A Histochemical Study of Dehydrogenase Activity in the Pectoralis Major Muscle of the Pigeon and certain other Vertebrate Skeletal Muscles

1958 ◽  
Vol s3-99 (48) ◽  
pp. 469-473
Author(s):  
J. C. GEORGE ◽  
K. S. SCARIA
Keyword(s):  
Dehydrogenase Activity ◽  
Skeletal Muscles ◽  
Pectoralis Major Muscle ◽  
Breast Muscle ◽  
Muscle Fibres ◽  
Triphenyl Tetrazolium Chloride ◽  
Tetrazolium Chloride ◽  
Unique System ◽  
Oxidative Processes ◽  
The Individual

Certain dehydrogenases in the breast muscle of the pigeon and fowl and the leg muscle of the fowl and frog were studied histochemically by the use of 2:3:5: triphenyl tetrazolium chloride. The dehydrogenase activity was found to have a relationship with the colour and the mitochondrial content of the individual muscle fibres. In the pigeon breast muscle, however, the broad white fibres did not show the presence of any of the enzymes studied. It is therefore concluded that these fibres in the pigeon breast muscle are a unique system in which none of the oxidative processes concerned takes place; they cannot be considered as analogous to the white fibres of the other muscles studied.

Valine, malic, and pyruvic dehydrogenase tests in the differentiation of Bacteroides

1975 ◽  
Vol 21 (9) ◽  
pp. 1369-1371
Author(s):  
Noel R. Funderburk ◽  
A. S. Kester
Keyword(s):  
Amino Acids ◽  
Organic Acids ◽  
Dehydrogenase Activity ◽  
Rapid Method ◽  
Pyruvic Acid ◽  
Triphenyl Tetrazolium Chloride ◽  
Hydrogen Acceptor ◽  
Tetrazolium Chloride

Fifteen isolates of Bacteroides were tested for their ability to dehydrogenate a variety of amino acids and organic acids. A simple and rapid method was developed for detecting dehydrogenase activity using 2,3,5-triphenyl tetrazolium chloride as a hydrogen acceptor and indicator. The results indicate that the tests for valine, malic, and pyruvic acid dehydrogenases have value in differentiating organisms in this genus.

scholarly journals The foetal development of lactate dehydrogenase isoenzymes, glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase from human striated muscle

1969 ◽  
Vol 111 (2) ◽  
pp. 219-224 ◽  
Author(s):  
Jørgen Clausen ◽  
Robert Hustrulid
Keyword(s):  
Lactate Dehydrogenase ◽  
Dehydrogenase Activity ◽  
Skeletal Muscles ◽  
Muscle Fibres ◽  
Relative Distribution ◽  
Foetal Development ◽  
Lactate Dehydrogenase Activity ◽  
Phosphogluconate Dehydrogenase ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Lactate Dehydrogenase Isoenzymes

1. Human foetal skeletal muscles involved in support and in periodic contractility were studied for their content of total extractable lactate dehydrogenase, glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase activities as well as for the relative distribution of lactate dehydrogenase isoenzymes. 2. During foetal development a linear steady increase in total lactate dehydrogenase activity as well as a linear decrease in the H/M sub-unit ratio of the isoenzymes was found. 3. No significant changes were found in the activities of the enzymes of the hexose monophosphate shunt (C-6 oxidation). 4. The changes found suggest a steady increased synthesis of lactate dehydrogenase M-sub-units in human skeletal muscles during foetal development. 5. The weekly changes in the total lactate dehydrogenase activity and in lactate dehydrogenase isoenzymes are lower in muscles involved in support than in those involved in periodic contractility. 6. These findings, together with the literature available, are consistent with the morphological fact that foetal development of skeletal muscles mostly concerns the white muscle fibres and not the red muscle fibres.

scholarly journals Potential Fertilization Capacity of Two Grapevine Varieties: Effects on Agricultural Production in Designation of Origin Areas in the Northwestern Iberian Peninsula

Agronomy ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 961
Author(s):  
J. Antonio Cortiñas Rodríguez ◽  
María Fernández-González ◽  
Estefanía González-Fernández ◽  
Rosa A. Vázquez-Ruiz ◽  
F. Javier Rodríguez-Rajo ◽  
...  
Keyword(s):  
Airborne Pollen ◽  
Germination Rate ◽  
Pollen Viability ◽  
Weather Conditions ◽  
Triphenyl Tetrazolium Chloride ◽  
Tetrazolium Chloride ◽  
The Mean ◽  
Study Sites ◽  
Fertilization Capacity

In the present study, we analyzed the main parameters related with the potential fertilization ability of two grapevine varieties, Godello and Mencía, during the years 2017 and 2018. The research was carried out in two vineyards of the Galician winegrowing Designation of Origin areas of Ribeiro and Ribeira Sacra. Ten vines of each variety were selected for bunch and flower counting, pollen calculations, pollen viability studies by means of aceto-carmine (AC) stain and 2, 3, 5-triphenyl tetrazolium chloride (TTC) methods, and the determination of their germination rate. In all vineyards the 50% fruitset was reached, except for Godello in Cenlle during 2017. The mean coulure value was higher for Godello (40.5%) than for Mencía (31%). Analyzing the pollen production per plant and airborne pollen levels, we observed important discordances between them, which can be due to the influence of weather conditions and be related with self-pollination processes. We found important differences on pollen viability depending on the applied method and variety, with higher values for the AC method than the TTC for both varieties in all study plots, and higher values for Mencía variety than Godello. Regarding germination rates, we observed a marked reduction in 2017 with respect to 2018, in all study sites and for both varieties. The analyzed parameters were useful to explain the different productive abilities of Godello and Mencía varieties in the two studied bioclimatic regions of Ribeiro and Ribeira Sacra.

Oxygen uptake and tetrazolium reduction during skin cycle of the mouse

1965 ◽  
Vol 209 (1) ◽  
pp. 231-235 ◽  
Author(s):  
Ira M. Dushoff ◽  
John Payne ◽  
Falls B. Hershey ◽  
Robert C. Donaldson
Keyword(s):  
Oxygen Consumption ◽  
Mouse Skin ◽  
Growth Cycle ◽  
Triphenyl Tetrazolium Chloride ◽  
Intact Skin ◽  
Tetrazolium Chloride ◽  
Quantitative Measurements ◽  
Close Relationship ◽  
Coefficient Of Regression ◽  
Tetrazolium Reduction

Quantitative measurements of oxygen consumption and triphenyl tetrazolium chloride reduction by intact skin were made during various stages of the skin cycle of the mouse. Values are highest during early anagen, when growth is most rapid: Qo2 is 2.47 ± 0.10 (mean ± se) and TTC is 537 ± 24.6. In late anagen the activities decrease 40–50%: Qo2 is 1.24 ± 0.10 and TTC is 315 ± 20.8. There is a close relationship between tetrazolium reduction and oxygen consumption during all phases of the growth cycle of mouse skin. The coefficient of regression of TTC on Qo2 is 163.2 ( P < .001).

scholarly journals Sarcoglycan complex in masseter and sternocleidomastoid muscles of baboons: an immunohistochemical study

2015 ◽  
Vol 59 (2) ◽  
Author(s):  
G. Cutroneo ◽  
A. Centofanti ◽  
F. Speciale ◽  
G. Rizzo ◽  
A. Favaloro ◽  
...  
Keyword(s):  
Skeletal Muscles ◽  
Immunohistochemical Study ◽  
Epigenetic Modification ◽  
Expression Profiles ◽  
Muscle Fibres ◽  
Social Ranking ◽  
Healthy Humans ◽  
The Social ◽  
Almost All ◽  
Contractile Forces

<p>The sarcoglycan complex consists of a group of single-pass transmembrane glycoproteins that are essential to maintain the integrity of muscle membranes. Any mutation in each sarcoglycan gene causes a series of recessive autosomal dystrophin-positive muscular dystrophies. Negative fibres for sarcoglycans have never been found in healthy humans and animals. In this study, we have investigated whether the social ranking has an influence on the expression of sarcoglycans in the skeletal muscles of healthy baboons. Biopsies of masseter and sternocleidomastoid muscles were processed for confocal immunohistochemical detection of sarcoglycans. Our findings showed that baboons from different social rankings exhibited different sarcoglycan expression profiles. While in dominant baboons almost all muscles were stained for sarcoglycans, only 55% of muscle fibres showed a significant staining. This different expression pattern is likely to be due to the living conditions of these primates. Sarcoglycans which play a key role in muscle activity by controlling contractile forces may influence the phenotype of muscle fibres, thus determining an adaptation to functional conditions. We hypothesize that this intraspecies variation reflects an epigenetic modification of the muscular protein network that allows baboons to adapt progressively to a different social status.</p>

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