Micro RNA-23b as a potential biomarker in rheumatoid arthritis disease activity and severity: clinical, laboratory, and radiological cross-sectional study

Abstract Background Rheumatoid arthritis (RA) is an autoimmune inflammatory disease. It is characterized by an inflammatory polyarthritis that preferentially affects the small joints leading to joint damage and eventual deformity and disability, and can also present with extra-articular manifestations. Micro RNA (miRNA) is a class of non-coding RNAs which negatively regulate messenger RNA (mRNA) expression. Several studies had shown that miRNA-23b has a close relationship with inflammation and autoimmune diseases. An increasing evidence has suggested that miRNA-23b is closely associated with many inflammatory and autoimmune diseases. The current study aimed to evaluate the plasma expression of miRNA-23b in rheumatoid arthritis (RA) patients and to explore its potential association with diseases activity. Results RA patients had a significantly higher plasma miRNA-23b expression than controls (P < 0.001). The miRNA-23b plasma expression was significantly associated with the clinical and laboratory indices of RA activity as well as with the DAS28-ESR score (P = 0.009) and grades (P < 0.001). The miRNA-23b plasma expression was significantly correlated with the radiological severity of RA (P = 0.002). Conclusions Plasma expression of miRNA-23b is significantly increased in patients with RA than controls. In RA patients, plasma expression of miRNA-23b was significantly correlated with the activity and radiological severity of RA. miRNA-23b may represent a potential therapeutic target that can retard progression of RA.

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Serum adiponectin and its impact on disease activity and radiographic joint damage in early rheumatoid arthritis – A cross-sectional study

Indian Journal of Rheumatology ◽

10.1016/j.injr.2016.01.006 ◽

2016 ◽

Cited By ~ 1

Author(s):

Srinivasa Chennareddy ◽

Kurimeti V. Kishore Babu ◽

Sirisha Kommireddy ◽

Rajendra Varaprasad ◽

Liza Rajasekhar

Keyword(s):

Rheumatoid Arthritis ◽

Disease Activity ◽

Early Rheumatoid Arthritis ◽

Joint Damage ◽

Cross Sectional Study ◽

Serum Adiponectin ◽

Sectional Study ◽

Cross Sectional

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No Correlation Exists between Disease Activity and the Expression of Killer-Cell Immunoglobulin-Like Receptors in Patients with Rheumatoid Arthritis

Mediators of Inflammation ◽

10.1155/2007/65179 ◽

2007 ◽

Vol 2007 ◽

pp. 1-4 ◽

Cited By ~ 1

Author(s):

Toshiaki Kogure ◽

Takeshi Tatsumi ◽

Atsushi Niizawa ◽

Hiroshi Fujinaga ◽

Tomoyuki Ito ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Disease Activity ◽

Mononuclear Cells ◽

Killer Cell ◽

Joint Damage ◽

Cross Sectional Study ◽

Remarkable Change ◽

Peripheral Blood Mononuclear ◽

Cross Sectional ◽

Inflammation Parameters

Objective. The genes for killer-cell immunoglobulin-like receptors (KIRs) have been cloned and their functions and expression in patients with rheumatoid arthritis (RA) have been partially clarified. However, the correlation between their expression and disease activity has not been analyzed in patients with RA. Thus, we measured KIR expression on lymphocytes in patients with RA, and assessed the correlation between KIR expression and disease activity.Patients and Methods. In the cross-sectional study, 15 patients (9 females and 6 males) who fulfilled the diagnostic criteria for RA were assessed. In the longitudinal study, patients who were followed-up for 3 months were assessed. CD158a/b expression on peripheral blood mononuclear cells (PBMC) of RA patients was analyzed using flow cytometry.Results. No significant correlation between KIR expression and CRP, ESR, or IgM-RF was observed. There was no remarkable change in the expression of KIRs between the baseline and after 3 months. Additionally, in the 5 patients whose expression of KIRs particularly changed, the time-related changes in the expression of KIRs were independent from those of inflammation parameters and IgM-RF.Conclusion. There was no correlation between KIR expression and disease activity; therefore, the clinical use of KIR expression should be limited, while unnatural KIR expression may be involved in the pathogenesis of RA, but not a recruitment of chronic inflammation to induce joint damage.

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Spondyloarthropathies in Autoimmune Diseases and Vice Versa

Autoimmune Diseases ◽

10.1155/2012/736384 ◽

2012 ◽

Vol 2012 ◽

pp. 1-7 ◽

Cited By ~ 1

Author(s):

Oscar M. Pérez-Fernández ◽

Rubén D. Mantilla ◽

Paola Cruz-Tapias ◽

Alberto Rodriguez-Rodriguez ◽

Adriana Rojas-Villarraga ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Systemic Lupus Erythematosus ◽

Autoimmune Diseases ◽

Lupus Erythematosus ◽

Clinical Characteristics ◽

Cross Sectional Study ◽

Sectional Study ◽

Systemic Lupus ◽

Two Phase ◽

Cross Sectional

Polyautoimmunity is one of the major clinical characteristics of autoimmune diseases (ADs). The aim of this study was to investigate the prevalence of ADs in spondyloarthropathies (SpAs) and vice versa. This was a two-phase cross-sectional study. First, we examined the presence of ADs in a cohort of patients with SpAs (N=148). Second, we searched for the presence of SpAs in a well-defined group of patients with ADs (N=1077) including rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), and Sjögren’s syndrome (SS). Among patients with SpAs, ankylosing spondylitis was observed in the majority of them (55.6%). There were two patients presenting with SS in the SpA group (1.4%) and 5 patients with autoimmune thyroiditis (3.5%). The global prevalence of ADs in SpAs was 4.86%. In the ADs group, there were 5 patients with SpAs (0.46%). Our results suggest a lack of association between SpAs and ADs. Accordingly, SpAs might correspond more to autoinflammatory diseases rather than to ADs.

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Contribution of Ultrasonography of hands and wrists in Early Rheumatoid Arthritis

Current Rheumatology Reviews ◽

10.2174/1573397117666210219120400 ◽

2021 ◽

Vol 17 ◽

Author(s):

Saoussen Miladi ◽

Wafa Hamdi ◽

Kaouther Maatallah ◽

Asma el Elj ◽

Mouna Chelli ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Clinical Examination ◽

Clinical Laboratory ◽

Cross Sectional Study ◽

Clinical Findings ◽

Added Value ◽

Radiographic Examination ◽

Cross Sectional ◽

Musculoskeletal Ultrasonography ◽

Level Of Agreement

Objectives: Early diagnosis and management of rheumatoid arthritis (RA) has improved the outcome of patients. In the last decade, musculoskeletal Ultrasonography (MSUS) had demonstrated its superiority over clinical examination in detecting synovitis in RA. We conducted this present study in order to assess the added value of MSUS in diagnosing early RA. Methods: A cross-sectional study was conducted including one hundred patients diagnosed RA based on the physician opinion and presenting with inflammatory arthralgia or swollen joints for more than 6 weeks and less than 2 years. Patients underwent clinical, laboratory and radiographic examination. MSUS was performed by a radiologist blinded to clinical findings assessing 22 joints of hands. A US ACR/EULAR 2010 score was calculated by replacing the swollen joints of hands by those expressing synovitis in Grey scale US. Agreement between clinical and US ACR/EULAR score was assessed. Results: Among the 2200 joints scanned by US, synovitis was detected in 81% patients, an intra-articular effusion in 36% patients and PD signals in 51% patients. Flexor tenosynovitis were present in 55% patients and extensor tenosynovitis in 59% patients. Synovitis and PD signals were more often detected in wrists. PD mode was found to be correlated with CRP results (r=0,302, p=0,023). The MSUS assessment has demonstrated synovitis on 71% (N=22) patients who were free of swollen joints on clinical examination. Through 13 patients expressing monoarthritis at clinical examination, 69% (N=9) patients were reclassified with oligo or polyarthritis. By adding US data, a further 13 patients accomplished the ACR/EULAR score. A good level of agreement was found between clinical and US ACR/EULAR criteria (k=0,684, p=0,001). Conclusions: MSUS is as an inexpensive and accessible examination tool, which should be considered in patients in the onset of an inflammatory rheumatic disease in order to benefit of the window of opportunity and reach remission.

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SAT0099 Sarcopenia is associated with joint damage in rheumatoid arthritis: a cross-sectional study

10.1136/annrheumdis-2018-eular.6085 ◽

2018 ◽

Author(s):

J.-Z. Lin ◽

Y.-Y. Wang ◽

P.-J. Liu ◽

Y.-Q. Mo ◽

Q.-H. Li ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Joint Damage ◽

Cross Sectional Study ◽

Sectional Study ◽

Cross Sectional

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Serum Level of Endothelial Cell-Specific Molecule -1 (ESM -1) as a New Potential Biomarker for Rheumatoid Arthritis Disease Activity

The Open Rheumatology Journal ◽

10.2174/1874312901812010189 ◽

2018 ◽

Vol 12 (1) ◽

pp. 189-196

Author(s):

Noha Abdelsalam ◽

Ashraf Hussein Mohamed ◽

Sameh Abdellatif ◽

Eslam Eid ◽

Ehsan Mohamed Rizk ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Endothelial Cell ◽

Disease Activity ◽

Severe Disease ◽

Serum Levels ◽

Control Group ◽

Cross Sectional ◽

Das 28 ◽

Rheumatoid Arthritis Disease ◽

Potential Biomarker

Background:Rheumatoid arthritis is a chronic inflammatory autoimmune disease characterized by destruction of the joint cartilage and bone. Endothelial dysfunction (ED) in RA may be related to disease activity. Our objective is to explore serum levels of endothelial cell-specific molecule-1 (ESM-1) as a biomarker for RA disease activity.Methods:A cross-sectional study was carried out and included 83 adult patients with RA, in addition to 20 healthy subjects (age and sex-matched) as a control group. Based on Disease Activity Score in 28 joints (DAS-28), the patient's group was subdivided into four subgroups(remission, mild, moderate and severe disease activity state). The demographic & clinical data, BMI, DAS-28 and Serological assessment [Erythrocyte Sedimentation Rate (ESR), CRP, Rheumatoid Factor (RF) and Anti-Citrullinated Peptide Antibody (ACPA)] were recorded. ESM-1was assayed for all participants.Results:Serum levels of ESM1 were significantly higher in the patient group than the control group (P< 0.0001). ESM-1 serum levels were significantly higher in patients with severe disease activity subgroup compared with patients with remission and mild disease activity subgroups (P< 0.0001). ESM-1 was positively and significantly correlated with DAS-28 score, The Health Assessment Questionnaire Disability Index (HAQ-DI) and modified Larsen score (P= 0.002, 0.0001 & 0.0001 respectively).Conclusion:ESM-1 could be a biomarker for RA disease activity.

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Does serum B lymphocyte chemoattractant protein13 (CXCL13) level correlate with parameters of disease activity and severity in rheumatoid arthritis? Clinical and musculoskeletal ultrasonographic assessment

Egyptian Rheumatology and Rehabilitation ◽

10.1186/s43166-020-00041-2 ◽

2020 ◽

Vol 47 (1) ◽

Author(s):

Eman A. Baraka ◽

Sami A. Egilla ◽

Gamal A. Hamad ◽

Maiada A. Khalil ◽

Shereen H. Ahmed ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Disease Activity ◽

B Lymphocyte ◽

Clinical Laboratory ◽

Serum Levels ◽

Cross Sectional Study ◽

Clinical Disease ◽

Cross Sectional ◽

Recent Onset ◽

Clinical Disease Activity

Abstract Background Rheumatoid arthritis (RA) affects about 1–3% of the population making it one of the most popular autoimmune diseases. Chemokines through switching on their receptors exert a climacteric role in RA pathogenesis. The purpose of this cross-sectional study was to quantify the serum levels of serum B lymphocyte chemoattractant protein13 (CXCL13) chemokine in recent onset RA patients and to correlate its levels with clinical, laboratory, and musculoskeletal ultrasonographic parameters (MSUS) of disease activity and severity. Results The mean serum CXCL13 value showed a significant increase in the RA patients (388.86 ± 283.63 pg/ml) than in the controls (62.94 ± 31.62 pg/ml) (P < 0.001). Highly active RA patients had significantly the highest mean of CXCL13 (mean ± SD 819.13 ± 191.05) compared with the moderately active RA patients (mean ± SD 284.95 ± 137.93) (P < 0.001) and the RA patients with low disease activity (mean ± SD 129.5 ± 21.27) (P < 0.001) and its levels were positively related with clinical disease activity and musculoskeletal ultrasonographic severity parameters. Conclusion Serum CXCL13 is correlated with clinical disease activity and MSUS disease severity that encourages its use for monitoring the activity and severity of synovitis in recent onset RA patients. Future studies to detect the effect of disease activity control by medications on CXCL13 levels and the effect of the CXCL13 antagonist on controlling RA disease activity and severity are recommended.

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CLINICAL CHARACTERISTICS AND DETERMINE THE CONCENTRATIONS OF AUTOBODIES AND CYTOKINES IN PATIENTS WITH RHEUMATOID ARTHRITIS

Journal of Medicine and Pharmacy ◽

10.34071/jmp.2014.6.12 ◽

2015 ◽

pp. 86-92

Author(s):

Van Ai Luu ◽

Tam Vo

Keyword(s):

Rheumatoid Arthritis ◽

Clinical Characteristics ◽

Clinical Manifestations ◽

Joint Damage ◽

Key Words Rheumatoid Arthritis ◽

Cross Sectional Study ◽

Cross Sectional ◽

Tnf Α ◽

Ifn Γ ◽

Positive Results

Background: Rheumatoid arthritis is an autoimmune disease with complex pathophysiological mechanisms, in which cytokines plays an important role. Currently, based on the understanding of the cytokines, the treatment of rheumatoid arthritis with biological agents had changed the course of the disease. Objectives: Study the clinical characteristics and determine the concentrations of autoantibodies (RF, Anti CCP) and cytokines (IL-1α,IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, VEGF, IFN-γ, TNF-α, MCP1, EGF) in patients with rheumatoid arthritis, simultaneously, evaluate the correlation between the cytokines concentrations and levels of autoantibodies in patients with rheumatoid arthritis treated at Cho Ray Hospital.Subjects and Methods: Descriptive cross-sectional study conducted in 76 patients with rheumatoid arthritis treated at Cho Ray Hospital form June 2013 to April 2014.Results: - Clinical and laboratory characteristics: The most common clinical manifestations are arthritis (98.7%), symmetry joint damage (96.1%), morning stiffness over 1 hour (90.8%). Subcutaneous nodule is accounted for 3.9%. The most common joints involved in rheumatoid arthritis are wrist (93.4%), MCP and knee (90.8%), PIP (76.3%) and ankle (67.1%), the least common is the hip (14.5%). The percentage of autoantibodies and cytokines: rheumatoid arthritis patients with positive results of anti – CCP is accounted for 86%. rheumatoid arthritis patients with increased IL-1α concentrations is accounted for 40,8%; increased IL-1β concentration in 48.7%; increased IL-2 concentration in 32,9%; increased IL-4 concentration in 86%; increased IL-6 concentration in 100%; increased IL-8 concentration in 39,5%; increased IL-10 concentration in 81,6%; increased VEGF concentration in 51,3%; increased IFN-γ concentration in 67,1%; increased TNF-α concentration in 61,8%; increased MCP1 concentration in 30,3%; increased EGF concentration in 39,43%. The average concentrations of cytolines in rheumatoid arthritis patients is as followings: IL-1α (57.36 ± 196.43), IL-1β (123.77± 532.51), Il-2 (279.93 ± 945.04), IL-4(279.93 ± 945.04), IL-6(91.35 ± 170.52), IL-8(270.84 ± 445.45), IL-10(134.58 ± 496.14), VEGF(638.87 ± 540.18), IFN-γ(136.43 ± 338.68), TNF-α(106.27 ± 265.57), MCP1(292.34 ± 265.52), EGF(152.62 ± 123.64).RF is correlated with IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-10, IFN-γ, and TNF-α. Anti – CCP is correlated with IL-1α, IL-6. Key words: Rheumatoid arthritis, cytokines

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