scholarly journals Increased visceral adipose tissue in clear cell renal cell carcinoma with and without peritumoral collateral vessels

2020 ◽  
Vol 93 (1112) ◽  
pp. 20200334
Author(s):  
Federico Greco ◽  
Luigi Giuseppe Quarta ◽  
Rosario Francesco Grasso ◽  
Bruno Beomonte Zobel ◽  
Carlo Augusto Mallio
Keyword(s):  
Renal Cell Carcinoma ◽  
Adipose Tissue ◽  
Visceral Adipose Tissue ◽  
Renal Cell ◽  
Clear Cell ◽  
Control Group ◽  
Cell Renal Cell Carcinoma ◽  
Collateral Vessels ◽  
Visceral Adipose ◽  
And Control

Objective: The excessive amount of adipose tissue, mainly visceral, determines adiposopathy. With respect to oncogenesis, visceral adipose tissue (VAT) releases secretes adipokines, proinflammatory citokines and growth factors, considered mediating molecules in the development of obesity-related tumors. In this study, we quantify VAT in male patients with clear cell renal cell carcinoma (ccRCC) subgrouped according to the presence or absence of peritumoral collateral vessels. Methods: in this retrospective study, we enrolled 141 male caucasian patients divided into 2 groups: the ccRCC group (n = 106) composed of patients with ccRCC and control group (n = 35). The ccRCC group was further divided into two subgroups: the ccRCCa subgroup which showed absence of collateral vessels (n = 48) and ccRCCp subgroup with collateral vessels (n = 58). Total adipose tissue (TAT) area, VAT area and subcutaneous adipose tissue (SAT) area were measured in the groups and subgroups. VAT/SAT ratio was calculated for each subject. Results: Statistically significant differences were obtained between ccRCC group and control group for TAT area (p < 0.005), VAT area (p < 0.005) and SAT area (p = 0.01). Between ccRCCa subgroup and control group for TAT area (p < 0.001), VAT area (p = 0.005) and SAT area (p = 0.001). Between ccRCCp subgroup and control group for TAT area (p = 0.01) and VAT area (p = 0.01). Conclusion: This study confirms the increase of abdominal, especially visceral, adipose tissue in ccRCC patients and demonstrates a significant VAT accumulation in both categories of patients with and without peritumoral collateral vessels. Advances in knowledge: Visceral adiposity is present in patients with ccRCC regardless the presence of peritumoral collateral vessels, with surprisingly stronger results in the ccRCCa subgroup.

Increased visceral adipose tissue in male patients with clear cell renal cell carcinoma

2018 ◽  
Vol 7 (4) ◽  
pp. 132 ◽  
Author(s):  
Federico Greco ◽  
Vincenzo Cirimele ◽  
CarloAugusto Mallio ◽  
BrunoBeomonte Zobel ◽  
RosarioFrancesco Grasso
Keyword(s):  
Renal Cell Carcinoma ◽  
Adipose Tissue ◽  
Cell Carcinoma ◽  
Visceral Adipose Tissue ◽  
Renal Cell ◽  
Clear Cell ◽  
Cell Renal Cell Carcinoma ◽  
Male Patients ◽  
Visceral Adipose

Increased visceral adipose tissue in male patients with non-clear cell renal cell carcinoma

2020 ◽  
Vol 125 (6) ◽  
pp. 538-543 ◽  
Author(s):  
Federico Greco ◽  
Carlo Augusto Mallio ◽  
Raffaele Grippo ◽  
Laura Messina ◽  
Silvia Vallese ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Adipose Tissue ◽  
Cell Carcinoma ◽  
Visceral Adipose Tissue ◽  
Renal Cell ◽  
Clear Cell ◽  
Cell Renal Cell Carcinoma ◽  
Male Patients ◽  
Visceral Adipose

Fatty acid synthase (FASN), a novel signature for visceral adipose tissues, regulates proliferation, migration and predicts prognosis of clear cell renal cell carcinoma

2020 ◽  
Author(s):  
Wen-Hao Xu ◽  
Aihetaimujiang Anwaier ◽  
Hong-Kai Wang ◽  
Jun Wang ◽  
Wen-Kai Zhu ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Adipose Tissue ◽  
Mrna Expression ◽  
Renal Cell ◽  
Clear Cell ◽  
Cell Renal Cell Carcinoma ◽  
Normal Tissues ◽  
Hazard Ratios ◽  
And Migration ◽  
Visceral Adipose

Abstract Background: Growing evidence has indicated obesity one of the important etiological indicators of clear cell renal cell carcinoma (ccRCC). This study aims to investigated FASN mRNA expression in anthropometric adipose tissue, and elucidate prognostic value and potential functions in ccRCC patients. Methods: Transcriptional expression profiles was obtained from 380 paired ccRCC and adjacent normal tissues from Fudan University Shanghai Cancer Center (FUSCC) and 533 ccRCC samples from the Cancer Genome Atlas (TCGA) cohort. The visceral adipose tissue (VAT) and the subcutaneous adipose tissue (SAT) at the level of the umbilicus were measured using magnetic resonance imaging. The Kaplan-Meier method and log-rank test, with cooperation from Cox regression analysis, were used in survival analysis. Subsequently, we transiently transfected FASN plasmid into A498 and 786O cells, and investigated the role of FASN in ccRCC cell proliferation, apoptosis and migration in vitro. Related hub gens, functional annotations and significant signal pathways were predicted using integrated bioinformatics. Results: FASN mRNA expression was significantly higher in tumor than normal tissues in 913 ccRCC patients from FUSCC and TCGA cohorts. In addition, increased FASN mRNA expression was significantly relevant to advanced T stage ( p <0.001), N stage ( p =0.019), and AJCC stage ( p =0.002). Pearson’s correlation coefficient indicated that FASN amplification positively correlated with VAT% ( r =0.772, p <0.001). VAT% was significantly correlated with poor PFS and OS, with hazard ratios of 2.066 (1.113-7.261, p =0.028) and 2.773 (1.168-8.974, p =0.023) in FUSCC. Meanwhile, ccRCC patients with elevated FASN expression significantly responded for poor PFS and OS, with hazard ratios of 1.529 (1.135-2.061, p =0.005) and 1.450 (1.030-2.041, p =0.033). Next, after transfection efficiency was verified in A498 and 786O cells, we found transient inhibition and overexpression of FASN significantly regulates cells proliferation and migration abilities, and inhibition of FASN displayed higher apoptotic rate in ccRCC cells. Conclusion: In conclusion, this study demonstrated that FASN mRNA expression is positively related with aggressive proliferation, migration progression and predicts prognosis of ccRCC. In addition, we first reveal that elevated FASN mRNA expression is significantly correlated with abdominal obesity distribution, especially VAT%, which also is a significant predictor of poor prognosis in patients with ccRCC.

scholarly journals Subcutaneous Adipose Tissue Reduction in Patients with Clear Cell Renal Cell Carcinoma and Peritumoral Collateral Vessels: A Retrospective Observational Study

2021 ◽  
Vol 11 (13) ◽  
pp. 6076
Author(s):  
Federico Greco ◽  
Luigi Giuseppe Quarta ◽  
Aldo Carnevale ◽  
Melchiore Giganti ◽  
Rosario Francesco Grasso ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Adipose Tissue ◽  
Cell Carcinoma ◽  
Renal Cell ◽  
Subcutaneous Adipose Tissue ◽  
Clear Cell ◽  
Cell Renal Cell Carcinoma ◽  
Significant Difference ◽  
Collateral Vessels ◽  
Subcutaneous Adipose

Background: peritumoral collateral vessels adjacent to renal cell carcinoma (RCC) can be encountered in clinical practice. Cancer cachexia is defined as a decrease of adipose and skeletal muscle tissues. In this study we evaluated, using a quantitative CT imaging-based approach, the distribution of abdominal adipose tissue in clear cell RCC (ccRCC) male patients with and without collateral vessels. Methods: between November 2019 and February 2020, in this retrospective study we enrolled 106 ccRCC male Caucasian patients divided into two groups: a ccRCCa group without collateral vessels (n = 48) and a ccRCCp group with collateral vessels (n = 58). The total adipose tissue (TAT), visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) areas were measured in both groups. Moreover, the VAT/SAT ratio was calculated for each subject. Results: a statistically significant difference between the two groups was found in the SAT area (p < 0.05), while no significant differences were found in the TAT area, VAT area and VAT/SAT ratio. Conclusion: this study demonstrates a reduction of SAT in ccRCC patients with peritumoral collateral vessels.

scholarly journals Elevated CD36 expression correlates with increased visceral adipose tissue and predicts poor prognosis in ccRCC patients.

2019 ◽  
Vol 37 (7_suppl) ◽  
pp. 571-571
Author(s):  
Wen-Hao Xu ◽  
Yuan-Yuan Qu ◽  
Jun Wang ◽  
Hai-Liang Zhang ◽  
Dingwei Ye
Keyword(s):  
Renal Cell Carcinoma ◽  
Adipose Tissue ◽  
Cell Carcinoma ◽  
Mrna Expression ◽  
Correlation Coefficient ◽  
Visceral Adipose Tissue ◽  
Renal Cell ◽  
Poor Prognosis ◽  
Cd36 Expression ◽  
Visceral Adipose

571 Background: Growing evidence has proved obesity one of the confirmed important etiologic indicators for renal cell carcinoma (RCC). CD36 is underpinned to be involved in adipose absorption, but its role in clear cell renal cell carcinoma (ccRCC) remains unclear. This study aimed to investigate the mRNA expression of CD36 in anthropometric measures of adipose tissue and defining its value in predicting prognosis in ccRCC patients. Methods: Real-Time qPCR was detected from 367 paired ccRCC and adjacent normal tissues. Distributions of categorical clinical-pathological data together with levels of CD36 expression were compared with χ2-test. Subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) were measured by MRI. Pearson’s correlation coefficient was utilized to quantify relations between body mass index (BMI), VAT%, SAT and CD36 expression respectively. Cox regression analysis were developed to address the influence of independent factors on prognostic value. Gene Set Enrichment Analysis (GSEA) was performed to select related genes and pathways from TCGA database. Results: In the current study, we demonstrated that CD36 mRNA was highly expressed in ccRCC tissues, and was found significantly increased in patients with advanced TNM stage ( p= 0.003, p< 0.001, p< 0.001), and high VAT% ( p= 0.004). Pearson’s correlation coefficient indicated CD36 amplification positively correlated with BMI ( r= 0.117, p= 0.025), VAT% ( r= 0.465, p< 0.001), while negatively with SAT ( r= -0.296, p= 0.002). Furthermore, ccRCC patients with elevated CD36 expression held shorter PFS and OS, with HR of 4.873 (3.300-7.196, p< 0.001) and 4.610 (2.956-7.189, p< 0.001). In 104 cases whose MRI scans were available, VAT was significantly correlated with poor PFS and OS. Significant genes were obtained from GSEA, and CD36 was found involved in the most significant pathways including fatty acid metabolism, angiogenesis and TGF-β signaling pathways. Conclusions: In conclusion, our study first reveal that elevated CD36 mRNA expression is positively correlated to distribution of abdominal adipose, particularly VAT%, which, in addition, notably predicts poor prognosis in ccRCC patients.

Effect of EH domain containing protein 2 on the biological behavior of clear cell renal cell carcinoma

2019 ◽  
Vol 38 (8) ◽  
pp. 927-937 ◽  
Author(s):  
C Liu ◽  
S Liu ◽  
L Wang ◽  
Y Wang ◽  
Y Li ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Western Blot ◽  
Cell Carcinoma ◽  
Renal Cell ◽  
Clear Cell ◽  
Control Group ◽  
Cell Renal Cell Carcinoma ◽  
Invasion And Migration ◽  
Eh Domain ◽  
And Migration

To investigate the effects of EH domain containing protein 2 (EHD2) on clear cell renal cell carcinoma (ccRCC) and provide new insights for the clinical treatment of rental cancer. Forty patients (26 males and 14 females, 62.4 ± 5.7 years old) with ccRCC were selected from January 2015 to December 2016 to serve as research subjects in this study. The EHD2 protein expression in the tumor tissues and adjacent healthy tissues of ccRCC patients were detected by Western Blot assay. The cells of ccRCC cell lines RLC-310 and 786-O were divided into normal control group (control), no-load control group (pLV), EHD2 overexpression group (pLV-EHD2), and EHD2 interference group (pLV-siEHD2). The expression levels of EHD2 protein in each group of cells were detected by western blot. The cell proliferation was detected by Cell Counting Kit-8 (CCK-8) assay. Wound healing assay was performed to check the cell migration ability. Transwell invasion assay was used to detect the cell invasion ability. Cell apoptosis was detected by flow cytometry. The expression level of EHD2 was significantly increased in pLV-EHD2 group and decreased in pLV-siEHD2 group compared with control group and pLV-siEHD2 group, indicating the successfully established EHD2 overexpression cell line and EHD2 RNA interference cell line. EHD2 overexpression enhanced the proliferation, invasion, and migration but inhibited the apoptosis of ccRCC cells, while EHD2 interference showed opposite functions. EHD2 interference can inhibit the development of ccRCC by inhibiting the proliferation, invasion, and migration, and EHD2 can potentially serve as a molecular target for the clinical treatment of ccRCC.

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