scholarly journals Sex-dependent differences in the secretome of human endothelial cells

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Maria Grazia Cattaneo ◽  
Cristina Banfi ◽  
Maura Brioschi ◽  
Donatella Lattuada ◽  
Lucia M. Vicentini
Keyword(s):  
Endothelial Cells ◽  
Secreted Proteins ◽  
Mrna Levels ◽  
Preclinical Research ◽  
Male And Female ◽  
Human Male ◽  
Pharmacological Targets ◽  
Endothelial Response ◽  
Significant Enrichment ◽  
Male Specific

Abstract Background Cellular sex has rarely been considered as a biological variable in preclinical research, even when the pathogenesis of diseases with predictable sex differences is studied. In this perspective, proteomics, and “omics” approaches in general, can provide powerful tools to obtain comprehensive cellular maps, thus favoring the discovery of still unknown sex-biased physio-pathological mechanisms. Methods We performed proteomic and Gene Ontology (GO) analyses of the secretome from human serum-deprived male and female endothelial cells (ECs) followed by ELISA validation. Apoptosis was detected by FACS and Western blot techniques and efferocytosis through the ability of the macrophage cell line RAW 264.7 to engulf apoptotic ECs. PTX3 mRNA levels were measured by RT-qPCR. Results Proteomic and GO analyses of the secretome from starved human male and female ECs demonstrated a significant enrichment in proteins related to cellular responses to stress and to the regulation of apoptosis in the secretome of male ECs. Accordingly, a higher percentage of male ECs underwent apoptosis in response to serum deprivation in comparison with female ECs. Among the secreted proteins, we reliably found higher levels of PTX3 in the male EC secretome. The silencing of PTX3 suggested that male ECs were dependent on its expression to properly carry out the efferocytotic process. At variance, female EC efferocytosis seemed to be independent on PTX3 expression. Conclusions Our results demonstrated that serum-starved male and female ECs possess different secretory phenotypes that might take part in the sex-biased response to cellular stress. We identified PTX3 as a crucial player in the male-specific endothelial response to an apoptotic trigger. This novel and sex-related role for secreted proteins, and mainly for PTX3, may open the way to the discovery of still unknown sex-specific mechanisms and pharmacological targets for the prevention and treatment of endothelial dysfunction at the onset of atherosclerosis and cardiovascular disease.

scholarly journals Sex-dependent differences in the secretome of human endothelial cells

2020 ◽  
Author(s):  
Maria Grazia Cattaneo ◽  
Cristina Banfi ◽  
Maura Brioschi ◽  
Donatella Lattuada ◽  
Lucia M. Vicentini
Keyword(s):  
Endothelial Cells ◽  
Secreted Proteins ◽  
Mrna Levels ◽  
Preclinical Research ◽  
Male And Female ◽  
Human Male ◽  
Pharmacological Targets ◽  
Endothelial Response ◽  
Significant Enrichment ◽  
Male Specific

Abstract Background: Cellular sex has been rarely considered as a biological variable in preclinical research, even when the pathogenesis of diseases with predictable sex differences is studied. In this perspective, proteomics, and ‘omics approaches in general, can provide powerful tools to obtain comprehensive cellular maps, thus favoring the discovery of still unknown sex-biased physio-pathological mechanisms.Methods: We performed proteomic and gene ontology (GO) analyses of secretome from human serum-deprived male and female endothelial cells (ECs) followed by ELISA validation. Apoptosis was detected by FACS and western blot techniques, and efferocytosis through the ability of the macrophage cell line RAW-264.7 to engulf apoptotic ECs. PTX3 mRNA levels were measured by RT-qPCR. Results: Proteomic and GO analyses of the secretome from starved human male and female ECs demonstrated a significant enrichment in proteins related to cellular responses to stress and to the regulation of apoptosis in the secretome of male ECs. Accordingly, a higher percentage of male ECs underwent apoptosis in response to serum deprivation in comparison to female ECs. Among the secreted proteins, we reliably found higher levels of PTX3 in the male EC secretome. The silencing of PTX3 suggests that male ECs were dependent on its expression to properly carry out the efferocytotic process. At variance, female EC efferocytosis seems to be independent on PTX3 expression. Conclusions: Our results demonstrated that serum-starved male and female ECs possess different secretory phenotypes that might take part in the sex-biased response to cellular stress. We identified PTX3 as a crucial player in the male-specific endothelial response to an apoptotic trigger. This novel and sex-related role for secreted proteins, and mainly for PTX3, may open the way to the discovery of still unknown sex-specific mechanisms and pharmacological targets for the prevention and treatment of endothelial dysfunction at the onset of atherosclerosis and cardiovascular disease.

scholarly journals Sex-dependent differences in the secretome of human endothelial cells

2020 ◽  
Author(s):  
Maria Grazia Cattaneo ◽  
Cristina Banfi ◽  
Maura Brioschi ◽  
Donatella Lattuada ◽  
Lucia M. Vicentini
Keyword(s):  
Endothelial Cells ◽  
Secreted Proteins ◽  
Mrna Levels ◽  
Preclinical Research ◽  
Male And Female ◽  
Human Male ◽  
Pharmacological Targets ◽  
Endothelial Response ◽  
Significant Enrichment ◽  
Male Specific

Abstract Background: Cellular sex has rarely been considered as a biological variable in preclinical research, even when the pathogenesis of diseases with predictable sex differences is studied. In this perspective, proteomics, and ‘omics’ approaches in general, can provide powerful tools to obtain comprehensive cellular maps, thus favoring the discovery of still unknown sex-biased physio-pathological mechanisms.Methods: We performed proteomic and gene ontology (GO) analyses of secretome from human serum-deprived male and female endothelial cells (ECs) followed by ELISA validation. Apoptosis was detected by FACS and Western blot techniques, and efferocytosis through the ability of the macrophage cell line RAW-264.7 to engulf apoptotic ECs. PTX3 mRNA levels were measured by RT-qPCR. Results: Proteomic and GO analyses of the secretome from starved human male and female ECs demonstrated a significant enrichment in proteins related to cellular responses to stress and to the regulation of apoptosis in the secretome of male ECs. Accordingly, a higher percentage of male ECs underwent apoptosis in response to serum deprivation in comparison to female ECs. Among the secreted proteins, we reliably found higher levels of PTX3 in the male EC secretome. The silencing of PTX3 suggested that male ECs were dependent on its expression to properly carry out the efferocytotic process. At variance, female EC efferocytosis seemed to be independent on PTX3 expression. Conclusions: Our results demonstrated that serum-starved male and female ECs possess different secretory phenotypes that might take part in the sex-biased response to cellular stress. We identified PTX3 as a crucial player in the male-specific endothelial response to an apoptotic trigger. This novel and sex-related role for secreted proteins, and mainly for PTX3, may open the way to the discovery of still unknown sex-specific mechanisms and pharmacological targets for the prevention and treatment of endothelial dysfunction at the onset of atherosclerosis and cardiovascular disease.

scholarly journals Sex-Dependent Differences in the Secretome of Human Endothelial Cells

2020 ◽  
Author(s):  
Maria Grazia Cattaneo ◽  
Cristina Banfi ◽  
Maura Brioschi ◽  
Donatella Lattuada ◽  
Lucia M. Vicentini
Keyword(s):  
Endothelial Cells ◽  
Secreted Proteins ◽  
Human Endothelial Cells ◽  
Preclinical Research ◽  
Male And Female ◽  
Human Male ◽  
Pharmacological Targets ◽  
Endothelial Response ◽  
Significant Enrichment ◽  
Male Specific

Abstract Background: Cellular sex has been rarely considered as a biological variable in preclinical research, even when the pathogenesis of diseases with predictable sex differences is studied. In this perspective, proteomics, and ‘omics approaches in general, can provide powerful tools to obtain comprehensive cellular maps, thus favoring the discovery of still unknown sex-biased physio-pathological mechanisms.Methods: We performed proteomic and gene ontology (GO) analyses of secretome from human serum-deprived male and female endothelial cells (ECs) followed by ELISA validation. Apoptosis was detected by FACS and western blot techniques, and efferocytosis through the ability of the macrophage cell line RAW-264.7 to engulf apoptotic ECs. Protein expression and silencing efficacy were assessed by RT-qPCR. Results: Proteomic and GO analyses of the secretome from starved human male and female ECs demonstrated a significant enrichment in proteins related to cellular responses to stress and to the regulation of apoptosis in the secretome of male ECs. Accordingly, a higher percentage of male ECs underwent apoptosis in response to serum deprivation in comparison to female ECs. Among the secreted proteins, we reliably found higher levels of PTX3 in the male EC secretome. The silencing of PTX3 proved that male ECs were dependent on its expression to properly carry out the efferocytotic process. At variance, female EC efferocytosis was independent of PTX3 expression. Conclusions: Our results demonstrated that serum-starved male and female ECs possess different secretory phenotypes that might take part in the sex-biased response to cellular stress. We identified PTX3 as a crucial player in the male-specific endothelial response to an apoptotic trigger. This novel and sex-related role for secreted proteins, and mainly for PTX3, may open the way to the discovery of still unknown sex-specific mechanisms and pharmacological targets for the prevention and treatment of endothelial dysfunction at the onset of atherosclerosis and cardiovascular disease.

scholarly journals Fatty acids rather than hormones restore in vitro angiogenesis in human male and female endothelial cells cultured in charcoal-stripped serum

PLoS ONE ◽  
2017 ◽  
Vol 12 (12) ◽  
pp. e0189528 ◽  
Author(s):  
Claudia Vanetti ◽  
Francesco Bifari ◽  
Lucia M. Vicentini ◽  
Maria Grazia Cattaneo
Keyword(s):  
Fatty Acids ◽  
Endothelial Cells ◽  
Male And Female ◽  
Human Male ◽  
In Vitro Angiogenesis ◽  
Cells Cultured

scholarly journals Sex Differences in Escalated Methamphetamine Self-Administration and Altered Gene Expression Associated With Incubation of Methamphetamine Seeking

2019 ◽  
Vol 22 (11) ◽  
pp. 710-723 ◽  
Author(s):  
Atul P Daiwile ◽  
Subramaniam Jayanthi ◽  
Bruce Ladenheim ◽  
Michael T McCoy ◽  
Christie Brannock ◽  
...  
Keyword(s):  
Sex Differences ◽  
Nucleus Accumbens ◽  
Fixed Ratio ◽  
Female Rats ◽  
Male Rats ◽  
Mrna Levels ◽  
Self Administration ◽  
Male And Female ◽  
Orexin Receptors ◽  
Male And Female Rats

Abstract Background Methamphetamine (METH) use disorder is prevalent worldwide. There are reports of sex differences in quantities of drug used and relapses to drug use among individuals with METH use disorder. However, the molecular neurobiology of these potential sex differences remains unknown. Methods We trained rats to self-administer METH (0. 1 mg/kg/infusion, i.v.) on an fixed-ratio-1 schedule for 20 days using two 3-hour daily METH sessions separated by 30-minute breaks. At the end of self-administration training, rats underwent tests of cue-induced METH seeking on withdrawal days 3 and 30. Twenty-four hours later, nucleus accumbens was dissected and then used to measure neuropeptide mRNA levels. Results Behavioral results show that male rats increased the number of METH infusions earlier during self-administration training and took more METH than females. Both male and female rats could be further divided into 2 phenotypes labeled high and low takers based on the degree of escalation that they exhibited during the course of the METH self-administration experiment. Both males and females exhibited incubation of METH seeking after 30 days of forced withdrawal. Females had higher basal mRNA levels of dynorphin and hypocretin/orexin receptors than males, whereas males expressed higher vasopressin mRNA levels than females under saline and METH conditions. Unexpectedly, only males showed increased expression of nucleus accumbens dynorphin after METH self-administration. Moreover, there were significant correlations between nucleus accumbens Hcrtr1, Hcrtr2, Crhr2, and Avpr1b mRNA levels and cue-induced METH seeking only in female rats. Conclusion Our results identify some behavioral and molecular differences between male and female rats that had self-administered METH. Sexual dimorphism in responses to METH exposure should be considered when developing potential therapeutic agents against METH use disorder.

Thrombospondin-1 Modulates the Angiogenic Phenotype of Human Cerebral Arteriovenous Malformation Endothelial Cells

Neurosurgery ◽  
2011 ◽  
Vol 68 (5) ◽  
pp. 1342-1353 ◽  
Author(s):  
Christopher J. Stapleton ◽  
Don L. Armstrong ◽  
Raphael Zidovetzki ◽  
Charles Y. Liu ◽  
Steven L. Giannotta ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Arteriovenous Malformation ◽  
Immunosorbent Assay ◽  
Cerebral Arteriovenous Malformation ◽  
Enzyme Linked Immunosorbent Assay ◽  
Boyden Chamber ◽  
Mrna Levels ◽  
Tubule Formation ◽  
Thrombospondin 1 ◽  
And Migration

Abstract BACKGROUND: The management of cerebral arteriovenous malformation (AVM) is challenging, and invasive therapies place vital intracranial structures at risk of injury. The development of noninvasive, pharmacologic approaches relies on identifying factors that mediate key angiogenic processes. Previous studies indicate that endothelial cells (ECs) derived from cerebral AVM (AVM-ECs) are distinct from control brain ECs with regard to important angiogenic characteristics. OBJECTIVE: To determine whether thrombospondin-1 (TSP-1), a potent angiostatic factor, regulates critical angiogenic features of AVM-ECs and to identify factors that modulate TSP-1 production in AVM-ECs. METHODS: EC proliferation, migration, and tubule formation were evaluated with bromodeoxyuridine incorporation, Boyden chamber, and Matrigel studies, respectively. TSP-1 and inhibitor of DNA binding/differentiation 1 (Id1) mRNA levels were quantified with microarray and quantitative real-time polymerase chain reaction analyses. TSP-1 protein expression was measured using Western blotting, immunohistochemical, and enzyme-linked immunosorbent assay techniques. The mechanistic link between Id1 and TSP-1 was established through small interfering RNA-mediated knockdown of Id1 in AVM-ECs followed by Western blot and enzyme-linked immunosorbent assay experiments assessing TSP-1 production. RESULTS: AVM-ECs proliferate faster, migrate more quickly, and form disorganized tubules compared with brain ECs. TSP-1 is significantly down-regulated in AVM-ECs. The addition of TSP-1 to AVM-EC cultures normalizes the rate of proliferation and migration and the efficiency of tubule formation, whereas brain ECs are unaffected. Id1 negatively regulates TSP-1 expression in AVM-ECs. CONCLUSION: These data highlight a novel role for TSP-1 in the pathobiology of AVM angiogenesis and provide a context for its use in the clinical management of brain AVMs.

Comparative proteomics of human male and female tears by two-dimensional electrophoresis

2011 ◽  
Vol 92 (6) ◽  
pp. 454-463 ◽  
Author(s):  
Sivagnanam Ananthi ◽  
Ramachandran Sarojini Santhosh ◽  
Murugesan Valar Nila ◽  
Namperumalsamy Venkatesh Prajna ◽  
Prajna Lalitha ◽  
...  
Keyword(s):  
Comparative Proteomics ◽  
Two Dimensional ◽  
Male And Female ◽  
Two Dimensional Electrophoresis ◽  
Human Male ◽  
Dimensional Electrophoresis

Hormonal control of vitellogenin mRNA levels in the male and female housefly, Musca domestica

1991 ◽  
Vol 37 (5) ◽  
pp. 383-390 ◽  
Author(s):  
Noriaki Agui ◽  
Toru Shimada ◽  
Susumu Izumi ◽  
Shiro Tomino
Keyword(s):  
Musca Domestica ◽  
Hormonal Control ◽  
Mrna Levels ◽  
Male And Female ◽  
Vitellogenin Mrna

Salsolaius gen. nov. a new genus of Apalochrini (Coleoptera, Melyridae, Malachiinae) from the salt Lake Way of Western Australia

Zootaxa ◽  
2021 ◽  
Vol 5082 (4) ◽  
pp. 393-400
Author(s):  
ZHENHUA LIU ◽  
ADAM ŚLIPIŃSKI ◽  
HONG PANG
Keyword(s):  
Western Australia ◽  
Type Species ◽  
New Genus ◽  
Salt Lake ◽  
Salt Lakes ◽  
Male And Female ◽  
Male Specific

Apalochrini comprises nearly half of the genera of Australian Melyridae, which are all recognized by male specific characters, and are commonly found on grasses, flowers and riverside or seashore rocks. Here we describe a new genus Salsolaius gen. nov. from Lake Way of Western Australia, representing the first known genus of Australian Melyridae inhabitating in salt lakes. The new genus can be easily distinguished by asymmetrically biserrate antennae and exposed apical abdomen from above in both male and female, the former characters is firstly found in Melyridae. Consequently, Salsolaius biserratus sp. nov. was described as the type species of this genus. An updated key to genera of Australian Apalochrini is provided.  

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