The differentiation of mouse gonads in vitro: a light and electron microscopical study

Development ◽  
1986 ◽  
Vol 97 (1) ◽  
pp. 189-199
Author(s):  
Sarah Mackay ◽  
Robert A. Smith
Keyword(s):  
Electron Microscopy ◽  
Culture Medium ◽  
Light And Electron Microscopy ◽  
Calf Serum ◽  
Microscopical Study ◽  
Electron Microscopical Study ◽  
The Other ◽  
Foetal Calf Serum ◽  
Electron Microscopical

Indifferent urogenital complexes were excised from mouse foetuses assessed by developmental criteria as day 10·5 or 11. After 4 or 6–7 days in culture, complexes were fixed and examined by light and electron microscopy. The effect of culturing sexed complexes in mixed sex groups was investigated. The effect of the presence or absence of foetal calf serum in the culture medium was considered. No evidence of inhibition of one sex by the other was found. Ovaries developed further in cultures than testes.

Biomaterial-induced alterations of human neutrophils under fluid shear stress: scanning electron microscopical study in vitro

Biomaterials ◽  
1996 ◽  
Vol 17 (14) ◽  
pp. 1359-1367 ◽  
Author(s):  
J. Tomczok ◽  
W. Sliwa-tomczok ◽  
C.L. Klein ◽  
T.G. Van Kooten ◽  
C.J. Kirkpatrick
Keyword(s):  
Shear Stress ◽  
Fluid Shear Stress ◽  
Microscopical Study ◽  
Electron Microscopical Study ◽  
Human Neutrophils ◽  
Fluid Shear ◽  
Electron Microscopical ◽  
Scanning Electron

scholarly journals ELECTRON MICROSCOPICAL STUDY OF SKELETAL MUSCLE DURING ISOTONIC (AFTERLOAD) AND ISOMETRIC CONTRACTION

1956 ◽  
Vol 2 (2) ◽  
pp. 201-211 ◽  
Author(s):  
G. G. Knappeis ◽  
F. Carlsen
Keyword(s):  
Electron Microscopy ◽  
Skeletal Muscle ◽  
Isometric Contraction ◽  
Sarcomere Length ◽  
Microscopical Study ◽  
Electron Microscopical Study ◽  
Semitendinosus Muscle ◽  
Electron Microscopical ◽  
Contraction Band

Bundles of the curarized semitendinosus muscle of the frog were fixed during isotonic (afterload) and isometric contraction and the length of the A and I bands investigated by electron microscopy. The sarcomere length, during afterload contraction initiated at 25 per cent stretch, varied depending on the afterload applied between 3.0 and 1.2 µ, i.e. the shortening amounted to 5 to 50 per cent. The shortening involved both the A and I bands. Between a sarcomere length of 3.0 to 1.7 µ (shortening 5 to 35 per cent) the A bands remained practically constant at about 1.5 µ (6 to 8 per cent shortening); the length of the I bands decreased from 1.4 to 0.3 µ (80 per cent shortening). Below a sarcomere length of 1.7 to 1.2 µ the A bands shortened from 1.5 to 1.0 µ (from 6 to 8 to 25 per cent). At sarcomere lengths 1.6 to 1.2 µ the I band was replaced by a contraction band. During isometric contraction the A bands shortened by about 8 to 10 per cent; the I bands were correspondingly elongated.

The Effect of Certain Fixatives on Particles of a Globular Protein: An Electron-Microscopical Study

1964 ◽  
Vol s3-105 (70) ◽  
pp. 227-230
Author(s):  
K. DEUTSCH ◽  
E. FISCHER ◽  
W. KRAUSE
Keyword(s):  
Electron Microscopy ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Potassium Permanganate ◽  
Bovine Serum ◽  
Osmium Tetroxide ◽  
Microscopical Study ◽  
Globular Protein ◽  
Electron Microscopical Study ◽  
Electron Microscopical

Particles (regarded as molecules) of bovine serum albumin have been studied by electron microscopy both in the untreated state and after fixation with osmium tetroxide, formaldehyde, and potassium permanganate. All three fixatives, especially potassium permanganate, increased the density of the particles considerably.

The bovine alveolar macrophage. 1. Isolation, in vitro cultivation, ultrastructure, and phagocytosis

1973 ◽  
Vol 19 (10) ◽  
pp. 1207-1210 ◽  
Author(s):  
M. L. Fox
Keyword(s):  
Electron Microscopy ◽  
Staphylococcus Aureus ◽  
Alveolar Macrophage ◽  
Calf Serum ◽  
In Vitro Cultivation ◽  
Foetal Calf Serum ◽  
Isolated Cells ◽  
Large Numbers ◽  
Bovine Alveolar Macrophage

The bovine alveolar macrophage (BAM) was isolated in large numbers from lung washings and could be cultivated in vitro up to 63 days in medium 199, 20% foetal calf serum (FCS). Freshly isolated cells examined by electron microscopy (EM) were similar to those of the mouse. Cultured BAM prepared for EM were larger than fresh cells and contained discrete packets of homogenous material, possibly lipid. The BAM phagocytosed Staphylococcus aureus (FDA209P, type 42D) but the percentage of cells phagocytosing was reduced when FCS was not present, when cells were infected for 30 min compared with 45 min and when 2 × 107 CFU/ml compared with 2 × 108 CFU/ml were used to infect the cells.

Development of an in vitro Model for the Study of the Response of Equine Tendon Fibroblasts to Injury and Medication

1997 ◽  
Vol 10 (01) ◽  
pp. 6-11 ◽  
Author(s):  
R. F. Rosenbusch ◽  
L. C. Booth ◽  
L. A. Dahlgren
Keyword(s):  
Electron Microscopy ◽  
Extracellular Matrix ◽  
Light Microscopy ◽  
Light And Electron Microscopy ◽  
Tendon Healing ◽  
Matrix Proteins ◽  
Isolated Cells ◽  
Superficial Digital Flexor Tendon ◽  
Vitro Model

SummaryEquine tendon fibroblasts were isolated from explants of superficial digital flexor tendon, subcultured and maintained in monolayers. The cells were characterized by light microscopy, electron microscopy and radiolabel studies for proteoglycan production. Two predominant cell morphologies were identified. The cells dedifferentiated toward a more spindle shape with repeated subcultures. Equine tendon fibroblasts were successfully cryopreserved and subsequently subcultured. The ability to produce proteoglycan was preserved.The isolated cells were identified as fibroblasts, based on their characteristic shape by light microscopy and ultrastructure and the active production of extracellular matrix proteins. Abundant rough endoplasmic reticulum and the production of extracellular matrix products demonstrated active protein production and export. Proteoglycans were measurable via liquid scintillation counting in both the cell-associated fraction and free in the supernatant. This model is currently being utilized to study the effects of polysulfated glycosaminoglycan on tendon healing. Future uses include studying the effects of other pharmaceuticals, such as hyaluronic acid, on tendon healing.A model was developed for in vitro investigations into tendon healing. Fibroblasts were isolated from equine superficial digital flexor tendons and maintained in monolayer culture. The tenocytes were characterized via light and electron microscopy. Proteoglycan production was measured, using radio-label techniques. The fibroblasts were cryopreserved and subsequently subcultured. The cells maintained their capacity for proteoglycan production, following repeated subculturing and cryopreservation.

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