real risk of food poisoning by Campylobacter jejuni and Campylobacter coli among consumers of leafy vegetables in southern Benin. The origin of contamination of these leafy vegetables is poultry manure used as fertilizer in vegetable gardens and irrigation water used.

Rapid Detection of Campylobacter jejuni, Campylobacter coli, and Campylobacter lari in Fresh Chicken Meat and By-Products in Bangkok, Thailand, Using Modified Multiplex PCR

2015 ◽  
Vol 78 (7) ◽  
pp. 1363-1369 ◽  
Author(s):  
S. SAIYUDTHONG ◽  
K. PHUSRI ◽  
S. BUATES
Keyword(s):  
Multiplex Pcr ◽  
Campylobacter Jejuni ◽  
Predictive Value ◽  
Chicken Meat ◽  
Campylobacter Coli ◽  
Fresh Market ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Campylobacter Lari ◽  
By Products

A multiplex PCR assay for simultaneous detection and differentiation of Campylobacter jejuni, Campylobacter coli, and Campylobacter lari was developed and validated to assess the occurrence of these bacteria in fresh chicken meat and by-products in Bangkok, Thailand, by using a new combination of four previously published PCR primers for C. jejuni, C. coli, C. lari, and a universal 16S rDNA gene as an internal control. The specificity was determined by using 13 strains of other bacteria. With pure culture DNA, the detection limit was 0.017 ng/PCR for C. jejuni and C. coli and was 0.016 ng/PCR for C. lari. It can detect 10 CFU of C. jejuni, C. coli, and C. lari in 2 g of chicken meat within a 16-h enrichment time. Our multiplex PCR assay was applied for identification of Campylobacter spp. in 122 supermarket samples and 108 fresh market samples. Of the 230 samples evaluated by multiplex PCR, 54.0, 3.3, and 10.7% of supermarket samples were positive for C. jejuni, C. coli, and mixed C. jejuni and C. coli, respectively, and 56.5 and 33.3% of fresh market samples were positive for C. jejuni and mixed C. jejuni and C. coli, respectively. No sample was positive for C. lari. Fresh market samples had significantly higher C. jejuni and C. coli contamination than those from supermarkets (relative risk: 1.3; P = 0.0001). Compared with the culture method (a gold standard), the sensitivity, specificity, positive predictive value, negative predictive value, and diagnostic accuracy of multiplex PCR were 97.7, 86.8, 96.1, 92.0, and 95.2%, respectively. No significant difference was observed between results from two methods (P = 0.55). Therefore, the established multiplex PCR was not only rapid and easy to perform but had a high sensitivity and specificity to distinguish between C. jejuni, C. coli, and C. lari, even in samples containing mixed contamination. Our study indicated that fresh chicken meat and by-products from fresh markets were significantly less hygienic than those from supermarkets.

scholarly journals Antimicrobial-Resistant Campylobacter Species from Retail Raw Meats

2003 ◽  
Vol 69 (5) ◽  
pp. 3005-3007 ◽  
Author(s):  
Beilei Ge ◽  
David G. White ◽  
Patrick F. McDermott ◽  
Webb Girard ◽  
Shaohua Zhao ◽  
...  
Keyword(s):  
Nalidixic Acid ◽  
Campylobacter Jejuni ◽  
Chicken Meat ◽  
Campylobacter Coli ◽  
Turkey Meat ◽  
Antimicrobial Susceptibilities ◽  
Campylobacter Species ◽  
Raw Meats

ABSTRACT The antimicrobial susceptibilities of 378 Campylobacter isolates were determined. Resistance to tetracycline was the most common (82%), followed by resistance to doxycycline (77%), erythromycin (54%), nalidixic acid (41%), and ciprofloxacin (35%). Campylobacter coli displayed significantly higher rates of resistance to ciprofloxacin and erythromycin than Campylobacter jejuni, and Campylobacter isolates from turkey meat showed a greater resistance than those from chicken meat.

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