Bone Morphogenetic Protein-2 Enhances Osteoclast Formation Mediated by Interleukin-1α through Upregulation of Osteoclast Differentiation Factor and Cyclooxygenase-2

1999 ◽  
Vol 259 (1) ◽  
pp. 97-102 ◽  
Author(s):  
Masanori Koide ◽  
Yoshiyuki Murase ◽  
Kenji Yamato ◽  
Toshihide Noguchi ◽  
Nobuo Okahashi ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Osteoclast Differentiation ◽  
Osteoclast Formation ◽  
Cyclooxygenase 2 ◽  
Bone Morphogenetic Protein 2 ◽  
Differentiation Factor ◽  
Morphogenetic Protein ◽  
Osteoclast Differentiation Factor ◽  
Interleukin 1Α

Bone morphogenetic protein 2 controls steroid-induced osteonecrosis of the femoral head via directly inhibiting interleukin-34 expression

2021 ◽  
Author(s):  
Meng Wang ◽  
Hong Sung Min ◽  
Haojie Shan ◽  
Yiwei Lin ◽  
Wenyang Xia ◽  
...  
Keyword(s):  
Femoral Head ◽  
Bone Morphogenetic Protein ◽  
Inflammatory Responses ◽  
Gene Knockout ◽  
Osteoclast Differentiation ◽  
Bone Morphogenetic Protein 2 ◽  
Qrt Pcr ◽  
Morphogenetic Protein ◽  
Local Expression

Increased inflammatory responses is one of the major characteristics of osteonecrosis of the femoral head (ONFH). We aimed to investigate the function of bone morphogenetic protein 2 (BMP-2)/interleukin (IL)-34 axis in the inflammatory responses of ONFH. The systemic and local expression of BMPs in ONFH patients were detected by qRT-PCR and ELISA. In vitro osteoclast differentiation and ONFH mouse models, induced by 20 mg/kg methylprednisolone through intramuscular injection, were established using wild type and BMP-2-/- mice to explore the regulatory role of BMP-2 in pro-inflammatory responses and bone defects of ONFH. IL-34 expression and function were examined in vitro and in vivo through qRT-PCR, TRAP staining, and gene knockout. The systemic and local expression of BMPs were elevated in ONFH patients. BMP-2 reduced the production of pro-inflammatory cytokines and inhibited the differentiation of osteoclasts. Mechanistically, BMP-2 inhibited osteoclasts formation through suppressing IL-34 expression, and then promoted bone repair and alleviated ONFH. In conclusion, our study reveals that BMP-2 inhibits inflammatory responses and osteoclast formation through down-regulating IL-34.

Simvastatin inhibits osteoclast differentiation induced by bone morphogenetic protein-2 and RANKL through regulating MAPK, AKT and Src signaling

2010 ◽  
Vol 162 (1-3) ◽  
pp. 99-108 ◽  
Author(s):  
Misuzu Yamashita ◽  
Fumio Otsuka ◽  
Tomoyuki Mukai ◽  
Ryutaro Yamanaka ◽  
Hiroyuki Otani ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Osteoclast Differentiation ◽  
Bone Morphogenetic Protein 2 ◽  
Src Signaling ◽  
Morphogenetic Protein

scholarly journals Cyclooxygenase-2 inhibitor reduces simvastatin-induced bone morphogenetic protein-2 and bone formation in vivo

2007 ◽  
Vol 42 (3) ◽  
pp. 267-273 ◽  
Author(s):  
J. D. Bradley ◽  
D. G. Cleverly ◽  
A. M. Burns ◽  
N. B. Helm ◽  
M. J. Schmid ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Morphogenetic Protein ◽  
Cyclooxygenase 2 ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein

scholarly journals The Antiosteoporosis Effects of Yishen Bugu Ye Based on Its Regulation on the Differentiation of Osteoblast and Osteoclast

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Yangyang Li ◽  
Yongfeng Zhang ◽  
Weiqi Meng ◽  
Yutong Li ◽  
Tao Huang ◽  
...  
Keyword(s):  
Trabecular Bone ◽  
Bone Morphogenetic Protein ◽  
Type I Collagen ◽  
Osteoclast Differentiation ◽  
Bone Morphogenetic Protein 2 ◽  
Inflammatory Factors ◽  
Tartrate Resistant Acid Phosphatase ◽  
Type I ◽  
Serum Concentrations ◽  
Morphogenetic Protein

Yishen Bugu Ye (YSBGY), a traditional Chinese medicine comprising 12 types of medicinal herbs, is often prescribed in China to increase bone strength. In this study, the antiosteoporotic effects of YSBGY were investigated in C57BL/6 mice afflicted with dexamethasone- (Dex-) induced osteoporosis (OP). The results showed that YSBGY reduced the interstitial edema in the liver and kidney of mice with Dex-induced OP. It also increased the number of trabecular bone elements and chondrocytes in the femur, promoted cortical bone thickness and trabecular bone density, and modulated the OP-related indexes in the femur and tibia of OP mice. It also increased the serum concentrations of type I collagen, osteocalcin, osteopontin, bone morphogenetic protein-2, bone morphogenetic protein receptor type 2, C-terminal telopeptide of type I collagen, and runt-related transcription factor-2 and reduced those of tartrate-resistant acid phosphatase 5 and nuclear factor of activated T cells in these mice, suggesting that it improved osteoblast differentiation and suppressed osteoclast differentiation. The anti-inflammatory effect of YSBGY was confirmed by the increase in the serum concentrations of interleukin- (IL-) 33 and the decrease in concentrations of IL-1, IL-7, and tumor necrosis factor-α in OP mice. Furthermore, YSBGY enhanced the serum concentrations of superoxide dismutase and catalase in these mice, indicating that it also exerted antioxidative effects. This is the first study to confirm the antiosteoporotic effects of YSBGY in mice with Dex-induced OP, and it showed that these effects may be related to the YSBGY-induced modulation of the osteoblast/osteoclast balance and serum concentrations of inflammatory factors. These results provide experimental evidence supporting the use of YSBGY for supporting bone formation in the clinical setting.

Mechanical strain inhibits expression of osteoclast differentiation factor by murine stromal cells

2000 ◽  
Vol 278 (6) ◽  
pp. C1126-C1132 ◽  
Author(s):  
Janet Rubin ◽  
Tamara Murphy ◽  
Mark S. Nanes ◽  
Xian Fan
Keyword(s):  
Stromal Cells ◽  
Mechanical Strain ◽  
Osteoclast Differentiation ◽  
Osteoclast Formation ◽  
Thymidine Uptake ◽  
Differentiation Factor ◽  
Osteoclast Differentiation Factor ◽  
Membrane Bound ◽  
Macrophage Colony Stimulating Factor ◽  
Macrophage Colony

Normal dynamic loading prevents bone resorption; however, the means whereby biophysical factors reduce osteoclast activity are not understood. We show here that mechanical strain (2% at 10 cycles per minute) applied to murine marrow cultures reduced 1,25(OH)2D3-stimulated osteoclast formation by 50%. This was preceded by decreased expression of osteoclast differentiation factor (ODF/TRANCE). RT-PCR for ODF/TRANCE revealed that ODF/TRANCE mRNA in strained cultures was 59 ± 3% of that seen in control cultures. No significant effects on total cell count, thymidine uptake, or alkaline phosphatase activity were induced by strain. To isolate the cell targeted by strain, primary stromal cells were cultured from marrow. Mechanical strain also reduced mRNA for ODF/TRANCE to 60% that of control in these cells. In contrast, mRNA for membrane-bound macrophage colony-stimulating factor was not significantly affected. Soluble ODF (∼2 ng/ml) was able to reverse the effect of strain, returning osteoclast numbers to control. Because osteoclast formation is dependent upon ODF/TRANCE expression, strain-induced reductions in this factor may contribute to the accompanying reduction in osteoclastogenesis.

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