scholarly journals Age-associated DNA methylation changes in immune genes, histone modifiers and chromatin remodeling factors within 5 years after birth in human blood leukocytes

2015 ◽  
Vol 7 (1) ◽  
Author(s):  
Nathalie Acevedo ◽  
Lovisa E Reinius ◽  
Morana Vitezic ◽  
Vittorio Fortino ◽  
Cilla Söderhäll ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Chromatin Remodeling ◽  
Human Blood ◽  
Blood Leukocytes ◽  
Immune Genes ◽  
Histone Modifiers

scholarly journals Profile of copper-associated DNA methylation and its association with incident acute coronary syndrome

2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Pinpin Long ◽  
Qiuhong Wang ◽  
Yizhi Zhang ◽  
Xiaoyan Zhu ◽  
Kuai Yu ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Acute Coronary Syndrome ◽  
Methylation Level ◽  
Meta Analysis ◽  
Regulation Of Gene Expression ◽  
Density Lipoprotein ◽  
Blood Leukocytes ◽  
Coronary Syndrome ◽  
A Genome ◽  
Increased Risk

Abstract Background Acute coronary syndrome (ACS) is a cardiac emergency with high mortality. Exposure to high copper (Cu) concentration has been linked to ACS. However, whether DNA methylation contributes to the association between Cu and ACS is unclear. Methods We measured methylation level at > 485,000 cytosine-phosphoguanine sites (CpGs) of blood leukocytes using Human Methylation 450 Bead Chip and conducted a genome-wide meta-analysis of plasma Cu in a total of 1243 Chinese individuals. For plasma Cu-related CpGs, we evaluated their associations with the expression of nearby genes as well as major cardiovascular risk factors. Furthermore, we examined their longitudinal associations with incident ACS in the nested case-control study. Results We identified four novel Cu-associated CpGs (cg20995564, cg18608055, cg26470501 and cg05825244) within a 5% false discovery rate (FDR). DNA methylation level of cg18608055, cg26470501, and cg05825244 also showed significant correlations with expressions of SBNO2, BCL3, and EBF4 gene, respectively. Higher DNA methylation level at cg05825244 locus was associated with lower high-density lipoprotein cholesterol level and higher C-reactive protein level. Furthermore, we demonstrated that higher cg05825244 methylation level was associated with increased risk of ACS (odds ratio [OR], 1.23; 95% CI 1.02–1.48; P = 0.03). Conclusions We identified novel DNA methylation alterations associated with plasma Cu in Chinese populations and linked these loci to risk of ACS, providing new insights into the regulation of gene expression by Cu-related DNA methylation and suggesting a role for DNA methylation in the association between copper and ACS.

scholarly journals Blood-Based Detection of Colorectal Cancer Using Cancer-Specific DNA Methylation Markers

Diagnostics ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 51
Author(s):  
Nam-Yun Cho ◽  
Ji-Won Park ◽  
Xianyu Wen ◽  
Yun-Joo Shin ◽  
Jun-Kyu Kang ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Dna Methylation ◽  
Sensitivity And Specificity ◽  
Stage Iv ◽  
High Sensitivity ◽  
Liquid Biopsies ◽  
Blood Leukocytes ◽  
Marker Panel ◽  
A Genome ◽  
Methylight Assay

Cancer tissues have characteristic DNA methylation profiles compared with their corresponding normal tissues that can be utilized for cancer diagnosis with liquid biopsy. Using a genome-scale DNA methylation approach, we sought to identify a panel of DNA methylation markers specific for cell-free DNA (cfDNA) from patients with colorectal cancer (CRC). By comparing DNA methylomes between CRC and normal mucosal tissues or blood leukocytes, we identified eight cancer-specific methylated loci (ADGRB1, ANKRD13, FAM123A, GLI3, PCDHG, PPP1R16B, SLIT3, and TMEM90B) and developed a five-marker panel (FAM123A, GLI3, PPP1R16B, SLIT3, and TMEM90B) that detected CRC in liquid biopsies with a high sensitivity and specificity with a droplet digital MethyLight assay. In a set of cfDNA samples from CRC patients (n = 117) and healthy volunteers (n = 60), a panel of five markers on the platform of the droplet digital MethyLight assay detected stages I–III and stage IV CRCs with sensitivities of 45.9% and 95.7%, respectively, and a specificity of 95.0%. The number of detected markers was correlated with the cancer stage, perineural invasion, lymphatic emboli, and venous invasion. Our five-marker panel with the droplet digital MethyLight assay showed a high sensitivity and specificity for the detection of CRC with cfDNA samples from patients with metastatic CRC.

scholarly journals Differential DNA Methylation in Purified Human Blood Cells: Implications for Cell Lineage and Studies on Disease Susceptibility

PLoS ONE ◽  
2012 ◽  
Vol 7 (7) ◽  
pp. e41361 ◽  
Author(s):  
Lovisa E. Reinius ◽  
Nathalie Acevedo ◽  
Maaike Joerink ◽  
Göran Pershagen ◽  
Sven-Erik Dahlén ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Human Blood ◽  
Blood Cells ◽  
Disease Susceptibility ◽  
Cell Lineage ◽  
Human Blood Cells

scholarly journals ANALYSIS OF THE ASSOCIATION OF THE METHYLATION LEVELS OF MIR10B AND MIR21 GENES IN BLOOD LEUKOCYTES WITH ADVANCED CAROTID ATHEROSCLEROSIS

2018 ◽  
Vol 33 (2) ◽  
pp. 77-82
Author(s):  
Iu. A. Koroleva ◽  
A. A. Zarubin ◽  
A. V. Markov ◽  
A. N. Kazancev ◽  
O. L. Barbarash ◽  
...  
Keyword(s):  
Risk Factors ◽  
Dna Methylation ◽  
Methylation Level ◽  
Carotid Atherosclerosis ◽  
Gene Promoter ◽  
Control Group ◽  
Coding Region ◽  
Blood Leukocytes ◽  
Atherosclerosis Risk Factors

Complications of atherosclerosis remain the leading cause of morbidity and mortality worldwide. MiRNAs are short regulatory molecules that are involved in all processes of pathogenesis. Expression of miRNAs is regulated by DNA methylation. Methylation and/or expression of MIR10B and MIR21 genes are known to vary in atherosclerotic tissues of the arteries, but there is no data about the changes in the methylation levels of these genes in blood leukocytes and their association with atherosclerosis risk factors.Objective.To evaluate the association of methylation levels of MIR10B and MIR21 genes in the blood leukocytes with risk factors and pathogenetically significant traits of carotid atherosclerosis.Material and Methods. DNA for the study was extracted from the samples of blood leukocytes of 122 patients with advanced carotid atherosclerosis as well as from blood leukocytes of 135 individuals in the control group. The DNA methylation level was analyzed by bisulfite pyrosequencing.Results.The methylation level of the MIR10B and MIR21 genes in leukocytes of patients with atherosclerosis is higher than in the leukocytes of the control group. In leukocytes of patients with carotid atherosclerosis the methylation level of the MIR21 gene promoter was correlated with type 2 diabetes and serum cholesterol level, and the methylation level of the coding region of the MIR10B gene was correlated with smoking.Conclusions.The level of DNA methylation in the regions of MIR10B and MIR21 genes in blood leukocytes is associated with the risk of advanced atherosclerosis of the carotid arteries. 

The Interaction Of Bifidobacteria With Human Blood Leukocytes

2014 ◽  
Vol 133 (2) ◽  
pp. AB57
Author(s):  
Leonid P. Titov ◽  
A.S. Murashko ◽  
Andrei Y. Hancharou ◽  
N.A. Golovnyova ◽  
E.I. Kolomiets ◽  
...  
Keyword(s):  
Human Blood ◽  
Blood Leukocytes

Abstract 24: Prenatal Socioeconomic Position is Associated with Adipose Tissue DNA Methylation in Women and Not Men

Circulation ◽  
2014 ◽  
Vol 129 (suppl_1) ◽  
Author(s):  
Golareh Agha ◽  
Andres E Houseman ◽  
Karl T Kelsey ◽  
Charles B Eaton ◽  
Stephen L Buka ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Adipose Tissue ◽  
New England ◽  
Early Life ◽  
Blood Leukocytes ◽  
Early Life Adversity ◽  
Smoking During Pregnancy ◽  
Socioeconomic Index ◽  
Affected Tissue ◽  
Subcutaneous Adipose

RATIONALE: Adiposity is a major cardiovascular risk factor, suggesting an important role for adipose tissue in development of cardiovascular outcomes. There is evidence that early life adversity has a lasting impact on the development of adiposity, particularly in women. In utero exposure to famine was related to adulthood adiposity in women but not men, and associations between early life socioeconomic adversity and adulthood adiposity is established in women but less so in men. There is interest in epigenetic mechanisms by which early life adversity may program risk for adiposity, and utilizing directly affected tissue such as adipose tissue is ideal for investigating such mechanisms. Objective: To determine whether prenatally-assessed socioeconomic index (SEI) is associated with adulthood genome-wide DNA methylation in blood and adipose tissue, and whether associations differ between men and women. Methods: Participants (aged 44-50 y) were from the New England Family Study birth cohort, born in Providence, RI. Of 400 participants assessed during 2010-2011, a representative subsample of 106 participants (68 women, 38 men) was selected for DNA methylation analyses. SEI was measured prenatally as a composite numerical score, using a weighted percentile of both parents’ educational attainment, occupation, and income relative to the US population. DNA methylation in subcutaneous adipose tissue and peripheral blood leukocytes was evaluated using the Infinium HumanMethylation450K BeadChip. Results: Prenatal SEI was associated with adipose tissue DNA methylation in women (permutation-based omnibus p-values <0.001), but not men or the pooled sample. Associations in women were not attenuated after adjustment for race, current smoking, or mother’s smoking during pregnancy. Prenatal SEI was not related to blood DNA methylation. Conclusion: Results provide mechanistic insight on the association between early life adversity and adulthood adiposity, which is seen mainly in women.

Human Blood Leukocytes

1973 ◽  
pp. 58-61
Author(s):  
Paul S. Moorhead
Keyword(s):  
Human Blood ◽  
Blood Leukocytes
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