Introduction to the Behavioral Biology of Laboratory Animals

Anesthesia for laboratory animals is a matter of biomedical concern and one of the most present dilemmas in the current bioethical debate. The use of anesthetic agents in experimental surgery aims at analgesia and restraining the animal, in order to achieve a reasonable degree of muscle relaxation and to produce sufficient analgesia. This practice requires the use of protocols for the administration of safe and efficient doses. Eight New Zealand rabbits were submitted to laparotomies demonstrating the surgical technique discipline of the local medical course. For pre-anesthetic medication, acepromazine 1 mg.kg-1 associated with ketamine 15 mg.kg-1 was used subcutaneously. Anesthesia was maintained with isoflurane and oxygen under a laryngeal mask in a Mapleson D anesthesia system and under spontaneous breathing. Hydration was performed with 10 ml.kg-1 saline every hour. A thermal mattress was used. Precordial stethoscope, pulse oximetry and clinical parameters were used for monitoring. For euthanasia, ketamine 10 mg.kg-1 associated with potassium chloride 19.1% 1 ml.kg-1 was used intravenously. The average weight of the rabbits was 2721.25 ± 275.01 grams and the duration of the anesthetic procedure was 120 ± 87 minutes. Discussion. In long-term anesthesia, such as laparotomies, the use of pre-anesthetic medication and then anesthetic induction by the combination of agents is recommended. However, anesthetic management requires monitoring to prevent insufficient or excessive doses from occurring.

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Efficacy of praziquantel (Cesocide® injection) in treatment of cestode infections in domestic and laboratory animals

The Korean Journal of Parasitology ◽

10.3347/kjp.1988.26.2.121 ◽

1988 ◽

Vol 26 (2) ◽

pp. 121 ◽

Cited By ~ 6

Author(s):

Kee Seon Eom ◽

Seung Ho Kim ◽

Han Jong Rim

Keyword(s):

Laboratory Animals

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B-cell and T-cell values in peripheral blood in Polish mixed-breed rabbits with addition of blood of meet breeds

Polish Journal of Veterinary Sciences ◽

10.2478/pjvs-2014-0060 ◽

2014 ◽

Vol 17 (3) ◽

pp. 421-426 ◽

Cited By ~ 1

Author(s):

B. Tokarz-Deptuła ◽

P. Niedźwiedzka-Rystwej ◽

B. Hukowska-Szematowicz ◽

M. Adamiak ◽

A. Trzeciak-Ryczek ◽

...

Keyword(s):

T Cells ◽

B Cells ◽

B Cell ◽

Cd8 T Cells ◽

Peripheral Blood ◽

Laboratory Animals ◽

Immunological Parameters ◽

Four Seasons ◽

Mixed Breed ◽

The Impact

Abstract In Poland, rabbit is a highly valued animal, due to dietetic and flavour values of its meat, but above all, rabbits tend to be commonly used laboratory animals. The aim of the study was developing standards for counts of B-cells with CD19+ receptor, T-cells with CD5+ receptor, and their subpopulations, namely T-cells with CD4+, CD8+ and CD25+ receptor in the peripheral blood of mixed-breed Polish rabbits with addition of blood of meet breeds, including the assessment of the impact of four seasons of the year and animal sex on the values of the immunological parameters determined. The results showed that the counts of B- and T-cells and their subpopulations in peripheral blood remain within the following ranges: for CD19+ B-cells: 1.05 - 3.05%, for CD5+ T-cells: 34.00 - 43.07%, CD4+ T-cells: 23.52 - 33.23%, CD8+ T-cells: 12.55 - 17.30%, whereas for CD25+ T-cells: 0.72 - 2.81%. As it comes to the season of the year, it was observed that it principally affects the values of CD25+ T-cells, while in the case of rabbit sex, more changes were found in females.

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Automation for improved physiological research in microgravity using laboratory animals

10.2514/6.1996-4326 ◽

1996 ◽

Author(s):

David Conrad ◽

John Emond ◽

Louis Stodieck

Keyword(s):

Laboratory Animals ◽

Physiological Research

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Calculations used for assessment of rapid maxillary expansion

ORL ro ◽

10.26416/orl.30.1.2016.525 ◽

2016 ◽

Vol 1 (1) ◽

pp. 24-26

Author(s):

Ruxandra Bartok ◽

Bogdan Dimitriu Dimitriu ◽

Constantin Vârlan ◽

Radu Stanciu ◽

Georgiana Moldoveanu ◽

...

Keyword(s):

Tensile Strength ◽

Elasticity Modulus ◽

Rapid Maxillary Expansion ◽

Laboratory Animals ◽

Maxillary Expansion ◽

Literature Reference ◽

Bone Changes ◽

Upper Jaw ◽

Materials Used

Rapid maxillary expansion is defined as the release of medio-palatine suture using an orthopedic forces. The role of this procedure is to expand the upper jaw in order to achieve the broadening of the upper arch. This study was initiated to quantify the effects of disjunction and post- treatment bone changes, after an adequate contention which lasted for three weeks. This study is carried on laboratory animals (common breed rabbit) to determine tensile strength and the elasticity modulus of biological materials used in orthdodontics. The results of the study are consistent with those reported in the literature reference.

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PRECLINICAL STUDY OF THE VETERINARY DRUGS TOXICITY

Vestnik of the Russian agricultural science ◽

10.30850/vrsn/2019/5/61-64 ◽

1970 ◽

pp. 61-64

Author(s):

E. K. Rakhmatullin ◽

O. D. Sklyarov

Keyword(s):

Lethal Dose ◽

Clinical Manifestations ◽

Preclinical Study ◽

Veterinary Drugs ◽

Toxic Effects ◽

Laboratory Animals ◽

Therapeutic Effects ◽

Significant Information ◽

Important Indicator ◽

Organ Systems

Preclinical study of the drugs toxicity was analysed it allows predicting the safety of veterinary drugs in laboratory animals. The fundamental normative instruments in the field of preclinical study of drugs for veterinary medicine and animal husbandry are Order of the Ministry of Agriculture of the Russian Federation dated 06.03.2018 N 101 and GOST 33044-2014 Principles of Good Laboratory Practice. An important indicator of the preclinical study of the veterinary drugs is the determination (calculation) of median lethal dose value (lethal dose for half of the animals tested) or concentration (LD50 or LC50). Existing methods for determining this indicator make it possible at the initial study stage to determine the degree and class the drug of toxicity. Studying the symptoms of intoxication in the analysis of pharmacological substances one obtains significant information about the nature of the action of the future drug. The clinical manifestations of intoxication with damage to various organ systems are presented. As criteria for assessing the toxic effects of veterinary drugs it is recommended to determine LD50, cumulation coefficient, latitude index of therapeutic effects, dose level of toxic effects in the experiment which allows predicting the nature and degree of toxic effects of the drug even at the stage of preclinical veterinary drugs study.

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