1963 ◽  
Vol 43 (3) ◽  
pp. 345-360 ◽  
Author(s):  
Stanley Kushinsky ◽  
Jane (Wu) Tang

ABSTRACT A convenient and mild procedure is described in this paper whereby free and conjugated oestrogens may be extracted from urine. The extracts containing approximately 90 per cent of the oestrogens are devoid of most of the extraneous material and may be reduced in volume to less than 1/15 of that of the urine. The procedure consists of the following steps: (1) a 10 per cent (v/v) solution of a high molecular weight secondary amine (Amberlite LA-2, Rohm and Haas) in ethyl acetate is washed with formic acid and water, (2) the oestrogens in urine (acidified to pH 2 or 3 with H2SO4) are extracted with the LA-2 solution, (3) the oestrogen fraction is back-extracted from the organic solution with dilute aqueous ammonia.


Radiocarbon ◽  
1989 ◽  
Vol 31 (03) ◽  
pp. 231-238 ◽  
Author(s):  
Austin Long ◽  
A T Wilson ◽  
R D Ernst ◽  
B H Gore ◽  
P E Hare

Modern bone contains ca 25% protein material, most of which is collagen. Amino acids separated from collagen isolated from bone are suitable for 14C dating of fossil bone, but attempts to carry out this procedure on bones seriously depleted in protein can yield erroneous 14C dates. Amino-acid analysis of fossil bone gives quantitative information on the degree of preservation of its organic component. Also, the relative abundance of the amino-acid components reveal the degree to which the collagen-like pattern has been altered. Alteration may be caused by addition of extraneous material. A 1mg sample of bone material is sufficient for this preliminary analysis. We have developed a series of acceptance criteria for whether a particular specimen is likely to yield the correct 14C age. 14C dating of fossil bones not seriously depleted in protein is a straightforward procedure and yields reliable dates.


1965 ◽  
Vol 48 (6) ◽  
pp. 1181-1183
Author(s):  
Bob J Miller

Abstract A visual method is presented that enables the analyst to isolate clumps of mold from dark solutions and from colored and irregular bottles and to examine the material microscopically. The solutions to be examined are passed through a glass tube for visual examination, and any observed extraneous material is deposited directly onto a slide. Physical characteristics are not altered, and all visible mold clumps can be accurately counted and measured.


1989 ◽  
Vol 52 (7) ◽  
pp. 503-511 ◽  
Author(s):  
E. C. D. TODD

Data on foodborne disease in Canada in 1984 are compared with those for 1983. A total of 1,181 incidents, comprising 1,016 outbreaks and 165 single cases, caused illnesses in 9,953 persons in 1984. These figures are the highest on record with almost double the number of cases occurring in 1983. Salmonella, Staphylococcus aureus, Clostridium perfringens, and Bacillus cereus were responsible for most of the illnesses. The main Salmonella serovars involved were S. typhimurium, S. enteritidis, and S. heidelberg. There were no incidents of parasitic infections or paralytic shellfish poisonings similar to 1983. Fifty-five incidents (82 cases) of chemical origin were recorded; extraneous matter and rancid compounds were the most frequently implicated. There were two deaths, one from botulism and the other from salmonellosis. Most of the illnesses were associated with meat and poultry (30.6% of incidents and 29.9% of cases). Dairy foods, particularly cheese, bakery products, and marine foods were also major vehicles of foodborne disease. Mishandling of food took place mainly in foodservice establishments (38.8% of incidents), homes (20.6% of incidents), and food processings establishments (5.9% of incidents). Chemicals, such as extraneous material and rancid compounds, were the agents associated with 40.0% of incidents caused by processors' mishandling. On a population basis, incidents were greatest in Ontario, followed by those in Quebec, British Columbia, and Manitoba. Details of several foodborne disease incidents are presented. In addition, seven incidents of waterborne disease were documented in 1984, five more than in 1983. Campylobacter, Salmonella and Yersinia were identified as pathogens associated with drinking water.


Blood ◽  
1958 ◽  
Vol 13 (12) ◽  
pp. 1192-1197 ◽  
Author(s):  
FRANCES L. ESTES ◽  
SHIRLEY SMITH ◽  
JOSEPH H. GAST

Abstract By modification of existing methods, technics have been developed for obtaining polymorphonuclear leukocytes in good yield from intraperitoneal exudates of guinea pigs. The cells were 80-98 per cent viable at the time of harvest and they maintain this viability for at least 24 hours if stored under refrigeration. Good yield, viability and metabolic activity are dependent on careful handling of the cells and the exclusion of extraneous material from the suspending medium. Since the cells are maintained without extraneous material, they are especially suitable for metabolic studies.


1970 ◽  
Vol 48 (4) ◽  
pp. 683-686 ◽  
Author(s):  
J. F. Wardell ◽  
J. H. Hart

The response of sapwood of swamp white oak (Quercus bicolor Willd.) to mechanical injury was studied over a 28-day period. In cells within 0.5 cm of a wound stimulus, starch grains disappeared and cells were unable to reduce the vital stain, triphenyl tetrazolium chloride (TTC), 8 to 10 days after mechanical injury. Extraneous material and tannins began to accumulate 10 to 12 days after mechanical injury. Nuclei began to disintegrate 12 to 14 days after mechanical injury, but some persisted throughout the period of investigation.With increasing distance from the cambium, starch grains disappeared from ray parenchyma. Cells in the annual rings of sapwood adjacent to the heartwood were unable to reduce TTC. Nuclei were present in all annual rings of sapwood but were lacking in heartwood. Tannins appeared at the heartwood boundary and accumulated in the heartwood.With respect to the criteria used, this investigation indicates that formation of discoloration in sapwood and conversion of sapwood to heartwood appear similar.


Holzforschung ◽  
2007 ◽  
Vol 61 (5) ◽  
pp. 492-498 ◽  
Author(s):  
Biljana Bujanovic ◽  
Sally A. Ralph ◽  
Richard S. Reiner ◽  
Rajai H. Atalla

Abstract Commercial softwood kraft pulp with kappa number 30.5 (KP30.5) was delignified with polyoxometalates (POM, Na5(+2)[SiV1(-0.1)MoW10(+0.1)O40]), and POM-treated kraft pulp of kappa number 23.6 was obtained (KPPOM,23.6). Residual lignin from pulps was isolated by mild acid hydrolysis and characterized by analytical and spectral methods to gain insight into lignin reactions taking place during the initial delignification phase. Lignin from POM-delignified pulp was isolated in lower yield. Comparative analysis of residual lignins (RL-KP30.5, RL-KPPOM,23.6) showed that POM leads to products enriched in carbonyl/carboxyl groups and carbohydrates. POM lignins have a lower molecular mass and a lower content of phenolic hydroxyl and methoxyl groups. Based on these results and FTIR spectra, we suggest that aromatic ring cleavage and quinone formation occur during POM delignification. The degree of lignin-cellulose association increases after POM delignification. Lignin-cellulose association was found to be partially unstable under mild alkaline conditions, as residual lignin isolated after alkaline extraction of KPPOM,23.6 pulp (RL-KPPOM/NaOH) exhibited lower glucose content, higher Klason lignin content, and less extraneous material.


2007 ◽  
Vol 71 (6) ◽  
pp. 520-533
Author(s):  
Nicola Haralambous

This article challenges the unconditional faith seemingly placed on juries by the judiciary. Despite the prohibition on investigation into jury deliberations, a number of important cases have come to light which invite questions on the extent to which we can be sure that juries follow judicial directions. This article explores the recent case law in which juries may have relied upon extraneous material or other external influences and, finally, briefly compares such impropriety with the use of jurors with specialised knowledge.


1974 ◽  
Vol 20 (11) ◽  
pp. 1523-1527 ◽  
Author(s):  
R. L. Anacker ◽  
R. K. Gerloff ◽  
L. A. Thomas ◽  
R. E. Mann ◽  
W. R. Brown ◽  
...  

Rocky Mountain spotted fever rickettsiae, cultivated either in yolk sacs of embryonated chicken eggs or in mouse L cells, were purified by centrifugation through a linear sucrose density gradient in a zonal rotor. Under the conditions of the experiments the rickettsiae banded at a density of about 1.21. Intact organisms with what appeared to be large translucent capsules were found in this band. The degree of purity was high because yolk sac contamination was not detected in rickettsial suspensions derived from embryonated eggs, and little extraneous material was observed by electron microscopy in suspensions obtained from either host. About 4 × 108 rickettsial particles were recovered from each gram of yolk sac and 2 × 1010 particles from each gram of L cells, a 50-fold difference. Each milligram dry weight of purified rickettsiae contained about 1 × 1010 particles.


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