Anesthesia for rabbits submitted to experimental surgeries: Report of a series of eight anesthesias

2020 ◽  
pp. 151-158
Author(s):  
Rafael Antonio Caldart Bedin ◽  
Maisa Schultz ◽  
Antonio Bedin
Keyword(s):  
Muscle Relaxation ◽  
Anesthetic Management ◽  
Laboratory Animals ◽  
Average Weight ◽  
Anesthetic Induction ◽  
Anesthetic Agents ◽  
Reasonable Degree ◽  
Bioethical Debate ◽  
Precordial Stethoscope

Anesthesia for laboratory animals is a matter of biomedical concern and one of the most present dilemmas in the current bioethical debate. The use of anesthetic agents in experimental surgery aims at analgesia and restraining the animal, in order to achieve a reasonable degree of muscle relaxation and to produce sufficient analgesia. This practice requires the use of protocols for the administration of safe and efficient doses. Eight New Zealand rabbits were submitted to laparotomies demonstrating the surgical technique discipline of the local medical course. For pre-anesthetic medication, acepromazine 1 mg.kg-1 associated with ketamine 15 mg.kg-1 was used subcutaneously. Anesthesia was maintained with isoflurane and oxygen under a laryngeal mask in a Mapleson D anesthesia system and under spontaneous breathing. Hydration was performed with 10 ml.kg-1 saline every hour. A thermal mattress was used. Precordial stethoscope, pulse oximetry and clinical parameters were used for monitoring. For euthanasia, ketamine 10 mg.kg-1 associated with potassium chloride 19.1% 1 ml.kg-1 was used intravenously. The average weight of the rabbits was 2721.25 ± 275.01 grams and the duration of the anesthetic procedure was 120 ± 87 minutes. Discussion. In long-term anesthesia, such as laparotomies, the use of pre-anesthetic medication and then anesthetic induction by the combination of agents is recommended. However, anesthetic management requires monitoring to prevent insufficient or excessive doses from occurring.

scholarly journals General anesthesia with remimazolam in a patient with mitochondrial encephalomyopathy: a case report

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Yuji Suzuki ◽  
Matsuyuki Doi ◽  
Yoshiki Nakajima
Keyword(s):  
Lactic Acidosis ◽  
General Anesthesia ◽  
Mitochondrial Disease ◽  
Muscle Relaxation ◽  
Anesthetic Management ◽  
Perioperative Period ◽  
Mitochondrial Encephalomyopathy ◽  
Anesthesia Induction ◽  
General Anesthetic ◽  
Anesthetic Agents

Abstract Background Systemic anesthetic management of patients with mitochondrial disease requires careful preoperative preparation to administer adequate anesthesia and address potential disease-related complications. The appropriate general anesthetic agents to use in these patients remain controversial. Case presentation A 54-year-old woman (height, 145 cm; weight, 43 kg) diagnosed with mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes underwent elective cochlear implantation. Infusions of intravenous remimazolam and remifentanil guided by patient state index monitoring were used for anesthesia induction and maintenance. Neither lactic acidosis nor prolonged muscle relaxation occurred in the perioperative period. At the end of surgery, flumazenil was administered to antagonize sedation, which rapidly resulted in consciousness. Conclusions Remimazolam administration and reversal with flumazenil were successfully used for general anesthesia in a patient with mitochondrial disease.

scholarly journals Anesthetic experimental device for small animal

2007 ◽  
Vol 22 (3) ◽  
pp. 229-233 ◽  
Author(s):  
Luiz Alfredo de Magalhães Vivas ◽  
Nelson Jamel ◽  
Ricardo Antonio Refinetti ◽  
Luis Felipe da Silva ◽  
Lígia Villela Rodrigues ◽  
...  
Keyword(s):  
Low Cost ◽  
Anesthetic Management ◽  
Small Animal ◽  
Laboratory Animals ◽  
New Materials ◽  
New Techniques ◽  
Teaching And Research ◽  
Anesthetic Agents ◽  
Unidirectional Valve ◽  
Anesthesia Procedure

PURPOSE: The difficulty to anesthetize small laboratory animals with vaporizer prompted us to go in search of new materials, and create new techniques. The improved equipment of anesthesia we looked for should be low cost, practical, versatile, and its management serve ethical, teaching, and research purposes. METHODS: The new components of the equipment were: the vaporizer, the unidirectional valve, the glass cylinder filled with water, the flow guidance y-shape tube, the flow regulators, the mask modifications, and another free airway for emergency occurrence. A test was done with 30 Wistar rats, Rattus norvegicus albinus, divided into three groups with 10 rats for each one. Groups 1, 2 and 3 were anesthetized with Ether, Halothane and Sevoflurane respectively, using the new gadget. The anesthetic induction time, the breathing rhythm alteration during an anesthesia pre-established time (10 minutes), and the recovery time were observed. RESULTS: The equipment enabled an easy handling, and fulfilled a larger safeness and stability during the induction and anesthetic management. The test showed it was possible to make use of several anesthetic agents. CONCLUSION: The device is effective, and turns the anesthesia procedure into a very easy practice with low-cost. It should be recommended for experimental surgery, teaching and research.

Intraoperative Anesthesiology Management and Patient Outcomes for Surgical Revascularization for Moyamoya Disease: A Review and Clinical Experience

2019 ◽  
Vol 80 (03) ◽  
pp. 143-148
Author(s):  
George Williams ◽  
William Jones ◽  
Rabail Chaudhry ◽  
Chunyan Cai ◽  
Greesha Pednekar ◽  
...  
Keyword(s):  
Moyamoya Disease ◽  
Medical Center ◽  
Anesthetic Management ◽  
Hospital Length ◽  
Hospital Length Of Stay ◽  
Neurosurgical Procedures ◽  
Surgical Revascularization ◽  
Anesthetic Agents ◽  
Intraoperative Management

Background Moyamoya disease (MMD) is a rare cerebrovascular condition, often presenting as a headache or stroke in adults. Anesthetic management of this illness may challenge providers because it can affect the long-term neurologic outcome and hospital length of stay (LOS) in patients with MMD. Materials and Methods A literature search was conducted to assess etiology and epidemiology, as well as existing reports of intraoperative management of MMD. Due to sparse findings, the search was expanded to include studies of the use of intraoperative anesthetic agents during other neurosurgical procedures. We also retrospectively reviewed all MMD cases from January 1, 2009, to December 31, 2015, at Memorial Hermann Hospital-Texas Medical Center, where intraoperative management involved craniotomy and surgical revascularization. Data were collected primarily on the use of several anesthetic agents. The LOS and any adverse events were also recorded for each case. The data were divided into two equivalent case cohorts: (1) January 1, 2009, to February 18, 2013, and (2) February 19, 2013, to December 31, 2015. Results Remifentanil use notably increased between the first and second time periods while fentanyl use decreased. Desflurane usage also demonstrated an observed increase when our two cohorts were compared. Additionally, there was a decrease in the mean LOS between the first and second periods of 3.9 and 3.3 days, respectively. Conclusion Increasing use of remifentanil in MMD cases could be attributed to its ability to provide more stable hemodynamics during induction, maintenance, and emergence of anesthesia when compared with fentanyl. Lower systolic pressures, diastolic pressures, and heart rates were reported in patients receiving remifentanil over fentanyl.

Memory impairment induced by amphetamine derivatives in laboratory animals and in humans: a review

2012 ◽  
Vol 3 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Jordi Camarasa ◽  
Teresa Rodrigo ◽  
David Pubill ◽  
Elena Escubedo
Keyword(s):  
Illicit Drugs ◽  
Molecular Mechanisms ◽  
Memory Performance ◽  
Laboratory Animals ◽  
Club Drugs ◽  
Synthetic Drugs ◽  
History Of ◽  
Amphetamine Derivatives ◽  
Considerable Body

AbstractThe 20th century brought with it the so-called club drugs (the most notorious being amphetamine derivatives), which are used by young adults at all-night dance parties. Methamphet­amine and 3,4-methylenedioxymethamphetamine (MDMA or ecstasy) are synthetic drugs with stimulant and psychoactive properties that belong to the amphetamine family. Here, we have reviewed the literature about the cognitive impairment induced by these two amphetamine derivatives and the preclinical and clinical outcomes. Although there is controversial evidence about the effect of methamphetamine and MDMA on learning and memory in laboratory animals, results from published papers demonstrate that amphetamines cause long-term impairment of cognitive functions. A large number of pharmacological receptors have been studied and screened as targets of amphetamine-induced cognitive dysfunction, and extensive research efforts have been invested to provide evidence about the molecular mechanisms behind these cognitive deficits. In humans, there is a considerable body of evidence indicating that methamphetamine and MDMA seriously disrupt memory and learning processes. Although an association between the impairments of memory performance and a history of recreational amphetamine ingestion has also been corroborated, a number of methodological difficulties continue to hamper research in this field, the most important being the concomitant use of other illicit drugs.

Ethical Implications of Electroconvulsive Therapy: A Review

2021 ◽  
pp. EHPP-D-21-00002
Author(s):  
David González-Pando ◽  
Ana González-Menéndez ◽  
Víctor Aparicio-Basauri ◽  
César Luís Sanz de la Garza ◽  
José Esteban Torracchi-Carrasco ◽  
...  
Keyword(s):  
Lived Experience ◽  
Electroconvulsive Therapy ◽  
Medical Literature ◽  
Well Being ◽  
Conclusive Evidence ◽  
Healthcare Provision ◽  
Ethical Implications ◽  
Safe Technique ◽  
Bioethical Debate

This article examines in depth whether electroconvulsive therapy (ECT) satisfies bioethics’ four healthcare provision principles: nonmaleficence, beneficence, justice, and autonomy. Treating patients ethically implies protecting them from damages, working to ensure their well-being, and respecting their decisions once they have been correctly informed. Although most of the medical literature states that ECT is an effective and safe technique, there is no conclusive evidence of long-term effectiveness. The frequent and sometimes persistent side effects such as amnesia that imply a profound disturb of lived experience, or the difficulties regarding the informed consent process, allow us to conclude that the bioethical debate about ECT is not currently closed, and the information provided to patients needs to be revised.

Trauma: The Burn Patient

2018 ◽  
pp. 81-86
Author(s):  
Ethan Kim ◽  
Ruchir Gupta
Keyword(s):  
Carbon Monoxide ◽  
Fluid Therapy ◽  
Muscle Relaxation ◽  
Anesthetic Management ◽  
Preoperative Evaluation ◽  
Initial Assessment ◽  
Area Burned ◽  
Intraoperative Management ◽  
Parkland Formula ◽  
Burn Patient

In this chapter the essential aspects of anesthetic management of the burn patient are discussed. Subtopics include use of the Parkland formula for calculating fluid requirements, airway considerations, and carbon monoxide toxicity. The differences between first-, second-, and third-degree burns are also discussed. The chapter is divided into preoperative, intraoperative, and postoperative sections with important subtopics related to the main topic in each section. Issues discussed that are related to preoperative evaluation include initial assessment, calculating percentage of body area burned, and airway evaluation. Topics related to intraoperative management include muscle relaxation, monitoring, and fluid therapy. Postoperative issues involve extubation criteria and treatment to induce muscle relaxation.

Time Required to Achieve Homogeneity of Test Substances Added to Dog Feed

1991 ◽  
Vol 74 (5) ◽  
pp. 857-861
Author(s):  
Philip P Sapienza ◽  
George J Ikeda ◽  
Patricia I Warr ◽  
Richard H Albert
Keyword(s):  
Nicotinic Acid ◽  
Animal Feed ◽  
Mixing Time ◽  
Critical Factor ◽  
Laboratory Animals ◽  
Time Period ◽  
Time Test ◽  
Time Required ◽  
Carrier Animal

Abstract The homogeneity of test substances in a carrier (animal feed) is a critical factor In conducting long-term feeding studies in laboratory animals. A method for determining the adequate amount of mixing to achieve homogeneity by a mixer of the type described has been determined when 2 distinctly different compounds are added to ground dog feed. Nicotinic acid and butylated hydroxyanlsole at a concentration of 1% were separately mixed with the dog feed for 15,30,45,60, and 120 min to determine optimum mixing time. Test portions were taken from 4 different sampling sites at each time period and analyzed in duplicate for the added substance. Four batches were prepared and the results were aggregated. Very little interbatch variability was observed. The variance of the average values from the 4 sampling sites at each time period was calculated and used as a simple, crude, but effective numerical quantity to monitor the approach to homogeneity of the mixture.

Implementation of the Three Rs in Biomedical Research —Has the Turn of the Century Turned the Tide?

2009 ◽  
Vol 37 (2) ◽  
pp. 197-207 ◽  
Author(s):  
Shoko Obora ◽  
Tsutomu Kurosawa
Keyword(s):  
Biomedical Research ◽  
Nature Medicine ◽  
Turn Of The Century ◽  
Laboratory Animals ◽  
The Public ◽  
Journal Editors ◽  
Time Period ◽  
Large Fluctuations ◽  
The Three Rs

There has been increasing pressure from the public against animal experimentation for testing and research purposes. The Three Rs ( replacement, reduction, and refinement) principle is thought to be a key foundation concept in optimising the welfare of animals used in experiments. This retrospective study attempts to investigate the transition of the Three Rs in biomedical research through a review of articles published in Nature Medicine. We categorised all of the articles published in Nature Medicine from 1998 to 2003, on the basis of the pain and distress of the animals used in the experiments featured in the analysed article. We found there were no large fluctuations in the distribution of these categories over this time period. We also examined each article for the presence of a statement relating to the humane use of laboratory animals, and found that the number of articles which included such a statement dramatically increased in 2002. Over the years studied, there was a decreasing trend in the total number of animal types used for the experiments in the articles. Our results suggest that: a) more encouragement by journal editors might improve the attitude of scientists in terms of animal welfare; and b) the progress of replacement appears to be a more long-term effort in the field of biomedical research.

scholarly journals Hypertension and Experimental Stroke Therapies

2013 ◽  
Vol 33 (8) ◽  
pp. 1141-1147 ◽  
Author(s):  
Victoria E O'Collins ◽  
Geoffrey A Donnan ◽  
Malcolm R Macleod ◽  
David W Howells
Keyword(s):  
Basic Science ◽  
Meta Analysis ◽  
Experimental Stroke ◽  
Science Data ◽  
Anesthetic Agents ◽  
Superior Efficacy ◽  
Lower Treatment ◽  
Tissue Plasminogen ◽  
The Impact

Hypertension is an established target for long-term stroke prevention but procedures for management of hypertension in acute stroke are less certain. Here, we analyze basic science data to examine the impact of hypertension on candidate stroke therapies and of anti-hypertensive treatments on stroke outcome. Methods: Data were pooled from 3,288 acute ischemic stroke experiments (47,899 animals) testing the effect of therapies on infarct size (published 1978-2010). Data were combined using meta-analysis and meta-regression, partitioned on the basis of hypertension, stroke model, and therapy. Results: Hypertensive animals were used in 10% of experiments testing 502 therapies. Hypertension was associated with lower treatment efficacy, especially in larger infarcts. Overall, anti-hypertensives did not provide greater benefit than other drugs, although benefits were evident in hypertensive animals even when given after stroke onset. Fifty-eight therapies were tested in both normotensive and hypertensive animals: some demonstrated superior efficacy in hypertensive animals (hypothermia) while others worked better in normotensive animals (tissue plasminogen activator, anesthetic agents). Discussion: Hypertension has a significant effect on the efficacy of candidate stroke drugs: standard basic science testing may overestimate the efficacy which could be reasonably expected from certain therapies and for hypertensive patients with large or temporary occlusions.

Intraoperative Monitoring of Central Neurophysiology

2017 ◽  
Author(s):  
Alan D. Legatt ◽  
Marc R. Nuwer ◽  
Ronald G. Emerson
Keyword(s):  
Intraoperative Monitoring ◽  
Corticospinal Tract ◽  
Motor Evoked Potentials ◽  
Anesthetic Management ◽  
Depth Of Anesthesia ◽  
Central Nervous System Function ◽  
Anatomy And Physiology ◽  
Anesthetic Agents ◽  
Nervous System Function ◽  
Signal Changes

This chapter covers neurophysiological intraoperative monitoring (NIOM). It describes the relevant neurophysiological signals, their anatomical sources, the techniques used to record them, the manner in which they are assessed, and possible causes of intraoperative signal changes. Techniques used include electroencephalography (EEG), electromyography, and auditory, somatosensory, and motor evoked potentials. Some of these techniques can be used to localize and identify areas of cerebral cortex or the corticospinal tract. Recording of the electromyogram generated by reflex activity can be used to evaluate central nervous system function in some circumstances. EEG can be used to assess depth of anesthesia. Signals can be affected by anesthesia, and the chapter discusses various anesthetic agents, their effects on signals, and considerations for anesthetic management during NIOM. Personnel performing NIOM must be knowledgeable about the anatomy and physiology underlying the signals, the technology used to record them, and the factors (including anesthesia) that can affect them.

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