scholarly journals Method comparison of Particle Enhanced Immunoturbidimetry (PEIT) with High Performance Liquid Chromatography (HPLC) for glycated hemoglobin (HbA1c) analysis

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Shabnam Dildar ◽  
Sheharbano Imran ◽  
Farah Naz
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Coefficient Of Variation ◽  
High Performance ◽  
Evaluation Method ◽  
Total Error ◽  
Method Comparison ◽  
Systemic Error ◽  
Hplc Technique ◽  
The Difference

Abstract Background and objective High Performance Liquid Chromatography (HPLC) technique is considered as a gold standard for HbA1c analysis however all laboratories cannot adopt it due to certain limitations. Our aim was to compare Particle Enhanced Immunoturbidimetry (PEIT) method with High Performance Liquid Chromatography (HPLC) for HbA1c analysis. Method All blood samples were analyzed by HPLC assay on a Bio-Rad D-10 analyzer and PEIT on an Erba XL-200 analyzer. Precision studies were undertaken and Coefficient of Variation (%CV) calculated. Systemic Error (SE), Random Error (RE) and Total Error (TEcalc) were obtained. The Total Allowable Error (TEa) set by the National Glycohemoglobin Standardization Program (NGSP) for HbA1c is 6%.The acceptable evaluation method is where TEcalc is less than TEa. Results The Precision studies were satisfactory with Coefficient of Variation (%CV) being less than 4% for both techniques. Mean HbA1c levels were slightly higher from HPLC than PEIT 9.07 ± 2.23% and 8.93 ± 2.10% respectively, although the difference was minimal. RE was 1.41%, TEcalc was 1.55%, which was less than TEa set by the NGSP. Both methods strongly correlated with the correlation coefficient (r) 0.9716, p < 0.0001. Conclusion Our study showed HbA1c analysis by PEIT technique is precise, accurate, rapid and convenient and can be employed as an alternative to HPLC technique in countries where cost is a major problem for diagnosis and treatment.

scholarly journals Detection of flumethrin acaricide residues from honey and beeswax using high performance liquid chromatography (HPLC) technique

2020 ◽  
Vol 32 (3) ◽  
pp. 2229-2235 ◽  
Author(s):  
Muhammad Jamal ◽  
Muhmmad Asif Aziz ◽  
Muhammad Naeem ◽  
Zahid Iqbal ◽  
Azeem Khalid ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Hplc Technique

Differential intestinal deconjugation of taurine and glycine bile acid N-acyl amidates in rats

1992 ◽  
Vol 262 (2) ◽  
pp. G351-G358
Author(s):  
R. Zhang ◽  
S. Barnes ◽  
R. B. Diasio
Keyword(s):  
High Performance Liquid Chromatography ◽  
Small Intestine ◽  
Liquid Chromatography ◽  
Bile Acid ◽  
Chenodeoxycholic Acid ◽  
High Performance ◽  
Biliary Fistula ◽  
The Difference ◽  
The Stability ◽  
Rat Bile

Mechanisms responsible for the difference in the relative amounts of taurine- and glycine-conjugated bile acid N-acyl amidates (Tau/Gly ratio) are not fully understood. In the present study, the stability of taurine- and glycine-conjugated bile acid N-acyl amidates during intestinal transit and absorption was examined to investigate the contribution of intestinal deconjugation to the Tau/Gly ratio in rat bile. Radiolabeled chenodeoxycholic acid (CDC) and its N-acyl amidates with glycine (CDC-Gly) or taurine (CDC-Tau) were introduced into the lumen of the upper small intestine in the biliary fistula rats, and radioactive metabolites in bile, blood, urine, and tissues were identified and quantitated by high-performance liquid chromatography. Results indicated that 1) extensive deconjugation of CDC-Gly occurs during intestinal absorption; 2) CDC-Tau is recovered in bile largely intact; and 3) newly synthesized CDC-Tau and CDC-Gly are formed in a ratio of less than 2:1 after administration of [14C]-CDC. In summary, the present study demonstrates that resistance of taurine-conjugated bile acid N-acyl amidates to hydrolysis in the intestine, rather than a difference in synthesis of taurine- and glycine-conjugated N-acyl amidates in liver, may account for the high Tau/Gly ratio in rat bile.

scholarly journals Elimination of Bilirubin Interference in Creatinine Assays by Routine Techniques: Comparisons with a High Performance Liquid Chromatography Method

1994 ◽  
Vol 31 (3) ◽  
pp. 262-266 ◽  
Author(s):  
Sandra Boot ◽  
Nicholas LaRoche ◽  
Edward F Legg
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Reference Method ◽  
Chromatography Method ◽  
The Difference ◽  
Liquid Chromatography Method ◽  
Ideal Method ◽  
Single Slide ◽  
Blank Correction

The aim of this study was to find a method for creatinine analysis unaffected by jaundice by comparing four different methods with high performance liquid chromatography (HPLC) as a reference method. The methods investigated were Kodak DTSC single slide, Boehringer alkaline picrate with and without blank correction and a Boehringer enzymatic method, the last three using the Hitachi 737 analyser. HPLC results were compared with the results of the methods studied. In addition, graphs plotting the difference from the HPLC result against the bilirubin concentration were made; the ideal method would yield a slope and intercept of zero. While all methods showed a positive bias compared with HPLC, the Kodak slide and the Boehringer alkaline picrate with blank correction methods eliminated bilirubin interference and we have changed to the latter method as a consequence of this work.

scholarly journals Comparative Studies on the pH Dependence of DOWof Microcystin-RR and -LR Using LC-MS

2011 ◽  
Vol 11 ◽  
pp. 20-26 ◽  
Author(s):  
Gaodao Liang ◽  
Ping Xie ◽  
Jun Chen ◽  
Ting Yu
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Physicochemical Properties ◽  
Distribution Ratio ◽  
High Performance ◽  
Water Distribution ◽  
Ph Dependence ◽  
Remarkable Difference ◽  
The Difference

Microcystins (MCs) are well known worldwide as hepatotoxins produced by cyanobacteria, but little is known about the physicochemical properties of these compounds. The dependence of the n-octanol/water distribution ratio (DOW) of MC-RR and -LR to pH was measured by high-performance liquid chromatography combined with mass spectrometry (LC-MS). There was a remarkable difference in such relationships between MC-RR and -LR. The log DOWof MC-LR decreased from 1.63 at pH 1.0 to -1.26 at pH 6.5, and stabilized between -1.04 and -1.56 at a pH of 6.5~12.0; log DOWof MC-RR varied between -1.24 and -0.67 at a pH of 1.00~4.00, and stabilized between -1.20 and -1.54 at a pH of 4.00~12.00. The difference of hydrophobicity in acidic condition between MC-RR and -LR is important, not only for the analytical method of both toxins, but perhaps also for understanding the difference of toxicity to animals between the two toxins.

scholarly journals Identification and Quantification of Soil Pesticides in Coastal Lakshmipur District of Bangladesh

2021 ◽  
Vol 46 (2) ◽  
pp. 191-200
Author(s):  
M Amin Uddin ◽  
Nasidulabedin Chowdhury ◽  
Md Abdur Rahman ◽  
Md Harunur Rashid ◽  
M Alamgir Zaman Chowdhury ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Pesticide Residues ◽  
High Performance ◽  
Soil Samples ◽  
Hplc Technique ◽  
Soil Sediments ◽  
Identification And Quantification

This study was carried out to determine the presence and quantity of some selected pesticides from soil sediments collected from some ponds and canals located in the Lakshmipur district of Bangladesh. The high performance liquid chromatography (HPLC) technique was used to determine the concentration of pesticide residues. Some soil samples were found to be contaminated with carbamate (carbofuran and carbaryl) and organophosphorus (diazinon) pesticides. The concentration of carbofuran pesticide ranged from 0.303 μg/kg to 1.851 μg/kg. The highest concentration of carbofuran pesticide was found in SSP6 (1.851 μg/kg) and the lowest concentration was found in SSP9 (0.303 μg/kg). Carbaryl pesticide was found to be present in the sediment of only one pond, the concentration being 1.047 μg/kg. Organophosphorus (diazinon) pesticide was found in soil samples and the concentrations ranged from 0.147 μg/kg to 0.759 μg/kg, which were higher than the EEC-recommended limit of 0.1 μg/kg. Asiat. Soc. Bangladesh, Sci. 46(2): 191-200, December 2020

scholarly journals Capsaicinoids Content in Habanero Pepper (Capsicum chinense Jacq.): Hottest Known Cultivars

HortScience ◽  
2008 ◽  
Vol 43 (5) ◽  
pp. 1344-1349 ◽  
Author(s):  
Adriana Canto-Flick ◽  
Eduardo Balam-Uc ◽  
Jericó Jabìn Bello-Bello ◽  
Carlos Lecona-Guzmán ◽  
Daniela Solís-Marroquín ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Scientific Research ◽  
Intraspecific Diversity ◽  
Capsicum Chinense ◽  
Germplasm Bank ◽  
Habanero Pepper ◽  
Hplc Technique ◽  
Yellow Type

The aim of this study was to determine the pungency level of different accessions of Habanero peppers. The high-performance liquid chromatography (HPLC) technique was used to evaluate the content of total capsaicinoids in the whole fruit, placenta, and pericarp of 18 accessions of Habanero pepper from the germplasm bank of the Capsicum chinense species maintained in the Scientific Research Center of Yucatan [Centro de Investigación Científica de Yucatán (CICY)]. Thirteen of these accessions belonged to the “orange type”, four to the “red type”, and one to the “yellow type”. During the study, the plants were cultivated and maintained under greenhouse conditions and the fruit was harvested only when it was completely ripe on the plant. The results show considerable intraspecific diversity for this characteristic as well as the existence of cultivars of this species that surpass the levels of pungency reported for Habanero peppers under the conditions evaluated.

Quantitative and Chemical Fingerprint Analysis of Desmodium styracifolium by High-Performance Liquid Chromatography Combined with Chemometrics

2019 ◽  
Vol 58 (4) ◽  
pp. 294-302
Author(s):  
Liangyuan Chen ◽  
Xiaomin Tang ◽  
Quan Yang ◽  
Xuanxuan Cheng
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Evaluation Method ◽  
Internal Standard ◽  
Principal Component ◽  
Desmodium Styracifolium ◽  
External Standard Method ◽  
Geographical Regions ◽  
Significant Difference

Abstract In this study, a valid and comprehensive evaluation method for assessing the quality of Desmodium styracifolium (Osb.) Merr has been established, based on analysis of high-performance liquid chromatography fingerprint combined with the similarity analysis (SA), hierarchical cluster analysis (HCA), principal component analysis (PCA), discriminant analysis (DA) and the quantitative analysis multi-components by single marker (QAMS) method. Eleven peaks of the common model were obtained and analyzed using SA, HCA, PCA and DA analysis. These methods indicated a similar conclusion that 31 batches of D. styracifolium samples were categorized into two clusters basically coincident with their geographical regions of origin. Four peaks were identified as schaftoside, isoorientin, isoschaftoside and isovitexin. Schaftoside was selected as the internal standard, and the relative correction factors between schaftoside and the other three flavonoids were calculated using the QAMS method. The accuracy of the QAMS method was verified by comparing with the results calculated by the external standard method. No significant difference between the two methods was found. In conclusion, the established methods were scientifically applied in the quality evaluation of D. styracifolium.

scholarly journals Hb A1c Separation by High Performance Liquid Chromatography in Hemoglobinopathies

Scientifica ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-4 ◽  
Author(s):  
Vani Chandrashekar
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Rank Correlation ◽  
Beta Thalassemia ◽  
Compound Heterozygous ◽  
Hb A1c ◽  
Spearman’S Rank Correlation ◽  
Significant Difference ◽  
The Difference

Hb A1c measurement is subject to interference by hemoglobin traits and this is dependent on the method used for determination. In this paper we studied the difference between Hb A1c measured by HPLC in hemoglobin traits and normal chromatograms. We also studied the correlation of Hb A1c with age. Hemoglobin analysis was carried out by high performance liquid chromatography. Spearman’s rank correlation was used to study correlation between A1c levels and age. Mann-WhitneyUtest was used to study the difference in Hb A1c between patients with normal hemoglobin and hemoglobin traits. A total of 431 patients were studied. There was positive correlation with age in patients with normal chromatograms only. No correlation was seen in Hb E trait or beta thalassemia trait. No significant difference in Hb A1c of patients with normal chromatograms and patients with hemoglobin traits was seen. There is no interference by abnormal hemoglobin in the detection of A1c by high performance liquid chromatography. This method cannot be used for detection of A1c in compound heterozygous and homozygous disorders.

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