scholarly journals Development of Meloidogyne incognita on selected okra cultivars

2019 ◽  
Vol 3 (2) ◽  
Author(s):  
Muhammad Usman Afzal ◽  
Sajid Aleem Khan ◽  
Nosheen Salehon ◽  
Mehwish Naz ◽  
Nasir Ahmad Khan
Keyword(s):  
Plant Growth ◽  
Meloidogyne Incognita ◽  
Growth Parameters ◽  
Vegetable Crop ◽  
Root Knot Nematode ◽  
Yield Attributes ◽  
Yield And Quality ◽  
Growth Attributes ◽  
Moderately Resistant ◽  
Nematode Development

Okra is a valuable vegetable crop of Pakistan. It is predominantly hosted by pathogens that reduce its yield and quality. Among these yield reducing agents, root knot nematodes are very important due to their host rang. Chemical control for this important pathogen is expensive and causes pollution problems so the present study was planned to evaluate the level of resistance among different available varieties of okra against Meloidogyne incognita under field conditions. The selected field was already infested with M. incognita. Data were recorded on various plant growth parameters and nematode development parameters after sixty days. Results indicated that for various nematode populations and plant growth attributes all the cultivars significantly differed with the Sabzpari 2001 and Sabzpari X Ramakrishna having best performance on over all yield by showing moderately resistant response but no variety was resistant against M. incognita. On over all basis and yield attributes, it is recommended that Sabzpari 2001 and Sabzpari X Ramakrishna are moderately resistant to nematode infestation and should be recommended to farmers in root knot nematode infested soils of Pakistan

scholarly journals Resistance screening of lentil cultivars against the root-knot nematode Meloidogyne incognita

2018 ◽  
Vol 11 (1) ◽  
pp. 9-18 ◽  
Author(s):  
T. Ansari ◽  
M. Asif ◽  
M.A. Siddiqui
Keyword(s):  
Plant Growth ◽  
Meloidogyne Incognita ◽  
Growth Parameters ◽  
Dry Weight ◽  
Fresh Weight ◽  
Root Knot Nematode ◽  
Nematode Reproduction ◽  
Egg Masses ◽  
Moderately Resistant ◽  
Reduction Percentage

SummaryThe root-knot nematodeMeloidogyne incognitais a major soil parasite of lentil crops. Increasing restrictions of chemical nematicides have triggered a growing attention and interest in alternate root-knot nematode management. The present study was conducted to examine the level of resistance and/or susceptibility of five lentil cultivars (PL-456, KLS-218, Desi, DPL-62, Malika), grown in pots, against the root-knot nematodeM. incognita. Root-knot nematode reproduction and host damage were assessed by recording the nematode infestation levels and reduction percentage of plant growth parameters. Nematode response and plant growth differentiated amongst the lentil cultivars. None of the cultivars was found immune or highly resistant. The cultivar Malika was found moderately resistant as it showed the lowest number of galls and egg masses/root as well as the lowest reduction of plant fresh weight (10.4%) and dry weight (6.9%). On the other hand, the cultivar Desi manifested the highest susceptibility exhibiting the highest number of galls and egg masses. There was a significantly negative correlation between the number of galls and plant growth parameters (plant fresh and dry weight and plant height).

Effect of Oil Cakes and Bio-agents Each Alone and in Combination for Management of Root Knot Nematode (Meloidogyne incognita) in Green Gram

2021 ◽  
Vol 12 (5) ◽  
pp. 286-294
Author(s):  
Poornata Jena ◽  
◽  
N. K. Sahoo ◽  
J. K. Mahalik ◽  
◽  
...  
Keyword(s):  
Plant Growth ◽  
Chlorophyll Content ◽  
Meloidogyne Incognita ◽  
Seed Treatment ◽  
Growth Parameters ◽  
Green Gram ◽  
Root Knot Nematode ◽  
Neem Cake ◽  
Untreated Check ◽  
Am Fungus

A pot experiment was carried out in the net house of Department of Nematology, OUAT, Bhubaneswar, Odisha, India during June to August, 2017 on the application of oilcakes (mustard cake and neem cake) and bio-agents (Trichoderma viride, Glomus fasciculatum, Rhizobium leguminosarum) each alone and in combination for the management of root knot nematode (Meloidogyne incognita) in green gram. Result of the experiment indicated that soil application of mustard or neem cake @ 50 g m-2 with AM fungus (Glomus fasciculatum) @ 5 g m-² and seed treatment of Rhizobium @ 25 g kg-1 of green gram seed declined the root knot nematode population, number of galls plant-1, number of eggmass plant-1and root knot index with corresponding increase of plant growth parameters and chlorophyll content in green gram plant as compared to other treatments and untreated check. But integration of mustard cake @ 50 g m-2 at 2 weeks prior to sowing with AM fungus @ 5 g m-2 at 10 days before sowing and seed treatment of Rhizobium @ 25 g kg-1 green gram seed exhibited the lowest M. incognita population 200 cc soil-1 (153.33 J2), number of galls plant-1 (7.0), number of eggmass plant-1 (2.0) and root knot index (2.0) reflecting enhancement of plant growth parameters, number of pods (206.67%), number of nodules (691.17%) over untreated check. This integrated management module also recorded maximum increase in the availability of NPK content in soil and chlorophyll content as compared to other treatments.

Resistance in Mungbean Against Meloidogyne incognita and its Impact on Nodulation

2020 ◽  
Author(s):  
Narpinderjeet Kaur Dhillon ◽  
Rohit Kumar ◽  
Sukhjeet Kaur ◽  
Anupam Anupam ◽  
Asmita Srari
Keyword(s):  
Plant Growth ◽  
Growth Parameters ◽  
Root Knot Nematode ◽  
Sources Of Resistance ◽  
Kharif Season ◽  
Breeding Programmes ◽  
Meloidogyne Spp ◽  
Moderately Resistant ◽  
The Impact ◽  
Resistant Genotypes

Mungbean is an economically as well as nutritionally enriched crop. Of the different soil borne pathogens attacking mungbean, root-knot nematode (Meloidogyne spp.) is an important pathogen affecting growth and production of mungbean. It is grown in summer as well as in kharif season. The germplasm of mungbean of two seasons’ viz., summer and kharif was screened to identify new sources of resistance against root knot nematode, M. incognita. In addition to screening; studies were also conducted on the impact of root knot nematode infestation in roots on nodulation character of mungbean and growth parameters. Of the sixty three genotypes evaluated in summer, seven were found to be moderately resistant. In kharif season, only three genotypes were found to be moderately resistant. M. incognita infestation was also observed to affect the plant growth parameters as well as nodulation on roots of mungbean genotypes. Comparatively, better plant growth and higher nodulation was observed in moderately resistant genotypes as compared to the susceptible ones. The ten identified moderately resistant genotypes from two seasons can be a useful source in breeding programmes for developing cultivars to manage root knot nematode.

scholarly journals Screening of cucumber (Cucumis sativus L.) cultivars against southern root knot nematode, Meloidogyne incognita (Kofoid and White) Chitwood

2020 ◽  
Vol 49 (3) ◽  
pp. 579-584
Author(s):  
Ifra Siddique ◽  
Ishrat Naz ◽  
Raja Asad Ali Khan ◽  
Musharaf Ahmed ◽  
Syeda Maryam Hussain
Keyword(s):  
Population Density ◽  
Meloidogyne Incognita ◽  
Growth Parameters ◽  
High Population Density ◽  
In Planta ◽  
Root Knot Nematode ◽  
Cucumis Sativus L ◽  
Southern Root Knot Nematode ◽  
Moderately Resistant ◽  
Significant Mortality

Fourteen cultivars of cucumber were screened for their resistance to the Southern root-knot nematode, Meloidogyne incognita (Kofoid and White) Chitwood in an in planta experiment. The pots were maintained in greenhouse with CRD for 50 days after inoculation. The cultivar DS92-05 induced significant mortality and was rated “moderately resistant (MR)”. This cultivar showed increase in plant growth parameters including vine length. The cultivars DS92-06, Laghman, Sultan and Desitype were moderately susceptible (MS). The cultivar Rehan and DS96-299 were rated susceptible (S) whereas DS97-299, Chaiya, Beitalpha, Alto, DS92-04 and Local were rated as highly susceptible (HS). DS92-05 is thus promising for sustainable agriculture, specially in those areas with high population density of Southern Root knot nematode.

scholarly journals Reducing RbcS Expression by RNAi Technology Causes Diverse Responses to Nematode in Wheat

2019 ◽  
Vol 22 (02) ◽  
pp. 395-400
Author(s):  
Saqer Alotaibi
Keyword(s):  
Plant Growth ◽  
Growth Parameters ◽  
Dry Weight ◽  
Root Knot Nematode ◽  
Susceptible Host ◽  
Nematode Reproduction ◽  
Shoot Dry Weight ◽  
Rnai Technology ◽  
Wheat Lines ◽  
Nematode Development

The reaction of four wheat RbcS RNAi mutants and wild type to the infection of M. incognita was investigated in a greenhouse pot experiment using plant growth parameters as well as nematode reproduction. Obtained results revealed that all estimated growth parameters of lines under study were reduced by M. incognita infection to various extents. RSS13.2 line showed the highest percentage of reduction of plant total fresh weight, length, and shoot dry weight. It did act as good host for root-knot nematode; therefore, it was classified as susceptible host. In addition, the lowest reduction percentage of fresh and dry weights was observed in RSS8.5 line although it showed high values for nematode development and reproduction parameters; therefore, it seemed to be as a tolerant host. The maximum reduction in chlorophyll content was recorded in RSS7.6 line, whereas, the minimum was obtained from RSS11.5 line. It was evident that none of the five tested wheat lines was immune to M. incognita infection. The lowest numbers of second stage juveniles, galls, and egg-masses were observed in soil and on roots of RSS11.5 line although its plant growth was slightly affected; therefore, it is considered as partially resistant line. It could be concluded from the results of this study that, RNAi mutants of the RbcS were partially effective in suppressing root-knot nematode development. © 2019 Friends Science Publishers

scholarly journals Phytochemical management of root knot nematode (Meloidogyne incognita) kofoid and white chitwood by Artemisia spp. in tomato (Lycopersicon esculentum L.)

2020 ◽  
Vol 80 (4) ◽  
pp. 829-838 ◽  
Author(s):  
R. Khan ◽  
I. Naz ◽  
S. Hussain ◽  
R. A. A. Khan ◽  
S. Ullah ◽  
...  
Keyword(s):  
Lycopersicon Esculentum ◽  
Plant Growth ◽  
Meloidogyne Incognita ◽  
Growth Parameters ◽  
Negative Control ◽  
Root Knot Nematode ◽  
Egg Hatch ◽  
Egg Masses ◽  
Screen House

Abstract In vitro and screen house experiments were conducted to investigate the effectiveness of thirteen phytochemicals from Artemisia elegantissimia and A. incisa on root knot nematode, Meloidogyne incognita in tomato (Lycopersicon esculentum L.) cv. Rio Grande. A positive control (Carbofuran) and negative control (H2O) were also used for comparison. Effectiveness of phytochemicals against juveniles (J2s) mortality and egg hatch inhibition were evaluated after 24, 48 and 72 hours of incubation at three concentrations viz; 0.1, 0.2 and 0.3 mg/mL in vitro conditions. Amongst thirteen phytochemicals, Isoscopletin (Coumarin), Carbofuran and Apigenin (Flavonoid) showed the highest mortality and egg hatch inhibition of M. incognita at all intervals. Inhibition of eggs and J2s mortality were the greatest (90.0%) and (96.0%) at 0.3 mg/mL concentration. Application of phytochemicals caused reduction in number of galls, galling index, and egg masses on tomato plant and enhanced plant growth parameters under screen house conditions. Gall numbers (1.50), galling index (1.00), number of juveniles (4.83) and egg masses (4.00) were greatly reduced and plant growth parameters such as; plant height (28.48 cm), fresh (72.13 g) and dry shoot weights (35.99 g), and root fresh (6.58 g) and dry weights (1.43 g) were increased significantly by using Isoscopletin. In structure activity relationship, juveniles of M. incognita, exhibited variations in their shape and postures upon death when exposed to different concentrations of phytochemicals of Artemisia spp. The present study suggests that Artemisia based phytochemicals possess strong nematicidal effects and can be used effectively in an integrated disease management program against root knot nematodes.

scholarly journals Efficacy of Some Bacterial Biocontrol Agents as Seed Treatment against Root Knot Nematode, Meloidogyne incognita on Tomato

2020 ◽  
Vol 9 (9) ◽  
pp. 1043-1046
Author(s):  
Karter Nyodu ◽  
Debanand Das
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Root System ◽  
Bacillus Megaterium ◽  
Meloidogyne Incognita ◽  
Seed Treatment ◽  
Bacillus Pumilus ◽  
Growth Parameters ◽  
Biocontrol Agents ◽  
Root Knot Nematode

Pot experiment was conducted to evaluate the efficacy of two formulations (talc formulation and vermi formulation) of four bacterial nematode biocontrol agents viz., Bacillus subtilis, Bacillus pumilus, Bacillus megaterium and Pseudomonus flourescens as seed treatment against root knot nematode, Meloidogyne incognita on tomato. All the bioagents were tries at 10 and 20g / kg of seed. The experiment was terminated at 60 days after sowing the seed. The results revealed that all the treatments significantly increased the plant growth parameters and reduced the nematode multiplication over untreated control. However, maximum plant growth parameters and minimum galls per root system, eggmasses per root system, eggs per egg masses and final nematode population was recorded when seeds of tomato (var. Pusa Ruby) was treated with talc formulation of Pseudomonus flourescens @20g/kg followed by seed treatment with vermi formulation of Bacillus subtilis @ 20g/kg of seed.

scholarly journals Management of root-knot nematode Meloidogyne incognita of eggplant using some growth-promoting rhizobacteria and chitosan under greenhouse conditions

2020 ◽  
Vol 30 (1) ◽  
Author(s):  
Usama Samy Elkelany ◽  
Nehal Samy El-Mougy ◽  
Mokhtar Mohamed Abdel-Kader
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Meloidogyne Incognita ◽  
Growth Parameters ◽  
Azotobacter Chroococcum ◽  
Economic Losses ◽  
Egg Mass ◽  
Root Knot Nematode ◽  
Wide Range ◽  
Growth Promoting

Abstract Background Eggplant (Solanum melongena) is one of the most popular vegetable crops in Egypt. It is affected by a wide range of pests; prominent among them is root-knot nematode (RKN) Meloidogyne spp. which constitutes a major group of plant-parasitic nematodes causing great economic losses worldwide especially in Egypt. Main body The present investigation was performed under greenhouse conditions to evaluate the potentials of 3 growth-promoting bacteria, Azospirilum brasilense, Azotobacter chroococcum, and Bacillus subtilis, as well as chitosan for the control of the nematode Meloidogyne incognita causes root-knot disease of eggplant cv. Baladi. All treatments reduced (p ≤ 0.05) the nematode population in soil and roots as well as enhanced the plant growth parameters of eggplant remarkably than the control. The applied treatments varied in their efficacy against the plant nematode infection in correspondence to the time of application. The recorded results demonstrated that maximum reduction in J2 in soil, egg mass/root, and eggs/egg masses were obtained by treating the soil with Bacillus subtilis followed by Azospirilum brasilense and Azotobacter chroococcum. Also, chitosan resulted in high reduction in root galls compared to control treatment. Conclusion The use of plant growth-promoting rhizobacteria, Azospirilum brasilense, Azotobacter chroococcum, Bacillus subtilis, and chitosan achieved efficient control to Meloidogyne incognita and consequently increase eggplant growth parameters under greenhouse conditions. The present results suggested introducing such rhizobacteria in integrated nematode management program.

scholarly journals Management of Root Knot Nematode Meloidogyne incognita in Banana (Cv. Robusta) through Biocontrol Agents and Neem Cake

2020 ◽  
Author(s):  
P. Saravanan ◽  
N. Ilavarasan ◽  
A. Karthikeyan ◽  
B. Padmanaban
Keyword(s):  
Plant Growth ◽  
Meloidogyne Incognita ◽  
Growth Parameters ◽  
Trichoderma Viride ◽  
Control Plant ◽  
Biocontrol Agents ◽  
Root Knot Nematode ◽  
Banana Plant ◽  
Neem Cake ◽  
Gm Plant

A pot culture experiment was performed to study the effect of native promising biocontrol agents and neem cake separately and in combination against root-knot nematode Meloidogyne incognita infecting banana cv. Robusta during 2016-2017. Treatments treated with bioagents and neem cake enhanced plant growth and root characters and suppressed root gall development in banana plant as compared to nematode alone and untreated control treatments. Among the various treatments, the combined application of Pseudomonas fluorescens @ 10 g/plant + Trichoderma viride @ 10 gm/plant + neem cake @ 100 gm/plant resulted in maximum increase of plant height (38.8 cm), number of leaves (7), pseudostem girth (15 cm), root length (25.6 cm), highest number of healthy roots (17.0), poor number of infected roots (3.3), lowest root gall index (1 scale) and significantly reduced root-knot nematode population both soil (55.0 nos) and roots (90.0 nos). The application of single bioagent either P.fluorescens @ 10 gm/plant or T.viride @ 10 gm/plant along with neem cake were also recorded highest plant growth parameters and poor nematode infestation when compared to nematode alone treated control plant.

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