scholarly journals Haplotype variation in the Physa acuta group (Basommatophora): genetic diversity and distribution in Serbia

2016 ◽  
Vol 17 (1) ◽  
pp. 292 ◽  
Author(s):  
M. J. RAKOVIĆ ◽  
M. B. RAKOVIĆ ◽  
A. M. PETROVIĆ ◽  
N. Z. POPOVIĆ ◽  
J. A. ĐUKNIĆ ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
North America ◽  
Abundant Species ◽  
Separate Species ◽  
High Genetic Diversity ◽  
Freshwater Gastropods ◽  
Multiple Introductions ◽  
Physa Acuta ◽  
Haplotype Variation ◽  
Migrating Birds

The genus Physa (= Physella) includes the most abundant and diverse freshwater gastropods native to North America. Due to their invasive nature many species occur throughout the world. The most abundant species, Physa acuta, has been introduced to Europe, Africa, Asia and Australia by human commerce and migrating birds. This species is widely distributed throughout Serbia. The aim of this study was to explore the genetic diversity of P. acuta from Serbia, and to determine the evolutionary relationships among native Physidae populations from North America, Mexico and Cuba and populations from Europe using sequences of the mitochondrial 16S rDNA gene. The ML (Maximum Likelihood) tree revealed two clades within Physidae, and two clades that correspond to the families Planorbidae and Lymnaeidae. In the Physidae clade there are two separate clades: one includes the species Physa spelunca, and the second includes samples of P. acuta. We determined three different haplotypes within specimens from Serbia. One haplotype is genetically closest to species Physa heterostrofa (synonym of P. acuta) from Philadelphia, while the other two are very close to P. acuta specimens from New Mexico. Together with other samples our findings corroborate the notion that we are dealing with one panmictic population of P. acuta and not with several separate species, despite the high genetic diversity between and among the populations. Our results indicate that in the same population in Serbia, there is high genetic distance between samples. Despite the small number of analyzed samples, our findings point to multiple introductions of P. acuta from different locations in America.

scholarly journals Patterns of Genetic Diversity in the Globally Invasive Species Wild Parsnip (Pastinaca sativa)

2015 ◽  
Vol 8 (4) ◽  
pp. 415-429 ◽  
Author(s):  
Tania Jogesh ◽  
Rhiannon Peery ◽  
Stephen R. Downie ◽  
May R. Berenbaum
Keyword(s):  
Genetic Diversity ◽  
Invasive Species ◽  
North America ◽  
Nuclear Ribosomal Dna ◽  
High Genetic Diversity ◽  
Wild Parsnip ◽  
Multiple Introductions ◽  
Cpdna Markers ◽  
Global Invasion ◽  
Accidental Introduction

AbstractWild parsnip is an invasive species with a global distribution in temperate climates. Parsnips are native to Eurasia and have been cultivated for more than five centuries. It is unclear whether the global invasion of this species is a consequence of escape from cultivation or the accidental introduction of a Eurasian wild subspecies. In this study, we used nuclear ribosomal DNA internal transcribed spacer (ITS) and chloroplast DNA (cpDNA) markers to evaluate the genetic structure of wild parsnip in its native range (Europe) and in three distinct geographic regions where it is considered invasive: eastern North America, western North America, and New Zealand. We also compared wild and cultivated parsnips to determine whether they are genetically distinct. From 112 individuals, we recovered 14 ITS and 27 cpDNA haplotypes. One ITS haplotype was widespread; few haplotypes were rare singletons. In contrast, at least two lineages of cpDNA haplotypes were recovered, with several novel haplotypes restricted to Europe. Cultivated parsnips were not genetically distinct from wild parsnips, and numerous wild parsnip populations shared haplotypes with cultivars. High genetic diversity was recovered in all three regions, suggesting multiple introductions.

scholarly journals Highly Clonal Structure and Abundance of One Haplotype Characterise the Diplodia sapinea Populations in Europe and Western Asia

2021 ◽  
Vol 7 (8) ◽  
pp. 634
Author(s):  
Kalev Adamson ◽  
Marili Laas ◽  
Kathrin Blumenstein ◽  
Johanna Busskamp ◽  
Gitta J. Langer ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
North America ◽  
Opportunistic Pathogen ◽  
Pcr Primers ◽  
High Genetic Diversity ◽  
Multiple Introductions ◽  
Single Tree ◽  
Western Asia ◽  
Species Specific ◽  
Pinus Spp

Diplodia sapinea is a cosmopolitan endophyte and opportunistic pathogen having occurred on several conifer species in Europe for at least 200 years. In Europe, disease outbreaks have increased on several Pinus spp. in the last few decades. In this study, the genetic structure of the European and western Asian D. sapinea population were investigated using 13 microsatellite markers. In total, 425 isolates from 15 countries were analysed. A high clonal fraction and low genetic distance between most subpopulations was found. One single haplotype dominates the European population, being represented by 45.3% of all isolates and found in nearly all investigated countries. Three genetically distinct subpopulations were found: Central/North European, Italian and Georgian. The recently detected subpopulations of D. sapinea in northern Europe (Estonia) share several haplotypes with the German subpopulation. The northern European subpopulations (Latvia, Estonia and Finland) show relatively high genetic diversity compared to those in central Europe suggesting either that the fungus has existed in the North in an asymptomatic/endophytic mode for a long time or that it has spread recently by multiple introductions. Considerable genetic diversity was found even among isolates of a single tree as 16 isolates from a single tree resulted in lower clonal fraction index than most subpopulations in Europe, which might reflect cryptic sexual proliferation. According to currently published allelic patterns, D. sapinea most likely originates from North America or from some unsampled population in Asia or central America. In order to enable the detection of endophytic or latent infections of planting stock by D. sapinea, new species-specific PCR primers (DiSapi-F and Diplo-R) were designed. During the search for Diplodia isolates across the world for species specific primer development, we identified D. africana in California, USA, and in the Canary Islands, which are the first records of this species in North America and in Spain.

scholarly journals Distribution and phylogenetic diversity of Anopheles species in malaria endemic areas of Honduras in an elimination setting

2020 ◽  
Author(s):  
Denis Escobar ◽  
Krisnaya Ascencio ◽  
Andrés Ortiz ◽  
Adalid Palma ◽  
Gustavo Fontecha
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analyses ◽  
Public Health Problem ◽  
Abundant Species ◽  
Malaria Vectors ◽  
Morphological Identification ◽  
High Genetic Diversity ◽  
Homogeneous Population ◽  
Molecular Approaches ◽  
Endemic Areas

Abstract Background: Anopheles mosquitoes are the vectors of malaria, one of the most important infectious diseases in the tropics. More than 500 Anopheles species have been described worldwide, and more than 30 are considered a public health problem. In Honduras, information on the distribution of Anopheles spp. and its genetic diversity is scarce. This study aimed to describe the distribution and genetic diversity of Anopheles mosquitoes in Honduras. Methods: Mosquitoes were captured in 8 locations in 5 malaria endemic departments during 2019. Two collection methods were used. Adult anophelines were captured outdoors using CDC light traps and by aspiration of mosquitoes at rest. The morphological identification was performed using taxonomic keys. Genetic analyses included the sequencing of a partial region of the cytochrome oxidase I gene (COI) and the ribosomal internal transcribed spacer 2 (ITS2). Results: A total of 1320 anophelines were collected and identified through morphological keys. Seven Anopheles species were identified. Anopheles albimanus was the most widespread and abundant species (74.02%). To confirm the morphological identification of the specimens, 175 and 122 sequences were obtained for COI and ITS2 respectively. Both markers confirmed the morphological identification. COI showed a greater nucleotide diversity than ITS2 in all species. High genetic diversity was observed within the populations of An. albimanus while An. darlingi proved to be a highly homogeneous population. Phylogenetic analyses revealed clustering patterns in An. darlingi and An. neivai in relation to specimens from South America. New sequences for An. crucians, An. vestitipennis, and An. neivai are reported in this study.Conclusions: Here we report the distribution and genetic diversity of Anopheles species in endemic areas of malaria transmission in Honduras. According to our results, both taxonomic and molecular approaches are useful tools in the identification of anopheline mosquitoes. However, both molecular markers differ in their ability to detect intraspecific genetic diversity. These results provide supporting data for a better understanding of the distribution of malaria vectors in Honduras.

A discreet invasion: distribution, origins, and expansion of the European slug complex Arion subfuscus s.l. in Quebec

2018 ◽  
Vol 96 (4) ◽  
pp. 325-331 ◽  
Author(s):  
Érik L’Heureux ◽  
Bernard Angers
Keyword(s):  
North America ◽  
Exotic Species ◽  
Human Activities ◽  
Single Species ◽  
Abundant Species ◽  
The United States ◽  
Accurate Identification ◽  
Multiple Introductions ◽  
Species Complexes ◽  
Arion Subfuscus

Accurate identification of species and their geographic origins is crucial to assess the impacts of exotic species. This is particularly relevant when multiple introductions are suspected. Species complexes are therefore problematic, as different species can be treated as a single taxon. The highly diversified European complex Arion subfuscus (Draparnaud, 1805) s.l. is abundant throughout northeastern North America. However, surveys performed in the United States have reported a low mitochondrial diversity associated with a single species. To assess whether regions characterized by different human activities harbor different diversities, the taxonomic identities, distributions, and origins of the species of this complex in Quebec (Canada) were determined. The mitochondrial 16S rDNA gene was analyzed in 526 specimens from 69 locations. Both Arion fuscus (O.F. Müller, 1774) and Arion subfuscus (Draparnaud, 1805) s. str. (lineages S1 and S2) were detected. Arion fuscus was the most abundant species, contrasting with previous studies conducted in North America. An explosive range expansion occurred during the last 50 years. Moreover, the presence of different haplotypes confirmed multiple introductions of these species. These results emphasize the importance of not considering an exotic species complex as a single taxon, especially in regions with different human activities.

scholarly journals Highly clonal structure and abundance of one haplotype characterize the Diplodia sapinea populations in Europe and western Asia

2021 ◽  
Author(s):  
Kalev Adamson ◽  
Marili Laas ◽  
Kathrin Blumenstein ◽  
Johanna Busskamp ◽  
Gitta Langer ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
North America ◽  
Central Europe ◽  
Opportunistic Pathogen ◽  
Pcr Primers ◽  
First Record ◽  
German Population ◽  
Multiple Introductions ◽  
Western Asia ◽  
Pinus Spp

Diplodia sapinea is a cosmopolitan endophyte and opportunistic pathogen occurring on several conifer species in Europe for at least 200 years. In Europe, disease outbreaks have increased on several Pinus spp. in the last few decades. In this study, the genetic structure of the European D. sapinea population was investigated using thirteen microsatellite markers. In total, 425 isolates from 15 countries were analysed. A high clonal fraction and low genetic distance between most populations was found. One single haplotype dominates the European population, being represented by 44% of all isolates and found in nearly all investigated countries. Three genetically distinct subpopulations were found: Central/North European, Italian and Georgian. The recently detected populations of D. sapinea in northern Europe (Latvia, Estonia and Finland) share several haplotypes with the German population, suggesting introduction from Central Europe. The northern European populations show similar genetic diversity to those in Central Europe suggesting either that the fungus has existed in the North in an asymptomatic mode for a long time or that it has spread recently by multiple introductions. Although this fungus reproduces predominantly asexually, considerable genetic diversity was found even among isolates of a single tree. According to currently published allelic patterns, D. sapinea most likely originates from North America. In order to enable the detection of endophytic or latent infections of planting stock by D. sapinea, new species-specific PCR primers were designed. During the search for Diplodia isolates, we identified D. africana in California, USA, which is the first record of this species in North America.

scholarly journals Looks can be deceiving: the deceptive milkcaps (Lactifluus, Russulaceae) exhibit low morphological variance but harbour high genetic diversity

IMA Fungus ◽  
2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Lynn Delgat ◽  
Glen Dierickx ◽  
Serge De Wilde ◽  
Claudio Angelini ◽  
Eske De Crop ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
North America ◽  
Species Complex ◽  
Molecular Study ◽  
Similar Species ◽  
High Genetic Diversity ◽  
Its1 Sequence ◽  
Neotropical Species ◽  
Species Complexes ◽  
Thin Walled

Abstract The ectomycorrhizal genus Lactifluus is known to contain many species complexes, consisting of morphologically very similar species, which can be considered cryptic or pseudocryptic. In this paper, a thorough molecular study is performed of the clade around Lactifluus deceptivus (originally described by Peck from North America) or the deceptive milkcaps. Even though most collections were identified as L. deceptivus, the clade is shown to contain at least 15 species, distributed across Asia and America, indicating that the L. deceptivus clade represents a species complex. These species are morphologically very similar and are characterized by a tomentose pileus with thin-walled hyphae and a velvety stipe with thick-walled hyphae. An ITS1 sequence was obtained through Illumina sequencing for the lectotype of L. deceptivus, dating from 1885, revealing which clade represents the true L. deceptivus. In addition, it is shown that three other described species also belong to the L. deceptivus clade: L. arcuatus, L. caeruleitinctus and L. mordax, and molecularly confirmed that L. tomentoso-marginatus represents a synonym of L. deceptivus. Furthermore, two new Neotropical species are described: Lactifluus hallingii and L. domingensis.

scholarly journals Geographic Distribution and Genetic Diversity of Three Ophiosphaerella Species that Cause Spring Dead Spot of Bermudagrass

Plant Disease ◽  
1999 ◽  
Vol 83 (12) ◽  
pp. 1160-1166 ◽  
Author(s):  
Henry C. Wetzel ◽  
Daniel Z. Skinner ◽  
Ned A. Tisserat
Keyword(s):  
United States ◽  
Genetic Diversity ◽  
North America ◽  
Fragment Length Polymorphism ◽  
Kentucky Bluegrass ◽  
Abundant Species ◽  
Golf Courses ◽  
Asexual Propagation ◽  
Spring Dead Spot ◽  
Sexual Recombination

The distribution of three Ophiosphaerella spp. that cause spring dead spot (SDS) of bermudagrass was studied by systematically sampling two golf courses in Oklahoma and one in Kansas. O. herpotricha was isolated from all three locations and was the most abundant species. It was the only SDS pathogen found at Jenks, Oklahoma. O. korrae was isolated from Afton, Oklahoma, and Independence, Kansas, whereas O. narmari was only detected in samples from Afton. This is the first report of all three Ophiosphaerella species on bermudagrass at the same location. Amplified fragment length polymorphism (AFLP) marker analysis was used to investigate inter- and intraspecific genetic diversity of Ophiosphaerella isolates from North America and Australia. A majority of the O. herpotricha and O. narmari isolates from Afton were distinct haplotypes, suggesting that sexual recombination was occurring within the population. Conversely, the presence of multiple isolates of O. herpotricha and O. narmari with the same haplotype also indicated that asexual propagation was occurring. The genetic diversity among O. herpotricha isolates from Afton was not distinctly different from that of isolates collected throughout the southern United States. In contrast, O. narmari isolates from Afton were distinct from those collected in Australia. The genetic diversity in O. korrae was markedly different than that in the other Ophiosphaerella spp. The population at Afton was dominated by just a few haplotypes, and these were nearly identical to isolates collected from bermudagrass and Kentucky bluegrass throughout western, central, and northern North America. However, O. korrae isolates collected in the southeastern United States were only distantly similar to other North American isolates.

scholarly journals Distribution of Anopheles species in malaria endemic areas of Honduras in an elimination setting

2020 ◽  
Author(s):  
Denis Escobar ◽  
Krisnaya Ascencio ◽  
Andrés Ortiz ◽  
Adalid Palma ◽  
Gustavo Fontecha
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analyses ◽  
Public Health Problem ◽  
Abundant Species ◽  
Malaria Vectors ◽  
Morphological Identification ◽  
High Genetic Diversity ◽  
Homogeneous Population ◽  
Molecular Approaches ◽  
Endemic Areas

Abstract Background: Anopheles mosquitoes are the vectors of malaria, one of the most important infectious diseases in the tropics. More than 500 Anopheles species have been described worldwide, and more than 30 are considered a public health problem. In Honduras, information on the distribution of Anopheles spp. and its genetic diversity is scarce. This study aimed to update information on the diversity of Anopheles mosquitoes in Honduras with a morphological and molecular approach. Methods: Mosquitoes were captured in 8 locations in 5 malaria endemic departments during 2019. Two collections methods were used. Adult anophelines were captured outdoors using CDC light traps and by aspiration of mosquitoes at rest. The morphological identification was performed using taxonomic keys. Genetic analyses included the sequencing of a partial region of the cytochrome oxidase I gene (COI) and the ribosomal internal transcribed spacer 2 (ITS2). Results: A total of 1320 anophelines were collected and identified through morphological keys. Seven Anopheles species were identified. An. albimanus was the most widespread and abundant species (74.02%). To confirm the morphological identification of the specimens, 175 and 122 sequences were obtained for COI and ITS2 respectively. Both markers confirmed the morphological identification. COI showed a greater nucleotide diversity than ITS2 in all species. High genetic diversity was observed within the populations of An. albimanus while An. darlingi proved to be a highly homogeneous population. Phylogenetic analyses revealed clustering patterns in An. darlingi and An. neivai in relation to specimens from South America. New sequences for An. crucians, An. vestitipennis, and An. neivai are reported in this study. Conclusions: Here we report the distribution of Anopheles species in endemic areas of malaria in Honduras. According to our results, both taxonomic and molecular approaches are useful tools in the identification of anopheline mosquitoes. However, both molecular markers differ in their ability to detect intraspecific genetic diversity. These results provide supporting data for a better understanding of the distribution of malaria vectors in Honduras.

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