scholarly journals Management of root-knot nematode Meloidogyne incognita of eggplant using some growth-promoting rhizobacteria and chitosan under greenhouse conditions

2020 ◽  
Vol 30 (1) ◽  
Author(s):  
Usama Samy Elkelany ◽  
Nehal Samy El-Mougy ◽  
Mokhtar Mohamed Abdel-Kader
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Meloidogyne Incognita ◽  
Growth Parameters ◽  
Azotobacter Chroococcum ◽  
Economic Losses ◽  
Egg Mass ◽  
Root Knot Nematode ◽  
Wide Range ◽  
Growth Promoting

Abstract Background Eggplant (Solanum melongena) is one of the most popular vegetable crops in Egypt. It is affected by a wide range of pests; prominent among them is root-knot nematode (RKN) Meloidogyne spp. which constitutes a major group of plant-parasitic nematodes causing great economic losses worldwide especially in Egypt. Main body The present investigation was performed under greenhouse conditions to evaluate the potentials of 3 growth-promoting bacteria, Azospirilum brasilense, Azotobacter chroococcum, and Bacillus subtilis, as well as chitosan for the control of the nematode Meloidogyne incognita causes root-knot disease of eggplant cv. Baladi. All treatments reduced (p ≤ 0.05) the nematode population in soil and roots as well as enhanced the plant growth parameters of eggplant remarkably than the control. The applied treatments varied in their efficacy against the plant nematode infection in correspondence to the time of application. The recorded results demonstrated that maximum reduction in J2 in soil, egg mass/root, and eggs/egg masses were obtained by treating the soil with Bacillus subtilis followed by Azospirilum brasilense and Azotobacter chroococcum. Also, chitosan resulted in high reduction in root galls compared to control treatment. Conclusion The use of plant growth-promoting rhizobacteria, Azospirilum brasilense, Azotobacter chroococcum, Bacillus subtilis, and chitosan achieved efficient control to Meloidogyne incognita and consequently increase eggplant growth parameters under greenhouse conditions. The present results suggested introducing such rhizobacteria in integrated nematode management program.

scholarly journals Efficacy of Some Bacterial Biocontrol Agents as Seed Treatment against Root Knot Nematode, Meloidogyne incognita on Tomato

2020 ◽  
Vol 9 (9) ◽  
pp. 1043-1046
Author(s):  
Karter Nyodu ◽  
Debanand Das
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Root System ◽  
Bacillus Megaterium ◽  
Meloidogyne Incognita ◽  
Seed Treatment ◽  
Bacillus Pumilus ◽  
Growth Parameters ◽  
Biocontrol Agents ◽  
Root Knot Nematode

Pot experiment was conducted to evaluate the efficacy of two formulations (talc formulation and vermi formulation) of four bacterial nematode biocontrol agents viz., Bacillus subtilis, Bacillus pumilus, Bacillus megaterium and Pseudomonus flourescens as seed treatment against root knot nematode, Meloidogyne incognita on tomato. All the bioagents were tries at 10 and 20g / kg of seed. The experiment was terminated at 60 days after sowing the seed. The results revealed that all the treatments significantly increased the plant growth parameters and reduced the nematode multiplication over untreated control. However, maximum plant growth parameters and minimum galls per root system, eggmasses per root system, eggs per egg masses and final nematode population was recorded when seeds of tomato (var. Pusa Ruby) was treated with talc formulation of Pseudomonus flourescens @20g/kg followed by seed treatment with vermi formulation of Bacillus subtilis @ 20g/kg of seed.

Effect of Oil Cakes and Bio-agents Each Alone and in Combination for Management of Root Knot Nematode (Meloidogyne incognita) in Green Gram

2021 ◽  
Vol 12 (5) ◽  
pp. 286-294
Author(s):  
Poornata Jena ◽  
◽  
N. K. Sahoo ◽  
J. K. Mahalik ◽  
◽  
...  
Keyword(s):  
Plant Growth ◽  
Chlorophyll Content ◽  
Meloidogyne Incognita ◽  
Seed Treatment ◽  
Growth Parameters ◽  
Green Gram ◽  
Root Knot Nematode ◽  
Neem Cake ◽  
Untreated Check ◽  
Am Fungus

A pot experiment was carried out in the net house of Department of Nematology, OUAT, Bhubaneswar, Odisha, India during June to August, 2017 on the application of oilcakes (mustard cake and neem cake) and bio-agents (Trichoderma viride, Glomus fasciculatum, Rhizobium leguminosarum) each alone and in combination for the management of root knot nematode (Meloidogyne incognita) in green gram. Result of the experiment indicated that soil application of mustard or neem cake @ 50 g m-2 with AM fungus (Glomus fasciculatum) @ 5 g m-² and seed treatment of Rhizobium @ 25 g kg-1 of green gram seed declined the root knot nematode population, number of galls plant-1, number of eggmass plant-1and root knot index with corresponding increase of plant growth parameters and chlorophyll content in green gram plant as compared to other treatments and untreated check. But integration of mustard cake @ 50 g m-2 at 2 weeks prior to sowing with AM fungus @ 5 g m-2 at 10 days before sowing and seed treatment of Rhizobium @ 25 g kg-1 green gram seed exhibited the lowest M. incognita population 200 cc soil-1 (153.33 J2), number of galls plant-1 (7.0), number of eggmass plant-1 (2.0) and root knot index (2.0) reflecting enhancement of plant growth parameters, number of pods (206.67%), number of nodules (691.17%) over untreated check. This integrated management module also recorded maximum increase in the availability of NPK content in soil and chlorophyll content as compared to other treatments.

scholarly journals Cladosporium sp. Isolate as Fungal Plant Growth Promoting Agent

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 392
Author(s):  
Iuliana Răut ◽  
Mariana Călin ◽  
Luiza Capră ◽  
Ana-Maria Gurban ◽  
Mihaela Doni ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Parameters ◽  
Hydrolytic Enzymes ◽  
Root Weight ◽  
Tomato Seedlings ◽  
Wide Range ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
And Performance ◽  
Positive Results

Cladosporium species are active in protecting plants against different biotic and abiotic stresses. Since these species produced a wide range of secondary metabolites responsible for the adaptation to new habitats, plant health and performance, they are of great interest, especially for biostimulants in agriculture. Cladosporium sp. produces protein hydrolysates (PHs), a class of biostimulants, by cultivation on medium with keratin wastes (feathers) as carbon and energy sources. The aim of this study was to select a Cladosporium isolate with potential to be used as plant growth promoting agent. The characteristics of Cladosporium isolates as plants biostimulants were evaluated through several tests, such as: antagonism versus plants pathogens, effect on plant growth of secreted volatiles produced by isolates, secretion of hydrolytic enzymes, production of 3-indole acetic acid, zinc and phosphorous solubilization, capacity to promote tomato seedlings growth (pot experiments). Cladosporium isolate T2 presented positive results to all tests. Encouraging results were obtained treating tomato seedlings with PHs from isolate Cladosporium T2 cultured on medium supplemented with 1% (w/w) chicken feathers, for which growth parameters, such as stem weight, stem height, and root weight were significantly higher by 65%, 32%, and 55%, respectively, compared to those treated with water.

scholarly journals Phytochemical management of root knot nematode (Meloidogyne incognita) kofoid and white chitwood by Artemisia spp. in tomato (Lycopersicon esculentum L.)

2020 ◽  
Vol 80 (4) ◽  
pp. 829-838 ◽  
Author(s):  
R. Khan ◽  
I. Naz ◽  
S. Hussain ◽  
R. A. A. Khan ◽  
S. Ullah ◽  
...  
Keyword(s):  
Lycopersicon Esculentum ◽  
Plant Growth ◽  
Meloidogyne Incognita ◽  
Growth Parameters ◽  
Negative Control ◽  
Root Knot Nematode ◽  
Egg Hatch ◽  
Egg Masses ◽  
Screen House

Abstract In vitro and screen house experiments were conducted to investigate the effectiveness of thirteen phytochemicals from Artemisia elegantissimia and A. incisa on root knot nematode, Meloidogyne incognita in tomato (Lycopersicon esculentum L.) cv. Rio Grande. A positive control (Carbofuran) and negative control (H2O) were also used for comparison. Effectiveness of phytochemicals against juveniles (J2s) mortality and egg hatch inhibition were evaluated after 24, 48 and 72 hours of incubation at three concentrations viz; 0.1, 0.2 and 0.3 mg/mL in vitro conditions. Amongst thirteen phytochemicals, Isoscopletin (Coumarin), Carbofuran and Apigenin (Flavonoid) showed the highest mortality and egg hatch inhibition of M. incognita at all intervals. Inhibition of eggs and J2s mortality were the greatest (90.0%) and (96.0%) at 0.3 mg/mL concentration. Application of phytochemicals caused reduction in number of galls, galling index, and egg masses on tomato plant and enhanced plant growth parameters under screen house conditions. Gall numbers (1.50), galling index (1.00), number of juveniles (4.83) and egg masses (4.00) were greatly reduced and plant growth parameters such as; plant height (28.48 cm), fresh (72.13 g) and dry shoot weights (35.99 g), and root fresh (6.58 g) and dry weights (1.43 g) were increased significantly by using Isoscopletin. In structure activity relationship, juveniles of M. incognita, exhibited variations in their shape and postures upon death when exposed to different concentrations of phytochemicals of Artemisia spp. The present study suggests that Artemisia based phytochemicals possess strong nematicidal effects and can be used effectively in an integrated disease management program against root knot nematodes.

scholarly journals Resistance screening of lentil cultivars against the root-knot nematode Meloidogyne incognita

2018 ◽  
Vol 11 (1) ◽  
pp. 9-18 ◽  
Author(s):  
T. Ansari ◽  
M. Asif ◽  
M.A. Siddiqui
Keyword(s):  
Plant Growth ◽  
Meloidogyne Incognita ◽  
Growth Parameters ◽  
Dry Weight ◽  
Fresh Weight ◽  
Root Knot Nematode ◽  
Nematode Reproduction ◽  
Egg Masses ◽  
Moderately Resistant ◽  
Reduction Percentage

SummaryThe root-knot nematodeMeloidogyne incognitais a major soil parasite of lentil crops. Increasing restrictions of chemical nematicides have triggered a growing attention and interest in alternate root-knot nematode management. The present study was conducted to examine the level of resistance and/or susceptibility of five lentil cultivars (PL-456, KLS-218, Desi, DPL-62, Malika), grown in pots, against the root-knot nematodeM. incognita. Root-knot nematode reproduction and host damage were assessed by recording the nematode infestation levels and reduction percentage of plant growth parameters. Nematode response and plant growth differentiated amongst the lentil cultivars. None of the cultivars was found immune or highly resistant. The cultivar Malika was found moderately resistant as it showed the lowest number of galls and egg masses/root as well as the lowest reduction of plant fresh weight (10.4%) and dry weight (6.9%). On the other hand, the cultivar Desi manifested the highest susceptibility exhibiting the highest number of galls and egg masses. There was a significantly negative correlation between the number of galls and plant growth parameters (plant fresh and dry weight and plant height).

scholarly journals Development of Meloidogyne incognita on selected okra cultivars

2019 ◽  
Vol 3 (2) ◽  
Author(s):  
Muhammad Usman Afzal ◽  
Sajid Aleem Khan ◽  
Nosheen Salehon ◽  
Mehwish Naz ◽  
Nasir Ahmad Khan
Keyword(s):  
Plant Growth ◽  
Meloidogyne Incognita ◽  
Growth Parameters ◽  
Vegetable Crop ◽  
Root Knot Nematode ◽  
Yield Attributes ◽  
Yield And Quality ◽  
Growth Attributes ◽  
Moderately Resistant ◽  
Nematode Development

Okra is a valuable vegetable crop of Pakistan. It is predominantly hosted by pathogens that reduce its yield and quality. Among these yield reducing agents, root knot nematodes are very important due to their host rang. Chemical control for this important pathogen is expensive and causes pollution problems so the present study was planned to evaluate the level of resistance among different available varieties of okra against Meloidogyne incognita under field conditions. The selected field was already infested with M. incognita. Data were recorded on various plant growth parameters and nematode development parameters after sixty days. Results indicated that for various nematode populations and plant growth attributes all the cultivars significantly differed with the Sabzpari 2001 and Sabzpari X Ramakrishna having best performance on over all yield by showing moderately resistant response but no variety was resistant against M. incognita. On over all basis and yield attributes, it is recommended that Sabzpari 2001 and Sabzpari X Ramakrishna are moderately resistant to nematode infestation and should be recommended to farmers in root knot nematode infested soils of Pakistan

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