scholarly journals Complicated cutaneous leishmaniasis caused by an imported case of Leishmania tropica in Japan: a case report

2021 ◽  
Vol 49 (1) ◽  
Author(s):  
Hiroyuki Kitano ◽  
Chizu Sanjoba ◽  
Yasuyuki Goto ◽  
Kazumasa Iwamoto ◽  
Hiroki Kitagawa ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Skin Lesions ◽  
Leishmania Tropica ◽  
Case Presentation ◽  
Imported Case ◽  
Imported Cases ◽  
History Of ◽  
Hematoxylin And Eosin ◽  
Polymerase Chain ◽  
Positive Results

Abstract Background Leishmaniasis is not endemic in Japan, and imported cases are rare. However, there are increasing concerns regarding imported cases of cutaneous leishmaniasis from endemic countries to Japan. This report describes a case of imported cutaneous leishmaniasis that was diagnosed and treated in Japan. Case presentation A 53-year-old Pakistani man presented with skin lesions on both malleoli of his right ankle and the dorsum of the left foot. The skin lesions manifested as erythematous nodules surrounding an ulcer in the center of the lesion. The lesions of the malleoli of his right ankle each measured 3 × 3 cm, and the lesion on the top of his left foot measured 5 × 4 cm. He had been living and working in Japan but had a history of a visit to Pakistan for about 2 months in 2018. The skin lesions were biopsied. Giemsa and hematoxylin and eosin staining of biopsy samples showed amastigotes of Leishmania in macrophages, and the presence of Leishmania was confirmed by skin tissue culture. Polymerase chain reaction using biopsy specimens identified Leishmania parasites, and DNA sequence analysis revealed that the species was Leishmania tropica. The patient was treated with intravenous liposomal amphotericin B for 6 days. The erythema disappeared, and the erythematous nodules resolved within 3 weeks. Conclusion This is the first report of imported cutaneous leishmaniasis caused by L. tropica from Pakistan, and it is interesting that all three testing modalities showed positive results in this case.

scholarly journals Hoarseness of Voice as a Rare Presentation of Tuberculosis: A Case Report Study

2019 ◽  
Vol 7 (19) ◽  
pp. 3262-3264
Author(s):  
Taher Felemban ◽  
Abdullah Ashi ◽  
Abdullah Sindi ◽  
Mohannad Rajab ◽  
Zuhair Al Jehani
Keyword(s):  
Laryngeal Carcinoma ◽  
Incidental Finding ◽  
Chain Reaction ◽  
Rare Presentation ◽  
Case Presentation ◽  
X Ray ◽  
History Of ◽  
Chest X Ray ◽  
Polymerase Chain ◽  
Laryngeal Tuberculosis

BACKGROUND: Having hoarseness of voice as the first clinical manifestation of tuberculosis is rare. This atypical presentation causes some confusion since other more common conditions, such as laryngeal carcinoma, present similarly and might require more invasive tests to confirm the diagnosis. CASE PRESENTATION: A 38-year-old male presented to the otorhinolaryngology clinic with a four-month history of change in voice. Laryngoscopy demonstrated a right glottic mass, raising suspicion of laryngeal cancer. The computed tomography showed a mass and incidental finding of opacities in lung apices. Chest x-ray demonstrated findings suggestive of tuberculosis. Polymerase chain reaction and culture of sputum samples confirmed the diagnosis and the patient was started on anti-tuberculosis treatment. CONCLUSION: Despite accounting for only 1% of pulmonary tuberculosis cases and having a similar presentation to laryngeal carcinoma, we recommend considering laryngeal tuberculosis when evaluating hoarseness of voice in endemic areas.

scholarly journals Leishmania major cause of a cutaneous leishmaniasis in a car driver from east Azerbaijan province: A case report

2021 ◽  
Author(s):  
Mahdi parsaei ◽  
Shalaleh Ganji ◽  
Majid Khanmohammadi
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Leishmania Major ◽  
Hematological Parameters ◽  
Electrophoretic Pattern ◽  
Positive Control ◽  
Case Presentation ◽  
Pcr Product ◽  
Sequencing Method ◽  
History Of ◽  
East Azerbaijan

Background: Leishmaniasis is a zoonotic disease and its the known as a health problem in all of the world. Case presentation: The patient, a 47-year-old man car driver from city of Bostan abad, with a history of traveling to cutaneous leishmaniasis endemic provinces last year, was referred to city health center with a 17 mm, no discharge and pus with necrotic appearance skin lesion. Biochemical, hematological parameters and urine culture and analysis were normal. Three smears was prepared with sterile vaccinostyle from the margins of wound and transferred to specific medium in sterile conditions. After staining the prepared smears from the wound, amastigotes with large nucleus and small kinetoplast in macrophages and active promastigotes in the liquid phase of medium were observed. Polymerase chain reaction (PCR) was performed using ITS-1 by specific primers. In the PCR product electrophoresis were compared with the marker bands and 350 bp band weight determined. With electrophoretic pattern and comparison with positive control band, isolates parasites belonged to Leishmania genus. In order to definitively diagnose the parasite species, sequencing method was used. The results showed 99% homology with Leishmania major. Finally, the patient with a diagnosis of rural cutaneous leishmaniasis was induction with Glucantime drug. Conclusion: Diagnosis of suspected cases with cutaneous leishmaniasis is the major importance. If an infected patient is diagnosed in the early stages of the disease, can be prevented the extent of the scar after recovery, as well as the possible complications of rural infection.

scholarly journals Cutaneous leishmaniasis in north Lebanon: re-emergence of an important neglected tropical disease

2019 ◽  
Vol 113 (8) ◽  
pp. 471-476 ◽  
Author(s):  
Dima El Safadi ◽  
Sabah Merhabi ◽  
Rayane Rafei ◽  
Hassan Mallat ◽  
Monzer Hamze ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Leishmania Major ◽  
Medical Center ◽  
Tropical Disease ◽  
Neglected Tropical Disease ◽  
Show Evidence ◽  
History Of ◽  
Displaced People ◽  
Mass Influx ◽  
Polymerase Chain

Abstract Background Cutaneous leishmaniasis (CL) is the most prevalent neglected tropical disease among externally displaced people in the Middle East. In recent years, the Lebanese population has increased >30%, mainly due to a mass influx of Syrian migrants, thousands of them carrying CL, among other infectious diseases. Here we revisit the current CL prevalence among refugees in northern Lebanon. Methods This cohort study was conducted at the Al Bashaer Medical Center in north Lebanon between January and June 2017. A total of 48 randomly selected suspected CL patients were clinically diagnosed by dermatologists and samples were obtained for microscopic examination and molecular identification by polymerase chain reaction restriction fragment length polymorphism. The treatment response to antimonials was assessed each week and was followed for up 6 months. Results Leishmania tropica was the predominant species (91.7%) followed by Leishmania major (8.3%). Confirmed cases were treated with one to two courses of antimonials and healing was usually achieved after receiving a second course of treatment. Importantly, we show evidence of possible local CL transmission by indigenous sandflies in three separate patients who had no history of recent travel to Syria. Conclusions This highlights the urgent necessity to implement preventive disease strategies to avoid further dispersion of L. tropica CL in north Lebanon.

scholarly journals Sudden neck swelling with rash as late manifestation of COVID-19: a case report

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Caterina Giannitto ◽  
Cristiana Bonifacio ◽  
Susanna Esposito ◽  
Angela Ammirabile ◽  
Giuseppe Mercante ◽  
...  
Keyword(s):  
Subcutaneous Tissue ◽  
Esophageal Wall ◽  
Nasopharyngeal Swab ◽  
Neck Swelling ◽  
Case Presentation ◽  
Patient Reported ◽  
Good Clinical Condition ◽  
History Of ◽  
Collateral Findings ◽  
Polymerase Chain

Abstract Background Although there are reports of otolaryngological symptoms and manifestations of CoronaVirus Disease 19 (COVID-19), there have been no documented cases of sudden neck swelling with rash in patients with Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection described in literature. Case presentation We report a case of a sudden neck swelling and rash likely due to late SARS-CoV-2 in a 64-year-old woman. The patient reported COVID-19 symptoms over the previous three weeks. Computed Tomography (CT) revealed a diffuse soft-tissue swelling and edema of subcutaneous tissue, hypodermis, and muscular and deep fascial planes. All the differential diagnoses were ruled out. Both the anamnestic history of the patient’s husband who had died of COVID-19 with and the collateral findings of pneumonia and esophageal wall edema suggested the association with COVID-19. This was confirmed by nasopharyngeal swab polymerase chain reaction. The patient was treated with lopinavir/ritonavir, hydroxychloroquine and piperacillin/tazobactam for 7 days. The neck swelling resolved in less than 24 h, while the erythema was still present up to two days later. The patient was discharged after seven days in good clinical condition and with a negative swab. Conclusion Sudden neck swelling with rash may be a coincidental presentation, but, in the pandemic context, it is most likely a direct or indirect complication of COVID-19.

scholarly journals Simultaneous development of COVID-19 pneumonia and pulmonary metastasis in a known case of chondrosarcoma: a case report

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Afshin Rakhsha ◽  
Zahra Mahboubi-Fooladi ◽  
Anya Jafari
Keyword(s):  
Pulmonary Metastasis ◽  
Pulmonary Nodules ◽  
Simultaneous Presentation ◽  
Case Presentation ◽  
History Of ◽  
Polymerase Chain ◽  
Lung Ct ◽  
Chest Ct Scan ◽  
Interlobular Septal Thickening

Abstract Background The outbreak of coronavirus disease 2019 (COVID-19) started in December 2020, and is a global problem now. There are several sets of established data regarding computed tomography (CT) findings in COVID-19 pneumonia with many differential diagnoses. During the early days of the pandemic, there was little data regarding lung CT features of COVID-19 in a cancer patient. In this paper, we described a rare case of simultaneous presentation of COVID-19 with pulmonary metastasis. Case presentation A Persian patient with a history of chondrosarcoma presented to our clinic during the COVID-19 pandemic with a new-onset cough. He had experienced no recurrence during previous follow-up visits. Chest CT scan revealed numerous bilateral small peripheral and perilymphatic pulmonary nodules, unilateral ground-glass patch, and nodular interlobular septal thickening. Biopsy of the pulmonary nodules established pulmonary metastasis of chondrosarcoma origin, and pharyngeal reverse transcription polymerase chain reaction (RT-PCR) was positive for COVID-19. Conclusion Pulmonary metastasis should be considered as a differential diagnosis of COVID-19 features in cancer patients in the pandemic era.

scholarly journals Seroprevalence and Molecular Detection of Influenza A Virus (H1N1) in Sulaimani Governorate-Iraq

2022 ◽  
Vol 6 (1) ◽  
pp. 1-6
Author(s):  
Kaziwa Ahmad Kaka alla ◽  
Salih Ahmed Hama
Keyword(s):  
Employment Status ◽  
Influenza A ◽  
H1n1 Virus ◽  
Previous History ◽  
Reverse Transcription Pcr ◽  
Influenza A H1n1 ◽  
History Of ◽  
Polymerase Chain ◽  
Pcr Techniques ◽  
Positive Results

Influenza A (H1N1) virus is now rapidly scattering across the world. Early detection is one of the most effective measures to stop the further spread of the virus. The current study was aimed to detect influenza A (H1N1) serologically and by polymerase chain reaction (PCR) techniques. From September 2020 to June 2021, three hundred nasopharyngeal swabs and blood samples were collected from Hiwa and Shahid Tahir Hospitals in Sulaimani city. Obtained results revealed that 23.3% of the tested patients were seropositive anti-IgG for Influenza A, while 13.3% showed anti-IgM seropositive results although 10% of the tested cases were with both anti-IgG and anti-IgM seropositive results. Gender, residency, and flu symptoms showed no significant relations with seropositive results (p<0.05) whereas valuable relations were found between seropositive observations and smoking, the previous history of chronic diseases as well as employment status (p<0.05). It was concluded that hematologic investigations (CBC) were not dependable if H1N1 diagnosis and detection. Only 1% of the tested samples showed positive results for influenza A (H1N1) RNA using reverse transcription-PCR.

scholarly journals MICROSCOPIC OBSERVATION OF VARIOUS ORGANS OF PIGEONS WITH SYMPTOMP OF TORTICOLLIS AND IDENTIFICATION OF NEWCASTLE DISEASE VIRUS BASED ON AGAR GEL PRECIPITATION TEST

2020 ◽  
Vol 21 (4) ◽  
pp. 519-529
Author(s):  
Risang Aji Dewandaru ◽  
Ferdinand Prayogo Cahyo Santoso ◽  
Sugiyono Sugiyono ◽  
Michael Haryadi Wibowo
Keyword(s):  
Histopathological Examination ◽  
Allantoic Fluid ◽  
White Pulp ◽  
Agar Gel ◽  
Pulmonary Tissue ◽  
Serological Identification ◽  
History Of ◽  
Hematoxylin And Eosin ◽  
Positive Results ◽  
Precipitation Test

This study aimed to determine the microscopic conditions of organs of pigeons that suffered from torticollis and identify the cause of disease in torticollis pigeons. Three pigeons showed torticollis symptoms were obtained as sample and marked as pigeon A, B and C, respectively. Isolation of pathogen with inoculation into embryonated pigeon eggs obtained from parents with no history of vaccination and not indicated ND. Identification of ND with agar gel precipitation (AGP) test. Observation of microscopical changes with histopathologic preparation using hematoxylin and eosin staining. Histopathological examination showed that pigeons was done severe neuritis vagus, trakheitis, pneumonia, air sacculitis, hepatitis, pankreatitis, nefritis, jejunoileitis, ileocolitis and orchitis. Perivascular cuffing found in brain. Degenerative changes found in the hepar and ren. Cardiac severe necrotic lesion, and depletion in white pulp area of spleen. Proventricular tissue showed flattening of mucosal epithelium, congestion lesions found in pulmonary tissue. The results of slow hemagglutination test of pigeon egg allantoic fluid, which tested for hemagglutination (HA) showed positive HA result with titers varying between 2, 32, and 64. Serological identification carried out with the AGP test on all culture samples against ND antiserum showing positive results of ND virus. Based on these dara, it can be concluded that the pigeons with symptoms of torticollis is caused by ND virus.

scholarly journals Changes in the microbiological diagnosis and epidemiology of cutaneous leishmaniasis in real-time PCR era: A six-year experience in a referral center in Barcelona

2021 ◽  
Vol 15 (11) ◽  
pp. e0009884
Author(s):  
Aroa Silgado ◽  
Mayuli Armas ◽  
Adrián Sánchez-Montalvá ◽  
Lidia Goterris ◽  
Maria Ubals ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Real Time ◽  
Real Time Pcr ◽  
Skin Lesions ◽  
Tertiary Hospital ◽  
Molecular Techniques ◽  
Cutaneous Lesions ◽  
Imported Cases ◽  
Epidemiological Characteristics

Background Leishmaniasis is a neglected disease caused by different species of the protozoa Leishmania spp. Cutaneous lesions are the most common clinical manifestation. This disease is prevalent in tropical and subtropical areas, including the Mediterranean basin. In Spain, Leishmania (L.) infantum is the only endemic species, but imported cases are often diagnosed. Different classical parasitological methods can be performed for cutaneous leishmaniasis (CL) diagnosis; but currently molecular techniques serve as a relevant tool for the detection and characterization of Leishmania parasites. We aimed to evaluate clinical and epidemiological characteristics of CL diagnosed patients by real-time PCR in a tertiary hospital over a six-year period. Methodology/Principal findings Clinical, epidemiological and microbiological data were retrospectively collected and analyzed. In our study, CL was confirmed in 59 (31.4%) out of 188 patients by real-time PCR, showing an increase over recent years: 11 cases of CL between 2014 and 2016 and 48 between 2017 and 2019. Real-time PCR was performed on skin swabs and/or biopsies samples, with a positivity of 38.5% and 26.5%, respectively. Results were 100% concordant when biopsy and skin swab were performed simultaneously. L. (L.) infantum was the most frequent species detected (50%), followed by L. (L.) major (45%) and Viannia subgenus (5%), which were detected only in imported cases. L. (L.) major was almost entirely detected in travelers/migrants from Morocco. Multiple and atypical skin lesions were more common in imported cases than in autochthonous cases (44.4% vs. 21.8%). Conclusions/Significance An increase in both autochthonous and imported CL cases has been observed in past years in our hospital. Molecular techniques assist in improving CL diagnosis and characterization of the Leishmania species, mainly in imported cases.

First Autochthonous Case of Emerged Zoonotic Cutaneous Leishmaniasis in Mazandaran Province, Northern Iran: kDNA-PCR Evidence Base

2020 ◽  
Vol 20 ◽  
Author(s):  
Fatemeh Niksolat ◽  
Rabeeh Tabaripour ◽  
Lotfollah Davoodi ◽  
Mahdi Fakhar
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Leishmania Major ◽  
Skin Lesions ◽  
Needle Aspiration ◽  
Evidence Base ◽  
Mazandaran Province ◽  
Northern Iran ◽  
Zoonotic Cutaneous Leishmaniasis ◽  
Smear Preparation ◽  
History Of

: In the last decade, several cases of cutaneous leishmaniasis (CL) have been recorded by the provincial health center of Mazandaran Province, northern Iran. However, there is no documented report of autochthonous CL in the province yet. The patient, a 59-year-old female, known case of diabetes mellitus, lived in rural area of Kiasar district (in eastern part of the Mazandaran) with 5 skin lesions suspected of having vasculitis, without history of traveling to the endemic areas of CL, was admitted to Imam Khomeini teaching hospital, Sari. To rule out CL, fine needle aspiration (FNA) procedure was used aseptically for the patient. Laboratory investigations showed Leishman bodies (amastigotes) by direct smear preparation and species identification confirmed Leishmania major (L. major) by polymerase chain reaction (PCR) molecular method. Accordingly, this is the first documented report of autochthonous CL in Mazandaran Province. Overall, due to the abundance of probable rodent reservoir hosts and confirmation of infection of sandflies (Phlebotomus papatasi) with L. major in the Kiasar district, where our patient was living there, it seems that CL emerged in the eastern part of the province. Therefore, further studies on rodents and sandflies fauna in terms of emerging leishmanial infection in the area is recommended.

scholarly journals Imported cutaneous leishmaniasis: molecular investigation unveils Leishmania major in Bangladesh

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Md Anik Ashfaq Khan ◽  
Rajashree Chowdhury ◽  
Rupen Nath ◽  
Sören Hansen ◽  
Progga Nath ◽  
...  
Keyword(s):  
Skin Biopsy ◽  
Cutaneous Leishmaniasis ◽  
Leishmania Donovani ◽  
Leishmania Major ◽  
Migrant Worker ◽  
Human Mobility ◽  
Skin Lesions ◽  
Careful Consideration ◽  
Molecular Tests ◽  
Imported Cases

Abstract Background The main clinical forms of leishmaniasis in Bangladesh are visceral leishmaniasis and post-kala-azar dermal leishmaniasis, which are caused by Leishmania donovani. Imported cutaneous leishmaniasis (CL) is emerging globally due mainly to increased human mobility. In recent years, several imported CL cases have also been reported in Bangladesh. Sporadic atypical cases of CL can be challenging for diagnosis and clinical management, while occurrence of infection on a frequent basis can be alarming. We report of a case of a Bangladeshi temporary-migrant worker who, upon return, presented development of skin lesions that are characteristic of CL. Methods A serum sample was collected and tested with an rK39 immunochromatographic test. Nucleic acid from skin biopsy derived culture sample was extracted and screened with a real-time PCR assay which targets the conserved REPL repeat region of L. donovani complex. The internal transcribed spacer 2 region of the ribosomal RNA gene cluster was amplified and sequenced. Results The suspect had a history of travel in both CL and VL endemic areas and had a positive rK39 test result. Based on clinical presentation, travel history and demonstration of the parasite in the skin biopsy, CL was diagnosed and the patient underwent a combination therapy with Miltefosine and liposomal amphotericin B. While typical endemic species were not detected, we identified Leishmania major, a species that, to our knowledge, has never been reported in Bangladesh. Conclusions Proper monitoring and reporting of imported cases should be given careful consideration for both clinical and epidemiological reasons. Molecular tests should be performed in diagnosis to avoid dilemma, and identification of causative species should be prioritized.

Sign in / Sign up

Export Citation Format

Share Document