Comparison of Genotypes and Antibiotic Resistances of Campylobacter jejuni and Campylobacter coli on Chicken Retail Meat and at Slaughter

ABSTRACTMultilocus sequence typing (MLST) and antibiotic resistance patterns ofCampylobacter jejuniandCampylobacter colifrom retail chicken meat showed high overlap with isolates collected at slaughterhouses, indicating little selection along the production chain. They also showed significant common sequence types with human clinical isolates, revealing chicken meat as a likely source for human infection.

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Antimicrobial Susceptibility Profiles and Molecular Typing of Campylobacter jejuni and Campylobacter coli Isolates from Ducks in South Korea

Applied and Environmental Microbiology ◽

10.1128/aem.02469-14 ◽

2014 ◽

Vol 80 (24) ◽

pp. 7604-7610 ◽

Cited By ~ 24

Author(s):

Bai Wei ◽

Se-Yeoun Cha ◽

Min Kang ◽

Jae-Hee Roh ◽

Hye-Suk Seo ◽

...

Keyword(s):

Campylobacter Jejuni ◽

Multidrug Resistant ◽

Human Infection ◽

Dilution Method ◽

Agar Dilution Method ◽

Campylobacter Coli ◽

Content Type ◽

Food Borne ◽

Production Animals ◽

Susceptibility Profiles

ABSTRACTCampylobacteris a food-borne zoonotic pathogen that causes human gastroenteritis worldwide.Campylobacterbacteria are commensal in the intestines of many food production animals, including ducks and chickens. The objective of the study was to determine the prevalence ofCampylobacterspecies in domestic ducks, and the agar dilution method was used to determine resistance of the isolates to eight antibiotics. In addition, multilocus sequence typing (MLST) was performed to determine the sequence types (STs) of selectedCampylobacterisolates. Between May and September 2012, 58 duck farms were analyzed, and 56 (96.6%) were positive forCampylobacter. Among the isolates, 82.1% wereCampylobacter jejuni, 16.1% wereC. coli, and one was unidentified by PCR. Of the 46C. jejuniisolates, 87.0%, 10.9%, and 21.7% were resistant to ciprofloxacin, erythromycin, and azithromycin, respectively. Among theC. coliisolates, all 9 strains were resistant to ampicillin, and 77.8% and 33.3% were resistant to ciprofloxacin and azithromycin, respectively. The majority of theCampylobacterisolates were classified as multidrug resistant. Twenty-eight STs were identified, including 20 STs forC. jejuniand 8 STs forC. coli. The most common clonal complexes inC. jejuniwere the ST-21 complex and the ST-45 complex, while the ST-828 complex predominated inC. coli. The majority of isolates were of STs noted in ducks and humans from earlier studies, along with seven STs previously associated only with human disease. These STs overlapped between duck and human isolates, indicating thatCampylobacterisolates from ducks should be considered potential sources of human infection.

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Prevalence, Antibiograms, and Transferable tet(O) Plasmid of Campylobacter jejuni and Campylobacter coli Isolated from Raw Chicken, Pork, and Human Clinical Cases in Korea

Journal of Food Protection ◽

10.4315/0362-028x-73.8.1430 ◽

2010 ◽

Vol 73 (8) ◽

pp. 1430-1437 ◽

Cited By ~ 11

Author(s):

JUN MAN KIM ◽

JOONBAE HONG ◽

WONKI BAE ◽

HYE CHEONG KOO ◽

SO HYUN KIM ◽

...

Keyword(s):

Antibiotic Resistance ◽

Campylobacter Jejuni ◽

Susceptibility Testing ◽

Campylobacter Coli ◽

High Prevalence ◽

Resistance Patterns ◽

High Level ◽

Antibiotic Resistance Patterns ◽

Pork Samples ◽

Level Resistance

The antibiotic resistance patterns and prevalence of the transferable tet(O) plasmid were investigated in Campylobacter jejuni and Campylobacter coli isolates from raw chicken, pork, and humans with clinical campylobacteriosis. A total of 180 C. jejuni and C. coli isolates were identified, and the prevalence rates of C. jejuni and C. coli in raw chicken samples were 83% (83 of 100) and 73% (73 of 100), respectively. Twelve percent (6 of 50) and 10% (5 of 50) of pork samples were contaminated with C. jejuni and C. coli, respectively. Disk diffusion susceptibility testing revealed that the most frequently detected resistance was to tetracycline (92.2%), followed by nalidixic acid (75.6%), ciprofloxacin (65.0%), azithromycin (41.5%), ampicillin (33.3%), and streptomycin (26.1%). Of the C. jejuni and C. coli isolates, 65.7% (n = 109) contained plasmids carrying the tet(O) gene. Six C. jejuni isolates and two C. coli isolates with high-level resistance to tetracycline (MIC = 256 μg/ml) harbored the tet(O) plasmid, which is transferable to other C. jejuni and C. coli isolates. These results demonstrate the presence of an interspecies transferable plasmid containing the tet(O) gene and a high prevalence of antibiotic resistance in Korean Campylobacter isolates and provide an understanding of the antibiotic resistance distribution among Campylobacter species in Korea.

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Host Association of Campylobacter Genotypes Transcends Geographic Variation

Applied and Environmental Microbiology ◽

10.1128/aem.00124-10 ◽

2010 ◽

Vol 76 (15) ◽

pp. 5269-5277 ◽

Cited By ~ 78

Author(s):

Samuel K. Sheppard ◽

Frances Colles ◽

Judith Richardson ◽

Alison J. Cody ◽

Richard Elson ◽

...

Keyword(s):

United Kingdom ◽

The United States ◽

Human Infection ◽

Chicken Meat ◽

Campylobacter Coli ◽

Data Sets ◽

Host Animal ◽

Data Set ◽

The United Kingdom ◽

Retail Chicken Meat

ABSTRACT Genetic attribution of bacterial genotypes has become a major tool in the investigation of the epidemiology of campylobacteriosis and has implicated retail chicken meat as the major source of human infection in several countries. To investigate the robustness of this approach to the provenance of the reference data sets used, a collection of 742 Campylobacter jejuni and 261 Campylobacter coli isolates obtained from United Kingdom-sourced chicken meat was established and typed by multilocus sequence typing. Comparative analyses of the data with those from other isolates sourced from a variety of host animals and countries were undertaken by genetic attribution, genealogical, and population genetic approaches. The genotypes from the United Kingdom data set were highly diverse, yet structured into sequence types, clonal complexes, and genealogical groups very similar to those seen in chicken isolates from the Netherlands, the United States, and Senegal, but more distinct from isolates obtained from ruminant, swine, and wild bird sources. Assignment analyses consistently grouped isolates from different host animal sources regardless of geographical source; these associations were more robust than geographic associations across isolates from three continents. We conclude that, notwithstanding the high diversity of these pathogens, there is a strong signal of association of multilocus genotypes with particular hosts, which is greater than the geographic signal. These findings are consistent with local and international transmission of host-associated lineages among food animal species and provide a foundation for further improvements in genetic attribution.

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Incidence, Antimicrobial Resistance, and Molecular Characteristics of Nontyphoidal Salmonella Including Extended-Spectrum β-Lactamase Producers in Retail Chicken Meat

Journal of Food Protection ◽

10.4315/0362-028x.jfp-15-145 ◽

2015 ◽

Vol 78 (11) ◽

pp. 1932-1937 ◽

Cited By ~ 23

Author(s):

DASOM CHOI ◽

JUNG-WHAN CHON ◽

HONG-SEOK KIM ◽

DONG-HYEON KIM ◽

JONG-SOO LIM ◽

...

Keyword(s):

Multilocus Sequence Typing ◽

Chicken Meat ◽

Molecular Characteristics ◽

Extended Spectrum ◽

Chicken Carcass ◽

Resistance Patterns ◽

Salmonella Virchow ◽

Retail Chicken Meat ◽

Antibiotic Resistance Patterns ◽

Close Genetic Relationship

The present study was undertaken to determine the prevalence of Salmonella in 100 chicken carcass samples from five integrated broiler operation brands in Korea. Serotypes, antibiotic resistance patterns, extended-spectrum β-lactamase (ESBL) genotype, and clonal divergence using multilocus sequence typing of the isolated strains were analyzed. A total of 42 chicken samples were contaminated with nontyphoidal Salmonella (NTS) isolates: 16 isolates (38%) were Salmonella Virchow, 9 (21%) were Salmonella Bareilly, and 8 (19%) were Salmonella Infantis. A multidrug resistance (MDR; resistant to more than three classes of antibiotics) phenotype was observed in 29% of the isolates, which were resistant to five or more classes of antibiotics. The dominant MDR type was resistance to classes of penicillin, cephalosporins, aminoglycosides, quinolones, and tetracyclines. All the MDR isolates were positive for ESBL producers, and all but one (with the CTX-M-1 genotype) had the CTX-M-15 genotype. Multilocus sequence typing of the isolates revealed ST16 as the dominant sequence type; Salmonella Virchow, Salmonella Infantis, and Salmonella Richmond were all ST16, indicating a close genetic relationship between these serovars. This is the first study in Korea showing the CTX-M-1 type of NTS and the prevalence of ESBL-producing strains among NTS isolated from retail chicken meat. Our findings suggest that MDR Salmonella contamination is widely prevalent in retail chicken meat, and consumption of inadequately cooked products could lead to dissemination of NTS, which is hazardous to human health.

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Epidemiology and Outcomes of Nontyphoidal Salmonella Bacteremias from England, 2004 to 2015

Journal of Clinical Microbiology ◽

10.1128/jcm.01189-18 ◽

2018 ◽

Vol 57 (1) ◽

Cited By ~ 1

Author(s):

Shannon Katiyo ◽

Berit Muller-Pebody ◽

Mehdi Minaji ◽

David Powell ◽

Alan P. Johnson ◽

...

Keyword(s):

Antimicrobial Agents ◽

High Morbidity ◽

First Line ◽

Data Set ◽

Content Type ◽

Increased Risk ◽

Resistance Patterns ◽

Prolonged Hospital ◽

Antibiotic Resistance Patterns ◽

Emergence Of Resistance

ABSTRACT Nontyphoidal Salmonella (NTS) bacteremia causes hospitalization and high morbidity and mortality. We linked Gastrointestinal Bacteria Reference Unit (GBRU) data to the Hospital Episode Statistics (HES) data set to study the trends and outcomes of NTS bacteremias in England between 2004 and 2015. All confirmed NTS isolates from blood from England submitted to GBRU between 1 January 2004 and 31 December 2015 were deterministically linked to HES records. Adjusted odds ratios (AOR), proportions, and confidence intervals (CI) were calculated to describe differences in age, sex, antibiotic resistance patterns, and serotypes over time. Males, neonates, and adults above 65 years were more likely to have NTS bacteremia (AOR, 1.54 [95% CI, 1.46 to 1.67]; 2.57 [95% CI, 1.43 to 4.60]; and 3.56 [95% CI, 3.25 to 3.90], respectively). Proportions of bacteremia increased from 1.41% in 2004 to 2.67% in 2015. Thirty-four percent of all blood isolates were resistant to a first-line antibiotic, and 1,397 (56%) blood isolates were linked to an HES record. Of the patients with NTS bacteremia, 969 (69%) had a cardiovascular condition and 155 (12%) patients died, out of which 120 (77%) patients were age 65 years and above. NTS bacteremia mainly affects older people with comorbidities placing them at increased risk of prolonged hospital stay and death. Resistance of invasive NTS to first-line antimicrobial agents appeared to be stable in England, but the emergence of resistance to last-resort antibiotics, such as colistin, requires careful monitoring.

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Antibiotic resistance and residue in chicken, cattle, buffalo and goat meats in different southern districts of Bangladesh

Asian-Australasian Journal of Food Safety and Security ◽

10.3329/aajfss.v5i1.55014 ◽

2021 ◽

Vol 5 (1) ◽

pp. 19-26

Author(s):

Bidyut Matubber ◽

Farzana Islam Rume ◽

Mohammad Enamul Hoque Kayesh ◽

Mohammad Mahfuzur Rahman ◽

Mohammad Rohul Amin ◽

...

Keyword(s):

Antibiotic Resistance ◽

Chicken Meat ◽

Human Consumption ◽

Antibiotic Residues ◽

Salmonella Spp ◽

Resistance Patterns ◽

Cattle Meat ◽

Meat Samples ◽

Controlling Measures ◽

Antibiotic Resistance Patterns

The presence of antibiotic residue in chicken and animal meats is a serious threat to human health due to its harmful effects. This study aimed at identifying the antibiotic resistance patterns of the isolates as well as antibiotic residues in chicken, cattle, buffalo and goat meats in different southern districts of Bangladesh. A total of 205 meat samples, including 70 chicken meat, 60 cattle meat, 50 buffalo meat and 25 goat meat were aseptically collected and analysed for the detection of antibiotic residues by thin layer chromatography and the isolates obtained from these samples were subjected to antibiogram study against 16 commonly used antibiotics. The isolates found in this study were Staphylococcus spp., Streptococcus spp., Escherichia coli, and Salmonella spp. and their prevalence were 37.5% (77/205), 22.1% (48/205), 29.7% (61/205), 8.7% (19/205), respectively. The isolates showed different degrees of sensitivity to the antibiotics used in the study. The most resistant phenotype was against cefradine, amoxicillin, penicillin, oxytetracycline, erythromycin, and enrofloxacin. 18.5% (38/205) meat samples were found to be positive for antibiotic residues and the highest prevalence was observed in chicken meat compared to other meat types. Overall, the findings of the study suggest that it is important to take controlling measures for the emergence of antibiotic resistance and also for ensuring healthy meats for human consumption. Asian Australas. J. Food Saf. Secur. 2021, 5 (1), 19-26

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Detection of Campylobacter jejuni and Campylobacter coli from Broiler Chicken–Related Samples Using BAX PCR and Conventional International Organization for Standardization Culture

Journal of Food Protection ◽

10.4315/0362-028x-71.4.835 ◽

2008 ◽

Vol 71 (4) ◽

pp. 835-838 ◽

Cited By ~ 9

Author(s):

LISA K. WILLIAMS ◽

ALISDAIR MCMEECHAN ◽

TAMSIN BAALHAM ◽

LAURA WARD ◽

TOM J. HUMPHREY ◽

...

Keyword(s):

Campylobacter Jejuni ◽

Broiler Chicken ◽

Culture Method ◽

International Organization ◽

Campylobacter Coli ◽

Poultry Production ◽

Production Chain ◽

Enrichment Cultures ◽

International Organization For Standardization ◽

Campylobacter Spp

In this study, the conventional International Organization for Standardization (ISO) culture method was compared with the DuPont Qualicon BAX system, a high-throughput, rapid molecular assay that can be used to detect several bacterial species, including Campylobacter jejuni and Campylobacter coli in diverse sample types. Standard enrichment culture is a time-consuming process, taking up to 6 days to obtain a confirmed result. Rapid molecular assays have been developed that provide results within 24 h. Naturally contaminated samples from the poultry production chain were examined for the presence of Campylobacter spp. Samples from broiler chicken ceca (n = 100), fresh chicken carcass rinses (n = 60), and bootsocks (gauze sock walked through a broiler chicken house; n = 50) were enriched according to the ISO 10272 method in Bolton broth specifically designed to detect Campylobacter spp. in complex sample types. Samples were enriched without blood for use with the BAX system using the Campylobacter BAX kits for the detection of C. jejuni and C. coli. Samples also were directly plated onto modified charcoal cefperazone deoxycholate agar, and results were compared with those from the enriched samples for the ability to detect Campylobacter spp. Campylobacter spp. were isolated from 49% of samples with conventional enrichment cultures, from 48% with direct culture, from 68% with the BAX system and enrichment cultures, and from 62% with the BAX system used directly with samples. Overall, the BAX system detected more positive samples than did the conventional culture method and is an effective methodology for the rapid and reliable detection of Campylobacter spp. from diverse sample types.

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Prevalence and susceptibility to antibiotics from Campylobacter jejuni and Campylobacter coli isolated from chicken meat in southern Benin, West Africa

BMC Research Notes ◽

10.1186/s13104-020-05150-x ◽

2020 ◽

Vol 13 (1) ◽

Author(s):

Sylvain Daton Kouglenou ◽

Alidehou Jerrold Agbankpe ◽

Victorien Dougnon ◽

Armando Djiyou Djeuda ◽

Esther Deguenon ◽

...

Keyword(s):

West Africa ◽

Campylobacter Jejuni ◽

Chicken Meat ◽

Campylobacter Coli ◽

Southern Benin

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Genetics behind the Biosynthesis of Nonulosonic Acid-Containing Lipooligosaccharides inCampylobacter coli

Journal of Bacteriology ◽

10.1128/jb.00759-18 ◽

2019 ◽

Vol 201 (8) ◽

Author(s):

Alejandra Kolehmainen ◽

Mirko Rossi ◽

Jacek Stupak ◽

Jianjun Li ◽

Michel Gilbert ◽

...

Keyword(s):

Campylobacter Jejuni ◽

Foodborne Pathogen ◽

Campylobacter Coli ◽

Strong Impact ◽

Large Set ◽

Content Type ◽

Donor And Acceptor ◽

Nonulosonic Acid ◽

Acute Polyneuropathy

ABSTRACTCampylobacter jejuniandCampylobacter coliare the most common causes of bacterial gastroenteritis in the world. Ganglioside mimicry byC. jejunilipooligosaccharide (LOS) is the triggering factor of Guillain-Barré syndrome (GBS), an acute polyneuropathy. Sialyltransferases from glycosyltransferase family 42 (GT-42) are essential for the expression of ganglioside mimics inC. jejuni. Recently, two novel GT-42 genes,cstIVandcstV, have been identified inC. coli. Despite being present in ∼11% of currently availableC. coligenomes, the biological role ofcstIVandcstVis unknown. In the present investigation, mutation studies with two strains expressing eithercstIVorcstVwere performed and mass spectrometry was used to investigate differences in the chemical composition of LOS. Attempts were made to identify donor and acceptor molecules usingin vitroactivity tests with recombinant GT-42 enzymes. Here we show that CstIV and CstV are involved inC. coliLOS biosynthesis. In particular,cstVis associated with LOS sialylation, whilecstIVis linked to the addition of a diacetylated nonulosonic acid residue.IMPORTANCEDespite the fact thatCampylobacter colia major foodborne pathogen, its glycobiology has been largely neglected. The genetic makeup of theC. colilipooligosaccharide biosynthesis locus was largely unknown until recently.C. coliharbors a large set of genes associated with lipooligosaccharide biosynthesis, including genes for several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide inCampylobacter jejuni. In the present study,C. coliwas found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids. These findings have a strong impact on our understanding ofC. coliecology, host-pathogen interaction, and pathogenesis.

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Active Packaging of Immobilized Zinc Oxide Nanoparticles Controls Campylobacter jejuni in Raw Chicken Meat

Applied and Environmental Microbiology ◽

10.1128/aem.01195-20 ◽

2020 ◽

Vol 86 (22) ◽

Author(s):

Mohammed J. Hakeem ◽

Jinsong Feng ◽

Azadeh Nilghaz ◽

Luyao Ma ◽

Hwai Chuin Seah ◽

...

Keyword(s):

Zinc Oxide ◽

Shelf Life ◽

Campylobacter Jejuni ◽

Zinc Oxide Nanoparticles ◽

Chicken Meat ◽

Oxide Nanoparticles ◽

Dependent Manner ◽

Zno Nps ◽

Content Type ◽

Active Food Packaging

ABSTRACT Zinc oxide nanoparticles (ZnO NPs) are regarded as a safe and stable antimicrobial that can inactivate bacteria by several potential working mechanisms. We aimed to incorporate ZnO NPs into packaging material to control Campylobacter in raw chicken meat. ZnO NPs were first incorporated into three-dimensional (3D) paper tubes to identify the lethal concentration against Campylobacter jejuni, which was selected as the working concentration to develop 2D functionalized absorbing pads by an ultrasound-assisted dipping technique. The functionalized pad was placed underneath raw chicken meat to inactivate C. jejuni and the predominant chicken microbiota at 4°C within 8 days of storage. Immobilized ZnO NPs at 0.856 mg/cm2 reduced C. jejuni from ∼4 log CFU/25 g raw chicken meat to an undetectable level after 3 days of storage. Analysis by inductively coupled plasma-optical emission spectroscopy showed that the Zn level increased from 0.02 to 0.17 mg/cm2 in treated raw chicken meat. Scanning electron microscopy validated the absence of nanoparticle migration onto raw chicken meat after treatment. Inactivation of C. jejuni was associated with the increase of lactic acid produced by Lactobacillus in raw chicken meat in a pH-dependent manner. Less than 5% of Zn2+ was released from ZnO NPs at neutral pH, while up to 88% was released when the pH was <3.5 within 2 days. Whole-transcriptome sequencing (RNA-Seq) analysis demonstrated a broad effect of ZnO NPs on genes involved in various cellular developmental processes as annotated by gene ontology. Taken together, the results indicate that functionalized absorbing pads inactivated C. jejuni in raw chicken meat by immobilized ZnO NPs along with the controllable released Zn2+. IMPORTANCE Prevalence of Campylobacter in raw poultry remains a major food microbiological safety challenge. Novel mitigation strategies are required to ensure the safety and quality of poultry products. Active food packaging can control pathogens without directly adding antimicrobials into the food matrix and extend the food’s shelf life. The functionalized absorbing pad with ZnO NPs developed in this study was able to inactivate C. jejuni in raw chicken meat and keep the meat free from C. jejuni contamination during shelf life without any observed migration of nanoparticles. The controllable conversion of immobilized ZnO NPs to free Zn2+ makes this approach safe and eco-friendly and paves the way for developing a novel intervention strategy for other high-risk foods. Our study applied nanotechnology to exploit an effective approach for Campylobacter control in raw chicken meat products.

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