Cytokine status in the omentum and peritoneum in metastatic gastric cancer.

2020 ◽  
Vol 38 (15_suppl) ◽  
pp. e16557-e16557
Author(s):  
Elena Yu. Zlatnik ◽  
Oleg I. Kit ◽  
Aleksandr B. Sagakyants ◽  
Ekaterina I. Zolotareva ◽  
Oksana G. Shulgina ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Malignant Tumors ◽  
Metastatic Gastric Cancer ◽  
Greater Omentum ◽  
Specific Content ◽  
Secondary Tumor ◽  
Tnf Α ◽  
Biuret Method ◽  
The Mean

e16557 Background: Gastric cancer (GC) is one of the most common malignant tumors and the second most common cause of cancer deaths in Russia. Many researchers note the ambiguous role of cytokines in the pathogenesis of tumor processes. The purpose of the study was to determine characteristics of the cytokine status in the omentum and peritoneum of patients with metastatic GC. Methods: The main group (Mgr) included 23 patients with T2-4N0-3M1 GC with metastases to the peritoneum and greater omentum, the mean age 58.9±9.7 years. The comparison group (Cgr) included 20 non-cancer patients, the mean age 55.9±9.7 years. Tissues of the omentum (OT) and peritoneum (PT) were obtained from all patients during cytoreductive and diagnostic surgeries. Ex tempore tissues were disintegrated using BD Medimachine (USA) with 0.9% NaCl; the cell suspension was centrifuged, and the supernatant was collected and aliquoted. Levels of cytokines IL-1β, IL-2, IL-6, IL-8, IL-18, TNF-α, IL-10 (Vektor-Best, Russia) and IL-1RA (eBioscience, Austria) were determined by ELISA. The results were expressed as the specific content per 1 g of protein evaluated by the biuret method (pg/mL/g of protein). Statisical analysis of the results was performed with the Statistica 10 program (StatSoft Inc., USA). Significance of differences was evaluated using the Mann-Whitney and Wilkinson tests; differences were considered significant at p < 0.05. Results: In Cgr, levels of IL-6, IL-8, IL-18 and IL-1β in OT were 4.1±1.1, 3.4±0.7, 21.2±5.5 and 10.8±2.1, while in Mgr – 55.3±24.4, 32.5±13.8, 144.9±48.4 and 21.01±5.4 pg/mL/g of protein, i.e. 13.7, 9.5, 6.9 and 1.9 times (p≤0.05) higher than in Cgr, respectively. The specific content of IL-1RA in OT was 12.6 times higher, p≤0.05 (215.7±69.8 vs. 2723.1±730.1), and the IL-10 concentration did not differ significantly. In PT of Cgr, levels of IL-8, IL-18 and IL-6 were 9.8±7.3, 35.2±21.7, 7.5±3.7, and in Mgr – 250.7±21.4, 322.9±16.9, 61.1±27.6 pg/mL/g, i.e. 25.7, 9.2 and 8.2 times (p≤0.05) higher than in Cgr. IL-1RA in PT of Mgr was 24.4 times higher than in Cgr, p≤0.05 (7656.2±3160.3 vs. 357.69±180.86). Conclusions: A certain microenvironment is formed in OT and PT affected by metastases, with specific features in the ratio and concentrations of various cytokines, which undoubtedly contribute to the formation of a secondary tumor.

ROLE OF INTER LEUKIN 10 AND TUMOR NECROSIS FACTOR-ΑLPHA IN PANCREATITIS

2021 ◽  
pp. 14-17
Author(s):  
Mukherjee.J. R ◽  
Mukherjee. B ◽  
Roy. S ◽  
Jana. D ◽  
Bandopadhyay. S ◽  
...  
Keyword(s):  
Tumor Necrosis Factor ◽  
General Surgery ◽  
Tumor Necrosis ◽  
Cell Injury ◽  
Gall Stone ◽  
Tnf Alpha ◽  
Tnf Α ◽  
The Mean ◽  
Necrosis Factor

Background: Pancreatic acinar cell injury triggers the synthesis and release of pro-inammatory cytokines and chemokines. The involvement of several pro-inammatory and anti-inammatory cytokines, such as in interleukin (IL)-1, IL-1β, IL-6, IL-8, IL-10, IL-18, IL-33 and tumor necrosis factor-α is involved in the pathogenesis of pancreatitis. Aim: This study aims to validate the role of activation of TNF-alpha and IL-10 as a biomaker marker in patients with Pancreatitis in Indian subcontinent.Material and methods: 50 Patients of Pancreatitis attending general surgery OPD and admitted to General Surgery department of SSKM Hospital, Kolkata, West Bengal, India were taken. Result: It was found that in alcoholic, the mean TNF - α (mean±s.d.) of the patients was 19.4027 ± 8.3275 pg/ml. In ascites, the mean TNF - α (mean±s.d.) of the patients was 19.9767 ± 2804 pg/ml. In chronic, the mean TNF - α (mean±s.d.) of the patients was 18.8533 ± 8.4674 pg/ml. In gall stone, the mean TNF - α (mean±s.d.) of the patients was 16.3421 ± 9.9499 pg/ml. In osteoarthritis, the mean TNF - α (mean±s.d.) of the patients was 12.4750 ± 8.3085 pg/ml. Distribution of mean TNF - α vs. association was not statistically signicant (p=0.7309).Conclusion: It was found that IL10 was higher in Ascites patients though it was not statistically signicant. TNF alpha was higher in Ascites patients. TNF alpha was higher in normal Pancreatitis.

The Role of Tumor Necrosis Factor-α (TNF-α) Polymorphisms in Gastric Cancer: a Meta-Analysis

2021 ◽  
Author(s):  
Maryam Gholamalizadeh ◽  
Samaneh Mirzaei Dahka ◽  
Hadi Sedigh Ebrahim-Saraie ◽  
Mohammad Esmail Akbari ◽  
Azam Pourtaheri ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Meta Analysis ◽  
Tnf Α ◽  
Factor Α ◽  
Necrosis Factor

The role of the gastrectomy on survival in metastatic gastric cancer patients: A multicenter study of Anatolian Society of Medical Oncology (ASMO).

2015 ◽  
Vol 33 (15_suppl) ◽  
pp. e15052-e15052
Author(s):  
Ozan Yazici ◽  
Nuriye Ozdemir ◽  
Ayse Ocak Duran ◽  
Serkan Menekse ◽  
Mehmet Ali Nahit Sendur ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cancer Patients ◽  
Multicenter Study ◽  
Medical Oncology ◽  
Metastatic Gastric Cancer ◽  
Gastric Cancer Patients

scholarly journals The role of third-line chemotherapy in recurrent or metastatic gastric cancer

Medicine ◽  
2018 ◽  
Vol 97 (39) ◽  
pp. e12588 ◽  
Author(s):  
Yong Won Choi ◽  
Mi Sun Ahn ◽  
Geum Sook Jeong ◽  
Hyun Woo Lee ◽  
Seong Hyun Jeong ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Metastatic Gastric Cancer ◽  
Third Line ◽  
Line Chemotherapy

scholarly journals The role of FGFR2 amplification and expression in patients with advanced or metastatic gastric cancer receiving fluoropyrimidine-based chemotherapy

2018 ◽  
Vol 29 ◽  
pp. viii218
Author(s):  
M.M. Skupinska ◽  
M. Jesiotr ◽  
P. Chrom ◽  
A. Mroz ◽  
S. Cierniak ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Metastatic Gastric Cancer

Intratumoral cisplatin/epinephrine-injectable gel as a palliative treatment for accessible solid tumors: A multicenter pilot study

1998 ◽  
Vol 118 (4) ◽  
pp. 496-503 ◽  
Author(s):  
A. Howard Burris ◽  
Charles L. Vogel ◽  
Dan Castro ◽  
Lopa Mishra ◽  
Maurice Schwarz ◽  
...  
Keyword(s):  
Solid Tumors ◽  
Palliative Treatment ◽  
Malignant Tumors ◽  
Subcutaneous Tissue ◽  
Response Duration ◽  
Median Response ◽  
The Mean ◽  
Histologic Types ◽  
Head Neck

Intratumoral injections of cisplatin/epinephrine-injectable gel were administered weekly for 4 weeks in 45 patients with malignant tumors of various histologic types. Tumors were located on the skin and subcutaneous tissue primarily of the head, neck, and trunk, and on the tongue, oral pharynx, and esophagus. Patients were not candidates for surgery, radiation, or systemic chemotherapy. Each of the treated tumors ( n = 82) was evaluated 2, 4, 8, and 12 weeks after the final injection. The initial dose of cisplatin was 1 mg/cm3 tumor volume, with escalation to 6 mg/cm3 allowed, depending on observed toxicities. The mean cumulative dose per patient for the four treatments ranged from 0.56 to 380 mg cisplatin. No dose-limiting cisplatin-related toxicities, such as nephrotoxicity, neurotoxicity, or ototoxicity, were observed. The overall objective tumor response rate was 50% (41 of 82), with 40% (33 of 82) complete responses and a median response duration of 160 days. Complete responses for adenocarcinoma and squamous cell carcinoma were 58% (21 of 36) and 38% (12 of 32), respectively. These results justified further clinical trials to evaluate the role of local chemotherapy with intratumoral cisplatin/epinephrine-injectable gel in the palliative treatment of patients with selected accessible solid tumors.

Prognostic Role of Primary Tumor Location in Non-Metastatic Gastric Cancer: A Systematic Review and Meta-Analysis of 50 Studies

2017 ◽  
Vol 24 (9) ◽  
pp. 2655-2668 ◽  
Author(s):  
Fausto Petrelli ◽  
Michele Ghidini ◽  
Sandro Barni ◽  
Francesca Steccanella ◽  
Giovanni Sgroi ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Systematic Review ◽  
Primary Tumor ◽  
Meta Analysis ◽  
Metastatic Gastric Cancer ◽  
Tumor Location ◽  
Prognostic Role ◽  
Primary Tumor Location

scholarly journals XAGE-1b Promotes the Proliferation, Invasion and Metastasis of Gastric Cancer

2020 ◽  
Author(s):  
Hui Men ◽  
Zhi-wei Zhang ◽  
Gui-feng Lu ◽  
Shun Tan ◽  
Wen-Hui Hu ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Malignant Tumors ◽  
Survival Curve ◽  
Biological Marker ◽  
Invasion And Metastasis ◽  
Treatment Target ◽  
Kaplan Meier ◽  
Proliferation And Invasion

Abstract Background X antigen family member 1B (XAGE-1b), a member of XAGE subfamily and GAGE family, is upregulated in some malignant tumors and has been associated with the proliferation, invasion and metastasis of tumors. However, the biological roles of XAGE-1b in gastric cancer (GC) still remain unclear. Methods We detected the expression of XAGE-1b in 60 paired fresh tissues of GC patients by real-time RT-PCR. Kaplan-Meier survival curve was explored to analyze 5-year survival time of GC patients. Function experiments were performed to estimate the role of XAGE-1b on the proliferation, invasion and metastasis of GC cells. Informatic analysis was applied to investigate the potential mechanisms. Results XAGE-1b was obviously upregulated in GC tissues. XAGE-1b was correlated significantly with poor prognosis of GC patients. XAGE-1b markedly promoted the proliferation and invasion in GC cell lines in vitro. Knockdown of XAGE-1b promoted the pulmonary metastatic ability in nude mice. Moreover, XAGE-1b was positively or negatively correlated with the expression of CLDN6 and CHGA, which regulated the progression of GC. Conclusions XAGE-1b could act as an oncogene in GC, which provides a potential biological marker or treatment target for GC.

scholarly journals CYTOKINE TISSUE PROFILE IN NON-METASTATIC GASTRIC CANCER

2020 ◽  
Vol 66 (5) ◽  
pp. 524-527
Author(s):  
A. Sagakyants ◽  
Oleg Kit ◽  
Ye. Zlatnik ◽  
Ye. Zolotarev ◽  
O. Shulgina ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Adaptive Immunity ◽  
Tumor Tissue ◽  
Metastatic Gastric Cancer ◽  
Pathological Process ◽  
Special Role ◽  
Functional Elements ◽  
Innate And Adaptive Immunity ◽  
Urgent Task

The development of the tumor process involves the involvement of various functional elements of innate and adaptive immunity. A special role in the reorganization of tissues, changes and evolution of the properties of cancer cells is given to cytokines, the study of the role of which in these processes with various types of neoplasms and, in particular, with nonmetastatic gastric cancer is an urgent task. The aim of the work was to determine the characteristics of the cytokine status in the tissues of patients with non-metastatic gastric cancer. Materials and methods. In the extracts of tumor tissue, perifocal zone, omentum, peritoneum of patients with gastric cancer without metatstases (T3-4aN0-3M0, n = 20, 58.9 ± 9.7 years), the concentration of IL-1P, IL-2, IL -6, IL-8, IL-18, TNF-a, IL-10 and IL-1RA in tumor tissue, the perifocal zone, as well as in the omentum and peritoneum. Results and discussion. In the tumor tissue, an increase in the content of IL-1P, IL-8 and a decrease in IL-2, TNF-a and IL-10 were noted. In the perifocal zone, a decrease in the concentration of IL-1P, IL-6, IL-8 was revealed with an increased content of IL-2, IL-1RA, IL-10, TNF-a and IL-18. Against this background, the omentum and peritoneum compared with the tumor, the concentration of IL-1P, IL-18 and IL-1RA is reduced, against the background of an increase in the concentration of IL-2, IL-6, TNF-a and IL-10. Findings. It was shown that tumor tissue has some features in the cytokine profile compared with unaffected tissues, which can be used to further evaluate the development of the pathological process.

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