scholarly journals Identification of squalamine in the plasma membrane of white blood cells in the sea lamprey,Petromyzon marinus

2007 ◽  
Vol 48 (12) ◽  
pp. 2579-2586 ◽  
Author(s):  
Sang-Seon Yun ◽  
Weiming Li
Keyword(s):  
Plasma Membrane ◽  
Blood Cells ◽  
Petromyzon Marinus ◽  
White Blood Cells ◽  
Sea Lamprey

Haemocytology of the supraneural myeloid body in the sea lamprey during several phases of life cycle

1974 ◽  
Vol 52 (12) ◽  
pp. 1585-1589 ◽  
Author(s):  
J. C. George ◽  
F. W. H. Beamish
Keyword(s):  
Blood Cells ◽  
Petromyzon Marinus ◽  
Cell Types ◽  
Sea Lamprey ◽  
Histochemical Staining ◽  
Fat Cells ◽  
Cell Type ◽  
Adult Tissue ◽  
Spawning Run ◽  
Early Phases

The supraneural myeloid body of the sea lamprey (Petromyzon marinus) was studied in the feeding adult, late spawning run adult, and metamorphosing ammocoete. The fatty nature of the tissue was established by histochemical staining and electron microscopy. The presence of the fat cells and the actively differentiating blood cells evinced its similarity to the bone marrow in higher animals, thereby suggesting a phylogenetic affinity. In the late spawning run lampreys, the tissue was found to be almost empty of blood cells, leaving empty spaces within the stromal skeleton. In the feeding adult tissue, the various blood cell types differentiated from precursor cells have been identified. Megakaryoblasts possibly representing early phases of the cell type were observed only in the transforming (macrophthalmia stage) and adult lampreys. A marked active development of the tissue in the ammocoete was seen only at the fourth stage of the metamorphosing ammocoete immediately before macrophthalmia. At the macrophthalmia stage, the haematopoietic activity in the tissue increased substantially.

In vitro evidence for sodium-dependent pH regulation in sea lamprey (Petromyzon marinus) red blood cells

1992 ◽  
Vol 70 (3) ◽  
pp. 411-416 ◽  
Author(s):  
Bruce L. Tufts
Keyword(s):  
Carbon Dioxide ◽  
Blood Cells ◽  
Ph Regulation ◽  
Petromyzon Marinus ◽  
Sea Lamprey ◽  
Carbon Dioxide Tension ◽  
Factors Influencing ◽  
Rate Of Increase ◽  
Sodium Dependent

Factors influencing the pH of sea lamprey (Petromyzon marinus) erythrocytes were examined in vitro. The absence of extracellular Na+ caused a significant reduction in the erythrocyte pH. In addition, the protonophore 2,4-dinitrophenol was capable of reducing the erythrocyte pH when it was dissolved in dimethyl sulfoxide. In the presence of ouabain, a step increase in the carbon dioxide tension caused a large increase in the intracellular Na+ concentration, but the rate of increase was considerably reduced after the 1st hour. Even in the absence of ouabain, however, the intracellular Na+ concentration in erythrocytes equilibrated with 3% CO2 is much greater than that in erythrocytes equilibrated with 0.2% CO2. Together, these results suggest that Na+-dependent H+ movements, possibly Na+–H+ exchange, may have an important role in erythrocyte pH regulation in P. marinus. Moreover, the mechanism appears to be stimulated by the decrease in extracellular or erythrocyte pH associated with the increase in [Formula: see text]. Extracellular Na+ also has a significant impact on the CO2-transport properties of P. marinus blood. In the absence of extracellular Na+, the intracellular total CO2 concentration was significantly reduced, whereas extracellular total CO2 concentration, [Formula: see text], was significantly increased. Furthermore, in the no-Na+ saline, [Formula: see text] became dependent on the hematocrit; an increase in the number of erythrocytes resulted in an increase in [Formula: see text]. This result suggests that the erythrocyte membrane of P. marinus may be permeable to [Formula: see text].

Blood cell destruction in the opisthonephric kidney of the sea lamprey, Petromyzon marinus L.

1971 ◽  
Vol 49 (6) ◽  
pp. 962-963 ◽  
Author(s):  
John H. Youson
Keyword(s):  
Blood Cell ◽  
Blood Cells ◽  
Petromyzon Marinus ◽  
Sea Lamprey ◽  
Electron Microscopic ◽  
Cell Destruction

Electron microscopic observations reveal that blood cells are engulfed by macrophages within interstitial sinusoids of the opisthonephric kidneys of larval and adult Petromyzon marinus. Therefore the kidneys represent one site where blood cell destruction may occur in this animal.

scholarly journals Modulation of Temoporfin Distribution in Blood by β-Cyclodextrin Nanoshuttles

Pharmaceutics ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1054
Author(s):  
Ilya Yakavets ◽  
Igor Yankovsky ◽  
Tatyana Zorina ◽  
Mikhail Belevtsev ◽  
Lina Bezdetnaya ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Plasma Membrane ◽  
Blood Cells ◽  
Plasma Proteins ◽  
White Blood Cells ◽  
Raji Cells ◽  
Healthy Donors ◽  
Membrane Compartment ◽  
Distribution In Blood ◽  
Spotted Pattern

Photodynamic therapy represents a more targeted and less invasive alternative cancer treatment to traditional modalities. Temoporfin, as with many photosensitizers, is given by injection into a vein, and its subsequent fate is largely determined by the binding to plasma proteins and interaction with endothelial and blood cells. Thus, it is essential to be able to control and to alter the biodistribution of temoporfin in blood. In the present study, we evaluated the effect of co-administration of temoporfin with randomly methylated β-CD (Me-β-CD) on the distribution of temoporfin in the main subpopulations of blood cells of healthy donors using absorbance spectrophotometry and flow cytometry. We showed that cell-bound temoporfin fraction in blood strongly depends on the concentration of Me-β-CD. In fact, the accumulation of temoporfin in white blood cells was more sensitive than that in red blood cells, due to the higher volume of membranous organelles in white blood cells. Finally, we demonstrated that Me-β-CD significantly increases cellular uptake of temoporfin cancer human Burkitt′s lymphoma Raji cells. The presence of Me-β-CD resulted in a spotted pattern of temoporfin distribution in the plasma membrane compartment. Our results clearly demonstrated that β-CDs derivatives provide new options to modulate temoporfin biodistribution in blood.

Glucose transporter expression on the plasma membrane of resting and activated white blood cells

2007 ◽  
Vol 37 (4) ◽  
pp. 282-290 ◽  
Author(s):  
E. Maratou ◽  
G. Dimitriadis ◽  
A. Kollias ◽  
E. Boutati ◽  
V. Lambadiari ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Blood Cells ◽  
Glucose Transporter ◽  
White Blood Cells ◽  
Transporter Expression

Ultrastructural changes in murine lymphoma cells exposed to ultraviolet-A radiation

1991 ◽  
Vol 49 ◽  
pp. 104-105
Author(s):  
Delma P. Thomas ◽  
Dianne E. Godar
Keyword(s):  
Medical Devices ◽  
Blood Cells ◽  
White Blood Cells ◽  
Consumer Products ◽  
Ultrastructural Changes ◽  
Ultraviolet A ◽  
Uv Spectrum ◽  
Lymphoma Cells ◽  
Murine Lymphoma ◽  
Transmission Electron

Ultraviolet radiation (UVR) from all three waveband regions of the UV spectrum, UVA (320-400 nm), UVB (290-320 nm), and UVC (200-290 nm), can be emitted by some medical devices and consumer products. Sunlamps can expose the blood to a considerable amount of UVR, particularly UVA and/or UVB. The percent transmission of each waveband through the epidermis to the dermis, which contains blood, increases in the order of increasing wavelength: UVC (10%) < UVB (20%) < UVA (30%). To investigate the effects of UVR on white blood cells, we chose transmission electron microscopy to examine the ultrastructure changes in L5178Y-R murine lymphoma cells.

The Effect of Red Blood Cells on the ADP-induced Aggregation of Human Platelets in Flow Through Tubes

1990 ◽  
Vol 63 (01) ◽  
pp. 112-121 ◽  
Author(s):  
David N Bell ◽  
Samira Spain ◽  
Harry L Goldsmith
Keyword(s):  
Platelet Aggregation ◽  
Shear Rate ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Platelet Rich Plasma ◽  
Mean Transit Time ◽  
White Blood Cells ◽  
Aggregate Size ◽  
Human Platelets ◽  
Induced Aggregation

SummaryThe effect of red blood cells, rbc, and shear rate on the ADPinduced aggregation of platelets in whole blood, WB, flowing through polyethylene tubing was studied using a previously described technique (1). Effluent WB was collected into 0.5% glutaraldehyde and the red blood cells removed by centrifugation through Percoll. At 23°C the rate of single platelet aggregtion was upt to 9× greater in WB than previously found in platelet-rich plasma (2) at mean tube shear rates Ḡ = 41.9,335, and 1,920 s−1, and at both 0.2 and 1.0 µM ADP. At 0.2 pM ADP, the rate of aggregation was greatest at Ḡ = 41.9 s−1 over the first 1.7 s mean transit time through the flow tube, t, but decreased steadily with time. At Ḡ ≥335 s−1 the rate of aggregation increased between t = 1.7 and 8.6 s; however, aggregate size decreased with increasing shear rate. At 1.0 µM ADP, the initial rate of single platelet aggregation was still highest at Ḡ = 41.9 s1 where large aggregates up to several millimeters in diameter containing rbc formed by t = 43 s. At this ADP concentration, aggregate size was still limited at Ḡ ≥335 s−1 but the rate of single platelet aggregation was markedly greater than at 0.2 pM ADP. By t = 43 s, no single platelets remained and rbc were not incorporated into aggregates. Although aggregate size increased slowly, large aggregates eventually formed. White blood cells were not significantly incorporated into aggregates at any shear rate or ADP concentration. Since the present technique did not induce platelet thromboxane A2 formation or cause cell lysis, these experiments provide evidence for a purely mechanical effect of rbc in augmenting platelet aggregation in WB.

White blood cells levels and PCOS: direct and indirect relationship with insulin resistance, but not with hyperandogenemia

2013 ◽  
Author(s):  
Olga Papalou ◽  
Sarantis Livadas ◽  
Athanasios Karachalios ◽  
Nektarios Benetatos ◽  
George Boutzios ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Blood Cells ◽  
White Blood Cells ◽  
Indirect Relationship
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