miR221 regulates cell migration by targeting annexin a1 expression in human mesothelial MeT-5A cells neoplastic-like transformed by multi-walled carbon nanotube

Abstract Background Multi-walled carbon nanotube (MWCNT) is one of the most widely used manufactured nanomaterials, however, its potential harmful effect on human health is of great concern. Previously we have shown the acute and chronic exposure to MWCNT induced different responses in human mesothelial MeT-5A cells. In the current study, MeT-5A cells were continuously subjected to MWCNT exposure at 10 μg/cm2 for 48 h per passage, up to a whole year, to further clarify the carcinogesis and its potential mechanisms of MWCNT. Results After one-year MWCNT treatment, MeT-5A cells exhibited neoplastic-like properties, including morphological changes, anchorage-independent growth, increased cell proliferation and cell migration. Further examination revealed the expression of microRNA 221 (miR221) was gradually decreased, while the annexin a1 expression was increased at both the mRNA and protein level during the exposure. Bioinformatic analysis indicated that annexin a1 is a target for miR221 regulation, and it was confirmed by transfecting cells with miR221 mimics, which resulted in the downregulation of annexin a1. Detailed analyses demonstrated miR221 was involved in the regulation of cell migration, e.g., downregulation of miR221 or overexpression of ANNEXIN A1, contributed to the increased cell migration. In contrast, overexpression of miR221 or downregulation of ANNEXIN A1 slowed cell migration. Conclusions Taken together, these results point to a neoplastic-transforming property of MWCNT, and the miR221-annexin a1 axis is involved in the regulation of cell migration in the transformed cells.

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Physicochemical predictors of Multi-Walled Carbon Nanotube-induced pulmonary histopathology and toxicity one year after pulmonary deposition of 11 different Multi-Walled Carbon Nanotubes in mice

Basic & Clinical Pharmacology & Toxicology ◽

10.1111/bcpt.13119 ◽

2018 ◽

Vol 124 (2) ◽

pp. 211-227 ◽

Cited By ~ 23

Author(s):

Kristina B. Knudsen ◽

Trine Berthing ◽

Petra Jackson ◽

Sarah S. Poulsen ◽

Alicja Mortensen ◽

...

Keyword(s):

Carbon Nanotubes ◽

Carbon Nanotube ◽

Pulmonary Deposition ◽

Multi Walled Carbon Nanotube ◽

Multi Walled Carbon Nanotubes ◽

One Year ◽

Walled Carbon Nanotubes

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Modulation of electronic conductivity and bandgap of electrochemically polymerised polycarbazole films using montmorillonite, multi‐walled carbon nanotube and reduced graphene oxide as nanofillers

Micro & Nano Letters ◽

10.1049/mnl.2018.0139 ◽

2018 ◽

Vol 13 (9) ◽

pp. 1335-1338

Author(s):

Aditi Srivastava ◽

P. Chakrabarti

Keyword(s):

Graphene Oxide ◽

Carbon Nanotube ◽

Reduced Graphene Oxide ◽

Electronic Conductivity ◽

Multi Walled Carbon Nanotube ◽

Reduced Graphene

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Surface Morphology Changes of Lithium/Sulfur Battery using Multi-walled carbon nanotube added Sulfur Electrode during Cyclings

Korean Journal of Metals and Materials ◽

10.3365/kjmm.2011.49.2.174 ◽

2011 ◽

Vol 49 (2) ◽

Keyword(s):

Carbon Nanotube ◽

Surface Morphology ◽

Lithium Sulfur Battery ◽

Multi Walled Carbon Nanotube ◽

Sulfur Battery ◽

Sulfur Electrode ◽

Morphology Changes ◽

Lithium Sulfur

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Electrical characteristics of multi-walled carbon nanotube-polyethylene composites by catalyst and gas control

Journal of Ceramic Processing Research ◽

10.36410/jcpr.2019.20.5.464 ◽

2019 ◽

Vol 20 (5) ◽

pp. 464-469

Author(s):

Suyoung Park ◽

Changhyun Jin ◽

Sun-Woo Choi

Keyword(s):

Carbon Nanotube ◽

Electrical Characteristics ◽

Multi Walled Carbon Nanotube ◽

Gas Control ◽

Polyethylene Composites

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Capacitance imaging for the discrimination of lipid region in atherosclerotic plaque ex vivo using polypyrrole-coated multi-walled carbon nanotube multi-electrode array

Current Applied Physics ◽

10.1016/j.cap.2019.08.006 ◽

2019 ◽

Vol 19 (11) ◽

pp. 1238-1244

Author(s):

Hyung Joon Kim

Keyword(s):

Carbon Nanotube ◽

Atherosclerotic Plaque ◽

Ex Vivo ◽

Electrode Array ◽

Multi Walled Carbon Nanotube ◽

Multi Electrode Array

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Photoluminescent Properties of Hydroxyapatite and Hydroxyapatite/Multi-Walled Carbon Nanotube Composites

Crystals ◽

10.3390/cryst11070832 ◽

2021 ◽

Vol 11 (7) ◽

pp. 832

Author(s):

Edna X. Figueroa-Rosales ◽

Javier Martínez-Juárez ◽

Esmeralda García-Díaz ◽

Daniel Hernández-Cruz ◽

Sergio A. Sabinas-Hernández ◽

...

Keyword(s):

Carbon Nanotube ◽

Hexagonal Phase ◽

Precipitation Method ◽

X Ray Diffraction ◽

X Ray ◽

Multi Walled Carbon Nanotube ◽

Nanotube Composites ◽

Carbon Nanotube Composites ◽

Co Precipitation ◽

Functionalized Mwcnts

Hydroxyapatite (HAp) and hydroxyapatite/multi-walled carbon nanotube (MWCNT) composites were obtained by the co-precipitation method, followed by ultrasound-assisted and microwave radiation and thermal treatment at 250 °C. X-ray diffraction (XRD) confirmed the presence of a hexagonal phase in all the samples, while Fourier-transform infrared (FTIR) spectroscopy elucidated the interaction between HAp and MWCNTs. The photoluminescent technique revealed that HAp and the composite with non-functionalized MWCNTs present a blue luminescence, while the composite with functionalized MWCNTs, under UV-vis radiation shows an intense white emission. These findings allowed presentation of a proposal for the use of HAp and HAp with functionalized MWCNTs as potential materials for optoelectronic and medical applications.

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Acute and chronic exposure to air pollution in relation with incidence, prevalence, severity and mortality of COVID-19: a rapid systematic review

Environmental Health ◽

10.1186/s12940-021-00714-1 ◽

2021 ◽

Vol 20 (1) ◽

Author(s):

Patrick D. M. C. Katoto ◽

Amanda S. Brand ◽

Buket Bakan ◽

Paul Musa Obadia ◽

Carsi Kuhangana ◽

...

Keyword(s):

Air Pollution ◽

Chronic Exposure ◽

Ambient Air ◽

Short Term ◽

Incidence And Mortality ◽

Acute And Chronic Exposure ◽

Anthropogenic Pollutant ◽

Incident Cases ◽

Incidence Prevalence

Abstract Background Air pollution is one of the world’s leading mortality risk factors contributing to seven million deaths annually. COVID-19 pandemic has claimed about one million deaths in less than a year. However, it is unclear whether exposure to acute and chronic air pollution influences the COVID-19 epidemiologic curve. Methods We searched for relevant studies listed in six electronic databases between December 2019 and September 2020. We applied no language or publication status limits. Studies presented as original articles, studies that assessed risk, incidence, prevalence, or lethality of COVID-19 in relation with exposure to either short-term or long-term exposure to ambient air pollution were included. All patients regardless of age, sex and location diagnosed as having COVID-19 of any severity were taken into consideration. We synthesised results using harvest plots based on effect direction. Results Included studies were cross-sectional (n = 10), retrospective cohorts (n = 9), ecological (n = 6 of which two were time-series) and hypothesis (n = 1). Of these studies, 52 and 48% assessed the effect of short-term and long-term pollutant exposure, respectively and one evaluated both. Pollutants mostly studied were PM2.5 (64%), NO2 (50%), PM10 (43%) and O3 (29%) for acute effects and PM2.5 (85%), NO2 (39%) and O3 (23%) then PM10 (15%) for chronic effects. Most assessed COVID-19 outcomes were incidence and mortality rate. Acutely, pollutants independently associated with COVID-19 incidence and mortality were first PM2.5 then PM10, NO2 and O3 (only for incident cases). Chronically, similar relationships were found for PM2.5 and NO2. High overall risk of bias judgments (86 and 39% in short-term and long-term exposure studies, respectively) was predominantly due to a failure to adjust aggregated data for important confounders, and to a lesser extent because of a lack of comparative analysis. Conclusion The body of evidence indicates that both acute and chronic exposure to air pollution can affect COVID-19 epidemiology. The evidence is unclear for acute exposure due to a higher level of bias in existing studies as compared to moderate evidence with chronic exposure. Public health interventions that help minimize anthropogenic pollutant source and socio-economic injustice/disparities may reduce the planetary threat posed by both COVID-19 and air pollution pandemics.

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