scholarly journals Authentication of saffron (Crocus sativus L.) using 1H nuclear magnetic resonance (NMR) spectroscopy

Author(s):  
Sandra Schumacher ◽  
Susanna Mayer ◽  
Constanze Sproll ◽  
Dirk Lachenmeier ◽  
Thomas Kuballa

1997 ◽  
Vol 21 (6) ◽  
pp. 492-497 ◽  
Author(s):  
S. J. Salamone ◽  
Z. Li ◽  
A. J. McNally ◽  
S. Vitone ◽  
R. S. Wu


PLoS ONE ◽  
2017 ◽  
Vol 12 (8) ◽  
pp. e0182767 ◽  
Author(s):  
Matthias Rzeznik ◽  
Mohamed Nawfal Triba ◽  
Pierre Levy ◽  
Sébastien Jungo ◽  
Eliot Botosoa ◽  
...  


1987 ◽  
Vol 80 (8) ◽  
pp. 495-498 ◽  
Author(s):  
D B Sprague ◽  
D G Gadian ◽  
S R Williams ◽  
E Proctor ◽  
A W Goode

Hind limb skeletal muscle was studied in vivo in a rat trauma model using nuclear magnetic resonance (NMR) spectroscopy. The model used was a 25% body surface area, full-thickness burn administered under anaesthesia. Two groups of six rats were studied. Weight loss was observed in the experimental group whilst the control group continued to gain weight. Concentration ratios involving intramyocellular phosphocreatine (PCr), creatine (Cr), adenosine triphosphate (ATP), inorganic phosphate (Pi), anserine (Ans) and taurine (Tau) were measured. No change in the ratios of PCr/Pi, PCr/ATP, Ans/PCr + Cr and Tau/PCr + Cr were seen between the two groups. Intracellular pH was the same in the two groups. NMR spectroscopy in vivo gives values of Pi and PCr that differ from those obtained by conventional techniques. NMR values are probably more accurate as no degradation occurs during measurement, the measurements being repeatable and noninvasive.





1977 ◽  
Vol 55 (18) ◽  
pp. 3298-3303 ◽  
Author(s):  
A. N. Thakore ◽  
A. C. Oehlschlager

Arguments based on 13C and 1H nmr spectroscopy are presented to elucidate the structures of 3,4,5- and 4,5,6-trichloroguaiacol, 12- and 14-monochlorodehydroabietic acid, and 12,14-dichlorodehydroabietic acid. These compounds were recently isolated and tentatively identified as major components toxic to fish in kraft mill caustic extraction effluents.



2015 ◽  
Vol 77 (33) ◽  
Author(s):  
Nurul Shahfizaa ◽  
Maulidiani Maulidiani ◽  
Hasnah Osman ◽  
Tang T. Hock ◽  
Khozirah Shaari ◽  
...  

Dengue is the most important human viral disease transmitted by arthropod vectors and over half of the world's populations live in areas at risk of infection. The severity of the infection depends on the form of the disease, which can be symptomatic or asymptomatic. Currently there is neither specific treatment nor vaccine to tackle this emerging disease. Metabolomics applied in this study, aimed to provide a global snapshot of all small-molecule metabolites in urine as biological sample of choice to more focused studies of metabolism to distinguish between healthy and dengue infected subjects. Fifty-two patients diagnosed with dengue fever at Penang General Hospital and fourty-three healthy individuals were recruited in this study. 1H-nuclear magnetic resonance (NMR) spectroscopy combined with multivariate analysis (MVA) methods such as principal component analysis (PCA), partial least square discriminant analysis (PLS-DA) and orthogonal PLS-DA (OPLS-DA) were employed for statistical data exploration. The model score plot results showed that all three MVAs showed very good spatial distributions with clear clusters/grouping between healthy individuals and dengue infected individuals. Also, statistically, the PLS-DA and OPLS-DA models had high reproducibility and predictivity values, > 0.5. In conclusion, this study established the potential of using a combination of 1H NMR spectroscopy and multivariate data analyses in differentiating healthy and non-healthy individuals, based on obtained score plots reflecting the metabolites pertubation, where spectral features contributing most to variation or separation are identified for further analysis.



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