scholarly journals Mitochondrial DNA haplogroup study: residents of Sulaymaniyah city in the Iraqi Kurdistan Region may be genetically closer to European lineage

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Han Nihad Mohammed Fadhl ◽  
Shad Arif Mohammed ◽  
Farhad M. Abdulkarim
Keyword(s):  
Mitochondrial Dna ◽  
Single Nucleotide Polymorphisms ◽  
Dna Sequence ◽  
Nucleotide Polymorphisms ◽  
Kurdistan Region ◽  
Maternal Lineage ◽  
Single Nucleotide ◽  
Iraqi Kurdistan ◽  
Fertile Crescent ◽  
Coding Control

Abstract Background Being the native inhabitants of the Neolithic Fertile Crescent, Kurds were included in several maternal lineage studies concerning the Eurasian population. However, no study was performed on the Kurdish population of Sulaymaniyah city (latitude 33.314690 and longitude 44.376759). This study was carried out on a sample of Sorani Kurds living in Sulaymaniyah for the identification of population-related single nucleotide polymorphisms (SNPs) and modes of maternal lineage. Results In this study, 36 randomly selected healthy unrelated Kurdish subjects were enrolled. Whole mitochondrial DNA sequencing was performed. HaploGrep 2.0 and neutrality test (Tajima’s D) were employed for haplogroup identification and historical demography determination. When the outcomes were compared with previous studies in Kurds and the neighbouring nations, the identified haplogroups in the sample of study were members of the Western Eurasian haplogroups with a predominance of haplogroup H. Conclusions The whole mitochondrial DNA sequence is superior to the traditional analysis of the non-coding (control) region. Our study indicates a stronger relation of the studied group to the European lineage than to their neighbouring nations.

P–517 The relationship between mitochondrial gene CYB (MT-CYB) single nucleotide polymorphisms and male infertility

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
M Jawish ◽  
F W Dahadhah ◽  
M Ei. Hammadeh ◽  
H Amor
Keyword(s):  
Mitochondrial Dna ◽  
Single Nucleotide Polymorphisms ◽  
Male Infertility ◽  
Male Fertility ◽  
Mitochondrial Gene ◽  
Nucleotide Polymorphisms ◽  
Control Groups ◽  
Mt Dna ◽  
Single Nucleotide ◽  
Frequency Test

Abstract Study question Do single nucleotide polymorphisms of the mitochondrial gene CYB (MT-CYB) affect male fertility? Summary answer there was a significant association between male fertility and rs527236194, rs28357373, and rs41504845 single nucleotide polymorphisms of the mitochondrial gene CYB (MT-CYB). What is known already Male infertility can be occurred as a result of various factors. However, genetic factors are detected in 15% of male infertility cases and can be classified into two groups: chromosomal abnormalities and single gene mutations. Sperm mitochondrial DNA alterations may have serious effects on spermatogenesis, sperm motility and the ability of sperm to fertilize the oocyte. Mutations of the MT-CYB gene might lead to various disorders and deficiencies specially in complex III which might interrupt in the ATP production process. Study design, size, duration: 111 semen samples were included in this prospectively designed study which carried out between 2017 and August 2019. Participants/materials, setting, methods: This study carried out at the Department of Obstetrics and Gynecology at Saarland University, Germany. Samples were divided into 67 subfertile “cases” and 44 fertile “control” groups. After preparation of semen samples by density gradient centrifugation, nuclear and mitochondrial DNA (MT-DNA) was extracted using QIAamp DNA Mini Kit from QIAGEN. Thereafter, the MT-DNA was amplified using REPLI-g Mitochondrial DNA Kit from QIAGEN, followed by PCR and Sanger sequencing steps. Main results and the role of chance A total of 13 single nucleotide polymorphisms (SNPs) in the MT-CYB gene in each of the case and control groups were detected. Eight SNPs were non-synonymous variants including: rs2853508, rs28357685, rs41518645, rs2853507, rs28357376, rs35070048, rs2853506, and rs28660155 and five SNPs were synonymous variants: rs527236194, rs28357373, rs28357369, rs41504845, and rs2854124.Among these SNPs, three of them showed a significant difference in the genotype’s frequency test between sub-fertile and fertile groups: rs527236194 (T15784C; P = 0.0005), rs28357373 (T15629C; P = 0.0439), and rs41504845 (C15833T; P = 0.0038). For the allele’s frequency test, two SNPs were significant: rs527236194 (T15784C; P = 0.0014) and rs41504845 (C15833T; P = 0.0147). Limitations, reasons for caution The study population size Wider implications of the findings: A larger prospective study will be required to confirm the associations between these mitochondrial gene polymorphisms in MT-CYB (rs527236194, rs28357373, rs41504845) and male infertility and to clarify the definite effect of the mitochondrial genetic variations on male infertility. Trial registration number Not applicable

Sign in / Sign up

Export Citation Format

Share Document