Thebric à braclocus consists of two paralogous genes encoding BTB/POZ domain proteins and acts as a homeotic and morphogenetic regulator of imaginal development inDrosophila

Development ◽  
2002 ◽  
Vol 129 (10) ◽  
pp. 2419-2433 ◽  
Author(s):  
Jean-Louis Couderc ◽  
Dorothea Godt ◽  
Susan Zollman ◽  
Jiong Chen ◽  
Michelle Li ◽  
...  
Keyword(s):  
Tissue Distribution ◽  
Nuclear Protein ◽  
Drosophila Species ◽  
Sexually Dimorphic ◽  
Conserved Domains ◽  
Gene Dose ◽  
Highly Sensitive ◽  
Genes Encoding ◽  
Redundant Functions ◽  
Related Morphology

The bric à brac (bab) locus acts as a homeotic and morphogenetic regulator in the development of ovaries, appendages and the abdomen. It consists of two structurally and functionally related genes, bab1 and bab2, each of which encodes a single nuclear protein. Bab1 and Bab2 have two conserved domains in common, a BTB/POZ domain and a Psq domain, a motif that characterizes a subfamily of BTB/POZ domain proteins in Drosophila. The tissue distribution of Bab1 and Bab2 overlaps, with Bab1 being expressed in a subpattern of Bab2. Analysis of a series of mutations indicates that the two bab genes have synergistic, distinct and redundant functions during imaginal development. Interestingly, several reproduction-related traits that are sexually dimorphic or show diversity among Drosophila species are highly sensitive to changes in the bab gene dose, suggesting that alterations in bab activity may contribute to evolutionary modification of sex-related morphology.

scholarly journals Differential Gene Expression in Response to Hydrogen Peroxide and the Putative PerR Regulon of Synechocystis sp. Strain PCC 6803

2004 ◽  
Vol 186 (11) ◽  
pp. 3331-3345 ◽  
Author(s):  
Hong Li ◽  
Abhay K. Singh ◽  
Lauren M. McIntyre ◽  
Louis A. Sherman
Keyword(s):  
Chlorophyll Biosynthesis ◽  
Short Term ◽  
Histidine Kinases ◽  
Highly Sensitive ◽  
Number Of Genes ◽  
Genes Encoding ◽  
Differential Gene ◽  
Regulatory Properties ◽  
Knockout Mutation ◽  
Pcc 6803

ABSTRACT We utilized a full genome cDNA microarray to identify the genes that comprise the peroxide stimulon in the cyanobacterium Synechocystis sp. strain PCC 6803. We determined that a gene (slr1738) encoding a protein similar to PerR in Bacillus subtilis was induced by peroxide. We constructed a PerR knockout strain and used it to help identify components of the PerR regulon, and we found that the regulatory properties were consistent with the hypothesis that PerR functions as a repressor. This effort was guided by finding putative PerR boxes in positions upstream of specific genes and by careful statistical analysis. PerR and sll1621 (ahpC), which codes for a peroxiredoxin, share a divergent promoter that is regulated by PerR. We found that isiA, encoding a Chl protein that is induced under low-iron conditions, was strongly induced by a short-term peroxide stress. Other genes that were strongly induced by peroxide included sigD, sigB, and genes encoding peroxiredoxins and Dsb-like proteins that have not been studied yet in this strain. A gene (slr1894) that encoded a protein similar to MrgA in B. subtilis was upregulated by peroxide, and a strain containing an mrgA knockout mutation was highly sensitive to peroxide. A number of genes were downregulated, including key genes in the chlorophyll biosynthesis pathway and numerous regulatory genes, including those encoding histidine kinases. We used PerR mutants and a thioredoxin mutant (TrxA1) to study differential expression in response to peroxide and determined that neither PerR nor TrxA1 is essential for the peroxide protective response.

scholarly journals Mapping of genes encoding coagulation factors VII and X to the distal portion of the 13q34 by gene dose study in a patient with r(13)

1989 ◽  
Vol 34 (3) ◽  
pp. 247-250 ◽  
Author(s):  
Ryozo Kasai ◽  
Kouji Narahara ◽  
Hiroshi Namba ◽  
Kazushiro Tsuji ◽  
Tsunenori Matsubara ◽  
...  
Keyword(s):  
Coagulation Factors ◽  
Distal Portion ◽  
Gene Dose ◽  
Genes Encoding ◽  
Dose Study

scholarly journals Redundant functions of the SLC5A transporters Rumpel Kumpel and Bumpel in ensheathing glial cells

2021 ◽  
Author(s):  
Kerem Yildirim ◽  
Bente Winkler ◽  
Nicole Pogodalla ◽  
Stefanie Mackensen ◽  
Marie Baldenius ◽  
...  
Keyword(s):  
Energy Source ◽  
Glial Cells ◽  
Ovarian Development ◽  
Sugar Metabolism ◽  
Nutrient Sensing ◽  
Genes Encoding ◽  
Neuronal Processing ◽  
Solute Carrier ◽  
And Behavior ◽  
Redundant Functions

Neuronal processing is energy demanding, and relies on sugar metabolism as an energy source. To provide a constant metabolite supply neurons and glial cells express many glucose and lactate transporters of the solute carrier (SLC) 5A family. Here we dissect the partially redundant functions of three highly related glia specific Drosophila genes encoding SLC5A proteins, Rumpel, Bumpel and Kumpel. While knockdown of rumpel causes several behavioral phenotypes, they are less prominent in rumpel mutants. bumpel and kumpel mutants are viable and fertile, lacking discernible phenotypes. However, in bumpel kumpel double mutants and to an even greater extent in rumpel bumpel kumpel triple mutants oogenesis is disrupted at the onset of the vitollegenic phase. This indicates at least partially redundant functions between these genes. Rescue experiments exploring this effect indicate that oogenesis can be affected by CNS glial cells. Moreover, expression of heterologous mammalian SLC5A transporter proteins, with known transport properties, suggest that Bumpel and/or Kumpel transport glucose or lactate. Overall, our results imply a redundancy in SLC5A nutrient sensing functions in Drosophila glial cells, affecting ovarian development and behavior.

Role of the Vps9-domain protein RgfA inDictyosteliumchemotaxis and development

2013 ◽  
Vol 59 (1) ◽  
pp. 22-27
Author(s):  
Jeffrey A. Hadwiger
Keyword(s):  
Cell Growth ◽  
Heterologous Expression ◽  
Rab Proteins ◽  
Nucleotide Exchange ◽  
Surface Receptors ◽  
Guanine Nucleotide Exchange ◽  
Nucleotide Exchange Factors ◽  
Genes Encoding ◽  
Redundant Functions ◽  
External Signals

Proteins with a Vps9 domain function as guanine nucleotide exchange factors for Rab proteins and can mediate the uptake of cell surface receptors or other molecules through endocytosis. However, genes encoding these proteins have not been previously studied in cells with robust chemotactic capabilities. Several genes encoding Vps9 domains were identified in the genome of Dictyostelium discoideum, and one of the genes, designated as rgfA (DDB_G0272038), was examined for functions in cell growth, development, and chemotaxis. The rgfA gene was expressed during vegetative growth and throughout development, but disruption of this gene resulted in no major alterations in cell growth, macropinocytosis, developmental morphology, or chemotactic movement. However, heterologous expression of RgfA resulted in a delay of developmental morphogenesis and impaired chemotaxis of cells to folate but did not affect macropinocytosis. These results suggest that RgfA might share redundant functions with other Dictyostelium Vps9-domain proteins and that heterologous expression of this protein can alter processes that depend on the reception of external signals.

scholarly journals Purification of mouse MEP-1, a nuclear protein which binds to the metal regulatory elements of genes encoding metallothionein

1993 ◽  
Vol 21 (7) ◽  
pp. 1549-1554 ◽  
Author(s):  
Simon Labbé ◽  
Lucie Larouche ◽  
Donald Mailhot ◽  
Carl Séguin
Keyword(s):  
Nuclear Protein ◽  
Regulatory Elements ◽  
Genes Encoding

Tissue distribution of protease resistant prion protein in variant Creutzfeldt-Jakob disease using a highly sensitive immunoblotting assay

The Lancet ◽  
2001 ◽  
Vol 358 (9277) ◽  
pp. 171-180 ◽  
Author(s):  
JDF Wadsworth ◽  
S Joiner ◽  
AF Hill ◽  
TA Campbell ◽  
M Desbruslais ◽  
...  
Keyword(s):  
Tissue Distribution ◽  
Prion Protein ◽  
Highly Sensitive ◽  
Immunoblotting Assay ◽  
Jakob Disease

scholarly journals A Stable Cell Line Expressing Clustered AChR: A Novel Cell-Based Assay for Anti-AChR Antibody Detection in Myasthenia Gravis

2021 ◽  
Vol 12 ◽  
Author(s):  
Yu Cai ◽  
Lu Han ◽  
Desheng Zhu ◽  
Jing Peng ◽  
Jianping Li ◽  
...  
Keyword(s):  
Myasthenia Gravis ◽  
Cell Line ◽  
Stable Expression ◽  
Antibody Detection ◽  
Stable Cell Line ◽  
Healthy Individuals ◽  
Achr Antibody ◽  
Highly Sensitive ◽  
Genes Encoding ◽  
Radioimmunoprecipitation Assay

Cell-based assays (CBAs) and radioimmunoprecipitation assay (RIPA) are the most sensitive methods for identifying anti-acetylcholine receptor (AChR) antibody in myasthenia gravis (MG). But CBAs are limited in clinical practice by transient transfection. We established a stable cell line (KL525) expressing clustered AChR by infecting HEK 293T cells with dual lentiviral vectors expressing the genes encoding the human AChR α1, β1, δ, ϵ and the clustering protein rapsyn. We verified the stable expression of human clustered AChR by immunofluorescence, immunoblotting, and real-time PCR. Fluorescence-activated cell sorting (FACS) was used to detect anti-AChR antibodies in 103 MG patients and 58 healthy individuals. The positive results of MG patients reported by the KL525 was 80.6% (83/103), 29.1% higher than the 51.4% (53/103) of RIPA. 58 healthy individuals tested by both the KL525 CBA and RIPA were all negative. In summary, the stable expression of clustered AChR in our cell line makes it highly sensitive and advantageous for broad clinical application in CBAs.

scholarly journals Prevalence of Adhesion Genes fnbA & fnbB of Staphylococcus aureus Isolated from Dental Patients Attending Dentistry Department of Tehsil Headquarter Hospital Pasrur, Pakistan

2020 ◽  
Vol 1 (4) ◽  
Author(s):  
Muhammad Danish Mehmood ◽  
Huma Anwar Ul-Haq ◽  
Khushbu Farva ◽  
Zuhaib Farooq ◽  
Gul Muhammad Shaikh ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Specific Primers ◽  
Dental Patients ◽  
Gram Staining ◽  
Microbiological Methods ◽  
Highly Sensitive ◽  
Genes Encoding ◽  
Pure Cultures ◽  
Polymerase Chain ◽  
Adhesive Molecules

Introduction: The present study was intended to isolate, characterize and investigate the prevalence of virulence genes encoding fibronectin (fnbA and fnbB) adhesive molecules of S. aureus from dental patients attending outpatient department of THQ Hospital Pasur. Methodology: A total of 100 oral samples were collected from dental patients, pure cultures was segregated and identified through conventional microbiological methods to evaluate the prevalence of S. aureus. Isolates were further characterized by using specific primers for genotype fnbA and fnbB. Results: Results of the study declared that 68 samples were positive (68%) for staphylococcus aureus on the basis of growth on selective media and appearance of typical colonies supported by gram staining. These gram positive staphylococci were positive (86.7%) in coagulase and catalase testing. The results of polymerase chain reaction (PCR) revealed that 47 (69.1%) and 23 (33.8%) isolates showed amplification with type specific primer 23Sr RNA and NUC gene respectively. Furthermore, fnbA and fnbB type specific genes of S. aureus did not show any amplification in PCR reaction. Conclusion: Irrespectively the data in the present study showed the prevalence of S. aureus is significantly high in males and of age group of 20-40 years but no positive result was found for prevalence of fnbA and fnbB genes. All the S. aureus isolates were highly sensitive to vancomycin, linezolid and clindamycin.

scholarly journals Comparative Analysis of Differentially Expressed Genes in Shewanella oneidensis MR-1 following Exposure to UVC, UVB, and UVA Radiation

2005 ◽  
Vol 187 (10) ◽  
pp. 3556-3564 ◽  
Author(s):  
Xiaoyun Qiu ◽  
George W. Sundin ◽  
Liyou Wu ◽  
Jizhong Zhou ◽  
James M. Tiedje
Keyword(s):  
Uv Radiation ◽  
Short Wavelength ◽  
Cellular Response ◽  
Lethal Factor ◽  
Shewanella Oneidensis ◽  
Uva Irradiation ◽  
Detoxification Mechanism ◽  
Highly Sensitive ◽  
Genes Encoding ◽  
Heavy Metal Efflux

ABSTRACT We previously reported that Shewanella oneidensis MR-1 is highly sensitive to UVC (254 nm), UVB (290 to 320 nm), and UVA (320 to 400 nm). Here we delineated the cellular response of MR-1 to UV radiation damage by analyzing the transcriptional profile during a 1-h recovering period after UVC, UVB, and UVA exposure at a dose that yields about a 20% survival rate. Although the SOS response was observed with all three treatments, the induction was more robust in response to short-wavelength UV radiation (UVB and UVC). Similarly, more prophage-related genes were induced by short-wavelength UV radiation. MR-1 showed an active detoxification mechanism in response to UVA, which included the induction of antioxidant enzymes and iron-sequestering proteins to scavenge reactive oxygen species. In addition, a great number of genes encoding multidrug and heavy metal efflux pumps were induced following UVA irradiation. Our data suggested that activation of prophages appears the major lethal factor in MR-1 following UVC or UVB irradiation, whereas oxidative damage contributes greatly to the high UVA sensitivity in MR-1.

scholarly journals Highly Sensitive and Specific Detection of Staphylococcal Enterotoxins SEA, SEG, SEH, and SEI by Immunoassay

Toxins ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 130
Author(s):  
Cécile Féraudet Tarisse ◽  
Céline Goulard-Huet ◽  
Yacine Nia ◽  
Karine Devilliers ◽  
Dominique Marcé ◽  
...  
Keyword(s):  
Food Poisoning ◽  
Cross Reactivity ◽  
Threat Detection ◽  
Enzyme Immunoassays ◽  
Staphylococcal Enterotoxins ◽  
Strain Characterization ◽  
Highly Sensitive ◽  
Genes Encoding ◽  
Contaminated Food ◽  
First Time

Staphylococcal food poisoning (SFP) is one of the most common foodborne diseases worldwide, resulting from the ingestion of staphylococcal enterotoxins (SEs), primarily SE type A (SEA), which is produced in food by enterotoxigenic strains of staphylococci, mainly S. aureus. Since newly identified SEs have been shown to have emetic properties and the genes encoding them have been found in food involved in poisoning outbreaks, it is necessary to have reliable tools to prove the presence of the toxins themselves, to clarify the role played by these non-classical SEs, and to precisely document SFP outbreaks. We have produced and characterized monoclonal antibodies directed specifically against SE type G, H or I (SEG, SEH or SEI respectively) or SEA. With these antibodies, we have developed, for each of these four targets, highly sensitive, specific, and reliable 3-h sandwich enzyme immunoassays that we evaluated for their suitability for SE detection in different matrices (bacterial cultures of S. aureus, contaminated food, human samples) for different purposes (strain characterization, food safety, biological threat detection, diagnosis). We also initiated and described for the first time the development of monoplex and quintuplex (SEA, SE type B (SEB), SEG, SEH, and SEI) lateral flow immunoassays for these new staphylococcal enterotoxins. The detection limits in buffer were under 10 pg/mL (0.4 pM) by enzyme immunoassays and at least 300 pg/mL (11 pM) by immunochromatography for all target toxins with no cross-reactivity observed. Spiking studies and/or bacterial supernatant analysis demonstrated the applicability of the developed methods, which could become reliable detection tools for the routine investigation of SEG, SEH, and SEI.

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