scholarly journals Impact of endometrial microbiome on fertility

2021 ◽  
Vol 26 (1) ◽  
Author(s):  
Aboubakr Mohamed Elnashar
Keyword(s):  
Pregnancy Loss ◽  
Reproductive Tract ◽  
Clinical Care ◽  
Female Reproductive Tract ◽  
Main Body ◽  
Implantation Failure ◽  
Unculturable Bacteria ◽  
The Impact ◽  
Generation Sequencing ◽  
Infertile Patients

Abstract Background Next-generation sequencing isolates culturable and unculturable bacteria from the female reproductive tract. Current literatures surrounding the impact of endometrial microbiome on fertility are reviewed. Main body An abnormal endometrial microbiota has been associated with implantation failure pregnancy loss and other gynecological and obstetrical conditions. Identification of endometrial dysbiosis as a new cause of infertility opens a new microbiological field in the evaluation of endometrial factor, highlighting the relevance of assessing the uterine microbiota in infertile patients to restore a favorable endometrial flora in those patients with altered uterine microbiota to improve and personalize the clinical care of infertile patients. Understanding the significance of microbiome in the endometrium may completely change the therapeutic approach in the treatment of this part of the reproductive tract. Conclusion Investigation of the endometrial microbiota may be a future tool for improving reproductive outcomes in infertile patients. Further well-designed studies are required to establish its role in the evaluation and treatment of infertile patients

scholarly journals Proteomics and metabolomics in cow fertility: a systematic review

Reproduction ◽  
2020 ◽  
Vol 160 (5) ◽  
pp. 639-658
Author(s):  
Nicolas Aranciaga ◽  
James D Morton ◽  
Debra K Berg ◽  
Jessica L Gathercole
Keyword(s):  
Oxidative Stress ◽  
Systematic Review ◽  
Large Scale ◽  
Reproductive Tract ◽  
Early Embryo ◽  
Female Reproductive Tract ◽  
Full Potential ◽  
Technical Standards ◽  
The Impact

Cow subfertility is a multi-factorial problem in many countries which is only starting to be unravelled. Molecular biology can provide a substantial source of insight into its causes and potential solutions, particularly through large scale, untargeted omics approaches. In this systematic review, we set out to compile, assess and integrate the latest proteomic and metabolomic research on cow reproduction, specifically that on the female reproductive tract and early embryo. We herein report a general improvement in technical standards throughout the temporal span examined; however, significant methodological limitations are also identified. We propose easily actionable avenues for ameliorating these shortcomings and enhancing the reach of this field. Text mining and pathway analysis corroborate the relevance of proteins and metabolites related to the triad oxidative stress-inflammation-disease on reproductive function. We envisage a breakthrough in cattle reproductive molecular research within the next few years as in vivo sample techniques are improved, omics analysis equipment becomes more affordable and widespread, and software tools for single- and multi-omics data processing are further developed. Additional investigation of the impact of local oxidative stress and inflammation on fertility, both at the local and systemic levels, is key towards realising the full potential of this field.

scholarly journals Endometrial microbiota composition is associated with reproductive outcome in infertile patients

2021 ◽  
Author(s):  
Inmaculada Moreno ◽  
Iolanda Garcia-Grau ◽  
David Perez-Villaroya ◽  
Marta Gonzalez-Monfort ◽  
Mustafa Bahçeci ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Live Birth ◽  
Reproductive Tract ◽  
Treatment Strategies ◽  
16S Rrna Gene Sequencing ◽  
Female Reproductive Tract ◽  
Rrna Gene ◽  
Microbiota Composition ◽  
Reproductive Outcome ◽  
Infertile Patients

ABSTRACTBackgroundPrevious evidence indicates associations between the female reproductive tract microbiome composition and reproductive outcome in infertile patients undergoing assisted reproduction. We aimed to determine whether the endometrial microbiota composition is associated with reproductive outcomes of live birth, biochemical pregnancy, clinical miscarriage, or no pregnancy.MethodsHere we present a multicentre prospective observational study using 16S rRNA gene sequencing to analyse endometrial fluid and biopsy samples before embryo transfer in a cohort of 342 infertile patients asymptomatic for infection undergoing assisted reproductive treatments.ResultsA dysbiotic endometrial microbiota profile composed of Atopobium, Bifidobacterium, Chryseobacterium, Gardnerella, Haemophilus, Klebsiella, Neisseria, Staphylococcus and Streptococcus was associated with unsuccessful outcomes. In contrast, Lactobacillus was consistently enriched in patients with live birth outcomes.ConclusionsOur findings indicate that endometrial microbiota composition before embryo transfer is a useful biomarker to predict reproductive outcome, offering an opportunity to further improve diagnosis and treatment strategies.

scholarly journals Increased FOXL2 Expression Alters Uterine Structures and Functions

2020 ◽  
Author(s):  
Rong Li ◽  
San-Pin Wu ◽  
Lecong Zhou ◽  
Barbara Nicol ◽  
John P. Lydon ◽  
...  
Keyword(s):  
Reproductive Tract ◽  
Sex Differentiation ◽  
Reproductive Function ◽  
Endometrial Biopsy ◽  
Female Reproductive Tract ◽  
Mrna Levels ◽  
Over Expression ◽  
Ovariectomized Mice ◽  
The Impact

AbstractTranscription factor FOXL2 exhibits an increase in mRNA levels in eutopic endometrial biopsy in endometriosis patients. While FOXL2 is known of regulating sex differentiation and reproductive function, the impact of elevated FOXL2 expression on uterine physiology remains unknown. To answer this question, we generated mice with over expression of FOXL2 (FOXL2OE) in the female reproductive tract by crossing Foxl2LsL/+ with the Pgrcre model. FOXL2OE uterus showed severe morphological abnormality including abnormal epithelial stratification, blunted adenogenesis, increased endometrial fibrosis and disrupted myometrial morphology. In contrast, increasing FOXL2 levels specifically in uterine epithelium by crossing the Foxl2LsL/+ with the Ltficre mice resulted in the eFOXL2OE mice with uterine epithelial stratification but without defects in endometrial fibrosis and adenogenesis, demonstrating a role of the endometrial stroma in the uterine abnormalities of the FOXL2OE mice. Transcriptomic analysis of 12 weeks old Pgrcre and FOXL2OE uterus at diestrus stage showed a positive correlation of FOXL2OE uterine transcriptome with human endometrium of endometriosis patients. Furthermore, we found FOXL2OE mice were sterile. The infertility was caused in part by a disruption of the hypophyseal ovarian axis resulting in an anovulatory phenotype. The FOXL2OE mice failed to show decidual responses during artificial decidualization in ovariectomized mice which demonstrates the uterine contribution to the infertility phenotype. These data supported that aberrantly increased FOXL2 expressions in the female reproductive tract can disrupt ovarian and uterine functions, particularly, may be involved in the progressions of endometriosis.

scholarly journals Endometrial microbiota composition is associated with reproductive outcome in infertile patients

Microbiome ◽  
2022 ◽  
Vol 10 (1) ◽  
Author(s):  
Inmaculada Moreno ◽  
Iolanda Garcia-Grau ◽  
David Perez-Villaroya ◽  
Marta Gonzalez-Monfort ◽  
Mustafa Bahçeci ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Live Birth ◽  
Reproductive Tract ◽  
Treatment Strategies ◽  
16S Rrna Gene Sequencing ◽  
Female Reproductive Tract ◽  
Rrna Gene ◽  
Microbiota Composition ◽  
Reproductive Outcome ◽  
Infertile Patients

Abstract Background Previous evidence indicates associations between the female reproductive tract microbiome composition and reproductive outcome in infertile patients undergoing assisted reproduction. We aimed to determine whether the endometrial microbiota composition is associated with reproductive outcomes of live birth, biochemical pregnancy, clinical miscarriage or no pregnancy. Methods Here, we present a multicentre prospective observational study using 16S rRNA gene sequencing to analyse endometrial fluid and biopsy samples before embryo transfer in a cohort of 342 infertile patients asymptomatic for infection undergoing assisted reproductive treatments. Results A dysbiotic endometrial microbiota profile composed of Atopobium, Bifidobacterium, Chryseobacterium, Gardnerella, Haemophilus, Klebsiella, Neisseria, Staphylococcus and Streptococcus was associated with unsuccessful outcomes. In contrast, Lactobacillus was consistently enriched in patients with live birth outcomes. Conclusions Our findings indicate that endometrial microbiota composition before embryo transfer is a useful biomarker to predict reproductive outcome, offering an opportunity to further improve diagnosis and treatment strategies.

Uterine microbiome—low biomass and high expectations†

2018 ◽  
Vol 101 (6) ◽  
pp. 1102-1114 ◽  
Author(s):  
Iolanda Garcia-Grau ◽  
Carlos Simon ◽  
Inmaculada Moreno
Keyword(s):  
Bacterial Communities ◽  
Sampling Method ◽  
Reproductive Tract ◽  
Female Reproductive Tract ◽  
High Expectations ◽  
Downstream Analysis ◽  
Uterine Diseases ◽  
Human Fetal Development ◽  
Infertile Patients

AbstractThe existence of different bacterial communities throughout the female reproductive tract has challenged the traditional view of human fetal development as a sterile event. There is still no consensus on what physiological microbiota exists in the upper reproductive tract of the vast majority of women who are not in periods of infection or pregnancy, and the role of bacteria that colonize the upper reproductive tract in uterine diseases or pregnancy outcomes is not well established. Despite published studies and advances in uterine microbiome sequencing, some study aspects—such as study design, sampling method, DNA extraction, sequencing methods, downstream analysis, and assignment of taxa—have not yet been improved and standardized. It is time to further investigate the uterine microbiome to increase our understanding of the female reproductive tract and to develop more personalized reproductive therapies, highlighting the potential importance of using microbiological assessment in infertile patients.

scholarly journals Discovery of Novel MicroRNAs in Female Reproductive Tract Using Next Generation Sequencing

PLoS ONE ◽  
2010 ◽  
Vol 5 (3) ◽  
pp. e9637 ◽  
Author(s):  
Chad J. Creighton ◽  
Ashley L. Benham ◽  
Huifeng Zhu ◽  
Mahjabeen F. Khan ◽  
Jeffrey G. Reid ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Reproductive Tract ◽  
Female Reproductive Tract ◽  
Next Generation ◽  
Generation Sequencing

scholarly journals The Female Reproductive Tract Microbiome—Implications for Gynecologic Cancers and Personalized Medicine

2021 ◽  
Vol 11 (6) ◽  
pp. 546
Author(s):  
Anthony E. Rizzo ◽  
Jennifer C. Gordon ◽  
Alicia R. Berard ◽  
Adam D. Burgener ◽  
Stefanie Avril
Keyword(s):  
Personalized Medicine ◽  
Reproductive Tract ◽  
Uterine Cavity ◽  
Convincing Evidence ◽  
Female Reproductive Tract ◽  
Microbial Colonization ◽  
Future Research ◽  
Gynecologic Cancers ◽  
And Personalized Medicine ◽  
Generation Sequencing

The microbial colonization of the lower female reproductive tract has been extensively studied over the past few decades. In contrast, the upper female reproductive tract including the uterine cavity and peritoneum where the ovaries and fallopian tubes reside were traditionally assumed to be sterile under non-pathologic conditions. However, recent studies applying next-generation sequencing of the bacterial 16S ribosomal RNA gene have provided convincing evidence for the existence of an upper female reproductive tract microbiome. While the vaginal microbiome and its importance for reproductive health outcomes has been extensively studied, the microbiome of the upper female reproductive tract and its relevance for gynecologic cancers has been less studied and will be the focus of this article. This targeted review summarizes the pertinent literature on the female reproductive tract microbiome in gynecologic malignancies and its anticipated role in future research and clinical applications in personalized medicine.

scholarly journals Clinical application of cell-free next-generation sequencing for infectious diseases at a tertiary children’s hospital

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Julianne Wilke ◽  
Nanda Ramchandar ◽  
Christopher Cannavino ◽  
Alice Pong ◽  
Adriana Tremoulet ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Infectious Diseases ◽  
Clinical Application ◽  
Clinical Care ◽  
Next Generation ◽  
Children's Hospital ◽  
Percent Agreement ◽  
Children’S Hospital ◽  
The Impact ◽  
Generation Sequencing

Abstract Background Children affected by infectious diseases may not always have a detectable infectious etiology. Diagnostic uncertainty can lead to prolonged hospitalizations, inappropriately broad or extended courses of antibiotics, invasive diagnostic procedures, and difficulty predicting the clinical course and outcome. Cell-free plasma next-generation sequencing (cfNGS) can identify viral, bacterial, and fungal infections by detecting pathogen DNA in peripheral blood. This testing modality offers the ability to test for many organisms at once in a shotgun metagenomic approach with a rapid turnaround time. We sought to compare the results of cfNGS to conventional diagnostic test results and describe the impact of cfNGS on clinical care in a diverse pediatric population at a large academic children’s hospital. Methods We performed a retrospective chart review of hospitalized subjects at a tertiary pediatric hospital to determine the diagnostic yield of cfNGS and its impact on clinical care. Results We describe the clinical application of results from 142 cfNGS tests in the management of 110 subjects over an 8-month study period. In comparison to conventional testing as a reference standard, cfNGS was found to have a positive percent agreement of 89.6% and negative percent agreement of 52.3%. Furthermore, 32.4% of cfNGS results were directly applied to make a clinical change in management. Conclusions We demonstrate the clinically utility of cfNGS in the management of acutely ill children. Future studies, both retrospective and prospective, are needed to clarify the optimal indications for testing.

scholarly journals To understand the reproductive tract microbiome associated with infertility through metagenomics analysis

2021 ◽  
Vol 26 (1) ◽  
Author(s):  
Manisha Vajpeyee ◽  
Lokendra Bahadur Yadav ◽  
Shivam Tiwari ◽  
Parikshit Tank
Keyword(s):  
Reproductive Tract ◽  
Assisted Reproductive Technologies ◽  
Live Birth Rate ◽  
Reproductive Technologies ◽  
Female Reproductive Tract ◽  
Human Reproduction ◽  
Main Body ◽  
Fertility Treatments ◽  
Vaginal Tract ◽  
Human Illness

Abstract Background Knowledge of the microbiome is in its infancy in health and human illness, especially concerning human reproduction. We will be better able to treat dysbiosis of the reproductive tract clinically if it is better explained and understood. It has been shown that altered vaginal microbiota affects parturition, and its function is uncertain in assisted reproductive technologies. However, the effects of recognized microbes such as Mycoplasma tuberculosis, Chlamydia trachomatis, and Neisseria gonorrhoeae are well established, resulting in subclinical changes which are considered to be risk factors for infertility and poor reproductive outcomes. Main body Recent studies indicate that the vaginal tract comprises several different organisms of the microbiome. Some microbiota can play an important role not only in the reproductive tract but also in overall health. The microbiome of the female reproductive tract has been identified mainly based on studies that examine vaginal samples across many reproductive technologies, using a metagenomics approach. Conclusion Alteration of reproductive tract microbiota or presence of certain microbiota irrespective of the level of pathogenicity may interfere with fertilization, implantation, and subsequent embryo development. This may lead to failed fertility treatments and reduced live birth rate (LBR).

Sign in / Sign up

Export Citation Format

Share Document